可溶性苹果酸脱氢酶抗体
『别名』Cytosolic malate dehydrogenase; Malate dehydrogenase 1, NAD (soluble); MDHA; MDHs; Soluble malate dehydrogenase; Malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1; Malate dehydrogenase 1 NAD (soluble); Malate dehydrogenase 2;可溶性苹果酸脱氢酶抗体 Malate dehydrogenase cytoplasmic; Malate dehydrogenase 2 NAD (mitochondrial); MDH 1; MDH 2; MDH; MDH1; MDH2; MDHA; MDHm; MDHs; MMDH; Mor 1; Mor 2; Mor1; MOR2; cytoplasmic; MDH s; mdh1; MDHC_HUMAN; MGC:1375; SMDH; Soluble malate dehydrogenase.
『浓度』1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
可溶性苹果酸脱氢酶抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
可溶性苹果酸脱氢酶抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAH197Hu01 上池蛋白(SBSN)多克隆抗体 Polyclonal Antibody to Suprabasin (SBSN)
PAB684Hu01 细胞因子信号转导抑制因子3(SOCS3)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3)
PAB684Mu01 细胞因子信号转导抑制因子3(SOCS3)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3)
PAB684Ra01 细胞因子信号转导抑制因子3(SOCS3)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3)
PAB684Mu02 细胞因子信号转导抑制因子3(SOCS3)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 3 (SOCS3)
PAH154Ra01 细胞因子信号转导抑制因子2(SOCS2)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 2 (SOCS2)
PAH154Hu01 细胞因子信号转导抑制因子2(SOCS2)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 2 (SOCS2)
PAH158Hu01 细胞因子信号转导抑制因子1(SOCS1)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 1 (SOCS1)
PAH158Ra01 细胞因子信号转导抑制因子1(SOCS1)多克隆抗体 Polyclonal Antibody to Suppressors Of Cytokine Signaling 1 (SOCS1)
PAM329Hu01 Zeste 12同源物1抑制因子2(SUZ12)多克隆抗体 Polyclonal Antibody to Suppressor Of Zeste 12 Homolog (SUZ12)
PAM970Mu01 彩斑4-20同源物1抑制因子2(SUV420H2)多克隆抗体 Polyclonal Antibody to Suppressor Of Variegation 4-20 Homolog 2 (SUV420H2)
PAM325Hu01 Ty 6同源物抑制因子(SUPT6H)多克隆抗体 Polyclonal Antibody to Suppressor Of Ty 6 Homolog (SUPT6H)
PAP657Hu01 瘤变抑制因子14(ST14)多克隆抗体 Polyclonal Antibody to Suppression Of Tumorigenicity 14 (ST14)
PAC881Hu01 监理蛋白(SVIL)多克隆抗体 Polyclonal Antibody to Supervillin (SVIL)
PAC881Hu02 监理蛋白(SVIL)多克隆抗体 Polyclonal Antibody to Supervillin (SVIL)
PAS134Hu01 超氧化物歧化酶(SOD)多克隆抗体 Polyclonal Antibody to Superoxide Dismutases (SOD)
PAS134Po01 超氧化物歧化酶(SOD)多克隆抗体 Polyclonal Antibody to Superoxide Dismutases (SOD)
PAA596Hu01 铜伴侣超氧化物歧化酶(SOD4)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase, Copper Chaperone (SOD4)
PAA596Mu01 铜伴侣超氧化物歧化酶(SOD4)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase, Copper Chaperone (SOD4)
PAA596Ra01 铜伴侣超氧化物歧化酶(SOD4)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase, Copper Chaperone (SOD4)
PAA117Hu01 胞外超氧化物歧化酶(SOD3)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 3, Extracellular (SOD3)
PAA117Mu01 胞外超氧化物歧化酶(SOD3)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 3, Extracellular (SOD3)
PAA117Ra01 胞外超氧化物歧化酶(SOD3)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 3, Extracellular (SOD3)
