?白介素17受体抗体
『别名』Interleukin-17 receptor A; IL17RA; CDw217; hIL-17R; IL-17RA; MGC10262; IL-17 receptor A; IL-17RA; CD217; IL17 RECEPTOR; IL17FR; AW538159; I17RA_HUMAN.
『浓度』白介素17受体抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白介素17受体抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白介素17受体抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAA800Mu01 妊娠关联血浆蛋白A(PAPPA)多克隆抗体 Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA)
PAA800Hu02 妊娠关联血浆蛋白A(PAPPA)多克隆抗体 Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA)
PAA800Hu03 妊娠关联血浆蛋白A(PAPPA)多克隆抗体 Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA)
PAA800Mu02 妊娠关联血浆蛋白A(PAPPA)多克隆抗体 Polyclonal Antibody to Pregnancy Associated Plasma Protein A (PAPPA)
PAC722Hu01 前折叠蛋白亚基1(PFDN1)多克隆抗体 Polyclonal Antibody to Prefoldin Subunit 1 (PFDN1)
PAH323Hu01 黑色素瘤优先表达抗原(PRAME)多克隆抗体 Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)
PAH323Hu02 黑色素瘤优先表达抗原(PRAME)多克隆抗体 Polyclonal Antibody to Preferentially Expressed Antigen In Melanoma (PRAME)
PAA942Mu01 前-B-淋巴细胞基因1(VPREB1)多克隆抗体 Polyclonal Antibody to Pre-B-Lymphocyte Gene 1 (VPREB1)
PAA942Hu01 前-B-淋巴细胞基因1(VPREB1)多克隆抗体 Polyclonal Antibody to Pre-B-Lymphocyte Gene 1 (VPREB1)
PAA942Ra01 前-B-淋巴细胞基因1(VPREB1)多克隆抗体 Polyclonal Antibody to Pre-B-Lymphocyte Gene 1 (VPREB1)
PAM058Hu01 含PR域蛋白1(PRDM1)多克隆抗体 Polyclonal Antibody to PR Domain Containing Protein 1 (PRDM1)
PAM058Mu01 含PR域蛋白1(PRDM1)多克隆抗体 Polyclonal Antibody to PR Domain Containing Protein 1 (PRDM1)
PAM058Ra01 含PR域蛋白1(PRDM1)多克隆抗体 Polyclonal Antibody to PR Domain Containing Protein 1 (PRDM1)
PAV330Hu01 POTE锚蛋白域家族成员J(POTEJ)多克隆抗体 Polyclonal Antibody to POTE Ankyrin Domain Family, Member J (POTEJ)
PAM611Hu01 POTE锚蛋白域家族成员G(POTEG)多克隆抗体 Polyclonal Antibody to POTE Ankyrin Domain Family, Member G (POTEG)
PAM611Hu02 POTE锚蛋白域家族成员G(POTEG)多克隆抗体 Polyclonal Antibody to POTE Ankyrin Domain Family, Member G (POTEG)
PAQ277Ra01 内向整流型钾离子通道亚家族J成员5(KCNJ5)多克隆抗体 Polyclonal Antibody to Potassium Inwardly Rectifying Channel Subfamily J, Member 5 (KCNJ5)
PAP930Hu01 内向整流型钾离子通道亚家族J成员10(KCNJ10)多克隆抗体 Polyclonal Antibody to Potassium Inwardly Rectifying Channel Subfamily J, Member 10 (KCNJ10)
PAL050Hu01 钾中间小电导钙激活通道蛋白亚家族N成员2(KCNN2)多克隆抗体 Polyclonal Antibody to Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2)
PAB627Hu01 钾氯协同转运蛋白4(KCC4)多克隆抗体 Polyclonal Antibody to Potassium Chloride Cotransporters 4 (KCC4)
PAB628Hu01 钾氯协同转运蛋白3(KCC3)多克隆抗体 Polyclonal Antibody to Potassium Chloride Cotransporters 3 (KCC3)
PAB649Hu01 钾氯协同转运蛋白2(KCC2)多克隆抗体 Polyclonal Antibody to Potassium Chloride Cotransporters 2 (KCC2)
PAM974Bo01 顶体后致密板WW域结合蛋白(PAWP)多克隆抗体 Polyclonal Antibody to Postacrosomal Sheath WW Domain Binding Protein (PAWP)
PAS115Pl01 多酚氧化酶(PPO)多克隆抗体 Polyclonal Antibody to Polyphenol Oxidase (PPO)
PAS115Hu01 多酚氧化酶(PPO)多克隆抗体 Polyclonal Antibody to Polyphenol Oxidase (PPO)
