线粒体转录终止因子1抗体

线粒体转录终止因子1抗体 

『别名』mitochondrial; Mitochondrial transcription termination factor 1; MTERF1; mTERF; MTERF_HUMAN; Transcription termination factor. 

『浓度』线粒体转录终止因子1抗体1mg/1ml 

『规格』0.2ml/200μg     

『抗体来源』Rabbit  

抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展，抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务，并将成为您在科研及生产中的得力助手。

我公司免费提供抗血清亲合层析纯化服务，得到目的蛋白的抗体IgG。

我公司还免费提供真空冷冻抽干服务，得到冻干粉状的抗体IgG产品，以便产品的使用及长期保存。

线粒体转录终止因子1抗体洗脱问题

抗体组目前采用的是低pH洗脱方法，按道理说，这个方法应该可以洗脱掉纯大部分抗体，但是有时候出现了一些顽固抗体很难洗脱，大家可以考虑其它的洗脱方法，beads说明书上提到了五大类办法，我觉得比较可靠的方法只有两类，一是改变pH，二是改变离子浓度，另外几种办法要么会带入有机试剂，要么会带入氨基，所以不太适用。改变pH的办法除了用低pH，还可以考虑用高pH来洗脱，好像没多少人尝试过这种办法，在确实没有办法的时候推荐尝试一下。 

线粒体转录终止因子1抗体其它问题

1、看了很多抗体纯化的资料，不管是从血清中纯化多抗还是从腹水中纯化单抗，都推荐大家在上柱子之前把血清或腹水稀释2-5倍，另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了，但是稀释这一步推荐大家试一试，有文献说稀释可以提高回收率

2、封闭这一步做完后，是不是考虑可以用HCl洗一次？连在beads上的蛋白有的是蛋白间非特异性结合上去的，洗脱之前的任何步骤也许都无法洗干净它们，所以这里把它们都洗掉，以免最后都洗到抗体里去了。

3、CNBr活化的琼脂糖或葡聚糖本身的缺陷：这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白，其中包括我们要的抗体，它们都带有一定的负电，所以都会和这个部位进行非特异性结合，在洗脱前，有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的，但是它也会洗掉直接与beads非特异结合的蛋白，也包括我们要的抗体，至于这一步会丢掉多少抗体，好像没有人测过。

PAG943Bo01 泛素羧基端酯酶L5(UCHL5)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5)

PAG943Hu01 泛素羧基端酯酶L5(UCHL5)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5)

PAS547Mu01 泛素羧基端酯酶L4(UCHL4)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L4 (UCHL4)

PAG944Hu01 泛素羧基端酯酶L3(UCHL3)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)

PAG944Ra01 泛素羧基端酯酶L3(UCHL3)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L3 (UCHL3)

PAG945Hu01 泛素羧基端酯酶L1(UCHL1)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PAG945Ra01 泛素羧基端酯酶L1(UCHL1)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PAG945Mu01 泛素羧基端酯酶L1(UCHL1)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PAG945Mu02 泛素羧基端酯酶L1(UCHL1)多克隆抗体 Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PAE593Hu01 泛素关联蛋白2(UBAP2)多克隆抗体 Polyclonal Antibody to Ubiquitin Associated Protein 2 (UBAP2)

PAB027Hu01 泛素激活酶样蛋白(UBE1L)多克隆抗体 Polyclonal Antibody to Ubiquitin Activating Enzyme E1 Like Protein (UBE1L)

PAB027Mu01 泛素激活酶样蛋白(UBE1L)多克隆抗体 Polyclonal Antibody to Ubiquitin Activating Enzyme E1 Like Protein (UBE1L)

PAB027Ra01 泛素激活酶样蛋白(UBE1L)多克隆抗体 Polyclonal Antibody to Ubiquitin Activating Enzyme E1 Like Protein (UBE1L)

PAE592Hu01 泛素A52残留核糖体蛋白融合产物1(UBA52)多克隆抗体 Polyclonal Antibody to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52)

PAA164Ga01 泛素(Ub)多克隆抗体 Polyclonal Antibody to Ubiquitin (Ub)

PAA164Gu01 泛素(Ub)多克隆抗体 Polyclonal Antibody to Ubiquitin (Ub)

PAA164Mi01 泛素(Ub)多克隆抗体 Polyclonal Antibody to Ubiquitin (Ub)

PAA164Mi02 泛素(Ub)多克隆抗体 Polyclonal Antibody to Ubiquitin (Ub)

PAG916Mu01 泛醌细胞色素C还原酶核心蛋白Ⅱ(UQCRC2)多克隆抗体 Polyclonal Antibody to Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2)

PAG916Mu02 泛醌细胞色素C还原酶核心蛋白Ⅱ(UQCRC2)多克隆抗体 Polyclonal Antibody to Ubiquinol Cytochrome C Reductase Core Protein II (UQCRC2)

