?受体蛋白酪氨酸磷酸酶F抗体
『别名』LAR; LAR protein; LARFN5C; LARS; LCA homolog; Leukocyte antigen related (LAR) PTP receptor; Leukocyte antigen related; Leukocyte antigen related PTP receptor; Leukocyte antigen related tyrosine phosphatase; Leukocyte common antigen related; Protein Tyrosine Phosphatase Receptor Type F; Protein tyrosine phosphatase receptor type F polypeptide; Ptprf; PTPRF protein; PTPRF_HUMAN; Receptor linked protein tyrosine phosphatase LAR; Receptor type tyrosine protein phosphatase F; Receptor type tyrosine protein phosphatase F precursor; Receptor-type tyrosine-protein phosphatase F.
『浓度』受体蛋白酪氨酸磷酸酶F抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
受体蛋白酪氨酸磷酸酶F抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
受体蛋白酪氨酸磷酸酶F抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
EL1591c 猪基质金属蛋白酶抑制因子3(TIMP-3)酶联免疫吸附测定试剂盒 Porcine TIMP-3 (Tissue Inhibitors of Metalloproteinase 3) ELISA Kit
EL0608c 猪基质金属蛋白酶抑制因子2(TIMP-2)酶联免疫吸附测定试剂盒 Porcine TIMP-2 (Tissue Inhibitors of Metalloproteinase 2) ELISA Kit
EL0667c 猪基质金属蛋白酶组织抑制因子1(TIMP-1)酶联免疫吸附测定试剂盒 Porcine TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
EL0973c 猪TGF-β诱导早期应答基因1(TIEG1)酶联免疫吸附测定试剂盒 Porcine TIEG1 (TGF Beta Inducible Early Response Gene 1) ELISA Kit
EL0444c 猪胸腺非依赖性抗原(TI-Ag)酶联免疫吸附测定试剂盒 Porcine TI-Ag (Thymus Independent Antigen) ELISA Kit
EL2075c 猪甲状腺原氨酸(Thyronine)酶联免疫吸附测定试剂盒 Porcine Thyronine ELISA Kit
EL0860c 猪甲状腺素受体α(THRα)酶联免疫吸附测定试剂盒 Porcine THRα (Thyroid Hormone Receptor Alpha) ELISA Kit
EL1874c 猪甲状腺激素反应基因(THRSP)酶联免疫吸附测定试剂盒 Porcine THRSP (Thyroid Hormone Responsive) ELISA Kit
EL1895c 猪甲状腺激素自身抗体(THAA)酶联免疫吸附测定试剂盒 Porcine THAA (Thyroid Hormone Autoantibody) ELISA Kit
EL0146c 猪酪氨酸羟化酶(TH)酶联免疫吸附测定试剂盒 Porcine TH (Tyrosine Hydroxylase) ELISA Kit
EL0694c 猪T细胞特异性鸟苷三磷酸酶(Tgtp)酶联免疫吸附测定试剂盒 Porcine Tgtp (T-cell Specific Gtpase) ELISA Kit
EL1310c 猪表皮转谷氨酰胺酶(TGM3)酶联免疫吸附测定试剂盒 Porcine TGM3 (Transglutaminase 3, Epidermal) ELISA Kit
EL0279c 猪组织转谷氨酰胺酶(TGM2)酶联免疫吸附测定试剂盒 Porcine TGM2 (Transglutaminase 2, Tissue) ELISA Kit
EL0037c 猪角质转谷氨酰胺酶(TGM1)酶联免疫吸附测定试剂盒 Porcine TGM1 (Transglutaminase 1, Keratinocyte) ELISA Kit
