整合素αV抗体
『别名』CD 51; CD51; CD51 antigen; HGNC; ITGAV; Msk 8; Msk8; Vitronectin receptor alpha polypeptide; Vitronectin receptor alpha polypeptide antigen CD51; Vitronectin receptor subunit alpha; VNRA; ITAV_HUMAN; Integrin alpha-V heavy chain; Integrin alpha-V light chain; Integrin alpha-V Precursor.
『浓度』整合素αV抗体1mg/1ml
『规格』0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
整合素αV抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
整合素αV抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAH265Mu02 RalA结合蛋白1(RALBP1)多克隆抗体 Polyclonal Antibody to RalA Binding Protein 1 (RALBP1)
PAH265Hu02 RalA结合蛋白1(RALBP1)多克隆抗体 Polyclonal Antibody to RalA Binding Protein 1 (RALBP1)
PAS079Mu01 Raft交联蛋白(Raftlin)多克隆抗体 Polyclonal Antibody to Raft Linking Protein (Raftlin)
PAS079Hu01 Raft交联蛋白(Raftlin)多克隆抗体 Polyclonal Antibody to Raft Linking Protein (Raftlin)
PAR945Hu01 RAD54样蛋白2(RAD54L2)多克隆抗体 Polyclonal Antibody to RAD54 Like Protein 2 (RAD54L2)
PAP750Hu01 RAD51同源物(RAD51)多克隆抗体 Polyclonal Antibody to RAD51 Homolog (RAD51)
PAP751Hu01 RAD23同源物B(RAD23B)多克隆抗体 Polyclonal Antibody to RAD23 Homolog B (RAD23B)
PAE293Hu01 Rac-GTP酶激活蛋白1(RACGAP1)多克隆抗体 Polyclonal Antibody to Rac-GTPase Activating Protein 1 (RACGAP1)
PAK305Hu01 RAS癌基因家族成员RAB5A(RAB5A)多克隆抗体 Polyclonal Antibody to RAB5A, Member RAS Oncogene Family (RAB5A)
PAK305Mu01 RAS癌基因家族成员RAB5A(RAB5A)多克隆抗体 Polyclonal Antibody to RAB5A, Member RAS Oncogene Family (RAB5A)
PAK305Mu02 RAS癌基因家族成员RAB5A(RAB5A)多克隆抗体 Polyclonal Antibody to RAB5A, Member RAS Oncogene Family (RAB5A)
PAM679Hu01 RAS癌基因家族成员RAB37(RAB37)多克隆抗体 Polyclonal Antibody to RAB37, Member RAS Oncogene Family (RAB37)
PAJ783Hu01 RAS癌基因家族成员RAB1A(RAB1A)多克隆抗体 Polyclonal Antibody to RAB1A, Member RAS Oncogene Family (RAB1A)
PAK552Ge01 喹啉酸(QA)多克隆抗体 Polyclonal Antibody to Quinolinic Acid (QA)
PAC758Hu01 醌型二氢蝶啶还原酶(QDPR)多克隆抗体 Polyclonal Antibody to Quinoid Dihydropteridine Reductase (QDPR)
PAC759Hu01 静止素Q6硫基氧化酶1(QSOX1)多克隆抗体 Polyclonal Antibody to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)
PAC759Hu02 静止素Q6硫基氧化酶1(QSOX1)多克隆抗体 Polyclonal Antibody to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)
PAC759Mu01 静止素Q6硫基氧化酶1(QSOX1)多克隆抗体 Polyclonal Antibody to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)
PAC759Mu02 静止素Q6硫基氧化酶1(QSOX1)多克隆抗体 Polyclonal Antibody to Quiescin Q6 Sulfhydryl Oxidase 1 (QSOX1)
PAA588Hu01 M2-型丙酮酸激酶(PKM2)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)
PAA588Mu01 M2-型丙酮酸激酶(PKM2)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)
PAA588Ra01 M2-型丙酮酸激酶(PKM2)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)
PAA588Hu02 M2-型丙酮酸激酶(PKM2)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)
PAA588Mu02 M2-型丙酮酸激酶(PKM2)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)
PAA208Ca01 丙酮酸激酶(PK)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Liver And RBC (PK)
PAA208Hu01 丙酮酸激酶(PK)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Liver And RBC (PK)
PAA208Mu01 丙酮酸激酶(PK)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Liver And RBC (PK)
