白介素21抗体
『别名』IL 21; IL21; Interleukin 21; Interleukin21; Za11.
『浓度』白介素21抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素21抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素21抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA576Hu04 磷脂酶A2激活蛋白(PLAA)多克隆抗体 Polyclonal Antibody to Phospholipase A2 Activating Protein (PLAA)
PAA576Hu03 磷脂酶A2激活蛋白(PLAA)多克隆抗体 Polyclonal Antibody to Phospholipase A2 Activating Protein (PLAA)
PAA576Hu05 磷脂酶A2激活蛋白(PLAA)多克隆抗体 Polyclonal Antibody to Phospholipase A2 Activating Protein (PLAA)
PAB151Hu01 磷脂酶A1(PLA1)多克隆抗体 Polyclonal Antibody to Phospholipase A1 (PLA1)
PAB151Hu02 磷脂酶A1(PLA1)多克隆抗体 Polyclonal Antibody to Phospholipase A1 (PLA1)
PAB884Mu01 受磷蛋白(PLN)多克隆抗体 Polyclonal Antibody to Phospholamban (PLN)
PAB884Mu02 受磷蛋白(PLN)多克隆抗体 Polyclonal Antibody to Phospholamban (PLN)
PAB884Hu01 受磷蛋白(PLN)多克隆抗体 Polyclonal Antibody to Phospholamban (PLN)
PAJ826Ra01 磷酸肌醇-3-激酶2β肽(PI3K2β)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Class-2-Beta Polypeptide (PIK3C2b)
PAA383Hu01 磷酸肌醇-3-激酶2α肽(PI3K2α)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a)
PAA383Mu01 磷酸肌醇-3-激酶2α肽(PI3K2α)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a)
PAA383Mu02 磷酸肌醇-3-激酶2α肽(PI3K2α)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a)
PAJ828Hu01 磷酸肌醇-3-激酶3(PIK3C3)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Class 3 (PIK3C3)
PAJ832Hu01 磷酸肌醇-3-激酶催化亚基δ肽(PIK3Cδ)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd)
PAJ829Hu01 磷酸肌醇-3-激酶催化亚基β肽(PIK3Cβ)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
PAJ829Hu02 磷酸肌醇-3-激酶催化亚基β肽(PIK3Cβ)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
PAJ829Hu03 磷酸肌醇-3-激酶催化亚基β肽(PIK3Cβ)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Catalytic Beta Polypeptide (PIK3Cb)
PAJ836Ra01 磷酸肌醇-3-激酶衔接蛋白1(PIK3AP1)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1)
PAJ836Hu01 磷酸肌醇-3-激酶衔接蛋白1(PIK3AP1)多克隆抗体 Polyclonal Antibody to Phosphoinositide-3-Kinase Adaptor Protein 1 (PIK3AP1)
PAL531Mu01 含FYVE指磷酸肌醇激酶(PIKFYVE)多克隆抗体 Polyclonal Antibody to Phosphoinositide Kinase, FYVE Finger Containing (PIKFYVE)
PAC246Mu01 磷酸肌醇依赖性蛋白激酶1(PDPK1)多克隆抗体 Polyclonal Antibody to Phosphoinositide Dependent Protein Kinase 1 (PDPK1)
PAC246Hu01 磷酸肌醇依赖性蛋白激酶1(PDPK1)多克隆抗体 Polyclonal Antibody to Phosphoinositide Dependent Protein Kinase 1 (PDPK1)
PAC246Mu02 磷酸肌醇依赖性蛋白激酶1(PDPK1)多克隆抗体 Polyclonal Antibody to Phosphoinositide Dependent Protein Kinase 1 (PDPK1)
PAH413Hu01 磷酸组氨酸性磷酸酶1(PHPT1)多克隆抗体 Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)
PAH413Mu01 磷酸组氨酸性磷酸酶1(PHPT1)多克隆抗体 Polyclonal Antibody to Phosphohistidine Phosphatase 1 (PHPT1)
PAE528Hu01 磷酸甘油酸变位酶1(PGAM1)多克隆抗体 Polyclonal Antibody to Phosphoglycerate Mutase 1, Brain (PGAM1)
PAG304Hu01 磷酸甘油酸酯激酶1(PGK1)多克隆抗体 Polyclonal Antibody to Phosphoglycerate Kinase 1 (PGK1)
