LRRC59蛋白抗体
『别名』Leucine-rich repeat-containing protein 59; LRC59_HUMAN; LRRC59; PRO1855.
『浓度』LRRC59蛋白抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
LRRC59蛋白抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
LRRC59蛋白抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAB702Mu01 WAP四二硫化物核心域蛋白1(WFDC1)多克隆抗体 Polyclonal Antibody to WAP Four Disulfide Core Domain Protein 1 (WFDC1)
PAG523Hu01 V-Yes-1 Yamaguchi肉瘤病毒相关癌基因同源物(LYN)多克隆抗体 Polyclonal Antibody to V-Yes-1 Yamaguchi Sarcoma Viral Related Oncogene Homolog (LYN)
PAK032Hu01 含V-Set域T细胞激活抑制因子1(VTCN1)多克隆抗体 Polyclonal Antibody to V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1)
PAK032Hu02 含V-Set域T细胞激活抑制因子1(VTCN1)多克隆抗体 Polyclonal Antibody to V-Set Domain Containing T-Cell Activation Inhibitor 1 (VTCN1)
PAN894Hu01 含V-Set免疫球蛋白域蛋白2(VSIG2)多克隆抗体 Polyclonal Antibody to V-Set And Immunoglobulin Domain Containing Protein 2 (VSIG2)
PAB826Hu01 V-Rel网状内皮增生病毒癌基因同源物B(RELB)多克隆抗体 Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB)
PAB826Mu01 V-Rel网状内皮增生病毒癌基因同源物B(RELB)多克隆抗体 Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB)
PAA616Hu01 V-Rel网状内皮增生病毒癌基因同源物A(RELA)多克隆抗体 Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)
PAA616Mu01 V-Rel网状内皮增生病毒癌基因同源物A(RELA)多克隆抗体 Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)
PAA616Ra01 V-Rel网状内皮增生病毒癌基因同源物A(RELA)多克隆抗体 Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog A (RELA)
PAP741Mu01 V-Rel网状内皮增生病毒癌基因同源物(REL)多克隆抗体 Polyclonal Antibody to V-Rel Reticuloendotheliosis Viral Oncogene Homolog (REL)
PAM097Hu01 V-Ral猴白血病病毒癌基因同源物A(RALA)多克隆抗体 Polyclonal Antibody to V-Ral Simian Leukemia Viral Oncogene Homolog A (RALA)
PAA950Hu01 血管性血友病因子裂解蛋白酶(vWFCP)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor Cleaving Protease (vWFCP)
PAA694Hu01 含血管性血友病因子A域蛋白3A(vWA3A)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor A Domain Containing Protein 3A (vWA3A)
PAA694Mu01 含血管性血友病因子A域蛋白3A(vWA3A)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor A Domain Containing Protein 3A (vWA3A)
PAB389Hu01 含血管性血友病因子A域蛋白2(vWA2)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor A Domain Containing Protein 2 (vWA2)
PAA841Mu01 含血管性血友病因子A域蛋白1(vWA1)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor A Domain Containing Protein 1 (vWA1)
PAA841Hu01 含血管性血友病因子A域蛋白1(vWA1)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor A Domain Containing Protein 1 (vWA1)
PAA841Ra01 含血管性血友病因子A域蛋白1(vWA1)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor A Domain Containing Protein 1 (vWA1)
PAA841Mu02 含血管性血友病因子A域蛋白1(vWA1)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor A Domain Containing Protein 1 (vWA1)
PAA833Hu01 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Mu01 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Ra01 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Ca01 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Po01 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Rb51 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Ra02 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Mu02 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAA833Hu02 血管性血友病因子(vWF)多克隆抗体 Polyclonal Antibody to Von Willebrand Factor (vWF)
