?鸡传染性法氏囊病病毒抗体
『别名』Infectious Bursal Disease Vaccine; infectious bursal disease; IBD.
『浓度』鸡传染性法氏囊病病毒抗体1mg/1ml
『规格』1ml/1mg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
鸡传染性法氏囊病病毒抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
鸡传染性法氏囊病病毒抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA386Ra01 脂蛋白脂酶(LIPD)多克隆抗体 Polyclonal Antibody to Lipase, Lipoprotein (LIPD)
PAA386Mu02 脂蛋白脂酶(LIPD)多克隆抗体 Polyclonal Antibody to Lipase, Lipoprotein (LIPD)
PAB276Hu01 激素敏感性脂肪酶(LIPE)多克隆抗体 Polyclonal Antibody to Lipase, Hormone Sensitive (LIPE)
PAB276Ra01 激素敏感性脂肪酶(LIPE)多克隆抗体 Polyclonal Antibody to Lipase, Hormone Sensitive (LIPE)
PAA769Hu01 肝脂酶(LIPC)多克隆抗体 Polyclonal Antibody to Lipase, Hepatic (LIPC)
PAA769Mu01 肝脂酶(LIPC)多克隆抗体 Polyclonal Antibody to Lipase, Hepatic (LIPC)
PAA769Ra01 肝脂酶(LIPC)多克隆抗体 Polyclonal Antibody to Lipase, Hepatic (LIPC)
PAA769Po01 肝脂酶(LIPC)多克隆抗体 Polyclonal Antibody to Lipase, Hepatic (LIPC)
PAA769Hu02 肝脂酶(LIPC)多克隆抗体 Polyclonal Antibody to Lipase, Hepatic (LIPC)
PAA769Mu02 肝脂酶(LIPC)多克隆抗体 Polyclonal Antibody to Lipase, Hepatic (LIPC)
PAD226Hu01 胃脂酶(LIPF)多克隆抗体 Polyclonal Antibody to Lipase, Gastric (LIPF)
PAD226Mu01 胃脂酶(LIPF)多克隆抗体 Polyclonal Antibody to Lipase, Gastric (LIPF)
PAD226Ra01 胃脂酶(LIPF)多克隆抗体 Polyclonal Antibody to Lipase, Gastric (LIPF)
PAD226Po01 胃脂酶(LIPF)多克隆抗体 Polyclonal Antibody to Lipase, Gastric (LIPF)
PAA469Hu01 内皮脂肪酶(LIPG)多克隆抗体 Polyclonal Antibody to Lipase, Endothelial (LIPG)
PAA469Mu01 内皮脂肪酶(LIPG)多克隆抗体 Polyclonal Antibody to Lipase, Endothelial (LIPG)
PAA469Ra01 内皮脂肪酶(LIPG)多克隆抗体 Polyclonal Antibody to Lipase, Endothelial (LIPG)
PAA469Mu02 内皮脂肪酶(LIPG)多克隆抗体 Polyclonal Antibody to Lipase, Endothelial (LIPG)
PAC342Hu01 胆盐依赖性脂肪酶(BSDL)多克隆抗体 Polyclonal Antibody to Lipase, Bile Salt Dependent (BSDL)
PAE179Hu01 脂肪酶成熟因子2(LMF2)多克隆抗体 Polyclonal Antibody to Lipase Maturation Factor 2 (LMF2)
PAE177Hu01 脂肪酶成熟因子1(LMF1)多克隆抗体 Polyclonal Antibody to Lipase Maturation Factor 1 (LMF1)
PAE176Hu01 脂肪酶I(LIPI)多克隆抗体 Polyclonal Antibody to Lipase I (LIPI)
PAE176Mu01 脂肪酶I(LIPI)多克隆抗体 Polyclonal Antibody to Lipase I (LIPI)
PAE176Ra01 脂肪酶I(LIPI)多克隆抗体 Polyclonal Antibody to Lipase I (LIPI)
PAE175Hu01 脂肪酶H(LIPH)多克隆抗体 Polyclonal Antibody to Lipase H (LIPH)
PAE175Mu01 脂肪酶H(LIPH)多克隆抗体 Polyclonal Antibody to Lipase H (LIPH)
PAD225Hu01 溶酶体酸脂肪酶A(LIPA)多克隆抗体 Polyclonal Antibody to Lipase A, Lysosomal Acid (LIPA)
PAD225Mu01 溶酶体酸脂肪酶A(LIPA)多克隆抗体 Polyclonal Antibody to Lipase A, Lysosomal Acid (LIPA)
PAD225Ra01 溶酶体酸脂肪酶A(LIPA)多克隆抗体 Polyclonal Antibody to Lipase A, Lysosomal Acid (LIPA)
PAD225Mu02 溶酶体酸脂肪酶A(LIPA)多克隆抗体 Polyclonal Antibody to Lipase A, Lysosomal Acid (LIPA)
PAP555Hu01 T-细胞激活连接蛋白家族成员2(LAT2)多克隆抗体 Polyclonal Antibody to Linker For Activation Of T-Cells Family, Member 2 (LAT2)
