?白介素6
『别名』Interleukin BSF 2; B cell differentiation factor; B cell stimulatory factor 2; BSF 2; BSF2; CDF; CTL differentiation factor; Cytotoxic T cell differentiation factor; Hepatocyte stimulating factor; HGF; HPGF; HSF; Hybridoma growth factor; Hybridoma plasmacytoma growth factor; IFNB2; IL 6; IL6 protein; Interferon beta 2; Interleukin 6 (interferon beta 2); Interleukin 6; Interleukin6; IL6_HUMAN.
『浓度』白介素61mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素6洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素6其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA105Mu02 神经生长因子(NGF)多克隆抗体 Polyclonal Antibody to Nerve Growth Factor (NGF)
PAB785Hu01 脑啡肽酶(NEP)多克隆抗体 Polyclonal Antibody to Neprilysin (NEP)
PAB785Ra01 脑啡肽酶(NEP)多克隆抗体 Polyclonal Antibody to Neprilysin (NEP)
PAB785Hu02 脑啡肽酶(NEP)多克隆抗体 Polyclonal Antibody to Neprilysin (NEP)
PAB785Ra02 脑啡肽酶(NEP)多克隆抗体 Polyclonal Antibody to Neprilysin (NEP)
PAB785Mu01 脑啡肽酶(NEP)多克隆抗体 Polyclonal Antibody to Neprilysin (NEP)
PAB785Mu02 脑啡肽酶(NEP)多克隆抗体 Polyclonal Antibody to Neprilysin (NEP)
PAH522Hu01 肾连蛋白(NPNT)多克隆抗体 Polyclonal Antibody to Nephronectin (NPNT)
PAH522Mu01 肾连蛋白(NPNT)多克隆抗体 Polyclonal Antibody to Nephronectin (NPNT)
PAH522Hu02 肾连蛋白(NPNT)多克隆抗体 Polyclonal Antibody to Nephronectin (NPNT)
PAH522Mu02 肾连蛋白(NPNT)多克隆抗体 Polyclonal Antibody to Nephronectin (NPNT)
PAG309Mu01 肾母细胞瘤过表达基因(NOV)多克隆抗体 Polyclonal Antibody to Nephroblastoma Overexpressed Gene (NOV)
PAG309Ra01 肾母细胞瘤过表达基因(NOV)多克隆抗体 Polyclonal Antibody to Nephroblastoma Overexpressed Gene (NOV)
PAA937Hu01 肾足蛋白(NPHN)多克隆抗体 Polyclonal Antibody to Nephrin (NPHN)
PAA937Mu01 肾足蛋白(NPHN)多克隆抗体 Polyclonal Antibody to Nephrin (NPHN)
PAA937Ra01 肾足蛋白(NPHN)多克隆抗体 Polyclonal Antibody to Nephrin (NPHN)
PAA937Mu02 肾足蛋白(NPHN)多克隆抗体 Polyclonal Antibody to Nephrin (NPHN)
PAA937Hu02 肾足蛋白(NPHN)多克隆抗体 Polyclonal Antibody to Nephrin (NPHN)
PAL935Hu01 再生蛋白1(NEO1)多克隆抗体 Polyclonal Antibody to Neogenin 1 (NEO1)
PAL934Hu01 NEL样蛋白2(NELL2)多克隆抗体 Polyclonal Antibody to NEL Like Protein 2 (NELL2)
PAN291Mu01 Nei内切核酸酶Ⅷ样蛋白3(NEIL3)多克隆抗体 Polyclonal Antibody to Nei Endonuclease VIII Like Protein 3 (NEIL3)
PAN291Mu02 Nei内切核酸酶Ⅷ样蛋白3(NEIL3)多克隆抗体 Polyclonal Antibody to Nei Endonuclease VIII Like Protein 3 (NEIL3)
PAL915Hu01 Nei内切核酸酶Ⅷ样蛋白1(NEIL1)多克隆抗体 Polyclonal Antibody to Nei Endonuclease VIII Like Protein 1 (NEIL1)
PAJ541Hu01 Necdin蛋白(NDN)多克隆抗体 Polyclonal Antibody to Necdin (NDN)
PAB725Ra01 自然杀伤细胞受体2B4(NKR2B4)多克隆抗体 Polyclonal Antibody to Natural Killer Cell Receptor 2B4 (NKR2B4)
PAB725Mu01 自然杀伤细胞受体2B4(NKR2B4)多克隆抗体 Polyclonal Antibody to Natural Killer Cell Receptor 2B4 (NKR2B4)
PAD046Hu01 利钠肽受体3(NPR3)多克隆抗体 Polyclonal Antibody to Natriuretic Peptide Receptor 3 (NPR3)
