组蛋白乙酰转移酶MOF抗体
『别名』EC 2.3.1.48; Histone acetyltransferase KAT8; Histone acetyltransferase MYST1; hMOF; K(lysine) acetyltransferase 8; KAT 8; Lysine acetyltransferase 8; MOF; MOZ; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1; MYST 1; MYST histone acetyltransferase 1; myst protein 1; MYST-1; MYST1; MYST1_HUMAN; Ortholog of Drosophila males absent on the first (MOF); Probable histone acetyltransferase MYST1; SAS2 and TIP60 protein 1; SAS2; SAS3; TIP60 protein 1; YBF2; YBF2/SAS3.
『浓度』组蛋白乙酰转移酶MOF抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
组蛋白乙酰转移酶MOF抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
组蛋白乙酰转移酶MOF抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAB750Ra01 肿瘤坏死因子配体超家族成员13(TNFSF13)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)
PAB750Si01 肿瘤坏死因子配体超家族成员13(TNFSF13)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)
PAB750Mu02 肿瘤坏死因子配体超家族成员13(TNFSF13)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)
PAB750Bo01 肿瘤坏死因子配体超家族成员13(TNFSF13)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 13 (TNFSF13)
PAD933Ra01 肿瘤坏死因子配体超家族成员12(TNFSF12)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12)
PAD933Hu01 肿瘤坏死因子配体超家族成员12(TNFSF12)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12)
PAD933Hu02 肿瘤坏死因子配体超家族成员12(TNFSF12)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12)
PAD933Mu01 肿瘤坏死因子配体超家族成员12(TNFSF12)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Ligand Superfamily, Member 12 (TNFSF12)
PAA134Bo01 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Ca01 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Hu01 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Mu01 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Po01 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Ra01 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Rb51 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Si01 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAA134Mu02 肿瘤坏死因子β(TNFβ)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Beta (TNFb)
PAM405Mu01 肿瘤坏死因子α诱导蛋白6(TNFαIP6)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6)
PAM405Mu02 肿瘤坏死因子α诱导蛋白6(TNFαIP6)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 6 (TNFaIP6)
PAF805Mu01 肿瘤坏死因子α诱导蛋白3相互作用蛋白2(TNIP2)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 3 Interacting Protein 2 (TNIP2)
PAF805Ra01 肿瘤坏死因子α诱导蛋白3相互作用蛋白2(TNIP2)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 3 Interacting Protein 2 (TNIP2)
PAF805Hu01 肿瘤坏死因子α诱导蛋白3相互作用蛋白2(TNIP2)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 3 Interacting Protein 2 (TNIP2)
PAC796Hu01 肿瘤坏死因子α诱导蛋白3(TNFαIP3)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 3 (TNFaIP3)
PAF802Hu01 肿瘤坏死因子α诱导蛋白2(TNFαIP2)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha Induced Protein 2 (TNFaIP2)
PAA133Hu01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Mu01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Ra01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Gu01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Rb52 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Si01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Ca01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Po01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Bo01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Cp01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Eq01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Ov01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Rb51 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Mu02 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAA133Ga01 肿瘤坏死因子α(TNFα)多克隆抗体 Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)
PAG483Hu01 间质性肾炎抗原样蛋白1(TINAGL1)多克隆抗体 Polyclonal Antibody to Tubulointerstitial Nephritis Antigen Like Protein 1 (TINAGL1)
PAA993Mu01 促微管蛋白聚合蛋白(TPPP)多克隆抗体 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP)
PAA993Hu01 促微管蛋白聚合蛋白(TPPP)多克隆抗体 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP)
PAA993Mu02 促微管蛋白聚合蛋白(TPPP)多克隆抗体 Polyclonal Antibody to Tubulin Polymerization Promoting Protein (TPPP)
PAE719Mu01 微管蛋白ε(TUBε)多克隆抗体 Polyclonal Antibody to Tubulin Epsilon (TUBe)
PAE719Hu01 微管蛋白ε(TUBε)多克隆抗体 Polyclonal Antibody to Tubulin Epsilon (TUBe)
PAE718Mu01 微管蛋白δ(TUBδ)多克隆抗体 Polyclonal Antibody to Tubulin Delta (TUBd)
PAE718Ra01 微管蛋白δ(TUBδ)多克隆抗体 Polyclonal Antibody to Tubulin Delta (TUBd)
PAE718Hu01 微管蛋白δ(TUBδ)多克隆抗体 Polyclonal Antibody to Tubulin Delta (TUBd)
PAE712Mu01 微管蛋白β6(TUBβ6)多克隆抗体 Polyclonal Antibody to Tubulin Beta 6 (TUBb6)
PAE711Hu01 微管蛋白β3(TUBβ3)多克隆抗体 Polyclonal Antibody to Tubulin Beta 3 (TUBb3)
PAE707Mu01 微管蛋白β1(TUBβ1)多克隆抗体 Polyclonal Antibody to Tubulin Beta 1 (TUBb1)
PAE707Mu02 微管蛋白β1(TUBβ1)多克隆抗体 Polyclonal Antibody to Tubulin Beta 1 (TUBb1)
PAE707Hu01 微管蛋白β1组蛋白乙酰转移酶MOF抗体(TUBβ1)多克隆抗体 Polyclonal Antibody to Tubulin Beta 1 (TUBb1)
PAB870Hu01 微管蛋白β(TUBβ)多克隆抗体 Polyclonal Antibody to Tubulin Beta (TUBb)
PAE716Mu01 微管蛋白α3A(TUBα3A)多克隆抗体 Polyclonal Antibody to Tubulin Alpha 4A (TUBa4A)
PAE716Mu02 微管蛋白α3A(TUBα3A)多克隆抗体 Polyclonal Antibody to Tubulin Alpha 4A (TUBa4A)