?胰岛素样生长因子结合蛋白9抗体
『别名』CCN family member 3; IBP-9; IGF-binding protein 9; IGFBP-9; IGFBP9; Insulin-like growth factor-binding protein 9; Nephroblastoma overexpressed gene protein homolog; Nephroblastoma-overexpressed gene protein homolog; NOV; NOV_HUMAN; NovH; Protein NOV homolog.
『浓度』胰岛素样生长因子结合蛋白9抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
胰岛素样生长因子结合蛋白9抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
胰岛素样生长因子结合蛋白9抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAB010Mu02 低密度脂蛋白受体相关蛋白1(LRP1)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein Receptor Related Protein 1 (LRP1)
PAD104Mu01 低密度脂蛋白受体衔接蛋白1(LDLRAP1)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1)
PAD104Mu02 低密度脂蛋白受体衔接蛋白1(LDLRAP1)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein Receptor Adaptor Protein 1 (LDLRAP1)
PAB008Hu01 低密度脂蛋白受体(LDLR)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein Receptor (LDLR)
PAB008Mu01 低密度脂蛋白受体(LDLR)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein Receptor (LDLR)
PAB008Mu02 低密度脂蛋白受体(LDLR)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein Receptor (LDLR)
PAB008Ra01 低密度脂蛋白受体(LDLR)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein Receptor (LDLR)
PAB107Hu01 低密度脂蛋白(LDL)多克隆抗体 Polyclonal Antibody to Low Density Lipoprotein (LDL)
PAC568Hu01 兜甲蛋白(LOR)多克隆抗体 Polyclonal Antibody to Loricrin (LOR)
PAP312Hu01 长寿保证同源物2(LASS2)多克隆抗体 Polyclonal Antibody to Longevity Assurance Homolog 2 (LASS2)
PAC044Hu01 肝X受体α(LXRα)多克隆抗体 Polyclonal Antibody to Liver X Receptor Alpha (LXRa)
PAC044Mu01 肝X受体α(LXRα)多克隆抗体 Polyclonal Antibody to Liver X Receptor Alpha (LXRa)
PAC044Mu02 肝X受体α(LXRα)多克隆抗体 Polyclonal Antibody to Liver X Receptor Alpha (LXRa)
PAC044Bo01 肝X受体α(LXRα)多克隆抗体 Polyclonal Antibody to Liver X Receptor Alpha (LXRa)
PAD965Hu01 肝受体同源物1(LRH1)多克隆抗体 Polyclonal Antibody to Liver Receptor Homolog 1 (LRH1)
PAD965Ra01 肝受体同源物1(LRH1)多克隆抗体 Polyclonal Antibody to Liver Receptor Homolog 1 (LRH1)
PAH750Hu01 肝表达抗菌肽2(LEAP2)多克隆抗体 Polyclonal Antibody to Liver Expressed Antimicrobial Peptide 2 (LEAP2)
PAA842Hu01 脂蛋白a(Lpa)多克隆抗体 Polyclonal Antibody to Lipoprotein, a (Lpa)
PAB406Bo01 脂多糖结合蛋白(LBP)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP)
PAB406Hu01 脂多糖结合蛋白(LBP)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP)
PAB406Mu01 脂多糖结合蛋白(LBP)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP)
PAB406Ra01 脂多糖结合蛋白(LBP)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP)
PAB406Mu02 脂多糖结合蛋白(LBP)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP)