PAD046Ra01 利钠肽受体3(NPR3)多克隆抗体 Polyclonal Antibody to Natriuretic Peptide Receptor 3 (NPR3)
PAD045Hu01 利钠肽受体2(NPR2)多克隆抗体 Polyclonal Antibody to Natriuretic Peptide Receptor 2 (NPR2)
PAD045Ra01 利钠肽受体2(NPR2)多克隆抗体 Polyclonal Antibody to Natriuretic Peptide Receptor 2 (NPR2)
PAC665Hu01 利钠肽受体1(NPR1)多克隆抗体 Polyclonal Antibody to Natriuretic Peptide Receptor 1 (NPR1)
PAC665Ra01 利钠肽受体1(NPR1)多克隆抗体 Polyclonal Antibody to Natriuretic Peptide Receptor 1 (NPR1)
PAK521Ge01 纳他霉素(NTM)多克隆抗体 Polyclonal Antibody to Natamycin (NTM)
PAC650Hu01 Napsin A天冬氨酸肽酶(NAPSA)多克隆抗体 Polyclonal Antibody to Napsin A Aspartic Peptidase (NAPSA)
PAE359Hu01 钠钾氯协同转运蛋白2(NKCC2)多克隆抗体 Polyclonal Antibody to Na-K-Cl Cotransporter 2 (NKCC2)
PAL969Hu01 醌NADH脱氢酶1(NQO1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)
PAL969Mu01 醌NADH脱氢酶1(NQO1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)
PAL969Ra01 醌NADH脱氢酶1(NQO1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)
PAL969Mu02 醌NADH脱氢酶1(NQO1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase, Quinone 1 (NQO1)
PAJ794Hu01 泛醌NADH脱氢酶Fe-S蛋白1(NDUFS1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase Ubiquinone Fe-S Protein 1 (NDUFS1)
PAS952Hu01 NADH脱氢酶5(ND5)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase 5 (ND5)
PAS952Ra01 NADH脱氢酶5(ND5)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase 5 (ND5)
PAQ301Mu01 NADH脱氢酶1(ND1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase 1 (ND1)
PAQ301Mu02 NADH脱氢酶1(ND1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase 1 (ND1)
PAQ301Ra01 NADH脱氢酶1(ND1)多克隆抗体 Polyclonal Antibody to NADH Dehydrogenase 1 (ND1)
PAH578Hu01 NAD激酶(NADK)多克隆抗体 Polyclonal Antibody to NAD Kinase (NADK)
PAE689Hu01 N-酰基鞘氨醇酰胺水解酶2(ASAH2)多克隆抗体 Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 2 (ASAH2)
PAC302Hu01 N-酰基鞘氨醇酰胺水解酶1(ASAH1)多克隆抗体 Polyclonal Antibody to N-Acylsphingosine Amidohydrolase 1 (ASAH1)
PAE690Hu01 N-酰基乙醇氨酸酰化酶(NAAA)多克隆抗体 Polyclonal Antibody to N-Acylethanolamine Acid Amidase (NAAA)
PAE361Hu01 钠氯协同转运蛋白(NCCT)多克隆抗体 Polyclonal Antibody to Na-Cl Cotransporter (NCCT)
PAE361Hu02 钠氯协同转运蛋白(NCCT)多克隆抗体 Polyclonal Antibody to Na-Cl Cotransporter (NCCT)
PAN064Hu01 N-乙酰转移酶8样蛋白(NAT8L)多克隆抗体 Polyclonal Antibody to N-Acetyltransferase 8 Like Protein (NAT8L)
PAG192Ra01 N-乙酰转移酶2(NAT2)多克隆抗体 Polyclonal Antibody to N-Acetyltransferase 2 (NAT2)
PAG191Hu01 N-乙酰转移酶1白介素6(NAT1)多克隆抗体 Polyclonal Antibody to N-Acetyltransferase 1 (NAT1)
PAG191Mu01 N-乙酰转移酶1(NAT1)多克隆抗体 Polyclonal Antibody to N-Acetyltransferase 1 (NAT1)
PAG191Mu02 N-乙酰转移酶1(NAT1)多克隆抗体 Polyclonal Antibody to N-Acetyltransferase 1 (NAT1)
PAH568Hu01 N-乙酰神经氨酰酸合酶(NANS)多克隆抗体 Polyclonal Antibody to N-Acetylneuraminic Acid Synthase (NANS)