PAG915Hu01 泛醇细胞色素C还原酶核心蛋白Ⅰ(UQCRC1)多克隆抗体 Polyclonal Antibody to Ubiquinol Cytochrome C Reductase Core Protein I (UQCRC1)

PAD311Hu01 泛醇细胞色素C还原酶(UQCR)多克隆抗体 Polyclonal Antibody to Ubiquinol Cytochrome C Reductase (UQCR)

PAG948Hu01 泛醌蛋白2(UBQLN2)多克隆抗体 Polyclonal Antibody to Ubiquilin 2 (UBQLN2)

PAD003Hu01 酪氨酰tRNA合成酶(YARS)多克隆抗体 Polyclonal Antibody to Tyrosyl tRNA Synthetase (YARS)

PAC797Hu01 非受体酪氨酸激酶2(TNK2)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase, Non Receptor 2 (TNK2)

PAA125Bo01 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

PAA125Fi01 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

PAA125Hu01 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

PAA125Mu01 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

PAA125Ra01 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

PAA125Mu02 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

PAA125Hu02 免疫球蛋白样EGF样域酪氨酸激酶1(Tie1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)

PAB976Hu01 酪氨酸激酶激活蛋白1(TKA1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase Activator Protein 1 (TKA1)

PAB976Hu02 酪氨酸激酶激活蛋白1(TKA1)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase Activator Protein 1 (TKA1)

PAB595Hu01 酪氨酸激酶2(Tyk2)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase 2 (Tyk2)

PAB595Mu01 酪氨酸激酶2(Tyk2)多克隆抗体 Polyclonal Antibody to Tyrosine Kinase 2 (Tyk2)

PAB438Hu01 酪氨酸羟化酶(TH)多克隆抗体 Polyclonal Antibody to Tyrosine Hydroxylase (TH)

PAB438Mu01 酪氨酸羟化酶(TH)多克隆抗体 Polyclonal Antibody to Tyrosine Hydroxylase (TH)

PAB438Ra01 酪氨酸羟化酶(TH)多克隆抗体 Polyclonal Antibody to Tyrosine Hydroxylase (TH)

PAB438Mu02 酪氨酸羟化酶(TH)多克隆抗体 Polyclonal Antibody to Tyrosine Hydroxylase (TH)

PAJ809Hu01 酪氨酸3/色氨酸5单加氧酶激活蛋白ζ(YWHAζ)多克隆抗体 Polyclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

PAJ809Ra01 酪氨酸3/色氨酸5单加氧酶激活蛋白ζ(YWHAζ)多克隆抗体 Polyclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz)

PAJ808Mi01 酪氨酸3/色氨酸5单加氧酶活化蛋白θ(YWHAθ)多克隆抗体 Polyclonal Antibody to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Theta (YWHAq)

PAS112Ge01 酪氨酸(Tyr)多克隆抗体 Polyclonal Antibody to Tyrosine (Tyr)

PAA238Hu01 酪氨酸酶(TYR)多克隆抗体 Polyclonal Antibody to Tyrosinase (TYR)

PAA238Ra01 酪氨酸酶(TYR)多克隆抗体 Polyclonal Antibody to Tyrosinase (TYR)

PAA238Po01 酪氨酸酶(TYR)多克隆抗体 Polyclonal Antibody to Tyrosinase (TYR)

PAA238Bo01 酪氨酸酶(TYR)多克隆抗体 Polyclonal Antibody to Tyrosinase (TYR)

PAG524Hu01 TYRO蛋白酪氨酸激酶结合蛋白(TYROBP)多克隆抗体 Polyclonal Antibody to TYRO Protein Tyrosine Kinase Binding Protein (TYROBP)

PAG989Mu01 TXK酪氨酸激酶(TXK)多克隆抗体 Polyclonal Antibody to TXK Tyrosine Kinase (TXK)

PAF862Hu01 扭转原肠胚形成同源物1(TWSG1)多克隆抗体 Polyclonal Antibody to Twisted Gastrulation Protein Homolog 1 (TWSG1)

PAC838Hu01 Twist转录因子线粒体转录终止因子1抗体(TWIST)多克隆抗体 Polyclonal Antibody to Twist Transcription Factor (TWIST)

PAM410Hu01 双解丝蛋白1(TWF1)多克隆抗体 Polyclonal Antibody to Twinfilin 1 (TWF1)

PAC814Hu01 肿瘤易感基因101(TSG101)多克隆抗体 Polyclonal Antibody to Tumor Susceptibility Gene 101 (TSG101)

PAG962Mu01 翻译调节肿瘤蛋白1(TPT1)多克隆抗体 Polyclonal Antibody to Tumor Protein, Translationally Controlled 1 (TPT1)