干扰素-gamma受体1抗体
『别名』Interferon-gamma receptor; IFNGR1; CD119; FLJ45734; IFNGR; immune interferon receptor 1; antiviral protein, type 2; CD119 antigen; AVP, type 2; interferon-gamma receptor alpha chain; CDw119; IFN-gamma-R1; IFN-gamma receptor 1.
『浓度』干扰素-gamma受体1抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
干扰素-gamma受体1抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
干扰素-gamma受体1抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAC629Hu01 甲硫氨酸亚砜还原酶A(MSRA)多克隆抗体 Polyclonal Antibody to Methionine Sulfoxide Reductase A (MSRA)
PAG469Hu01 蛋氨酸腺苷转移酶Ⅱα(MAT2α)多克隆抗体 Polyclonal Antibody to Methionine Adenosyltransferase II Alpha (MAT2a)
PAG469Ra01 蛋氨酸腺苷转移酶Ⅱα(MAT2α)多克隆抗体 Polyclonal Antibody to Methionine Adenosyltransferase II Alpha (MAT2a)
PAG470Mu01 蛋氨酸腺苷转移酶Ⅰα(MAT1α)多克隆抗体 Polyclonal Antibody to Methionine Adenosyltransferase I Alpha (MAT1a)
PAG470Mu02 蛋氨酸腺苷转移酶Ⅰα(MAT1α)多克隆抗体 Polyclonal Antibody to Methionine Adenosyltransferase I Alpha (MAT1a)
PAG470Ra01 蛋氨酸腺苷转移酶Ⅰα(MAT1α)多克隆抗体 Polyclonal Antibody to Methionine Adenosyltransferase I Alpha (MAT1a)
PAG466Ge01 蛋氨酸(Met)多克隆抗体 Polyclonal Antibody to Methionine (Met)
PAH662Hu01 镍纹蛋白(METRN)多克隆抗体 Polyclonal Antibody to Meteorin (METRN)
PAH662Mu01 镍纹蛋白(METRN)多克隆抗体 Polyclonal Antibody to Meteorin (METRN)
PAH662Mu02 镍纹蛋白(METRN)多克隆抗体 Polyclonal Antibody to Meteorin (METRN)
PAH662Ra01 镍纹蛋白(METRN)多克隆抗体 Polyclonal Antibody to Meteorin (METRN)
PAF598Hu01 转移关联蛋白2(MTA2)多克隆抗体 Polyclonal Antibody to Metastasis Associated Protein 2 (MTA2)
PAF598Ra01 转移关联蛋白2(MTA2)多克隆抗体 Polyclonal Antibody to Metastasis Associated Protein 2 (MTA2)
PAC630Mu01 转移关联蛋白1(MTA1)多克隆抗体 Polyclonal Antibody to Metastasis Associated Protein 1 (MTA1)
PAM667Hu01 结肠癌转移关联基因1(MACC1)多克隆抗体 Polyclonal Antibody to Metastasis Associated In Colon Cancer 1 (MACC1)
PAA743Hu01 金属硫蛋白3(MT3)多克隆抗体 Polyclonal Antibody to Metallothionein 3 (MT3)
PAA743Mu01 金属硫蛋白3(MT3)多克隆抗体 Polyclonal Antibody to Metallothionein 3 (MT3)
PAA743Mu02 金属硫蛋白3(MT3)多克隆抗体 Polyclonal Antibody to Metallothionein 3 (MT3)
PAB868Hu01 金属硫蛋白2(MT2)多克隆抗体 Polyclonal Antibody to Metallothionein 2 (MT2)
PAB868Mu01 金属硫蛋白2(MT2)多克隆抗体 Polyclonal Antibody to Metallothionein 2 (MT2)
PAB868Ra01 金属硫蛋白2(MT2)多克隆抗体 Polyclonal Antibody to Metallothionein 2 (MT2)
PAB868Rb52 金属硫蛋白2(MT2)多克隆抗体 Polyclonal Antibody to Metallothionein 2 (MT2)
PAB868Rb51 金属硫蛋白2(MT2)多克隆抗体 Polyclonal Antibody to Metallothionein 2 (MT2)
PAB868Mu02 金属硫蛋白2(MT2)多克隆抗体 Polyclonal Antibody to Metallothionein 2 (MT2)
PAD792Hu01 金属硫蛋白1M(MT1M)多克隆抗体 Polyclonal Antibody to Metallothionein 1M (MT1M)
PAD794Hu01 金属硫蛋白1E(MT1E)多克隆抗体 Polyclonal Antibody to Metallothionein 1E (MT1E)