EL2039c 猪转化生长因子β诱导蛋白(TGFβI)酶联免疫吸附测定试剂盒 Porcine TGFβI (Transforming Growth Factor Beta Induced Protein) ELISA Kit
EL1387c 猪转化生长因子β3(TGF-β3)酶联免疫吸附测定试剂盒 Porcine TGF-β3 (Transforming Growth Factor β3) ELISA Kit
EL0910c 猪转化生长因子β2(TGF-β2)酶联免疫吸附测定试剂盒 Porcine TGF-β2 (Transforming Growth Factor β2) ELISA Kit
EL0009c 猪转化生长因子β1(TGF-β1)酶联免疫吸附测定试剂盒 "Porcine TGF-β1 (Transforming Growth Factor β1) ELISA Kit
"
EL1175c 猪转化生长因子α(TGF-α)酶联免疫吸附测定试剂盒 Porcine TGF-α (Transforming Growth Factor α) ELISA Kit
EL2186c 猪甲状腺球蛋白(TG)酶联免疫吸附测定试剂盒 Porcine TG (Thyroglobulin) ELISA Kit
EL1569c 猪转铁蛋白受体2(TFR2)酶联免疫吸附测定试剂盒 Porcine TFR2 (Transferrin Receptor 2) ELISA Kit
EL1850c 猪转铁蛋白受体(TFR/CD71)酶联免疫吸附测定试剂盒 Porcine TFR/CD71 (Transferrin Receptor) ELISA Kit
EL1029c 猪组织因子途径抑制因子2(TFPI2)酶联免疫吸附测定试剂盒 Porcine TFPI2 (Tissue Factor Pathway Inhibitor 2) ELISA Kit
EL0846c 猪组织因子途径抑制因子(TFPI)酶联免疫吸附测定试剂盒 Porcine TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit
EL1916c 猪肠三叶肽因子3(TFF3)酶联免疫吸附测定试剂盒 Porcine TFF3 (Trefoil Factor 3, Intestinal ) ELISA Kit
EL0957c 猪三叶因子2(TFF2)酶联免疫吸附测定试剂盒 Porcine TFF2 (Trefoil Factor 2) ELISA Kit
EL0591c 猪三叶因子1(TFF1)酶联免疫吸附测定试剂盒 Porcine TFF1 (Trefoil Factor 1) ELISA Kit
EL0873c 猪转移因子(TF)酶联免疫吸附测定试剂盒 Porcine TF (Transfer Factor) ELISA Kit
EL0100c 猪组织因子(TF)酶联免疫吸附测定试剂盒 Porcine TF (Tissue Factor) ELISA Kit
EL0754c 猪端粒酶逆转录酶(TERT)酶联免疫吸附测定试剂盒 Porcine TERT (Telomerase Reverse Transcriptase) ELISA Kit
EL1891c 猪端粒酶相关蛋白1(TEP1)酶联免疫吸附测定试剂盒 Porcine TEP1 (Telomerase Associated Protein 1) ELISA Kit
EL1018c 猪促甲状腺激素胚胎因子(TEF)酶联免疫吸附测定试剂盒 Porcine TEF (Thyrotroph Embryonic Factor) ELISA Kit
EL0462c 猪胸腺表达趋化因子(TECK/CCL25)酶联免疫吸附测定试剂盒 Porcine TECK/CCL25 (Thymus Expressed Chemokine) ELISA Kit
EL0327c 猪端粒酶(TE)酶联免疫吸附测定试剂盒 Porcine TE (Telomerase) ELISA Kit
EL0172c 猪末端脱氧核糖核酸转移酶(TDT)酶联免疫吸附测定试剂盒 Porcine TDT (Terminal Deoxynucleotidyl Transferase) ELISA Kit
EL2027c 猪43KDa Tar DNA结合蛋白(TDP43)酶联免疫吸附测定试剂盒 Porcine TDP43 (Tar DNA Binding Protein 43KDa ) ELISA Kit
EL0030c 猪畸胎瘤衍生生长因子1(TDGF1)酶联免疫吸附测定试剂盒 Porcine TDGF1 (Teratocarcinoma Derived Growth Factor 1) ELISA Kit
EL0586c 猪胸腺依赖性抗原(TD-Ag)酶联免疫吸附测定试剂盒 Porcine TD-Ag (Thymus Dependent Antigen) ELISA Kit
