白介素21受体抗体
『别名』IL 21R; IL21 Receptor; IL21R; Interleukin 21 receptor; MGC10967; NILR; IL21R_HUMAN.
『浓度』白介素21受体抗体1mg/1ml
『规格』0.2ml/100μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素21受体抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素21受体抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAF822Ra01 磷酸酶张力蛋白同源物(PTEN)多克隆抗体 Polyclonal Antibody to Phosphatase And Tensin Homolog (PTEN)
PAC750Hu01 苯丙氨酸羟化酶(PAH)多克隆抗体 Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)
PAC750Hu02 苯丙氨酸羟化酶(PAH)多克隆抗体 Polyclonal Antibody to Phenylalanine Hydroxylase (PAH)
PAS111Ge01 苯丙氨酸(Phe)多克隆抗体 Polyclonal Antibody to Phenylalanine (Phe)
PAB268Hu01 苯酚磺基转移酶(PST)多克隆抗体 Polyclonal Antibody to Phenol Sulfotransferase (PST)
PAB268Mu01 苯酚磺基转移酶(PST)多克隆抗体 Polyclonal Antibody to Phenol Sulfotransferase (PST)
PAB268Ra01 苯酚磺基转移酶(PST)多克隆抗体 Polyclonal Antibody to Phenol Sulfotransferase (PST)
PAB268Mu02 苯酚磺基转移酶(PST)多克隆抗体 Polyclonal Antibody to Phenol Sulfotransferase (PST)
PAH375Mu01 PHD指蛋白8(PHF8)多克隆抗体 Polyclonal Antibody to PHD Finger Protein 8 (PHF8)
PAH375Mu02 PHD指蛋白8(PHF8)多克隆抗体 Polyclonal Antibody to PHD Finger Protein 8 (PHF8)
PAK173Hu01 Pescadillo同源物1(PES1)多克隆抗体 Polyclonal Antibody to Pescadillo Homolog 1 (PES1)
PAC170Hu01 Persephin蛋白(PSPN)多克隆抗体 Polyclonal Antibody to Persephin (PSPN)
PAC170Mu01 Persephin蛋白(PSPN)多克隆抗体 Polyclonal Antibody to Persephin (PSPN)
PAC170Mu02 Persephin蛋白(PSPN)多克隆抗体 Polyclonal Antibody to Persephin (PSPN)
PAH337Hu01 过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)
PAH337Ra01 过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)
PAH337Mu01 过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)
PAH337Mu02 过氧化物酶体增殖物激活受体γ辅激活因子1α(PPARγC1α)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a)
PAA886Bo01 过氧化物酶体增殖物激活受体γ(PPARγ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PAA886Hu01 过氧化物酶体增殖物激活受体γ(PPARγ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PAA886Mu01 过氧化物酶体增殖物激活受体γ(PPARγ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PAA886Ra01 过氧化物酶体增殖物激活受体γ(PPARγ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PAA886Rb51 过氧化物酶体增殖物激活受体γ(PPARγ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PAA886Mu02 过氧化物酶体增殖物激活受体γ(PPARγ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PAA886Hu02 过氧化物酶体增殖物激活受体γ(PPARγ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PAC041Hu01 过氧化物酶体增殖物激活受体δ(PPARδ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Delta (PPARd)
PAC041Mu01 过氧化物酶体增殖物激活受体δ(PPARδ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Delta (PPARd)
PAC041Hu02 过氧化物酶体增殖物激活受体δ(PPARδ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Delta (PPARd)
PAC041Mu02 过氧化物酶体增殖物激活受体δ(PPARδ)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Delta (PPARd)
PAA934Hu01 过氧化物酶体增殖物激活受体α(PPARα)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Alpha (PPARa)
PAA934Mu01 过氧化物酶体增殖物激活受体α(PPARα)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Alpha (PPARa)
PAA934Mu02 过氧化物酶体增殖物激活受体α(PPARα)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Alpha (PPARa)
PAA934Ra01 过氧化物酶体增殖物激活受体α(PPARα)多克隆抗体 Polyclonal Antibody to Peroxisome Proliferator Activated Receptor Alpha (PPARa)
PAA602Hu01 过氧化物酶体生物合成因子2(PEX2)多克隆抗体 Polyclonal Antibody to Peroxisomal Biogenesis Factor 2 (PEX2)
PAA602Mu01 过氧化物酶体生物合成因子2(PEX2)多克隆抗体 Polyclonal Antibody to Peroxisomal Biogenesis Factor 2 (PEX2)
PAA602Ra01 过氧化物酶体生物合成因子2(PEX2)多克隆抗体 Polyclonal Antibody to Peroxisomal Biogenesis Factor 2 (PEX2)
PAA602Hu02 过氧化物酶体生物合成因子2(PEX2)多克隆抗体 Polyclonal Antibody to Peroxisomal Biogenesis Factor 2 (PEX2)
PAF756Hu01 过氧化还原酶6(PRDX6)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 6 (PRDX6)
PAF756Ra01 过氧化还原酶6(PRDX6)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 6 (PRDX6)
PAF756Ra02 过氧化还原酶6(PRDX6)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 6 (PRDX6)
PAF756Mu01 过氧化还原酶6(PRDX6)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 6 (PRDX6)
PAF756Mu02 过氧化还原酶6(PRDX6)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 6 (PRDX6)
PAF756Bo01 过氧化还原酶6(PRDX6)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 6 (PRDX6)
PAF755Hu01 过氧化还原酶5(PRDX5)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 5 (PRDX5)
PAF755Mu01 过氧化还原酶5(PRDX5)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 5 (PRDX5)
PAF755Ra01 过氧化还原酶5(PRDX5)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 5 (PRDX5)
PAF755Mu02 过氧化还原酶5(PRDX5)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 5 (PRDX5)
PAF754Hu01 过氧化还原酶4(PRDX4)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 4 (PRDX4)
PAF754Mu01 过氧化还原酶4(PRDX4)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 4 (PRDX4)
PAF754Mu02 过氧化还原酶4(PRDX4)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 4 (PRDX4)
PAF753Hu01 过氧化还原酶3(PRDX3)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 3 (PRDX3)
PAF753Ra01 过氧化还原酶3(PRDX3)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 3 (PRDX3)
PAF753Mu01 过氧化还原酶3(PRDX3)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 3 (PRDX3)
PAF757Hu01 过氧化还原酶2(PRDX2)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 2 (PRDX2)
PAF757Mu01 过氧化还原酶2(PRDX2)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 2 (PRDX2)
PAF757Ra01 过氧化还原酶2(PRDX2)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 2 (PRDX2)
PAF757Hu02 过氧化还原酶2白介素21受体抗体(PRDX2)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 2 (PRDX2)
PAC749Hu01 过氧化还原酶1(PRDX1)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 1 (PRDX1)
PAC749Mu01 过氧化还原酶1(PRDX1)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 1 (PRDX1)
PAC749Mu02 过氧化还原酶1(PRDX1)多克隆抗体 Polyclonal Antibody to Peroxiredoxin 1 (PRDX1)