白细胞介素-18受体β链抗体
『别名』Interleukin-18 receptor beta; Accessory protein like; IL18R Beta; AcPL; IL 18RacP; IL 1R accessor protein like; IL 1R7; IL 1RacPL; IL18Rbeta; Interleukin 18 receptor accessory protein; IL18RAP; Interleukin-18 receptor accessory protein-like; IL-18R-beta; IL-18Rbeta; Accessory protein-like; AcPL; Interleukin-1 receptor 7; IL-1R-7; IL-1R7; CD218 antigen-like family member B; CD218b; I18RA_HUMAN.
『浓度』白细胞介素-18受体β链抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白细胞介素-18受体β链抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白细胞介素-18受体β链抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA363Ra01 血小板内皮细胞粘附分子1(PECAM1)多克隆抗体 Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
PAA363Mu02 血小板内皮细胞粘附分子1(PECAM1)多克隆抗体 Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
PAB530Hu01 血小板膜糖蛋白Ⅳ(GP4)多克隆抗体 Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4)
PAB530Mu01 血小板膜糖蛋白Ⅳ(GP4)多克隆抗体 Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4)
PAB530Ra01 血小板膜糖蛋白Ⅳ(GP4)多克隆抗体 Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4)
PAB568Hu01 血小板因子4V1(PF4V1)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 Variant 1 (PF4V1)
PAA172Bo01 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Cp01 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Hu01 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Mu01 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Po01 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Ra01 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Rb51 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Mu02 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Ra02 血小板因子4(PF4)多克隆抗体 Polyclonal Antibody to Platelet Factor 4 (PF4)
PAC921Hu01 血小板衍生生长因子B(PDGFB)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
PAC921Po01 血小板衍生生长因子B(PDGFB)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
PAC921Ra01 血小板衍生生长因子B(PDGFB)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
PAA528Ra01 血小板衍生生长因子A(PDGFA)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAA528Rb51 血小板衍生生长因子A(PDGFA)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAA528Mu01 血小板衍生生长因子A(PDGFA)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAA528Mu02 血小板衍生生长因子A(PDGFA)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAC922Hu01 血小板衍生生长因子受体样蛋白(PDGFRL)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL)
PAC922Mu01 血小板衍生生长因子受体样蛋白(PDGFRL)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL)
PAC922Mu02 血小板衍生生长因子受体样蛋白(PDGFRL)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL)
PAC061Hu01 血小板衍生生长因子受体β(PDGFRβ)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Receptor Beta (PDGFRb)
PAC061Mu01 血小板衍生生长因子受体β(PDGFRβ)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Receptor Beta (PDGFRb)
PAC060Hu01 血小板衍生生长因子受体α(PDGFRα)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Receptor Alpha (PDGFRa)
PAC060Ra01 血小板衍生生长因子受体α(PDGFRα)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor Receptor Alpha (PDGFRa)
PAC919Hu01 血小板衍生生长因子D(PDGFD)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor D (PDGFD)
PAC919Ra01 血小板衍生生长因子D(PDGFD)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor D (PDGFD)
PAC920Hu01 血小板衍生生长因子C(PDGFC)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor C (PDGFC)
PAC920Mu01 血小板衍生生长因子C(PDGFC)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor C (PDGFC)
PAC920Mu02 血小板衍生生长因子C(PDGFC)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor C (PDGFC)
PAC920Ra01 血小板衍生生长因子C(PDGFC)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor C (PDGFC)
PAA633Mu01 血小板衍生生长因子BB(PDGFBB)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor BB (PDGFBB)
PAA633Ra01 血小板衍生生长因子BB(PDGFBB)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor BB (PDGFBB)
PAA633Hu01 血小板衍生生长因子BB(PDGFBB)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor BB (PDGFBB)
PAA523Hu01 血小板衍生生长因子AA(PDGFAA)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor AA (PDGFAA)
PAA523Mu01 血小板衍生生长因子AA(PDGFAA)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor AA (PDGFAA)
PAA523Ra01 血小板衍生生长因子AA(PDGFAA)多克隆抗体 Polyclonal Antibody to Platelet Derived Growth Factor AA (PDGFAA)
PAC753Hu01 血小板活化因子受体(PAFR)多克隆抗体 Polyclonal Antibody to Platelet Activating Factor Receptor (PAFR)
PAB397Hu01 血小板激活因子乙酰水解酶Ⅰb3(PAFAH1B3)多克隆抗体 Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3)
PAB397Mu01 血小板激活因子乙酰水解酶Ⅰb3(PAFAH1B3)多克隆抗体 Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase Ib3 (PAFAH1B3)
PAC019Hu01 血小板激活因子乙酰水解酶2(PAFAH2)多克隆抗体 Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2)
PAC019Mu01 血小板激活因子乙酰水解酶2(PAFAH2)多克隆抗体 Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2)
PAC019Mu02 血小板激活因子乙酰水解酶2(PAFAH2)多克隆抗体 Polyclonal Antibody to Platelet Activating Factor Acetylhydrolase 2 (PAFAH2)
PAH365Hu01 丝束蛋白3(PLS3)多克隆抗体 Polyclonal Antibody to Plastin 3 (PLS3)
PAH365Hu02 丝束蛋白3(PLS3)多克隆抗体 Polyclonal Antibody to Plastin 3 (PLS3)
PAH365Hu03 丝束蛋白3(PLS3)多克隆抗体 Polyclonal Antibody to Plastin 3 (PLS3)
PAH365Mu01 丝束蛋白3(PLS3)多克隆抗体 Polyclonal Antibody to Plastin 3 (PLS3)
PAH366Hu01 丝束蛋白1白细胞介素-18受体β链抗体(PLS1)多克隆抗体 Polyclonal Antibody to Plastin 1 (PLS1)
PAA141Bo01 尿激酶型纤溶酶原激活因子受体(uPAR)多克隆抗体 Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)
PAA141Hu01 尿激酶型纤溶酶原激活因子受体(uPAR)多克隆抗体 Polyclonal Antibody to Plasminogen Activator, Urokinase Receptor (uPAR)