白介素2抗体(人)
『别名』Interleukin-2; T-cell growth factor; Aldesleukin; IL 2; Interleukin 2; Interleukin2; Lymphokine; POIL2; T Cell Growth Factor; TCGF.
『浓度』白介素2抗体(人)1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素2抗体(人)洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素2抗体(人)其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAD437Hu01 肌球蛋白轻链12B(MYL12B)多克隆抗体 Polyclonal Antibody to Myosin Light Chain 12B (MYL12B)
PAQ547Hu01 肌球蛋白轻链12A(MYL12A)多克隆抗体 Polyclonal Antibody to Myosin Light Chain 12A (MYL12A)
PAB105Hu01 肌球蛋白轻链1(MYL1)多克隆抗体 Polyclonal Antibody to Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1)
PAB105Ra01 肌球蛋白轻链1(MYL1)多克隆抗体 Polyclonal Antibody to Myosin Light Chain 1, Alkali, Fast Skeletal (MYL1)
PAD436Hu01 肌球蛋白ⅠG(MYO1G)多克隆抗体 Polyclonal Antibody to Myosin IG (MYO1G)
PAD436Mu01 肌球蛋白ⅠG(MYO1G)多克隆抗体 Polyclonal Antibody to Myosin IG (MYO1G)
PAD436Ra01 肌球蛋白ⅠG(MYO1G)多克隆抗体 Polyclonal Antibody to Myosin IG (MYO1G)
PAD435Hu01 肌球蛋白ⅠF(MYO1F)多克隆抗体 Polyclonal Antibody to Myosin IF (MYO1F)
PAD435Mu01 肌球蛋白ⅠF(MYO1F)多克隆抗体 Polyclonal Antibody to Myosin IF (MYO1F)
PAD435Ra01 肌球蛋白ⅠF(MYO1F)多克隆抗体 Polyclonal Antibody to Myosin IF (MYO1F)
PAD434Hu01 肌球蛋白ⅠE(MYO1E)多克隆抗体 Polyclonal Antibody to Myosin IE (MYO1E)
PAD434Mu01 肌球蛋白ⅠE(MYO1E)多克隆抗体 Polyclonal Antibody to Myosin IE (MYO1E)
PAD434Ra01 肌球蛋白ⅠE(MYO1E)多克隆抗体 Polyclonal Antibody to Myosin IE (MYO1E)
PAD433Hu01 肌球蛋白ⅠD(MYO1D)多克隆抗体 Polyclonal Antibody to Myosin ID (MYO1D)
PAD433Mu01 肌球蛋白ⅠD(MYO1D)多克隆抗体 Polyclonal Antibody to Myosin ID (MYO1D)
PAD433Ra01 肌球蛋白ⅠD(MYO1D)多克隆抗体 Polyclonal Antibody to Myosin ID (MYO1D)
PAD432Hu01 肌球蛋白ⅠC(MYO1C)多克隆抗体 Polyclonal Antibody to Myosin IC (MYO1C)
PAD432Mu01 肌球蛋白ⅠC(MYO1C)多克隆抗体 Polyclonal Antibody to Myosin IC (MYO1C)
PAD432Ra01 肌球蛋白ⅠC(MYO1C)多克隆抗体 Polyclonal Antibody to Myosin IC (MYO1C)
PAD431Hu01 肌球蛋白ⅠB(MYO1B)多克隆抗体 Polyclonal Antibody to Myosin IB (MYO1B)
PAD431Mu01 肌球蛋白ⅠB(MYO1B)多克隆抗体 Polyclonal Antibody to Myosin IB (MYO1B)
PAD431Ra01 肌球蛋白ⅠB(MYO1B)多克隆抗体 Polyclonal Antibody to Myosin IB (MYO1B)
PAA375Hu01 肌球蛋白ⅠA(MYO1A)多克隆抗体 Polyclonal Antibody to Myosin IA (MYO1A)
PAA375Mu01 肌球蛋白ⅠA(MYO1A)多克隆抗体 Polyclonal Antibody to Myosin IA (MYO1A)
PAA375Ra01 肌球蛋白ⅠA(MYO1A)多克隆抗体 Polyclonal Antibody to Myosin IA (MYO1A)
PAD422Hu01 肌球蛋白重链9(MYH9)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 9, Non Muscle (MYH9)
PAD421Ra01 肌球蛋白重链8(MYH8)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8)
PAD421Hu01 肌球蛋白重链8(MYH8)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8)
PAP164Hu01 肌球蛋白重链7B(MYH7B)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 7B, Cardiac Muscle Beta (MYH7B)
PAD418Ra01 肌球蛋白重链7(MYH7)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)
