?白介素4抗体
『别名』B cell growth factor 1; B cell IgG differentiation factor; B Cell Stimulatory Factor 1; BCGF 1; BCGF1; Binetrakin; BSF 1; BSF1; HCGF; Hodgkin’s Cell Growth Factor; IA Inducing Factor; IGG1 induction factor; IL 4; Il4e12; Interleukin 4; Interleukin 4, isoform 1; Interleukin4; Lymphocyte stimulatory factor 1; Macrophage fusion factor; Mast cell growth factor 2; MCGF2; MFF; MGC79402; Pitrakinra; T cell growth factor 2; TCGF2; IL4_HUMAN; Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1.
『浓度』白介素4抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素4抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素4抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA093Po01 巨噬细胞炎性蛋白1β(MIP1β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Beta (MIP1b)
PAA093Ra01 巨噬细胞炎性蛋白1β(MIP1β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Beta (MIP1b)
PAA093Rb51 巨噬细胞炎性蛋白1β(MIP1β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Beta (MIP1b)
PAA093Si01 巨噬细胞炎性蛋白1β(MIP1β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Beta (MIP1b)
PAA093Mu02 巨噬细胞炎性蛋白1β(MIP1β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Beta (MIP1b)
PAA092Bo01 巨噬细胞炎性蛋白1α(MIP1α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
PAA092Ca01 巨噬细胞炎性蛋白1α(MIP1α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
PAA092Hu01 巨噬细胞炎性蛋白1α(MIP1α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
PAA092Mu01 巨噬细胞炎性蛋白1α(MIP1α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
PAA092Ra01 巨噬细胞炎性蛋白1α(MIP1α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
PAA092Mu02 巨噬细胞炎性蛋白1α(MIP1α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
PAA092Hu02 巨噬细胞炎性蛋白1α(MIP1α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Alpha (MIP1a)
PAB352Ra01 巨噬细胞表达基因1蛋白(MPG1)多克隆抗体 Polyclonal Antibody to Macrophage Expressed Protein 1 (MPG1)
PAB352Hu01 巨噬细胞表达基因1蛋白(MPG1)多克隆抗体 Polyclonal Antibody to Macrophage Expressed Protein 1 (MPG1)
PAB352Mu01 巨噬细胞表达基因1蛋白(MPG1)多克隆抗体 Polyclonal Antibody to Macrophage Expressed Protein 1 (MPG1)
PAB436Hu01 巨噬细胞幼红细胞黏附蛋白(MAEA)多克隆抗体 Polyclonal Antibody to Macrophage Erythroblast Attacher (MAEA)
PAA091Bo01 巨噬细胞来源趋化因子(MDC)多克隆抗体 Polyclonal Antibody to Macrophage Derived Chemokine (MDC)
PAA091Hu01 巨噬细胞来源趋化因子(MDC)多克隆抗体 Polyclonal Antibody to Macrophage Derived Chemokine (MDC)
PAA091Mu01 巨噬细胞来源趋化因子(MDC)多克隆抗体 Polyclonal Antibody to Macrophage Derived Chemokine (MDC)
PAA091Ra01 巨噬细胞来源趋化因子(MDC)多克隆抗体 Polyclonal Antibody to Macrophage Derived Chemokine (MDC)
PAA091Mu02 巨噬细胞来源趋化因子(MDC)多克隆抗体 Polyclonal Antibody to Macrophage Derived Chemokine (MDC)
PAA091Ca01 巨噬细胞来源趋化因子(MDC)多克隆抗体 Polyclonal Antibody to Macrophage Derived Chemokine (MDC)
PAU056Mu01 含Mab21域蛋白1(MB21D1)多克隆抗体 Polyclonal Antibody to Mab21 Domain Containing Protein 1 (MB21D1)
PAU056Hu01 含Mab21域蛋白1(MB21D1)多克隆抗体 Polyclonal Antibody to Mab21 Domain Containing Protein 1 (MB21D1)
PAD002Hu01 赖氨酸tRNA合成酶(KARS)多克隆抗体 Polyclonal Antibody to Lysyl tRNA Synthetase (KARS)
PAD002Mu01 赖氨酸tRNA合成酶(KARS)多克隆抗体 Polyclonal Antibody to Lysyl tRNA Synthetase (KARS)
PAD002Mu02 赖氨酸tRNA合成酶(KARS)多克隆抗体 Polyclonal Antibody to Lysyl tRNA Synthetase (KARS)
