白介素12抗体
『别名』IL12; CLMF p35; CLMF1; CTL maturation factor (TcMF); Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL 12 subunit p35; IL12A; Interleukin 12 alpha chain; Interleukin 12 p35; Interleukin 12A; Natural killer cell stimulatory factor 1; NK cell stimulatory factor chain 1; NKSF1; p35; IL12A_HUMAN.
『浓度』白介素12抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素12抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素12抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAB764Hu01 主要穹窿蛋白(MVP)多克隆抗体 Polyclonal Antibody to Major Vault Protein (MVP)
PAB369Hu01 Ⅱ类主要组织相容性复合体不变链(MHCDG)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II Invariant Chain (MHCDG)
PAB369Mu01 Ⅱ类主要组织相容性复合体不变链(MHCDG)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II Invariant Chain (MHCDG)
PAC167Hu01 Ⅱ类主要组织相容性复合体DRβ1(MHCDRβ1)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1)
PAC167Mu01 Ⅱ类主要组织相容性复合体DRβ1(MHCDRβ1)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1)
PAC167Mu02 Ⅱ类主要组织相容性复合体DRβ1(MHCDRβ1)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Beta 1 (MHCDRb1)
PAC166Hu01 Ⅱ类主要组织相容性复合体DRα(MHCDRα)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)
PAC166Si01 Ⅱ类主要组织相容性复合体DRα(MHCDRα)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)
PAC166Bo01 Ⅱ类主要组织相容性复合体DRα(MHCDRα)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)
PAC166Ca01 Ⅱ类主要组织相容性复合体DRα(MHCDRα)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)
PAC166Po01 Ⅱ类主要组织相容性复合体DRα(MHCDRα)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)
PAC166Rb51 Ⅱ类主要组织相容性复合体DRα(MHCDRα)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DR Alpha (MHCDRa)
PAC165Hu01 Ⅱ类主要组织相容性复合体DQβ1(MHCDQβ1)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1)
PAC165Mu01 Ⅱ类主要组织相容性复合体DQβ1(MHCDQβ1)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1)
PAC165Mu02 Ⅱ类主要组织相容性复合体DQβ1(MHCDQβ1)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1)
PAB856Hu01 Ⅰ类主要组织相容性复合体G(MHCG)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class I G (MHCG)
PAB752Hu01 Ⅰ类主要组织相容性复合体E(MHCE)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class I E (MHCE)
PAC086Hu01 Ⅰ类主要组织相容性复合体C(MHCC)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class I C (MHCC)
PAC092Hu01 Ⅰ类主要组织相容性复合体B(MHCB)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class I B (MHCB)
PAC085Rb51 Ⅰ类主要组织相容性复合体A(MHCA)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class I A (MHCA)
PAC085Hu01 Ⅰ类主要组织相容性复合体A(MHCA)多克隆抗体 Polyclonal Antibody to Major Histocompatibility Complex Class I A (MHCA)
PAB650Hu01 主要碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Major Basic Protein (MBP)
PAB650Mu01 主要碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Major Basic Protein (MBP)
PAB650Ra01 主要碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Major Basic Protein (MBP)
PAB650Po01 主要碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Major Basic Protein (MBP)
PAB650Bo01 主要碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Major Basic Protein (MBP)
PAB650Mu02 主要碱性蛋白(MBP)多克隆抗体 Polyclonal Antibody to Major Basic Protein (MBP)
PAB121Mu01 巨噬细胞刺激蛋白(MSP)多克隆抗体 Polyclonal Antibody to Macrophage Stimulating Protein (MSP)
PAB121Hu01 巨噬细胞刺激蛋白(MSP)多克隆抗体 Polyclonal Antibody to Macrophage Stimulating Protein (MSP)
PAB121Mu02 巨噬细胞刺激蛋白(MSP)多克隆抗体 Polyclonal Antibody to Macrophage Stimulating Protein (MSP)
PAB121Ra01 巨噬细胞刺激蛋白(MSP)多克隆抗体 Polyclonal Antibody to Macrophage Stimulating Protein (MSP)
PAB525Hu01 巨噬细胞刺激1受体(MST1R)多克隆抗体 Polyclonal Antibody to Macrophage Stimulating 1 Receptor (MST1R)
PAB525Mu01 巨噬细胞刺激1受体(MST1R)多克隆抗体 Polyclonal Antibody to Macrophage Stimulating 1 Receptor (MST1R)
PAB591Hu01 巨噬细胞清道夫受体1(MSR1)多克隆抗体 Polyclonal Antibody to Macrophage Scavenger Receptor 1 (MSR1)
PAA698Hu01 巨噬细胞移动抑制因子(MIF)多克隆抗体 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
PAA698Mu01 巨噬细胞移动抑制因子(MIF)多克隆抗体 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
PAA698Ra01 巨噬细胞移动抑制因子(MIF)多克隆抗体 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
PAA698Mu02 巨噬细胞移动抑制因子(MIF)多克隆抗体 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
PAA698Hu02 巨噬细胞移动抑制因子(MIF)多克隆抗体 Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
PAB060Mu01 巨噬细胞炎性蛋白相关蛋白1(MRP1)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein Related Protein 1 (MRP1)
PAB060Ra01 巨噬细胞炎性蛋白相关蛋白1(MRP1)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein Related Protein 1 (MRP1)
PAA550Hu01 巨噬细胞炎性蛋白5(MIP5)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 5 (MIP5)
PAC091Hu01 巨噬细胞炎性蛋白4α(MIP4α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 4 Alpha (MIP4a)
PAC091Hu02 巨噬细胞炎性蛋白4α(MIP4α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 4 Alpha (MIP4a)
PAA096Bo01 巨噬细胞炎性蛋白3β(MIP3β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)
PAA096Ca01 巨噬细胞炎性蛋白3β(MIP3β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)
PAA096Hu01 巨噬细胞炎性蛋白3β(MIP3β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)
PAA096Mu01 巨噬细胞炎性蛋白3β(MIP3β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)
PAA096Po01 巨噬细胞炎性蛋白3β(MIP3β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)
PAA096Ra01 巨噬细胞炎性蛋白3β(MIP3β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)
PAA096Mu02 巨噬细胞炎性蛋白3β(MIP3β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Beta (MIP3b)
PAA095Bo01 巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAA095Ca01 巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAA095Hu01 巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAA095Mu01 巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAA095Po01 巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAA095Ra01 巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAA095Mu02 巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAA095Hu02 白介素12抗体巨噬细胞炎性蛋白3α(MIP3α)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 3 Alpha (MIP3a)
PAC073Ra01 巨噬细胞炎性蛋白1γ(MIP1γ)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Gamma (MIP1g)
PAC073Mu01 巨噬细胞炎性蛋白1γ(MIP1γ)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Gamma (MIP1g)
PAA093Bo01 巨噬细胞炎性蛋白1β(MIP1β)多克隆抗体 Polyclonal Antibody to Macrophage Inflammatory Protein 1 Beta (MIP1b)