E3泛素连接酶MUL1抗体

E3泛素连接酶MUL1抗体 

『别名』E3 ubiquitin-protein ligase MUL1; C1orf166; E3 ubiquitin ligase; E3 ubiquitin protein ligase MUL1; GIDE; Growth inhibition and death E3 ligase; MAPL; Mitochondrial anchored protein ligase; Mitochondrial ubiquitin ligase activator of NFKB 1; MUL1; MULAN; Putative NF kappa B activating protein 266; RING finger protein 218; RNF218; RP23-25C1.10-002; MUL1_HUMAN. 

『浓度』E3泛素连接酶MUL1抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

E3泛素连接酶MUL1抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

E3泛素连接酶MUL1抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAC895Hu01 分拣蛋白1(SORT1)多克隆抗体 Polyclonal Antibody to Sortilin 1 (SORT1)

PAH133Hu01 抗药蛋白(SRI)多克隆抗体 Polyclonal Antibody to Sorcin (SRI)

PAB495Bo01 山梨醇脱氢酶(SDH)多克隆抗体 Polyclonal Antibody to Sorbitol Dehydrogenase (SDH)

PAB495Hu01 山梨醇脱氢酶(SDH)多克隆抗体 Polyclonal Antibody to Sorbitol Dehydrogenase (SDH)

PAB495Ra01 山梨醇脱氢酶(SDH)多克隆抗体 Polyclonal Antibody to Sorbitol Dehydrogenase (SDH)

PAK520Ge01 山梨酸(SA)多克隆抗体 Polyclonal Antibody to Sorbic Acid (SA)

PAA592Hu01 生长抑素(SST)多克隆抗体 Polyclonal Antibody to Somatostatin (SST)

PAA592Mu01 生长抑素(SST)多克隆抗体 Polyclonal Antibody to Somatostatin (SST)

PAA592Ra01 生长抑素(SST)多克隆抗体 Polyclonal Antibody to Somatostatin (SST)

PAA592Po01 生长抑素(SST)多克隆抗体 Polyclonal Antibody to Somatostatin (SST)

PAA592Mu02 生长抑素(SST)多克隆抗体 Polyclonal Antibody to Somatostatin (SST)

PAA592Hu02 生长抑素(SST)多克隆抗体 Polyclonal Antibody to Somatostatin (SST)

PAE419Hu01 溶质载体家族7成员9(SLC7A9)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 7, Member 9 (SLC7A9)

PAE410Ra01 溶质载体家族7成员11(SLC7A11)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 7, Member 11 (SLC7A11)

PAE486Hu01 溶质载体家族39成员6(SLC39A6)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 39, Member 6 (SLC39A6)

PAG577Mu01 溶质载体家族30成员6(SLC30A6)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 6 (SLC30A6)

PAG577Mu02 溶质载体家族30成员6(SLC30A6)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 6 (SLC30A6)

PAG576Mu01 溶质载体家族30成员5(SLC30A5)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 5 (SLC30A5)

PAG576Mu02 溶质载体家族30成员5(SLC30A5)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 5 (SLC30A5)

PAG575Hu01 溶质载体家族30成员3(SLC30A3)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 3 (SLC30A3)

PAG575Mu01 溶质载体家族30成员3(SLC30A3)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 3 (SLC30A3)

PAG575Mu02 溶质载体家族30成员3(SLC30A3)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 3 (SLC30A3)

PAN188Mu01 溶质载体家族30成员10(SLC30A10)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 10 (SLC30A10)

PAN188Mu02 溶质载体家族30成员10(SLC30A10)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 10 (SLC30A10)

PAE487Mu01 溶质载体家族30成员1(SLC30A1)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 1 (SLC30A1)

PAE487Mu02 溶质载体家族30成员1(SLC30A1)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 1 (SLC30A1)

PAE487Ra01 溶质载体家族30成员1(SLC30A1)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30, Member 1 (SLC30A1)

PAE490Hu01 溶质载体家族30成员8(SLC30A8)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30 Member 8 (SLC30A8)

PAE490Hu02 溶质载体家族30成员8(SLC30A8)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30 Member 8 (SLC30A8)

