跨膜结构域4亚家族成员2抗体

?跨膜结构域4亚家族成员2抗体 

『别名』APY; ATOPY; Fc IgE receptor beta chain; FCER1B; FCERI; IGEL; IGHER; MS4A1; High affinity immunoglobulin epsilon receptor subunit beta; FcERI; Fc epsilon receptor I beta-chain; IgE Fc receptor subunit beta; Membrane-spanning 4-domains subfamily A member 2; Fc epsilon receptor I beta-chain; membrane-spanning 4-domains, subfamily A, member 2; Fc fragment of IgE, high affinity I, receptor for; beta polypeptide; FCERB_RAT. 

『浓度』跨膜结构域4亚家族成员2抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

跨膜结构域4亚家族成员2抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

跨膜结构域4亚家族成员2抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAA397Mu02 转化生长因子β受体Ⅰ(TGFβR1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Receptor I (TGFbR1)

PAA131Bo01 转化生长因子β诱导蛋白(TGFβI)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Induced Protein (TGFbI)

PAA131Fi01 转化生长因子β诱导蛋白(TGFβI)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Induced Protein (TGFbI)

PAA131Hu01 转化生长因子β诱导蛋白(TGFβI)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Induced Protein (TGFbI)

PAA131Mu01 转化生长因子β诱导蛋白(TGFβI)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Induced Protein (TGFbI)

PAA131Po01 转化生长因子β诱导蛋白(TGFβI)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Induced Protein (TGFbI)

PAA131Rb51 转化生长因子β诱导蛋白(TGFβI)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Induced Protein (TGFbI)

PAA131Mu02 转化生长因子β诱导蛋白(TGFβI)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Induced Protein (TGFbI)

PAB949Hu01 转化生长因子β3(TGFβ3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3)

PAB949Mu01 转化生长因子β3(TGFβ3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3)

PAB949Ra01 转化生长因子β3(TGFβ3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3)

PAB949Po01 转化生长因子β3(TGFβ3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3)

PAB949Mu02 转化生长因子β3(TGFβ3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3)

PAB949Hu02 转化生长因子β3(TGFβ3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3)

PAB949Bo01 转化生长因子β3(TGFβ3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 3 (TGFb3)

PAA218Hu01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Mu01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Ra01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Bo01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Ca01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Cp01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Ga01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Po01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Rb51 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Si01 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAA218Mu02 转化生长因子β2(TGFβ2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 2 (TGFb2)

PAH039Hu01 转化生长因子β1诱导转录因子1(TGFβ1I1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1)

PAH039Ra01 转化生长因子β1诱导转录因子1(TGFβ1I1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 Induced Transcript 1 (TGFb1I1)

PAA124Hu01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Mu01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Ra01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Gu01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Rb51 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Ca01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Bo01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Po01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Cp01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Eq01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Ga01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Fi01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Si01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Mu02 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA124Ov01 转化生长因子β1(TGFβ1)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta 1 (TGFb1)

PAA123Hu01 转化生长因子α(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAA123Mu01 转化生长因子α(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAA123Ra01 转化生长因子α(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAA123Cp01 转化生长因子α(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAA123Po01 转化生长因子α(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAA123Rb51 转化生长因子α(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAA123Si01 转化生长因子α跨膜结构域4亚家族成员2抗体(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAA123Mu02 转化生长因子α(TGFα)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Alpha (TGFa)

PAM389Hu01 转化因子2β(TRA2β)多克隆抗体 Polyclonal Antibody to Transformer 2 Beta (TRA2b)

PAG996Hu01 转化/转录域关联蛋白(TRRAP)多克隆抗体 Polyclonal Antibody to Transformation/Transcription Domain Associated Protein (TRRAP)