组织相容性复合体蛋白1抗体
『别名』MHC-1; MHC1; MHC 1; A 28; A 9; Aw 24; Aw 68; CLASS I HISTOCOMPATIBILITY ANTIGEN; H2 K1; H2K; HLA A; HLA class I histocompatibility antigen A 1 alpha chain; HLA class I histocompatibility antigen A 11 alpha chain; HLA class I histocompatibility antigen A 2 alpha chain; HLA class I histocompatibility antigen A 24 alpha chain; HLA class I histocompatibility antigen A 3 alpha chain; HLA class I histocompatibility antigen A 30 alpha chain; HLA class I histocompatibility antigen A 32 alpha chain; HLA class I histocompatibility antigen A 68 alpha chain; HLAA; Major histocompatibility complex class I A; MHC class I heavy chain H2 K.
『浓度』组织相容性复合体蛋白1抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
组织相容性复合体蛋白1抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
组织相容性复合体蛋白1抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAB339Hu01 肌钙蛋白C(TNC)多克隆抗体 Polyclonal Antibody to Troponin C (TNC)
PAB339Ra01 肌钙蛋白C(TNC)多克隆抗体 Polyclonal Antibody to Troponin C (TNC)
PAB339Mu01 肌钙蛋白C(TNC)多克隆抗体 Polyclonal Antibody to Troponin C (TNC)
PAB339Rb51 肌钙蛋白C(TNC)多克隆抗体 Polyclonal Antibody to Troponin C (TNC)
PAD449Hu01 原肌球蛋白3(TPM3)多克隆抗体 Polyclonal Antibody to Tropomyosin 3 (TPM3)
PAD449Mu01 原肌球蛋白3(TPM3)多克隆抗体 Polyclonal Antibody to Tropomyosin 3 (TPM3)
PAD448Ra01 原肌球蛋白2β(TPM2)多克隆抗体 Polyclonal Antibody to Tropomyosin 2 Beta (TPM2)
PAG687Mu01 原肌球调节蛋白3(TMOD3)多克隆抗体 Polyclonal Antibody to Tropomodulin 3 (TMOD3)
PAG687Mu02 原肌球调节蛋白3(TMOD3)多克隆抗体 Polyclonal Antibody to Tropomodulin 3 (TMOD3)
PAG689Hu01 原肌球调节蛋白1(TMOD1)多克隆抗体 Polyclonal Antibody to Tropomodulin 1 (TMOD1)
PAA434Hu01 滋养层特异性糖蛋白(TPBG)多克隆抗体 Polyclonal Antibody to Trophoblast Glycoprotein (TPBG)
PAA434Mu01 滋养层特异性糖蛋白(TPBG)多克隆抗体 Polyclonal Antibody to Trophoblast Glycoprotein (TPBG)
PAC828Hu01 三肽基肽酶Ⅰ(TPP1)多克隆抗体 Polyclonal Antibody to Tripeptidyl Peptidase I (TPP1)
PAC828Mu01 三肽基肽酶Ⅰ(TPP1)多克隆抗体 Polyclonal Antibody to Tripeptidyl Peptidase I (TPP1)
PAC828Mu02 三肽基肽酶Ⅰ(TPP1)多克隆抗体 Polyclonal Antibody to Tripeptidyl Peptidase I (TPP1)
PAH006Hu01 丙糖磷酸异构酶1(TPI1)多克隆抗体 Polyclonal Antibody to Triosephosphate Isomerase 1 (TPI1)
PAH006Mu01 丙糖磷酸异构酶1(TPI1)多克隆抗体 Polyclonal Antibody to Triosephosphate Isomerase 1 (TPI1)
PAH006Mu02 丙糖磷酸异构酶1(TPI1)多克隆抗体 Polyclonal Antibody to Triosephosphate Isomerase 1 (TPI1)
PAA453Ge01 三碘甲状腺原氨酸(T3)多克隆抗体 Polyclonal Antibody to Triiodothyronine (T3)
PAG628Hu01 髓细胞触发受体2(TREM2)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)
PAG628Mu01 髓细胞触发受体2(TREM2)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)
PAG628Mu02 髓细胞触发受体2(TREM2)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)
PAG628Ra01 髓细胞触发受体2(TREM2)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 2 (TREM2)
PAA213Bo01 髓细胞触发受体1(TREM1)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)
PAA213Hu01 髓细胞触发受体1(TREM1)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)
PAA213Mu01 髓细胞触发受体1(TREM1)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)
PAA213Po01 髓细胞触发受体1(TREM1)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)
PAA213Ra01 髓细胞触发受体1(TREM1)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)
PAA213Mu02 髓细胞触发受体1(TREM1)多克隆抗体 Polyclonal Antibody to Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)
PAG480Hu01 毛透明蛋白(TCHH)多克隆抗体 Polyclonal Antibody to Trichohyalin (TCHH)
PAG871Mu01 海藻糖酶(TREH)多克隆抗体 Polyclonal Antibody to Trehalase (TREH)
PAG871Mu02 海藻糖酶(TREH)多克隆抗体 Polyclonal Antibody to Trehalase (TREH)
PAG871Hu01 海藻糖酶(TREH)多克隆抗体 Polyclonal Antibody to Trehalase (TREH)
PAB656Hu01 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Mu01 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Ra01 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Po01 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Ca01 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Mu02 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Po02 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Po03 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAB656Bo01 三叶因子3(TFF3)多克隆抗体 Polyclonal Antibody to Trefoil Factor 3, Intestinal (TFF3)
PAA748Hu01 三叶因子2(TFF2)多克隆抗体 Polyclonal Antibody to Trefoil Factor 2 (TFF2)
PAA748Mu01 三叶因子2(TFF2)多克隆抗体 Polyclonal Antibody to Trefoil Factor 2 (TFF2)
PAA748Ra01 三叶因子2(TFF2)多克隆抗体 Polyclonal Antibody to Trefoil Factor 2 (TFF2)
PAA748Mu02 三叶因子2(TFF2)多克隆抗体 Polyclonal Antibody to Trefoil Factor 2 (TFF2)
PAB049Hu01 三叶因子1(TFF1)多克隆抗体 Polyclonal Antibody to Trefoil Factor 1 (TFF1)
PAB049Ra01 三叶因子1(TFF1)多克隆抗体 Polyclonal Antibody to Trefoil Factor 1 (TFF1)
PAB049Mu01 三叶因子1(TFF1)多克隆抗体 Polyclonal Antibody to Trefoil Factor 1 (TFF1)
PAA726Hu01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Mu01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Ra01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Bo01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Po01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Ga01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Hu02 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Mu02 转甲状腺素蛋白组织相容性复合体蛋白1抗体(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Ca01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Gu01 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)
PAA726Rb51 转甲状腺素蛋白(TTR)多克隆抗体 Polyclonal Antibody to Transthyretin (TTR)