组织相容性复合体蛋白2抗体
『别名』DMA; DMB; DRB; HLA class II histocompatibility antigen DM beta chain; HLA DMB; HLADM; HLADP1B; Major histocompatibility complex clas; HLA class II histocompatibility antigen, DM beta chain; MHC class II antigen DMB; Really interesting new gene 7 protein; HLA-DMB; RING7; DMB_HUMAN.
『浓度』组织相容性复合体蛋白2抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
组织相容性复合体蛋白2抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
组织相容性复合体蛋白2抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA272Bo01 转运蛋白1(TNPO1)多克隆抗体 Polyclonal Antibody to Transportin 1 (TNPO1)
PAA272Hu01 转运蛋白1(TNPO1)多克隆抗体 Polyclonal Antibody to Transportin 1 (TNPO1)
PAA272Mu01 转运蛋白1(TNPO1)多克隆抗体 Polyclonal Antibody to Transportin 1 (TNPO1)
PAK477Hu01 跨膜蛋白27(TMEM27)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 27 (TMEM27)
PAK477Mu01 跨膜蛋白27(TMEM27)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 27 (TMEM27)
PAK477Ra01 跨膜蛋白27(TMEM27)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 27 (TMEM27)
PAK477Ca01 跨膜蛋白27(TMEM27)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 27 (TMEM27)
PAK477Mu02 跨膜蛋白27(TMEM27)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 27 (TMEM27)
PAR965Hu01 跨膜蛋白2样蛋白(TMEM2L)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 2 Like Protein (TMEM2L)
PAN011Hu01 跨膜蛋白173(TMEM173)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 173 (TMEM173)
PAN011Ra01 跨膜蛋白173(TMEM173)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 173 (TMEM173)
PAN011Mu01 跨膜蛋白173(TMEM173)多克隆抗体 Polyclonal Antibody to Transmembrane Protein 173 (TMEM173)
PAF817Hu01 跨膜丝氨酸蛋白酶6(TMPRSS6)多克隆抗体 Polyclonal Antibody to Transmembrane Protease, Serine 6 (TMPRSS6)
PAF800Hu01 跨膜丝氨酸蛋白酶4(TMPRSS4)多克隆抗体 Polyclonal Antibody to Transmembrane Protease, Serine 4 (TMPRSS4)
PAC795Hu01 跨膜丝氨酸蛋白酶2(TMPRSS2)多克隆抗体 Polyclonal Antibody to Transmembrane Protease, Serine 2 (TMPRSS2)
PAC795Mu01 跨膜丝氨酸蛋白酶2(TMPRSS2)多克隆抗体 Polyclonal Antibody to Transmembrane Protease, Serine 2 (TMPRSS2)
PAC795Mu02 跨膜丝氨酸蛋白酶2(TMPRSS2)多克隆抗体 Polyclonal Antibody to Transmembrane Protease, Serine 2 (TMPRSS2)
PAJ628Hu01 转位因子蛋白(TSPO)多克隆抗体 Polyclonal Antibody to Translocator Protein (TSPO)
PAF824Hu01 易位关联膜蛋白1(TRAM1)多克隆抗体 Polyclonal Antibody to Translocation Associated Membrane Protein 1 (TRAM1)
PAP574Hu01 线粒体外膜转位酶70A(TOMM70A)多克隆抗体 Polyclonal Antibody to Translocase Of Outer Mitochondrial Membrane 70A (TOMM70A)
PAH018Hu01 转酮醇酶样蛋白1(TKTL1)多克隆抗体 Polyclonal Antibody to Transketolase Like Protein 1 (TKTL1)
PAH019Hu01 转酮醇酶(TKT)多克隆抗体 Polyclonal Antibody to Transketolase (TKT)
PAH019Ra01 转酮醇酶(TKT)多克隆抗体 Polyclonal Antibody to Transketolase (TKT)
PAA331Mu01 转换蛋白1(TNP1)多克隆抗体 Polyclonal Antibody to Transition Protein 1 (TNP1)
PAF848Hu01 转化受体电位阳离子通道亚家族V成员3(TRPV3)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily V, Member 3 (TRPV3)
PAF840Mu01 转化受体电位阳离子通道亚家族V成员2(TRPV2)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily V, Member 2 (TRPV2)
PAF840Mu02 转化受体电位阳离子通道亚家族V成员2(TRPV2)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily V, Member 2 (TRPV2)
PAF839Hu01 转化受体电位阳离子通道亚家族V成员1(TRPV1)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)
