抑制素βC抗体
『别名』ACTIVIN BETA C; IHBC; INHBC; MGC108687; activin beta-C chain; Inhibin, beta C; inhibin beta C chain precursor; Inhbc; INHBC_HUMAN.
『浓度』抑制素βC抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
抑制素βC抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
抑制素βC抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAH547Hu01 核因子相关κB结合蛋白(NFRkB)多克隆抗体 Polyclonal Antibody to Nuclear Factor Related To Kappa B Binding Protein (NFRkB)
PAL942Hu01 活化T-细胞核因子2(NFATC2)多克隆抗体 Polyclonal Antibody to Nuclear Factor Of Activated T-Cells, Cytoplasmic 2 (NFATC2)
PAL941Mu01 激活T-细胞核因子1(NFATC1)多克隆抗体 Polyclonal Antibody to Nuclear Factor Of Activated T-Cells, Cytoplasmic 1 (NFATC1)
PAL941Mu02 激活T-细胞核因子1(NFATC1)多克隆抗体 Polyclonal Antibody to Nuclear Factor Of Activated T-Cells, Cytoplasmic 1 (NFATC1)
PAB825Mu01 核因子κB2(NFκB2)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B2 (NFkB2)
PAB825Hu01 核因子κB2(NFκB2)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B2 (NFkB2)
PAB825Mu02 核因子κB2(NFκB2)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B2 (NFkB2)
PAB825Ga01 核因子κB2(NFκB2)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B2 (NFkB2)
PAB824Ga01 核因子κB(NFκB)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)
PAB824Hu01 核因子κB(NFκB)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)
PAB824Mu01 核因子κB(NFκB)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)
PAB824Ra01 核因子κB(NFκB)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)
PAB824Hu02 核因子κB(NFκB)多克隆抗体 Polyclonal Antibody to Nuclear Factor Kappa B (NFkB)
PAF665Ra01 核因子I/X(NFIX)多克隆抗体 Polyclonal Antibody to Nuclear Factor I/X (NFIX)
PAF663Mu01 核因子I/B(NFIB)多克隆抗体 Polyclonal Antibody to Nuclear Factor I/B (NFIB)
PAF663Hu01 核因子I/B(NFIB)多克隆抗体 Polyclonal Antibody to Nuclear Factor I/B (NFIB)
PAF663Mu02 核因子I/B(NFIB)多克隆抗体 Polyclonal Antibody to Nuclear Factor I/B (NFIB)
PAJ712Hu01 核分布基因C同源物(NUDC)多克隆抗体 Polyclonal Antibody to Nuclear Distribution Gene C Homolog (NUDC)
PAL975Hu01 NUAK家族SNF1样激酶1(NUAK1)多克隆抗体 Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1)
PAL975Mu01 NUAK家族SNF1样激酶1(NUAK1)多克隆抗体 Polyclonal Antibody to NUAK Family SNF1 Like Kinase 1 (NUAK1)
PAA485Hu01 氨基端前脑钠素(NT-ProBNP)多克隆抗体 Polyclonal Antibody to N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP)
PAA484Hu01 氨基端前心钠肽(NT-ProANP)多克隆抗体 Polyclonal Antibody to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP)
PAA484Mu01 氨基端前心钠肽(NT-ProANP)多克隆抗体 Polyclonal Antibody to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP)
PAA484Ra01 氨基端前心钠肽(NT-ProANP)多克隆抗体 Polyclonal Antibody to N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP)
PAH178Hu01 N-磺氨基葡糖磺基氢化酶(SGSH)多克隆抗体 Polyclonal Antibody to N-Sulfoglucosamine Sulfohydrolase (SGSH)
PAH178Mu01 N-磺氨基葡糖磺基氢化酶(SGSH)多克隆抗体 Polyclonal Antibody to N-Sulfoglucosamine Sulfohydrolase (SGSH)
PAH178Mu02 N-磺氨基葡糖磺基氢化酶(SGSH)多克隆抗体 Polyclonal Antibody to N-Sulfoglucosamine Sulfohydrolase (SGSH)
PAS272Hu01 Notch2氨基端样蛋白(NOTCH2NL)多克隆抗体 Polyclonal Antibody to Notch2 N-Terminal Like Protein (NOTCH2NL)