PAB119Hu01 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAB119Mu01 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAB119Ra01 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAB119Rb52 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAB119Ga01 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAB119Rb51 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAB119Mu02 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAB119Ra02 金属硫蛋白1(MT1)多克隆抗体 Polyclonal Antibody to Metallothionein 1 (MT1)
PAC631Hu01 异粘蛋白(MTDH)多克隆抗体 Polyclonal Antibody to Metadherin (MTDH)
PAH615Hu01 间皮素(MSLN)多克隆抗体 Polyclonal Antibody to Mesothelin (MSLN)
PAH615Mu01 间皮素(MSLN)多克隆抗体 Polyclonal Antibody to Mesothelin (MSLN)
PAH615Mu02 间皮素(MSLN)多克隆抗体 Polyclonal Antibody to Mesothelin (MSLN)
PAC300Hu01 中脑星形胶质细胞来源神经营养因子(MANF)多克隆抗体 Polyclonal Antibody to Mesencephalic Astrocyte Derived Neurotrophic Factor (MANF)
PAC625Hu01 巯基丙酮酸硫转移酶(MPST)多克隆抗体 Polyclonal Antibody to Mercaptopyruvate Sulfurtransferase (MPST)
PAC625Mu01 巯基丙酮酸硫转移酶(MPST)多克隆抗体 Polyclonal Antibody to Mercaptopyruvate Sulfurtransferase (MPST)
PAC625Mu02 巯基丙酮酸硫转移酶(MPST)多克隆抗体 Polyclonal Antibody to Mercaptopyruvate Sulfurtransferase (MPST)
PAA173Hu01 甲基多巴1β(MEP1β)多克隆抗体 Polyclonal Antibody to Meprin A Beta (MEP1b)
PAA173Ra01 甲基多巴1β(MEP1β)多克隆抗体 Polyclonal Antibody to Meprin A Beta (MEP1b)
PAA171Hu01 甲基多巴1α(MEP1α)多克隆抗体 Polyclonal Antibody to Meprin A Alpha (MEP1a)
PAA171Mu01 甲基多巴1α(MEP1α)多克隆抗体 Polyclonal Antibody to Meprin A Alpha (MEP1a)
PAA171Mu02 甲基多巴1α(MEP1α)多克隆抗体 Polyclonal Antibody to Meprin A Alpha (MEP1a)
PAB148Hu01 跨膜4域亚家族A成员1(MS4A1)多克隆抗体 Polyclonal Antibody to Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1)
PAB148Mu01 跨膜4域亚家族A成员1(MS4A1)多克隆抗体 Polyclonal Antibody to Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1)
PAA700Hu01 棕榈酰化膜蛋白6(MPP6)多克隆抗体 Polyclonal Antibody to Membrane Protein, Palmitoylated 6 (MPP6)
PAA744Hu01 棕榈酰化膜蛋白5(MPP5)多克隆抗体 Polyclonal Antibody to Membrane Protein, Palmitoylated 5 (MPP5)
PAA680Hu01 棕榈酰化膜蛋白2(MPP2)多克隆抗体 Polyclonal Antibody to Membrane Protein, Palmitoylated 2 (MPP2)
PAA680Ra01 棕榈酰化膜蛋白2(MPP2)多克隆抗体 Polyclonal Antibody to Membrane Protein, Palmitoylated 2 (MPP2)
PAA680Mu01 棕榈酰化膜蛋白2(MPP2)多克隆抗体 Polyclonal Antibody to Membrane Protein, Palmitoylated 2 (MPP2)
PAB275Hu01 膜辅蛋白干扰素-gamma受体1抗体(MCP)多克隆抗体 Polyclonal Antibody to Membrane Cofactor Protein (MCP)
PAB275Ra01 膜辅蛋白(MCP)多克隆抗体 Polyclonal Antibody to Membrane Cofactor Protein (MCP)
PAB275Mu01 膜辅蛋白(MCP)多克隆抗体 Polyclonal Antibody to Membrane Cofactor Protein (MCP)
PAD534Hu01 褪黑素受体1B(MTNR1B)多克隆抗体 Polyclonal Antibody to Melatonin Receptor 1B (MTNR1B)