白介素-22受体抗体
『别名』Interleukin-22 receptor subunit alpha-1; IL-22RA1; zcytoR11; IL-22R-alpha-1; IL-22 receptor subunit alpha-1; cytokine receptor class-II member 9; cytokine receptor family 2 member 9; IL22RA1; CRF2-9; IL22R; IL22R1; I22R1_HUMAN.
『浓度』白介素-22受体抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素-22受体抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素-22受体抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAC704Mu01 8-羟基鸟嘌呤糖苷酶1(OGG1)多克隆抗体 Polyclonal Antibody to Oxoguanine Glycosylase 1 (OGG1)
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PAG187Hu01 酮戊二酸脱氢酶(OGDH)多克隆抗体 Polyclonal Antibody to Oxoglutarate Dehydrogenase (OGDH)
PAG187Ra01 酮戊二酸脱氢酶(OGDH)多克隆抗体 Polyclonal Antibody to Oxoglutarate Dehydrogenase (OGDH)
PAB898Hu01 氧鲨烯环化酶(OSC)多克隆抗体 Polyclonal Antibody to Oxidosqualene Cyclase (OSC)
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PAG202Hu01 氧化应激诱导生长抑制因子1(OSGIN1)多克隆抗体 Polyclonal Antibody to Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1)
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PAL838Hu01 卵巢肿瘤蛋白1(OTUB1)多克隆抗体 Polyclonal Antibody to Otubain 1 (OTUB1)
PAL838Mu01 卵巢肿瘤蛋白1(OTUB1)多克隆抗体 Polyclonal Antibody to Otubain 1 (OTUB1)
PAL838Ra01 卵巢肿瘤蛋白1(OTUB1)多克隆抗体 Polyclonal Antibody to Otubain 1 (OTUB1)
PAN863Mu01 Osterix蛋白(OSX)多克隆抗体 Polyclonal Antibody to Osterix (OSX)
PAN863Hu01 Osterix蛋白(OSX)多克隆抗体 Polyclonal Antibody to Osterix (OSX)
PAN863Mu02 Osterix蛋白(OSX)多克隆抗体 Polyclonal Antibody to Osterix (OSX)
PAA108Cp01 骨保护素(OPG)多克隆抗体 Polyclonal Antibody to Osteoprotegerin (OPG)
PAA108Ga01 骨保护素(OPG)多克隆抗体 Polyclonal Antibody to Osteoprotegerin (OPG)
PAA108Hu01 骨保护素(OPG)多克隆抗体 Polyclonal Antibody to Osteoprotegerin (OPG)
PAA108Mu01 骨保护素(OPG)多克隆抗体 Polyclonal Antibody to Osteoprotegerin (OPG)
PAA108Po01 骨保护素(OPG)多克隆抗体 Polyclonal Antibody to Osteoprotegerin (OPG)
PAA108Ra01 骨保护素(OPG)多克隆抗体 Polyclonal Antibody to Osteoprotegerin (OPG)
PAA108Mu02 骨保护素(OPG)多克隆抗体 Polyclonal Antibody to Osteoprotegerin (OPG)
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PAA899Mu01 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA899Po01 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA899Ra01 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA899Rb51 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA899Bo01 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA899Mu02 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA899Si01 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA899Hu02 骨桥素(OPN)多克隆抗体 Polyclonal Antibody to Osteopontin (OPN)
PAA791Mu01 骨粘连蛋白(ON)多克隆抗体 Polyclonal Antibody to Osteonectin (ON)
PAA791Ra01 骨粘连蛋白(ON)多克隆抗体 Polyclonal Antibody to Osteonectin (ON)
PAA791Mu02 骨粘连蛋白(ON)多克隆抗体 Polyclonal Antibody to Osteonectin (ON)
PAA791Hu01 骨粘连蛋白(ON)多克隆抗体 Polyclonal Antibody to Osteonectin (ON)
PAA791Rb51 骨粘连蛋白(ON)多克隆抗体 Polyclonal Antibody to Osteonectin (ON)
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PAC688Mu01 骨诱导因子(OGN)多克隆抗体 Polyclonal Antibody to Osteoglycin (OGN)
PAC688Ra01 骨诱导因子(OGN)多克隆抗体 Polyclonal Antibody to Osteoglycin (OGN)
PAC688Mu02 骨诱导因子(OGN)多克隆抗体 Polyclonal Antibody to Osteoglycin (OGN)
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PAC695Hu01 破骨细胞关联受体(OSCAR)多克隆抗体 Polyclonal Antibody to Osteoclast Associated Receptor (OSCAR)
PAA471Bo01 骨钙素(OC)多克隆抗体 Polyclonal Antibody to Osteocalcin (OC)
PAA471Ca01 骨钙素(OC)多克隆抗体 Polyclonal Antibody to Osteocalcin (OC)
PAA471Hu01 骨钙素(OC)多克隆抗体 Polyclonal Antibody to Osteocalcin (OC)
PAA471Mu01 骨钙素白介素-22受体抗体(OC)多克隆抗体 Polyclonal Antibody to Osteocalcin (OC)
PAA471Po01 骨钙素(OC)多克隆抗体 Polyclonal Antibody to Osteocalcin (OC)
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