PAS396Mu01 Notch调控锚蛋白重复蛋白(NRARP)多克隆抗体 Polyclonal Antibody to Notch Regulated Ankyrin Repeat Protein (NRARP)
PAL149Hu01 Notch同源物4(NOTCH4)多克隆抗体 Polyclonal Antibody to Notch Homolog 4 (NOTCH4)
PAL149Ra01 Notch同源物4(NOTCH4)多克隆抗体 Polyclonal Antibody to Notch Homolog 4 (NOTCH4)
PAL147Hu01 Notch同源物3(NOTCH3)多克隆抗体 Polyclonal Antibody to Notch Homolog 3 (NOTCH3)
PAL148Hu01 Notch同源物2(NOTCH2)多克隆抗体 Polyclonal Antibody to Notch Homolog 2 (NOTCH2)
PAM892Hu01 食管特定正常黏膜蛋白1(NMES1)多克隆抗体 Polyclonal Antibody to Normal Mucosa Of Esophagus Specific 1 (NMES1)
PAC681Hu01 去甲肾上腺素转运蛋白(NET)多克隆抗体 Polyclonal Antibody to Norepinephrine Transporter (NET)
PAC681Mu01 去甲肾上腺素转运蛋白(NET)多克隆抗体 Polyclonal Antibody to Norepinephrine Transporter (NET)
PAC681Mu02 去甲肾上腺素转运蛋白(NET)多克隆抗体 Polyclonal Antibody to Norepinephrine Transporter (NET)
PAC681Ra01 去甲肾上腺素转运蛋白(NET)多克隆抗体 Polyclonal Antibody to Norepinephrine Transporter (NET)
PAA907Ge01 去甲肾上腺素(NE)多克隆抗体 Polyclonal Antibody to Noradrenaline (NE)
PAJ818Hu01 含非POU域八聚体结合蛋白(NONO)多克隆抗体 Polyclonal Antibody to Non POU Domain Containing Octamer Binding Protein (NONO)
PAN537Ra01 非转移细胞6表达蛋白(NME6)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 6, Protein Expressed In (NME6)
PAN537Mu01 非转移细胞6表达蛋白(NME6)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 6, Protein Expressed In (NME6)
PAN537Hu01 非转移细胞6表达蛋白(NME6)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 6, Protein Expressed In (NME6)
PAK245Mu01 非转移细胞5表达NM23A蛋白(NME5)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 5, Protein NM23A Expressed In (NME5)
PAK244Mu01 非转移细胞4表达NM23A蛋白(NME4)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 4, Protein NM23A Expressed In (NME4)
PAK244Hu01 非转移细胞4表达NM23A蛋白(NME4)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 4, Protein NM23A Expressed In (NME4)
PAK243Hu01 非转移细胞3表达NM23A蛋白(NME3)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
PAK243Mu01 非转移细胞3表达NM23A蛋白(NME3)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
PAJ816Mu01 非转移细胞2表达NM23A蛋白(NME2)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 2, Protein NM23B Expressed In (NME2)
PAJ817Hu01 非转移细胞1表达NM23A蛋白(NME1)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 1, Protein NM23A Expressed In (NME1)
PAJ817Mu01 非转移细胞1表达NM23A蛋白(NME1)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 1, Protein NM23A Expressed In (NME1)
PAJ817Ra01 非转移细胞1表达NM23A蛋白(NME1)多克隆抗体 Polyclonal Antibody to Non Metastatic Cells 1, Protein NM23A Expressed In (NME1)
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PAC130Hu01 头蛋白(NOG)多克隆抗体 Polyclonal Antibody to Noggin (NOG)
PAC130Mu01 头蛋白抑制素βC抗体(NOG)多克隆抗体 Polyclonal Antibody to Noggin (NOG)
PAC130Ra01 头蛋白(NOG)多克隆抗体 Polyclonal Antibody to Noggin (NOG)
PAC130Ca01 头蛋白(NOG)多克隆抗体 Polyclonal Antibody to Noggin (NOG)
PAC130Ga01 头蛋白(NOG)多克隆抗体 Polyclonal Antibody to Noggin (NOG)
PAC130Po01 头蛋白(NOG)多克隆抗体 Polyclonal Antibody to Noggin (NOG)
PAC130Hu02 头蛋白(NOG)多克隆抗体 Polyclonal Antibody to Noggin (NOG)