PAF839Mu01 转化受体电位阳离子通道亚家族V成员1(TRPV1)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)
PAF839Mu02 转化受体电位阳离子通道亚家族V成员1(TRPV1)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily V, Member 1 (TRPV1)
PAF838Hu01 转化受体电位阳离子通道亚家族M成员8(TRPM8)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8)
PAF837Ra01 转化受体电位阳离子通道亚家族M成员7(TRPM7)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7)
PAF836Ra01 转化受体电位阳离子通道亚家族M成员6(TRPM6)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily M, Member 6 (TRPM6)
PAF834Mu01 转化受体电位阳离子通道亚家族M成员4(TRPM4)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4)
PAF834Ra01 转化受体电位阳离子通道亚家族M成员4(TRPM4)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4)
PAF834Mu02 转化受体电位阳离子通道亚家族M成员4(TRPM4)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4)
PAC812Hu01 转化受体电位阳离子通道亚家族M成员1(TRPM1)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily M, Member 1 (TRPM1)
PAF846Hu01 转化受体电位阳离子通道亚家族C成员6(TRPC6)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily C, Member 6 (TRPC6)
PAF831Hu01 转化受体电位阳离子通道亚家族A成员1(TRPA1)多克隆抗体 Polyclonal Antibody to Transient Receptor Potential Cation Channel Subfamily A, Member 1 (TRPA1)
PAH033Hu01 转谷氨酰胺酶6(TGM6)多克隆抗体 Polyclonal Antibody to Transglutaminase 6 (TGM6)
PAH033Mu01 转谷氨酰胺酶6(TGM6)多克隆抗体 Polyclonal Antibody to Transglutaminase 6 (TGM6)
PAH031Hu01 前列腺转谷氨酰胺酶(TGM4)多克隆抗体 Polyclonal Antibody to Transglutaminase 4, Prostate (TGM4)
PAB756Hu01 表皮转谷氨酰胺酶(TGM3)多克隆抗体 Polyclonal Antibody to Transglutaminase 3, Epidermal (TGM3)
PAB756Mu01 表皮转谷氨酰胺酶(TGM3)多克隆抗体 Polyclonal Antibody to Transglutaminase 3, Epidermal (TGM3)
PAB756Mu02 表皮转谷氨酰胺酶(TGM3)多克隆抗体 Polyclonal Antibody to Transglutaminase 3, Epidermal (TGM3)
PAB830Hu01 组织转谷氨酰胺酶(TGM2)多克隆抗体 Polyclonal Antibody to Transglutaminase 2, Tissue (TGM2)
PAB830Ra01 组织转谷氨酰胺酶(TGM2)多克隆抗体 Polyclonal Antibody to Transglutaminase 2, Tissue (TGM2)
PAB773Hu01 角质转谷氨酰胺酶(TGM1)多克隆抗体 Polyclonal Antibody to Transglutaminase 1, Keratinocyte (TGM1)
PAB773Ra01 角质转谷氨酰胺酶(TGM1)多克隆抗体 Polyclonal Antibody to Transglutaminase 1, Keratinocyte (TGM1)
PAB773Ca01 角质转谷氨酰胺酶(TGM1)多克隆抗体 Polyclonal Antibody to Transglutaminase 1, Keratinocyte (TGM1)
PAB773Mu01 角质转谷氨酰胺酶(TGM1)多克隆抗体 Polyclonal Antibody to Transglutaminase 1, Keratinocyte (TGM1)
PAB773Rb51 角质转谷氨酰胺酶(TGM1)多克隆抗体 Polyclonal Antibody to Transglutaminase 1, Keratinocyte (TGM1)
PAB773Mu02 角质转谷氨酰胺酶(TGM1)多克隆抗体 Polyclonal Antibody to Transglutaminase 1, Keratinocyte (TGM1)
PAF790Hu01 转胶蛋白2(TAGLN2)多克隆抗体 Polyclonal Antibody to Transgelin 2 (TAGLN2)
PAC781Hu01 转胶蛋白(TAGLN)多克隆抗体 Polyclonal Antibody to Transgelin (TAGLN)
PAC781Ra01 转胶蛋白组织相容性复合体蛋白2抗体(TAGLN)多克隆抗体 Polyclonal Antibody to Transgelin (TAGLN)
PAB637Hu01 转化生长因子β刺激蛋白22(TSC22)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Stimulated Protein Clone 22 (TSC22)
PAC119Ra01 转化生长因子β受体Ⅲ(TGFβR3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Receptor III (TGFbR3)
PAC119Mu01 转化生长因子β受体Ⅲ(TGFβR3)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Receptor III (TGFbR3)
PAC972Hu01 转化生长因子β受体Ⅱ(TGFβR2)多克隆抗体 Polyclonal Antibody to Transforming Growth Factor Beta Receptor II (TGFbR2)