PAB406Po01 脂多糖结合蛋白(LBP)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP)
PAB406Rb51 脂多糖结合蛋白(LBP)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide Binding Protein (LBP)
PAB526Ge01 脂多糖(LPS)多克隆抗体 Polyclonal Antibody to Lipopolysaccharide (LPS)
PAE174Hu01 前列腺蛋白类脂肪酶B(LIPB)多克隆抗体 Polyclonal Antibody to Lipophilin B, Prostatein Like (LIPB)
PAD744Hu01 脂解激活脂蛋白受体(LSR)多克隆抗体 Polyclonal Antibody to Lipolysis Stimulated Lipoprotein Receptor (LSR)
PAD744Mu01 脂解激活脂蛋白受体(LSR)多克隆抗体 Polyclonal Antibody to Lipolysis Stimulated Lipoprotein Receptor (LSR)
PAD744Mu02 脂解激活脂蛋白受体(LSR)多克隆抗体 Polyclonal Antibody to Lipolysis Stimulated Lipoprotein Receptor (LSR)
PAS409Ra01 质载脂蛋白样蛋白1(LCNL1)多克隆抗体 Polyclonal Antibody to Lipocalin Like Protein 1 (LCNL1)
PAE144Hu01 脂质运载蛋白9(LCN9)多克隆抗体 Polyclonal Antibody to Lipocalin 9 (LCN9)
PAE144Ra01 脂质运载蛋白9(LCN9)多克隆抗体 Polyclonal Antibody to Lipocalin 9 (LCN9)
PAE145Ra01 脂质运载蛋白8(LCN8)多克隆抗体 Polyclonal Antibody to Lipocalin 8 (LCN8)
PAE145Mu01 脂质运载蛋白8(LCN8)多克隆抗体 Polyclonal Antibody to Lipocalin 8 (LCN8)
PAE146Hu01 脂质运载蛋白6(LCN6)多克隆抗体 Polyclonal Antibody to Lipocalin 6 (LCN6)
PAE146Ra01 脂质运载蛋白6(LCN6)多克隆抗体 Polyclonal Antibody to Lipocalin 6 (LCN6)
PAE146Hu02 脂质运载蛋白6(LCN6)多克隆抗体 Polyclonal Antibody to Lipocalin 6 (LCN6)
PAS457Ra01 脂质运载蛋白5(LCN5)多克隆抗体 Polyclonal Antibody to Lipocalin 5 (LCN5)
PAS457Mu01 脂质运载蛋白5(LCN5)多克隆抗体 Polyclonal Antibody to Lipocalin 5 (LCN5)
PAS456Mu01 脂质运载蛋白4(LCN4)多克隆抗体 Polyclonal Antibody to Lipocalin 4 (LCN4)
PAS388Hu01 脂质运载蛋白15(LCN15)多克隆抗体 Polyclonal Antibody to Lipocalin 15 (LCN15)
PAG484Hu01 脂质运载蛋白12(LCN12)多克隆抗体 Polyclonal Antibody to Lipocalin 12 (LCN12)
PAG484Mu01 脂质运载蛋白12(LCN12)多克隆抗体 Polyclonal Antibody to Lipocalin 12 (LCN12)
PAG484Ra01 脂质运载蛋白12(LCN12)多克隆抗体 Polyclonal Antibody to Lipocalin 12 (LCN12)
PAB706Hu01 脂质运载蛋白1(LCN1)多克隆抗体 Polyclonal Antibody to Lipocalin 1 (LCN1)
PAB706Mu01 脂质运载蛋白1(LCN1)多克隆抗体 Polyclonal Antibody to Lipocalin 1 (LCN1)
PAB706Ra01 脂质运载蛋白1(LCN1)多克隆抗体 Polyclonal Antibody to Lipocalin 1 (LCN1)
PAG501Mu01 脂素1(LPIN1)多克隆抗体 Polyclonal Antibody to Lipin 1 (LPIN1)
PAG501Ra01 脂素1(LPIN1)多克隆抗体 Polyclonal Antibody to Lipin 1 (LPIN1)
PAG501Mu02 脂素1(LPIN1)多克隆抗体 Polyclonal Antibody to Lipin 1 (LPIN1)
PAB453Ra01 胰脂肪酶(PL)多克隆抗体 Polyclonal Antibody to Lipase, Pancreatic (PL)
PAB453Mu01 胰脂肪酶胰岛素样生长因子结合蛋白9抗体(PL)多克隆抗体 Polyclonal Antibody to Lipase, Pancreatic (PL)
PAB453Mu02 胰脂肪酶(PL)多克隆抗体 Polyclonal Antibody to Lipase, Pancreatic (PL)
PAB453Hu01 胰脂肪酶(PL)多克隆抗体 Polyclonal Antibody to Lipase, Pancreatic (PL)
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