EL1272c 猪T细胞受体(TCR)酶联免疫吸附测定试剂盒 Porcine TCR (Tcell Receptor) ELISA Kit
EL1713c 猪T细胞生长因子-Ⅲ(TCGF-Ⅲ)酶联免疫吸附测定试剂盒 Porcine TCGF-Ⅲ (Tcell Growth Factor Ⅲ) ELISA Kit
EL2021c 猪转录因子20(TCF20)酶联免疫吸附测定试剂盒 Porcine TCF20 (Transcription Factor 20) ELISA Kit
EL0485c 猪末端补体复合物C5b-9(TCC C5b-9)酶联免疫吸附测定试剂盒 Porcine TCC C5b-9 (Terminal Complement Complex C5b-9) ELISA Kit
EL0682c 猪硫氧还蛋白结合蛋白2(TBP2)酶联免疫吸附测定试剂盒 Porcine TBP2 (Thioredoxin Binding Protein 2) ELISA Kit
EL1934c 猪TATA结合蛋白(TBP)酶联免疫吸附测定试剂盒 Porcine TBP (TATA Binding Protein) ELISA Kit
EL1123c 猪甲状腺结合球蛋白(TBG)酶联免疫吸附测定试剂盒 Porcine TBG (Thyroxine Binding Globulin) ELISA Kit
EL2168c 猪凝血酶/抗凝血酶复合物(TAT)酶联免疫吸附测定试剂盒 Porcine TAT (Thrombin/Antithrombin Complex) ELISA Kit
EL1527c 猪苏氨酰tRNA合成酶(TARS)酶联免疫吸附测定试剂盒 Porcine TARS (Threonyl tRNA Synthetase, cytoplasmic) ELISA Kit
EL0691c 猪胸腺激活调节趋化因子(TARC)酶联免疫吸附测定试剂盒 Porcine TARC (Thymus Activation Regulated Chemokine) ELISA Kit
EL0413c 猪胰蛋白酶原激活肽(TAP)酶联免疫吸附测定试剂盒 Porcine TAP (Trypsinogen Activation Peptide) ELISA Kit
EL1459c 猪抗原提呈相关转运蛋白(TAP)酶联免疫吸附测定试剂盒 Porcine TAP (Transporter Associated with Antigen Processing) ELISA Kit
EL1640c 猪T细胞急性淋巴母细胞白血病相关抗原(TALLA-1/CD231)酶联免疫吸附测定试剂盒 Porcine TALLA-1 (T-cell Acute Lymphoblastic Leukemia Antigen) ELISA Kit
EL0418c 猪转胶蛋白(TAGLN)酶联免疫吸附测定试剂盒 Porcine TAGLN (Transgelin) ELISA Kit
EL0204c 猪纤溶抑制因子(TAFI)酶联免疫吸附测定试剂盒 Porcine TAFI (Thrombin Activatable Fibrinolysis Inhibitor) ELISA Kit
EL0977c 猪TATA框结合蛋白相关因子2(TAF2)酶联免疫吸附测定试剂盒 Porcine TAF2 (TATA Box Binding Protein Associated Factor 2) ELISA Kit
EL0317c 猪TATA框结合蛋白相关因子13(TAF13)酶联免疫吸附测定试剂盒 Porcine TAF13 (TATA Box Binding Protein Associated Factor 13) ELISA Kit
EL0475c 猪TATA框结合蛋白相关因子1(TAF1)酶联免疫吸附测定试剂盒 Porcine TAF1 (TATA Box Binding Protein Associated Factor 1) ELISA Kit
EL2218c 猪TATA框结合蛋白/TBP相关因子(TAF)酶联免疫吸附测定试剂盒 Porcine TAF (TATA Box Binding Protein/TBP-Associated Factors) ELISA Kit
EL0452c 猪速激肽受体3受体蛋白酪氨酸磷酸酶F抗体(TACR3)酶联免疫吸附测定试剂盒 Porcine TACR3 (Tachykinin Receptor 3) ELISA Kit
EL0384c 猪速激肽受体2(TACR2)酶联免疫吸附测定试剂盒 Porcine TACR2 (Tachykinin Receptor 2) ELISA Kit
EL1096c 猪速激肽受体1(TACR1)酶联免疫吸附测定试剂盒 Porcine TACR1 (Tachykinin Receptor 1) ELISA Kit