PAA208Ra01 丙酮酸激酶(PK)多克隆抗体 Polyclonal Antibody to Pyruvate Kinase, Liver And RBC (PK)
PAA887Hu01 丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Phosphatase (PDP)
PAA887Mu01 丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Phosphatase (PDP)
PAA958Hu01 丙酮酸脱氢酶激酶同工酶4(PDK4)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)
PAA958Mu01 丙酮酸脱氢酶激酶同工酶4(PDK4)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)
PAA958Mu02 丙酮酸脱氢酶激酶同工酶4(PDK4)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4)
PAD463Mu01 丙酮酸脱氢酶激酶同工酶3(PDK3)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 3 (PDK3)
PAD464Mu01 丙酮酸脱氢酶激酶同工酶2(PDK2)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 2 (PDK2)
PAC718Mu01 丙酮酸脱氢酶激酶同工酶1(PDK1)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)
PAC718Hu01 丙酮酸脱氢酶激酶同工酶1(PDK1)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)
PAC718Ra01 丙酮酸脱氢酶激酶同工酶1(PDK1)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Kinase Isozyme 1 (PDK1)
PAH426Hu01 丙酮酸脱氢酶复合体成分X(PDHX)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Complex Component X (PDHX)
PAH426Mu01 丙酮酸脱氢酶复合体成分X(PDHX)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Complex Component X (PDHX)
PAB923Hu01 丙酮酸脱氢酶β(PDHβ)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
PAB923Mu01 丙酮酸脱氢酶β(PDHβ)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
PAB923Mu02 丙酮酸脱氢酶β(PDHβ)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Beta (PDHb)
PAA776Hu01 丙酮酸脱氢酶α(PDHα)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Alpha (PDHa)
PAA776Mu01 丙酮酸脱氢酶α(PDHα)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Alpha (PDHa)
PAA776Ra01 丙酮酸脱氢酶α(PDHα)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Alpha (PDHa)
PAA776Mu02 丙酮酸脱氢酶α(PDHα)多克隆抗体 Polyclonal Antibody to Pyruvate Dehydrogenase Alpha (PDHa)
PAH430Mu01 丙酮酸羧化酶(PC)多克隆抗体 Polyclonal Antibody to Pyruvate Carboxylase (PC)
PAH430Hu01 丙酮酸羧化酶(PC)多克隆抗体 Polyclonal Antibody to Pyruvate Carboxylase (PC)
PAH430Mu02 丙酮酸羧化酶(PC)多克隆抗体 Polyclonal Antibody to Pyruvate Carboxylase (PC)
PAB724Hu01 吡哆醛激酶(PDXK)多克隆抗体 Polyclonal Antibody to Pyridoxal Kinase (PDXK)
PAB724Ra01 吡哆醛激酶(PDXK)多克隆抗体 Polyclonal Antibody to Pyridoxal Kinase (PDXK)
PAB724Mu01 吡哆醛激酶(PDXK)多克隆抗体 Polyclonal Antibody to Pyridoxal Kinase (PDXK)
PAM076Hu01 Pygopus同源物2(PYGO2)多克隆抗体 Polyclonal Antibody to Pygopus Homolog 2 (PYGO2)
PAM077Hu01 Pygopus同源物1(PYGO1)多克隆抗体 Polyclonal Antibody to Pygopus Homolog 1 (PYGO1)
PAE206Hu01 嘌呤霉素敏感性氨肽酶(PSA)多克隆抗体 Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)
PAE206Mu01 嘌呤霉素敏感性氨肽酶(PSA)多克隆抗体 Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)
PAE206Hu02 嘌呤霉素敏感性氨肽酶(PSA)多克隆抗体 Polyclonal Antibody to Puromycin Sensitive Aminopeptidase (PSA)
PAG084Hu01 整合素αV抗体嘌呤能受体P2Y14(P2RY14)多克隆抗体 Polyclonal Antibody to Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14)
PAG086Hu01 嘌呤能受体P2Y12(P2RY12)多克隆抗体 Polyclonal Antibody to Purinergic Receptor P2Y, G Protein Coupled 12 (P2RY12)
PAG079Hu01 嘌呤能受体P2Y1(P2RY1)多克隆抗体 Polyclonal Antibody to Purinergic Receptor P2Y, G Protein Coupled 1 (P2RY1)