PAJ769Ra01 视网膜血管瘤肿瘤抑制因子(vHL)多克隆抗体 Polyclonal Antibody to Von Hippel Lindau Tumor Suppressor (vHL)
PAG901Hu01 电压依赖性阴离子通道蛋白1(VDAC1)多克隆抗体 Polyclonal Antibody to Voltage Dependent Anion Channel Protein 1 (VDAC1)
PAL768Hu01 肺癌衍生V-Myc骨髓细胞瘤病毒癌基因同源物1(MYCL1)多克隆抗体 Polyclonal Antibody to V-Myc Myelocytomatosis Viral Oncogene Homolog 1, Lung Carcinoma Derived (MYCL1)
PAB290Hu01 V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)多克隆抗体 Polyclonal Antibody to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)
PAB290Ra01 V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)多克隆抗体 Polyclonal Antibody to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)
PAB290Mu01 V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)多克隆抗体 Polyclonal Antibody to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)
PAB290Mu02 V-Myc骨髓细胞瘤病毒癌基因同源物(MYC)多克隆抗体 Polyclonal Antibody to V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC)
PAB055Hu01 V-Myb髓细胞组织增生病毒癌基因同源物(MYB)多克隆抗体 Polyclonal Antibody to V-Myb Myeloblastosis Viral Oncogene Homolog (MYB)
PAB055Mu01 V-Myb髓细胞组织增生病毒癌基因同源物(MYB)多克隆抗体 Polyclonal Antibody to V-Myb Myeloblastosis Viral Oncogene Homolog (MYB)
PAH596Hu01 V-Maf肌肉腱膜纤维肉瘤癌基因同源物(MAF)多克隆抗体 Polyclonal Antibody to V-Maf Musculoaponeurotic Fibrosarcoma Oncogene Homolog (MAF)
PAH751Hu01 V-Ki-Ras2 Kirsten大鼠肉瘤病毒癌基因同源物(KRAS)多克隆抗体 Polyclonal Antibody to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS)
PAB292Ra01 V-Jun肉瘤病毒癌基因同源物(JUN)多克隆抗体 Polyclonal Antibody to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)
PAB292Hu01 V-Jun肉瘤病毒癌基因同源物(JUN)多克隆抗体 Polyclonal Antibody to V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)
PAB041Hu01 玻连蛋白(VTN)多克隆抗体 Polyclonal Antibody to Vitronectin (VTN)
PAB041Hu02 玻连蛋白(VTN)多克隆抗体 Polyclonal Antibody to Vitronectin (VTN)
PAB041Mu01 玻连蛋白(VTN)多克隆抗体 Polyclonal Antibody to Vitronectin (VTN)
PAB041Mu02 玻连蛋白(VTN)多克隆抗体 Polyclonal Antibody to Vitronectin (VTN)
PAB041Ra01 玻连蛋白(VTN)多克隆抗体 Polyclonal Antibody to Vitronectin (VTN)
PAA926Ge01 维生素K1(VK1)多克隆抗体 Polyclonal Antibody to Vitamin K1 (VK1)
PAA920Ge01 维生素D3(VD3)多克隆抗体 Polyclonal Antibody to Vitamin D3 (VD3)
PAA921Ge01 维生素D2(VD2)多克隆抗体 Polyclonal Antibody to Vitamin D2 (VD2)
PAA475Hu01 维生素D受体(VDR)多克隆抗体 Polyclonal Antibody to Vitamin D Receptor (VDR)
PAA475Mu01 维生素D受体(VDR)多克隆抗体 Polyclonal Antibody to Vitamin D Receptor (VDR)
PAA475Mu02 维生素D受体(VDR)多克隆抗体 Polyclonal Antibody to Vitamin D Receptor (VDR)
PAA475Ra01 维生素D受体(VDR)多克隆抗体 Polyclonal Antibody to Vitamin D Receptor (VDR)
PAB810Hu01 维生素D结合蛋白(DBP)多克隆抗体 Polyclonal Antibody to Vitamin D Binding Protein (DBP)
PAB810Mu01 维生素D结合蛋白(DBP)多克隆抗体 Polyclonal Antibody to Vitamin D Binding Protein (DBP)
PAB810Ra01 维生素D结合蛋白LRRC59蛋白抗体(DBP)多克隆抗体 Polyclonal Antibody to Vitamin D Binding Protein (DBP)
PAB810Mu02 维生素D结合蛋白(DBP)多克隆抗体 Polyclonal Antibody to Vitamin D Binding Protein (DBP)
PAB810Ra02 维生素D结合蛋白(DBP)多克隆抗体 Polyclonal Antibody to Vitamin D Binding Protein (DBP)
PAB810Hu02 维生素D结合蛋白(DBP)多克隆抗体 Polyclonal Antibody to Vitamin D Binding Protein (DBP)