PAD418Hu01 肌球蛋白重链7(MYH7)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)
PAD418Mu01 肌球蛋白重链7(MYH7)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)
PAD418Mu02 肌球蛋白重链7(MYH7)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 7, Cardiac Muscle, Beta (MYH7)
PAD419Ra01 肌球蛋白重链6(MYH6)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
PAD419Ra02 肌球蛋白重链6(MYH6)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
PAD419Ra03 肌球蛋白重链6(MYH6)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
PAD419Hu01 肌球蛋白重链6(MYH6)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 6, Cardiac Muscle, Alpha (MYH6)
PAD416Mu01 肌球蛋白重链4(MYH4)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 4, Skeletal Muscle (MYH4)
PAD416Hu01 肌球蛋白重链4(MYH4)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 4, Skeletal Muscle (MYH4)
PAD416Mu02 肌球蛋白重链4(MYH4)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 4, Skeletal Muscle (MYH4)
PAD415Ra01 肌球蛋白重链3(MYH3)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3)
PAD415Hu01 肌球蛋白重链3(MYH3)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 3, Skeletal Muscle, Embryonic (MYH3)
PAA755Mu01 肌球蛋白重链2(MYH2)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)
PAA755Hu01 肌球蛋白重链2(MYH2)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)
PAA755Mu02 肌球蛋白重链2(MYH2)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)
PAD455Hu01 肌球蛋白重链16(MYH16)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 16 (MYH16)
PAD454Hu01 肌球蛋白重链14(MYH14)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 14, Non Muscle (MYH14)
PAD420Ra01 肌球蛋白重链11(MYH11)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 11, Smooth Muscle (MYH11)
PAD420Hu01 肌球蛋白重链11(MYH11)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 11, Smooth Muscle (MYH11)
PAD423Hu01 肌球蛋白重链10(MYH10)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 10, Non Muscle (MYH10)
PAA754Hu01 肌球蛋白重链1(MYH1)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)
PAA754Mu01 肌球蛋白重链1(MYH1)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)
PAA754Mu02 肌球蛋白重链1(MYH1)多克隆抗体 Polyclonal Antibody to Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)
PAD413Mu01 慢型肌球蛋白结合蛋白C(MYBPC1)多克隆抗体 Polyclonal Antibody to Myosin Binding Protein C, Slow Type (MYBPC1)
PAD413Mu02 慢型肌球蛋白结合蛋白C(MYBPC1)多克隆抗体 Polyclonal Antibody to Myosin Binding Protein C, Slow Type (MYBPC1)
PAD016Hu01 心脏肌球蛋白结合蛋白C白介素2抗体(人)(MYBPC3)多克隆抗体 Polyclonal Antibody to Myosin Binding Protein C, Cardiac (MYBPC3)
PAG685Hu01 肌钯蛋白(MYPN)多克隆抗体 Polyclonal Antibody to Myopalladin (MYPN)
PAH585Hu01 肌间蛋白2(MYOM2)多克隆抗体 Polyclonal Antibody to Myomesin 2 (MYOM2)
PAA480Hu01 肌红蛋白(MYO)多克隆抗体 Polyclonal Antibody to Myoglobin (MYO)
PAA480Ra01 肌红蛋白(MYO)多克隆抗体 Polyclonal Antibody to Myoglobin (MYO)