间质细胞蛋白抗体

间质细胞蛋白抗体 

『别名』Malignant mesothelioma susceptibility; MMS. 

『浓度』间质细胞蛋白抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

间质细胞蛋白抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

间质细胞蛋白抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAG631Hu01 耐扭蛋白1A(TOR1A)多克隆抗体 Polyclonal Antibody to Torsin 1A (TOR1A)

PAG631Mu01 耐扭蛋白1A(TOR1A)多克隆抗体 Polyclonal Antibody to Torsin 1A (TOR1A)

PAD800Hu01 拓扑异构酶Ⅲ(TOP3)多克隆抗体 Polyclonal Antibody to Topoisomerase III (TOP3)

PAD798Hu01 拓扑异构酶Ⅱβ(TOP2β)多克隆抗体 Polyclonal Antibody to Topoisomerase II Beta (TOP2b)

PAD798Mu01 拓扑异构酶Ⅱβ(TOP2β)多克隆抗体 Polyclonal Antibody to Topoisomerase II Beta (TOP2b)

PAA792Hu01 拓扑异构酶Ⅱ(TOP2)多克隆抗体 Polyclonal Antibody to Topoisomerase II (TOP2)

PAA792Mu01 拓扑异构酶Ⅱ(TOP2)多克隆抗体 Polyclonal Antibody to Topoisomerase II (TOP2)

PAA792Mu02 拓扑异构酶Ⅱ(TOP2)多克隆抗体 Polyclonal Antibody to Topoisomerase II (TOP2)

PAA792Ra01 拓扑异构酶Ⅱ(TOP2)多克隆抗体 Polyclonal Antibody to Topoisomerase II (TOP2)

PAC243Hu01 拓扑异构酶Ⅰ(TOP1)多克隆抗体 Polyclonal Antibody to Topoisomerase I (TOP1)

PAC243Mu01 拓扑异构酶Ⅰ(TOP1)多克隆抗体 Polyclonal Antibody to Topoisomerase I (TOP1)

PAC243Mu02 拓扑异构酶Ⅰ(TOP1)多克隆抗体 Polyclonal Antibody to Topoisomerase I (TOP1)

PAM377Hu01 肿瘤调节蛋白1(TR1)多克隆抗体 Polyclonal Antibody to Tomoregulin 1 (TR1)

PAM377Mu01 肿瘤调节蛋白1(TR1)多克隆抗体 Polyclonal Antibody to Tomoregulin 1 (TR1)

PAM378Hu01 肿瘤调节蛋白(TR)多克隆抗体 Polyclonal Antibody to Tomoregulin (TR)

PAM373Hu01 Tolloid样蛋白1(TLL1)多克隆抗体 Polyclonal Antibody to Tolloid Like Protein 1 (TLL1)

PAH021Hu01 Toll样受体衔接分子2(TICAM2)多克隆抗体 Polyclonal Antibody to Toll Like Receptor Adaptor Molecule 2 (TICAM2)

PAH021Mu01 Toll样受体衔接分子2(TICAM2)多克隆抗体 Polyclonal Antibody to Toll Like Receptor Adaptor Molecule 2 (TICAM2)

PAH022Hu01 Toll样受体衔接分子1(TICAM1)多克隆抗体 Polyclonal Antibody to Toll Like Receptor Adaptor Molecule 1 (TICAM1)

PAH022Mu01 Toll样受体衔接分子1(TICAM1)多克隆抗体 Polyclonal Antibody to Toll Like Receptor Adaptor Molecule 1 (TICAM1)

PAH022Mu02 Toll样受体衔接分子1(TICAM1)多克隆抗体 Polyclonal Antibody to Toll Like Receptor Adaptor Molecule 1 (TICAM1)

PAA709Hu01 Toll样受体9(TLR9)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 9 (TLR9)

PAB991Hu01 Toll样受体8(TLR8)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 8 (TLR8)

PAB950Hu01 Toll样受体7(TLR7)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 7 (TLR7)

PAB950Mu01 Toll样受体7(TLR7)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 7 (TLR7)

PAB950Mu02 Toll样受体7(TLR7)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 7 (TLR7)

PAA683Hu01 Toll样受体6(TLR6)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 6 (TLR6)

PAA683Mu01 Toll样受体6(TLR6)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 6 (TLR6)

PAA683Mu02 Toll样受体6(TLR6)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 6 (TLR6)

PAB990Hu01 Toll样受体5(TLR5)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 5 (TLR5)

PAB990Hu03 Toll样受体5(TLR5)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 5 (TLR5)

PAB990Hu02 Toll样受体5(TLR5)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 5 (TLR5)

PAA753Bo01 Toll样受体4(TLR4)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 4 (TLR4)

PAA753Hu01 Toll样受体4(TLR4)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 4 (TLR4)

PAA753Mu01 Toll样受体4(TLR4)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 4 (TLR4)

PAA753Ra01 Toll样受体4(TLR4)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 4 (TLR4)

PAA753Mu02 Toll样受体4(TLR4)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 4 (TLR4)

PAB989Hu01 Toll样受体3(TLR3)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 3 (TLR3)

PAB989Mu01 Toll样受体3(TLR3)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 3 (TLR3)

PAB989Mu02 Toll样受体3(TLR3)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 3 (TLR3)

PAB989Mu03 Toll样受体3(TLR3)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 3 (TLR3)

PAA663Hu01 Toll样受体2(TLR2)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 2 (TLR2)

PAA663Mu01 Toll样受体2(TLR2)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 2 (TLR2)

PAA663Ra01 Toll样受体2(TLR2)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 2 (TLR2)

PAA663Hu02 Toll样受体2(TLR2)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 2 (TLR2)

PAA663Mu02 Toll样受体2(TLR2)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 2 (TLR2)

PAB992Hu01 Toll样受体10(TLR10)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 10 (TLR10)

PAB992Ra01 Toll样受体10(TLR10)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 10 (TLR10)

PAB988Hu01 Toll样受体1(TLR1)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 1 (TLR1)

PAB988Hu02 Toll样受体1(TLR1)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 1 (TLR1)

PAB988Mu01 Toll样受体1间质细胞蛋白抗体(TLR1)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 1 (TLR1)

PAB988Mu02 Toll样受体1(TLR1)多克隆抗体 Polyclonal Antibody to Toll Like Receptor 1 (TLR1)

PAN888Hu01 含Toll白介素1受体域衔接蛋白(TIRAP)多克隆抗体 Polyclonal Antibody to Toll Interleukin 1 Receptor Domain Containing Adaptor Protein (TIRAP)

PAC803Hu01 Toll相互作用蛋白(TOLLIP)多克隆抗体 Polyclonal Antibody to Toll Interacting Protein (TOLLIP)

PAC803Mu01 Toll相互作用蛋白(TOLLIP)多克隆抗体 Polyclonal Antibody to Toll Interacting Protein (TOLLIP)