胰岛素样生长因子结合蛋白3抗体
『别名』IGF BP3; IGF-BP3; Acid stable subunit of the 140 K IGF complex; Binding protein 29; Binding protein 53; BP 53; BP53; Growth hormone dependent binding protein; IBP 3; IBP3; IGF binding protein 3; IGFBP 3;IGFBP-3; Insulin Like Growth Factor Binding Protein 3.
『浓度』胰岛素样生长因子结合蛋白3抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
胰岛素样生长因子结合蛋白3抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
胰岛素样生长因子结合蛋白3抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAP861Hu01 粘蛋白13(MUC13)多克隆抗体 Polyclonal Antibody to Mucin 13, Cell Surface Associated (MUC13)
PAP862Hu01 粘蛋白12(MUC12)多克隆抗体 Polyclonal Antibody to Mucin 12, Cell Surface Associated (MUC12)
PAA413Hu01 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Mu01 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Ra01 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Bo01 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Hu02 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Hu03 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Mu02 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Mu03 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAA413Ra02 粘蛋白1(MUC1)多克隆抗体 Polyclonal Antibody to Mucin 1 (MUC1)
PAS095Hu01 粘蛋白(MUC)多克隆抗体 Polyclonal Antibody to Mucin (MUC)
PAS095Po01 粘蛋白(MUC)多克隆抗体 Polyclonal Antibody to Mucin (MUC)
PAS095Bo01 粘蛋白(MUC)多克隆抗体 Polyclonal Antibody to Mucin (MUC)
PAB097Hu01 运动因子相关蛋白1(MRP1)多克隆抗体 Polyclonal Antibody to Motility Related Protein (MRP1)
PAA575Hu01 胃动素(MTL)多克隆抗体 Polyclonal Antibody to Motilin (MTL)
PAA575Ra01 胃动素(MTL)多克隆抗体 Polyclonal Antibody to Motilin (MTL)
PAA575Rb51 胃动素(MTL)多克隆抗体 Polyclonal Antibody to Motilin (MTL)
PAA575Bo01 胃动素(MTL)多克隆抗体 Polyclonal Antibody to Motilin (MTL)
PAA575Hu02 胃动素(MTL)多克隆抗体 Polyclonal Antibody to Motilin (MTL)
PAA575Po01 胃动素(MTL)多克隆抗体 Polyclonal Antibody to Motilin (MTL)
PAC121Hu01 Smad同源物9(Smad9)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 9 (Smad9)
PAA648Hu01 Smad同源物7(Smad7)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 7 (Smad7)
PAA648Mu01 Smad同源物7(Smad7)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 7 (Smad7)
PAA648Ra01 Smad同源物7(Smad7)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 7 (Smad7)
PAC120Hu01 Smad同源物6(Smad6)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 6 (Smad6)
PAC120Mu01 Smad同源物6(Smad6)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 6 (Smad6)
PAC120Ra01 Smad同源物6(Smad6)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 6 (Smad6)
PAC122Hu01 Smad同源物5(Smad5)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 5 (Smad5)
PAB925Hu01 Smad同源物4(Smad4)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)
PAB925Mu01 Smad同源物4(Smad4)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 4 (Smad4)
PAC123Hu01 Smad同源物3(Smad3)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)
PAC123Ra01 Smad同源物3(Smad3)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)
PAC123Mu01 Smad同源物3(Smad3)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)
PAC123Mu02 Smad同源物3(Smad3)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 3 (Smad3)
PAC124Mu01 Smad同源物2(Smad2)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)
PAC124Hu01 Smad同源物2(Smad2)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)
PAC124Ra01 Smad同源物2(Smad2)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)
PAC124Mu02 Smad同源物2(Smad2)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 2 (Smad2)
PAA647Hu01 Smad同源物1(Smad1)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
PAA647Ra01 Smad同源物1(Smad1)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
PAA647Bo01 Smad同源物1(Smad1)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
PAA647Mu01 Smad同源物1(Smad1)多克隆抗体 Polyclonal Antibody to Mothers Against Decapentaplegic Homolog 1 (Smad1)
PAB928Hu01 干扰素γ诱导单核因子(MIγ)多克隆抗体 Polyclonal Antibody to Monokine Induced By Interferon Gamma (MIg)
PAB928Mu01 干扰素γ诱导单核因子(MIγ)多克隆抗体 Polyclonal Antibody to Monokine Induced By Interferon Gamma (MIg)
PAB928Ra01 干扰素γ诱导单核因子(MIγ)多克隆抗体 Polyclonal Antibody to Monokine Induced By Interferon Gamma (MIg)
PAB928Mu02 干扰素γ诱导单核因子(MIγ)多克隆抗体 Polyclonal Antibody to Monokine Induced By Interferon Gamma (MIg)
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PAB584Hu01 单核细胞巨噬细胞分化关联蛋白(MMA)多克隆抗体 Polyclonal Antibody to Monocyte To Macrophage Differentiation Associated Protein (MMA)
PAC075Mu01 单核细胞趋化蛋白5(MCP5)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 5 (MCP5)
PAC075Mu02 单核细胞趋化蛋白5(MCP5)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 5 (MCP5)
PAA216Ca01 单核细胞趋化蛋白4(MCP4)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 4 (MCP4)
PAA216Eq01 单核细胞趋化蛋白4(MCP4)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 4 (MCP4)
PAA216Hu01 单核细胞趋化蛋白4(MCP4)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 4 (MCP4)
PAA216Hu51 单核细胞趋化蛋白4(MCP4)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 4 (MCP4)
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PAA089Mu01 单核细胞趋化蛋白3(MCP3)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 3 (MCP3)
PAA089Ra01 单核细胞趋化蛋白3(MCP3)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 3 (MCP3)
PAA089Mu02 单核细胞趋化蛋白3(MCP3)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 3 胰岛素样生长因子结合蛋白3抗体(MCP3)
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PAA088Ca01 单核细胞趋化蛋白2(MCP2)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2)
PAA088Eq01 单核细胞趋化蛋白2(MCP2)多克隆抗体 Polyclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2)