PAP555Ra01 T-细胞激活连接蛋白家族成员2(LAT2)多克隆抗体 Polyclonal Antibody to Linker For Activation Of T-Cells Family, Member 2 (LAT2)
PAP555Mu01 T-细胞激活连接蛋白家族成员2(LAT2)多克隆抗体 Polyclonal Antibody to Linker For Activation Of T-Cells Family, Member 2 (LAT2)
PAA270Hu01 T-细胞激活连接蛋白(LAT)多克隆抗体 Polyclonal Antibody to Linker For Activation Of T-Cell (LAT)
PAA270Mu01 T-细胞激活连接蛋白(LAT)多克隆抗体 Polyclonal Antibody to Linker For Activation Of T-Cell (LAT)
PAA270Ra01 T-细胞激活连接蛋白(LAT)多克隆抗体 Polyclonal Antibody to Linker For Activation Of T-Cell (LAT)
PAM546Mu01 Lin-28同源物A(LIN28A)多克隆抗体 Polyclonal Antibody to Lin-28 Homolog A (LIN28A)
PAM546Mu02 Lin-28同源物A(LIN28A)多克隆抗体 Polyclonal Antibody to Lin-28 Homolog A (LIN28A)
PAJ524Hu01 LIM域结合蛋白1(LDB1)多克隆抗体 Polyclonal Antibody to LIM Domain Binding Protein 1 (LDB1)
PAJ738Hu01 LIM和SH3蛋白1(LASP1)多克隆抗体 Polyclonal Antibody to LIM And SH3 Protein 1 (LASP1)
PAP882Hu01 LIM衰老细胞抗原样域蛋白1(LIMS1)多克隆抗体 Polyclonal Antibody to LIM And Senescent Cell Antigen Like Domains Protein 1 (LIMS1)
PAH936Hu01 样糖基转移酶(LARGE)多克隆抗体 Polyclonal Antibody to Like Glycosyltransferase (LARGE)
PAD236Hu01 白三烯A4水解酶(LTA4H)多克隆抗体 Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H)
PAD236Mu01 白三烯A4水解酶(LTA4H)多克隆抗体 Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H)
PAD236Mu02 白三烯A4水解酶(LTA4H)多克隆抗体 Polyclonal Antibody to Leukotriene A4 Hydrolase (LTA4H)
PAB399Hu01 白细胞免疫球蛋白样受体亚家族B成员4(LILRB4)多克隆抗体 Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4)
PAB399Mu01 白细胞免疫球蛋白样受体亚家族B成员4(LILRB4)多克隆抗体 Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4 (LILRB4)
PAB507Hu01 白细胞免疫球蛋白样受体亚家族B成员3(LILRB3)多克隆抗体 Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 3 (LILRB3)
PAB507Mu01 白细胞免疫球蛋白样受体亚家族B成员3(LILRB3)多克隆抗体 Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 3 (LILRB3)
PAB395Hu01 白细胞免疫球蛋白样受体亚家族B成员1(LILRB1)多克隆抗体 Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 1 (LILRB1)
PAB387Hu01 白细胞免疫球蛋白样受体亚家族A成员3(LILRA3)多克隆抗体 Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 3 (LILRA3)
PAB394Hu01 白细胞免疫球蛋白样受体亚家族A成员2(LILRA2)多克隆抗体 Polyclonal Antibody to Leukocyte Immunoglobulin Like Receptor Subfamily A, Member 2 (LILRA2)
PAD387Mu01 白细胞弹性蛋白酶抑制因子(LEI)多克隆抗体 Polyclonal Antibody to Leukocyte Elastase Inhibitor (LEI)
PAD387Hu01 白细胞弹性蛋白酶抑制因子(LEI)多克隆抗体 Polyclonal Antibody to Leukocyte Elastase Inhibitor (LEI)
PAD387Mu02 鸡传染性法氏囊病病毒抗体白细胞弹性蛋白酶抑制因子(LEI)多克隆抗体 Polyclonal Antibody to Leukocyte Elastase Inhibitor (LEI)
PAD387Ra01 白细胞弹性蛋白酶抑制因子(LEI)多克隆抗体 Polyclonal Antibody to Leukocyte Elastase Inhibitor (LEI)
PAF541Hu01 白细胞衍生趋化因子2(LECT2)多克隆抗体 Polyclonal Antibody to Leukocyte Cell Derived Chemotaxin 2 (LECT2)
PAF541Ra01 白细胞衍生趋化因子2(LECT2)多克隆抗体 Polyclonal Antibody to Leukocyte Cell Derived Chemotaxin 2 (LECT2)