PAE489Mu01 溶质载体家族30成员7(SLC30A7)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30 Member 7 (SLC30A7)

PAE489Mu02 溶质载体家族30成员7(SLC30A7)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30 Member 7 (SLC30A7)

PAE488Mu01 溶质载体家族30成员4(SLC30A4)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30 Member 4 (SLC30A4)

PAE488Mu02 溶质载体家族30成员4(SLC30A4)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 30 Member 4 (SLC30A4)

PAE348Hu01 溶质载体家族3成员2(SLC3A2)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 3, Member 2 (SLC3A2)

PAE348Hu02 溶质载体家族3成员2(SLC3A2)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 3, Member 2 (SLC3A2)

PAE348Mu01 溶质载体家族3成员2(SLC3A2)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 3, Member 2 (SLC3A2)

PAE348Ra01 溶质载体家族3成员2(SLC3A2)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 3, Member 2 (SLC3A2)

PAE474Hu01 溶质载体家族26成员4(SLC26A4)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 26, Member 4 (SLC26A4)

PAG592Hu01 溶质载体家族16成员8(SLC16A8)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 16, Member 8 (SLC16A8)

PAE433Hu01 溶质载体家族16成员3(SLC16A3)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 16, Member 3 (SLC16A3)

PAE431Hu01 溶质载体家族16成员1(SLC16A1)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 16, Member 1 (SLC16A1)

PAE431Ra01 溶质载体家族16成员1(SLC16A1)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 16, Member 1 (SLC16A1)

PAE345Hu01 溶质载体家族1成员5(SLC1A5)多克隆抗体 Polyclonal Antibody to Solute Carrier Family 1, Member 5 (SLC1A5)

PAE413Ra01 钠/氢交换因子3(NHE3)多克隆抗体 Polyclonal Antibody to Sodium/Hydrogen Exchanger 3 (NHE3)

PAE411Hu01 钠/氢交换因子1(NHE1)多克隆抗体 Polyclonal Antibody to Sodium/Hydrogen Exchanger 1 (NHE1)

PAE382Hu01 钠/葡萄糖协同转运蛋白2(SGLT2)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 2 (SGLT2)

PAE382Ra01 钠/葡萄糖协同转运蛋白2(SGLT2)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 2 (SGLT2)

PAE381Hu01 钠/葡萄糖协同转运蛋白1(SGLT1)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 1 (SGLT1)

PAE381Mu01 钠/葡萄糖协同转运蛋白1(SGLT1)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 1 (SGLT1)

PAE381Mu02 钠/葡萄糖协同转运蛋白1(SGLT1)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 1 (SGLT1)

PAE385Hu01 钠碘运输蛋白(NIS)多克隆抗体 Polyclonal Antibody to Sodium Iodide Symporter (NIS)

PAE505Hu01 钠耦合中性氨基酸转运蛋白1(SNAT1)多克隆抗体 Polyclonal Antibody to Sodium Coupled Neutral Amino Acid Transporter 1 (SNAT1)

PAH163Hu01 NAP关联蛋白(SNAPAP)多克隆抗体 Polyclonal Antibody to SNAP Associated Protein (SNAPAP)

PAK089Hu01 Snail同源物1(SNAI1)多克隆抗体 Polyclonal Antibody to Snail Homolog 1 (SNAI1)

PAC856Hu01 平滑肌细胞分化特异性抗原(SMTN)多克隆抗体 Polyclonal Antibody to Smoothelin (SMTN)

PAC856Ra01 平滑肌细胞分化特异性抗原(SMTN)多克隆抗体 Polyclonal Antibody to Smoothelin E3泛素连接酶MUL1抗体(SMTN)

PAG152Hu01 小泛素相关修饰蛋白2(SUMO2)多克隆抗体 Polyclonal Antibody to Small Ubiquitin Related Modifier Protein 2 (SUMO2)

PAG154Hu01 小泛素相关修饰蛋白1(SUMO1)多克隆抗体 Polyclonal Antibody to Small Ubiquitin Related Modifier Protein 1 (SUMO1)

PAG154Mu01 小泛素相关修饰蛋白1(SUMO1)多克隆抗体 Polyclonal Antibody to Small Ubiquitin Related Modifier Protein 1 (SUMO1)