白介素-17B受体抗体
『别名』IL17B Receptor; Interleukin-17 receptor B; IL17RB; CRL 4; CRL4; Cytokine receptor CRL4; EVI 27; EVI27; IL 17 receptor B; IL 17 receptor homolog 1; IL 17B receptor; IL 17RB; IL 17Rh1; IL17BR; IL17RH1; Interleukin 17 receptor B; Interleukin 17 receptor homolog 1; Interleukin 17 receptor homolog; Interleukin 17B receptor; ecotropic viral integration site 27; interleukin-17 receptor B precursor; MGC5245; I17RB_MOUSE.
『浓度』白介素-17B受体抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
?白介素-17B受体抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素-17B受体抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAC719Hu01平足蛋白(PDPN)多克隆抗体Polyclonal Antibody to Podoplanin (PDPN)
PAC719Ra01平足蛋白(PDPN)多克隆抗体Polyclonal Antibody to Podoplanin (PDPN)
PAC719Mu01平足蛋白(PDPN)多克隆抗体Polyclonal Antibody to Podoplanin (PDPN)
PAC719Mu02平足蛋白(PDPN)多克隆抗体Polyclonal Antibody to Podoplanin (PDPN)
PAA938Hu01足突蛋白(PDCN)多克隆抗体Polyclonal Antibody to Podocin (PDCN)
PAA938Ra01足突蛋白(PDCN)多克隆抗体Polyclonal Antibody to Podocin (PDCN)
PAA938Mu01足突蛋白(PDCN)多克隆抗体Polyclonal Antibody to Podocin (PDCN)
PAA938Ra02足突蛋白(PDCN)多克隆抗体Polyclonal Antibody to Podocin (PDCN)
PAG186Mu01Podocan蛋白(PODN)多克隆抗体Polyclonal Antibody to Podocan (PODN)
PAG186Mu02Podocan蛋白(PODN)多克隆抗体Polyclonal Antibody to Podocan (PODN)
PAA768Hu01足盂蛋白(PCX)多克隆抗体Polyclonal Antibody to Podocalyxin (PCX)
PAA768Mu01足盂蛋白(PCX)多克隆抗体Polyclonal Antibody to Podocalyxin (PCX)
PAA768Mu02足盂蛋白(PCX)多克隆抗体Polyclonal Antibody to Podocalyxin (PCX)
PAA768Ra01足盂蛋白(PCX)多克隆抗体Polyclonal Antibody to Podocalyxin (PCX)
PAH358Hu01丛状蛋白B1(PLXNB1)多克隆抗体Polyclonal Antibody to Plexin B1 (PLXNB1)
PAH358Hu02丛状蛋白B1(PLXNB1)多克隆抗体Polyclonal Antibody to Plexin B1 (PLXNB1)
PAH358Hu03丛状蛋白B1(PLXNB1)多克隆抗体Polyclonal Antibody to Plexin B1 (PLXNB1)
PAH358Mu01丛状蛋白B1(PLXNB1)多克隆抗体Polyclonal Antibody to Plexin B1 (PLXNB1)
PAH354Hu01丛状蛋白A1(PLXNA1)多克隆抗体Polyclonal Antibody to Plexin A1 (PLXNA1)
PAB309Hu01多效生长因子(PTN)多克隆抗体Polyclonal Antibody to Pleiotrophin (PTN)
PAB309Bo01多效生长因子(PTN)多克隆抗体Polyclonal Antibody to Pleiotrophin (PTN)
PAB309Mu01多效生长因子(PTN)多克隆抗体Polyclonal Antibody to Pleiotrophin (PTN)
PAB309Ra01多效生长因子(PTN)多克隆抗体Polyclonal Antibody to Pleiotrophin (PTN)
PAB309Mu02多效生长因子(PTN)多克隆抗体Polyclonal Antibody to Pleiotrophin (PTN)
PAB309Hu02多效生长因子(PTN)多克隆抗体Polyclonal Antibody to Pleiotrophin (PTN)
PAB309Hu03多效生长因子(PTN)多克隆抗体Polyclonal Antibody to Pleiotrophin (PTN)
PAH380Hu01多型性腺瘤基因样蛋白1(PLAGL1)多克隆抗体Polyclonal Antibody to Pleiomorphic Adenoma Gene Like Protein 1 (PLAGL1)
PAC754Hu01网蛋白(PLEC)多克隆抗体Polyclonal Antibody to Plectin (PLEC)
PAA401Hu01普列克底物蛋白同源物样域家族A成员2(PHLDA2)多克隆抗体Polyclonal Antibody to Pleckstrin Homology Like Domain Family A, Member 2 (PHLDA2)
PAA401Mu01普列克底物蛋白同源物样域家族A成员2(PHLDA2)多克隆抗体Polyclonal Antibody to Pleckstrin Homology Like Domain Family A, Member 2 (PHLDA2)
PAH871Hu01含普列克底物同源物域家族A成员1(PLEKHA1)多克隆抗体Polyclonal Antibody to Pleckstrin Homology Domain Containing Family A, Member 1 (PLEKHA1)
PAH871Mu01含普列克底物同源物域家族A成员1(PLEKHA1)多克隆抗体Polyclonal Antibody to Pleckstrin Homology Domain Containing Family A, Member 1 (PLEKHA1)
PAA363Hu01血小板内皮细胞粘附分子1(PECAM1)多克隆抗体Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
PAA363Mu01血小板内皮细胞粘附分子1(PECAM1)多克隆抗体Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
PAA363Ra01血小板内皮细胞粘附分子1(PECAM1)多克隆抗体Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
PAA363Mu02血小板内皮细胞粘附分子1(PECAM1)多克隆抗体Polyclonal Antibody to Platelet/Endothelial Cell Adhesion Molecule (PECAM1)
PAB530Hu01血小板膜糖蛋白Ⅳ(GP4)多克隆抗体Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4)
PAB530Mu01血小板膜糖蛋白Ⅳ(GP4)多克隆抗体Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4)
PAB530Ra01血小板膜糖蛋白Ⅳ(GP4)多克隆抗体Polyclonal Antibody to Platelet Membrane Glycoprotein IV (GP4)
PAB568Hu01血小板因子4V1(PF4V1)多克隆抗体Polyclonal Antibody to Platelet Factor 4 Variant 1 (PF4V1)
PAA172Bo01血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Cp01血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Hu01血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Mu01血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Po01血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Ra01血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Rb51血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Mu02血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAA172Ra02血小板因子4(PF4)多克隆抗体Polyclonal Antibody to Platelet Factor 4 (PF4)
PAC921Hu01血小板衍生生长因子B(PDGFB)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
PAC921Po01血小板衍生生长因子B(PDGFB)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
PAC921Ra01血小板衍生生长因子B(PDGFB)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Subunit B (PDGFB)
PAA528Ra01血小板衍生生长因子A(PDGFA)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAA528Rb51血小板衍生生长因子A(PDGFA)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAA528Mu01血小板衍生生长因子A(PDGFA)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAA528Mu02白介素-17B受体抗体血小板衍生生长因子A(PDGFA)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Subunit A (PDGFA)
PAC922Hu01血小板衍生生长因子受体样蛋白(PDGFRL)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL)
PAC922Mu01血小板衍生生长因子受体样蛋白(PDGFRL)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL)
PAC922Mu02血小板衍生生长因子受体样蛋白(PDGFRL)多克隆抗体Polyclonal Antibody to Platelet Derived Growth Factor Receptor Like Protein (PDGFRL)