PAA117Rb51 胞外超氧化物歧化酶(SOD3)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 3, Extracellular (SOD3)
PAA117Mu02 胞外超氧化物歧化酶(SOD3)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 3, Extracellular (SOD3)
PAB083Mu01 线粒体超氧化物歧化酶(SOD2)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2)
PAB083Ra01 线粒体超氧化物歧化酶(SOD2)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2)
PAB083Hu01 线粒体超氧化物歧化酶(SOD2)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 2, Mitochondrial (SOD2)
PAB960Hu01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Mu01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Ra01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Bo01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Rb51 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Mu02 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Eq01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Ga01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Ov01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAB960Po01 超氧化物歧化酶1(SOD1)多克隆抗体 Polyclonal Antibody to Superoxide Dismutase 1, Soluble (SOD1)
PAK523Ge01 日落黄(SY)多克隆抗体 Polyclonal Antibody to Sunset Yellow (SY)
PAH097Hu01 亚硫酸盐氧化酶(SUOX)多克隆抗体 Polyclonal Antibody to Sulfite Oxidase (SUOX)
PAH097Mu01 亚硫酸盐氧化酶(SUOX)多克隆抗体 Polyclonal Antibody to Sulfite Oxidase (SUOX)
PAH097Ra01 亚硫酸盐氧化酶(SUOX)多克隆抗体 Polyclonal Antibody to Sulfite Oxidase (SUOX)
PAH097Ra02 亚硫酸盐氧化酶(SUOX)多克隆抗体 Polyclonal Antibody to Sulfite Oxidase (SUOX)
PAH097Mu02 亚硫酸盐氧化酶(SUOX)多克隆抗体 Polyclonal Antibody to Sulfite Oxidase (SUOX)
PAH097Bo01 亚硫酸盐氧化酶(SUOX)多克隆抗体 Polyclonal Antibody to Sulfite Oxidase (SUOX)
PAC879Hu01 硫酸酯酶修饰因子1(SUMF1)多克隆抗体 Polyclonal Antibody to Sulfatase Modifying Factor 1 (SUMF1)
PAH107Hu01 硫酸酯酶2(SULF2)多克隆抗体 Polyclonal Antibody to Sulfatase 2 (SULF2)
PAH107Mu01 硫酸酯酶2(SULF2)多克隆抗体 Polyclonal Antibody to Sulfatase 2 (SULF2)
PAH107Mu02 硫酸酯酶2(SULF2)多克隆抗体 Polyclonal Antibody to Sulfatase 2 (SULF2)
PAH108Hu01 硫酸酯酶1(SULF1)多克隆抗体 Polyclonal Antibody to Sulfatase 1 (SULF1)
PAD186Hu01 蔗糖酶异麦芽糖酶(SI)多克隆抗体 Polyclonal Antibody to Sucrase Isomaltase (SI)
PAK214Mu01 琥珀酸脱氢酶复合体D亚基(SDHD)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit D (SDHD)
PAK214Mu02 琥珀酸脱氢酶复合体D亚基(SDHD)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit D (SDHD)
PAK213Mu01 琥珀酸脱氢酶复合体C亚基(SDHC)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit C (SDHC)
PAK213Mu02 琥珀酸脱氢酶复合体C亚基(SDHC)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit C (SDHC)
PAJ756Mu01 琥珀酸脱氢酶复合体B亚基(SDHB)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase 可溶性苹果酸脱氢酶抗体Complex Subunit B (SDHB)
PAJ756Mu02 琥珀酸脱氢酶复合体B亚基(SDHB)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit B (SDHB)
PAJ784Mu01 琥珀酸脱氢酶复合体A亚基(SDHA)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit A (SDHA)
PAJ784Mu02 琥珀酸脱氢酶复合体A亚基(SDHA)多克隆抗体 Polyclonal Antibody to Succinate Dehydrogenase Complex Subunit A (SDHA)