PAF741Hu01 葡萄糖磷酸变位酶5(PGM5)多克隆抗体 Polyclonal Antibody to Phosphoglucomutase 5 (PGM5)
PAB745Hu01 血小板型磷酸果糖激酶(PFKP)多克隆抗体 Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
PAB745Mu01 血小板型磷酸果糖激酶(PFKP)多克隆抗体 Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
PAB745Ra01 血小板型磷酸果糖激酶(PFKP)多克隆抗体 Polyclonal Antibody to Phosphofructokinase, Platelet (PFKP)
PAD405Hu01 肌肉磷酸果糖激酶(PFKM)多克隆抗体 Polyclonal Antibody to Phosphofructokinase, Muscle (PFKM)
PAD405Ra01 肌肉磷酸果糖激酶(PFKM)多克隆抗体 Polyclonal Antibody to Phosphofructokinase, Muscle (PFKM)
PAD406Ra01 肝脏磷酸果糖激酶(PFKL)多克隆抗体 Polyclonal Antibody to Phosphofructokinase, Liver (PFKL)
PAD583Hu01 磷酸烯醇式丙酮酸羧激酶2(PCK2)多克隆抗体 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 2, Mitochondrial (PCK2)
PAA936Hu01 磷酸烯醇式丙酮酸羧激酶1(PCK1)多克隆抗体 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
PAA936Mu01 磷酸烯醇式丙酮酸羧激酶1(PCK1)多克隆抗体 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
PAA936Mu02 磷酸烯醇式丙酮酸羧激酶1(PCK1)多克隆抗体 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
PAA936Ra01 磷酸烯醇式丙酮酸羧激酶1(PCK1)多克隆抗体 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
PAF645Hu01 磷酸二酯酶5A(PDE5A)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 5A, cGMP Specific (PDE5A)
PAF644Hu01 磷酸二酯酶4D(PDE4D)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 4D, cAMP Specific (PDE4D)
PAF642Ra01 磷酸二酯酶4B(PDE4B)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 4B, cAMP Specific (PDE4B)
PAF639Ra01 磷酸二酯酶3A(PDE3A)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 3A, cGMP Inhibited (PDE3A)
PAF637Mu01 磷酸二酯酶1C(PDE1C)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 1C, Calmodulin Dependent (PDE1C)
PAF637Mu02 磷酸二酯酶1C(PDE1C)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 1C, Calmodulin Dependent (PDE1C)
PAF635Hu01 磷酸二酯酶1A(PDE1A)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 1A, Calmodulin Dependent (PDE1A)
PAF658Mu01 磷酸二酯酶12(PDE12)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 12 (PDE12)
PAF658Mu02 磷酸二酯酶12(PDE12)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 12 (PDE12)
PAF656Hu01 磷酸二酯酶10A(PDE10A)多克隆抗体 Polyclonal Antibody to Phosphodiesterase 10A (PDE10A)
PAG854Ge01 磷脂酰肌醇磷酸(PIP)多克隆抗体 Polyclonal Antibody to Phosphatidylinositol Phosphate (PIP)
PAG095Hu01 磷脂酰肌醇结合网格蛋白装配蛋白(PICALM)多克隆抗体 Polyclonal Antibody to Phosphatidylinositol Binding Clathrin Assembly Protein (PICALM)
PAG544Hu01 磷脂酰乙醇胺结合蛋白4(PEBP4)多克隆抗体 Polyclonal Antibody to Phosphatidylethanolamine Binding Protein 4 (PEBP4)
PAG543Hu01 白介素21抗体磷脂酰乙醇胺结合蛋白1(PEBP1)多克隆抗体 Polyclonal Antibody to Phosphatidylethanolamine Binding Protein 1 (PEBP1)
PAG543Mu01 磷脂酰乙醇胺结合蛋白1(PEBP1)多克隆抗体 Polyclonal Antibody to Phosphatidylethanolamine Binding Protein 1 (PEBP1)
PAG543Mu02 磷脂酰乙醇胺结合蛋白1(PEBP1)多克隆抗体 Polyclonal Antibody to Phosphatidylethanolamine Binding Protein 1 (PEBP1)