PAF554Hu01 赖氨酰氧化酶样蛋白4(LOXL4)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 4 (LOXL4)
PAF554Hu02 赖氨酰氧化酶样蛋白4(LOXL4)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 4 (LOXL4)
PAF553Hu01 赖氨酰氧化酶样蛋白3(LOXL3)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 3 (LOXL3)
PAF553Mu01 赖氨酰氧化酶样蛋白3(LOXL3)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 3 (LOXL3)
PAF552Hu01 赖氨酰氧化酶样蛋白2(LOXL2)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 2 (LOXL2)
PAF552Mu01 赖氨酰氧化酶样蛋白2(LOXL2)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 2 (LOXL2)
PAF552Ra01 赖氨酰氧化酶样蛋白2(LOXL2)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 2 (LOXL2)
PAF552Ra02 赖氨酰氧化酶样蛋白2(LOXL2)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 2 (LOXL2)
PAF552Hu02 赖氨酰氧化酶样蛋白2(LOXL2)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 2 (LOXL2)
PAF551Hu01 赖氨酰氧化酶样蛋白1(LOXL1)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 1 (LOXL1)
PAF551Mu01 赖氨酰氧化酶样蛋白1(LOXL1)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 1 (LOXL1)
PAF551Hu02 赖氨酰氧化酶样蛋白1(LOXL1)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 1 (LOXL1)
PAF551Ra01 赖氨酰氧化酶样蛋白1(LOXL1)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 1 (LOXL1)
PAF551Mu02 赖氨酰氧化酶样蛋白1(LOXL1)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase Like Protein 1 (LOXL1)
PAC580Hu01 赖氨酰氧化酶(LOX)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase (LOX)
PAC580Mu01 赖氨酰氧化酶(LOX)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase (LOX)
PAC580Mu02 赖氨酰氧化酶(LOX)多克隆抗体 Polyclonal Antibody to Lysyl Oxidase (LOX)
PAB193Hu01 溶菌酶(LZM)多克隆抗体 Polyclonal Antibody to Lysozyme (LZM)
PAB193Mu01 溶菌酶(LZM)多克隆抗体 Polyclonal Antibody to Lysozyme (LZM)
PAB193Ra01 溶菌酶(LZM)多克隆抗体 Polyclonal Antibody to Lysozyme (LZM)
PAB193Rb51 溶菌酶(LZM)多克隆抗体 Polyclonal Antibody to Lysozyme (LZM)
PAB193Bo01 溶菌酶(LZM)多克隆抗体 Polyclonal Antibody to Lysozyme (LZM)
PAB193Ga01 溶菌酶(LZM)多克隆抗体 Polyclonal Antibody to Lysozyme (LZM)
PAB193Mu02 溶菌酶(LZM)多克隆抗体 Polyclonal Antibody to Lysozyme (LZM)
PAB464Hu01 溶酶体关联膜蛋白2(LAMP2)多克隆抗体 Polyclonal Antibody to Lysosomal Associated Membrane Protein 2 (LAMP2)
PAB464Ra01 溶酶体关联膜蛋白2(LAMP2)多克隆抗体 Polyclonal Antibody to Lysosomal Associated Membrane Protein 2 (LAMP2)
PAB464Hu02 溶酶体关联膜蛋白2(LAMP2)多克隆抗体 Polyclonal Antibody to Lysosomal Associated Membrane Protein 2 (LAMP2)
PAB464Mu01 溶酶体关联膜蛋白2(LAMP2)多克隆抗体 Polyclonal Antibody to Lysosomal Associated Membrane Protein 2 (LAMP2)
PAB441Hu01 溶酶体关联膜蛋白1(LAMP1)多克隆抗体 Polyclonal Antibody to Lysosomal Associated Membrane Protein 1 (LAMP1)
PAG532Mu01 溶血卵磷脂酰基转移酶4(LPCAT4)多克隆抗体 Polyclonal Antibody to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4)
PAG532Mu02 溶血卵磷脂酰基转移酶4(LPCAT4)多克隆抗体 Polyclonal Antibody to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4)
PAG532Hu01 溶血卵磷脂酰基转移酶4(LPCAT4)多克隆抗体 Polyclonal Antibody to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4)
PAG531Hu01 白介素4抗体溶血卵磷脂酰基转移酶3(LPCAT3)多克隆抗体 Polyclonal Antibody to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3)
PAG531Mu01 溶血卵磷脂酰基转移酶3(LPCAT3)多克隆抗体 Polyclonal Antibody to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3)
PAG531Mu02 溶血卵磷脂酰基转移酶3(LPCAT3)多克隆抗体 Polyclonal Antibody to Lysophosphatidylcholine Acyltransferase 3 (LPCAT3)