PAB074Bo01 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Hu01 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Mu01 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Ra01 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Bo02 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Bo03 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Hu02 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Mu02 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Mu03 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Ra02 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAB074Ra03 多聚免疫球蛋白受体(PIGR)多克隆抗体 Polyclonal Antibody to Polymeric Immunoglobulin Receptor (PIGR)
PAF723Hu01 DNA指导聚合酶γ1(POLγ1)多克隆抗体 Polyclonal Antibody to Polymerase DNA Directed Gamma 1 (POLg1)
PAF721Hu01 DNA指导聚合酶β(POLβ)多克隆抗体 Polyclonal Antibody to Polymerase DNA Directed Beta (POLb)
PAF718Hu01 多梳家族环指蛋白4(PCGF4)多克隆抗体 Polyclonal Antibody to Polycomb Group Ring Finger Protein 4 (PCGF4)
PAH437Hu01 多溴蛋白1(PBRM1)多克隆抗体 Polyclonal Antibody to Polybromo 1 (PBRM1)
PAH437Hu02 多溴蛋白1(PBRM1)多克隆抗体 Polyclonal Antibody to Polybromo 1 (PBRM1)
PAH437Hu03 多溴蛋白1(PBRM1)多克隆抗体 Polyclonal Antibody to Polybromo 1 (PBRM1)
PAG817Mu01 多胺氧化酶(PAOX)多克隆抗体 Polyclonal Antibody to Polyamine Oxidase (PAOX)
PAG817Mu02 多胺氧化酶(PAOX)多克隆抗体 Polyclonal Antibody to Polyamine Oxidase (PAOX)
PAG307Hu01 多胺调节因子1结合蛋白1(PMFBP1)多克隆抗体 Polyclonal Antibody to Polyamine Modulated Factor 1 Binding Protein 1 (PMFBP1)
PAC984Hu01 多聚ADP核糖聚合酶4(PARP4)多克隆抗体 Polyclonal Antibody to Poly ADP Ribose Polymerase 4 (PARP4)
PAA279Mu01 多聚ADP核糖聚合酶(PARP)多克隆抗体 Polyclonal Antibody to Poly ADP Ribose Polymerase (PARP)
PAA279Hu01 多聚ADP核糖聚合酶(PARP)多克隆抗体 Polyclonal Antibody to Poly ADP Ribose Polymerase (PARP)
PAC176Hu01 多聚A特异性核糖核酸酶(PARN)多克隆抗体 Polyclonal Antibody to Poly A Specific Ribonuclease (PARN)
PAQ477Hu02 胞浆多聚A结合蛋白1样蛋白(PABPC1L)多克隆抗体 Polyclonal Antibody to Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L)
PAQ477Hu01 胞浆多聚A结合蛋白1样蛋白(PABPC1L)多克隆抗体 Polyclonal Antibody to Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L)
PAQ477Hu03 胞浆多聚A结合蛋白1样蛋白(PABPC1L)多克隆抗体 Polyclonal Antibody to Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L)
PAC757Hu01 脊髓灰质炎病毒受体相关分子4(PVRL4)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor Related Protein 4 (PVRL4)
PAB475Hu01 脊髓灰质炎病毒受体相关分子3(PVRL3)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor Related Protein 3 (PVRL3)
PAB475Mu01 脊髓灰质炎病毒受体相关分子3(PVRL3)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor Related Protein 3 (PVRL3)
PAB474Hu01 脊髓灰质炎病毒受体相关分子2(PVRL2)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor Related Protein 2 (PVRL2)
PAB474Mu01 白介素17受体抗体脊髓灰质炎病毒受体相关分子2(PVRL2)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor Related Protein 2 (PVRL2)
PAB470Hu01 脊髓灰质炎病毒受体相关分子1(PVRL1)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor Related Protein 1 (PVRL1)
PAB550Hu01 脊髓灰质炎病毒受体(PVR)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor (PVR)
PAB550Ra01 脊髓灰质炎病毒受体(PVR)多克隆抗体 Polyclonal Antibody to Poliovirus Receptor (PVR)