?白细胞介素-10受体a抗体
『别名』Interleukin-10 Receptor; CD210; CDW210A; CDw210a; CDw210a antigen; HIL 10R; HIL10R; IL 10R 1; IL 10R A; IL 10R; IL 10R1; IL10R; IL10R1; Interleukin 10 receptor alpha; Interleukin 10 receptor alpha chain; I10R1_HUMAN; Interleukin-10 receptor subunit alpha; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; Interleukin-10 receptor bunit 1; IL-10R subunit 1; IL-10R1.
『浓度』白细胞介素-10受体a抗体1mg/1ml
『规格』0.2ml/100μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
白细胞介素-10受体a抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
白细胞介素-10受体a抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
PAE382Ra01 钠/葡萄糖协同转运蛋白2(SGLT2)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 2 (SGLT2)
PAE381Hu01 钠/葡萄糖协同转运蛋白1(SGLT1)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 1 (SGLT1)
PAE381Mu01 钠/葡萄糖协同转运蛋白1(SGLT1)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 1 (SGLT1)
PAE381Mu02 钠/葡萄糖协同转运蛋白1(SGLT1)多克隆抗体 Polyclonal Antibody to Sodium/Glucose Cotransporter 1 (SGLT1)
PAE385Hu01 钠碘运输蛋白(NIS)多克隆抗体 Polyclonal Antibody to Sodium Iodide Symporter (NIS)
PAE505Hu01 钠耦合中性氨基酸转运蛋白1(SNAT1)多克隆抗体 Polyclonal Antibody to Sodium Coupled Neutral Amino Acid Transporter 1 (SNAT1)
PAH163Hu01 NAP关联蛋白(SNAPAP)多克隆抗体 Polyclonal Antibody to SNAP Associated Protein (SNAPAP)
PAK089Hu01 Snail同源物1(SNAI1)多克隆抗体 Polyclonal Antibody to Snail Homolog 1 (SNAI1)
PAC856Hu01 平滑肌细胞分化特异性抗原(SMTN)多克隆抗体 Polyclonal Antibody to Smoothelin (SMTN)
PAC856Ra01 平滑肌细胞分化特异性抗原(SMTN)多克隆抗体 Polyclonal Antibody to Smoothelin (SMTN)
PAG152Hu01 小泛素相关修饰蛋白2(SUMO2)多克隆抗体 Polyclonal Antibody to Small Ubiquitin Related Modifier Protein 2 (SUMO2)
PAG154Hu01 小泛素相关修饰蛋白1(SUMO1)多克隆抗体 Polyclonal Antibody to Small Ubiquitin Related Modifier Protein 1 (SUMO1)
PAG154Mu01 小泛素相关修饰蛋白1(SUMO1)多克隆抗体 Polyclonal Antibody to Small Ubiquitin Related Modifier Protein 1 (SUMO1)
PAB128Hu01 小核糖核蛋白多肽D1(SNRPD1)多克隆抗体 Polyclonal Antibody to Small Nuclear Ribonucleoprotein Polypeptide D1 (SNRPD1)
PAB374Hu01 小核糖核蛋白多肽C(SNRPC)多克隆抗体 Polyclonal Antibody to Small Nuclear Ribonucleoprotein Polypeptide C (SNRPC)
PAC266Hu01 小异二聚体伴侣(SHP)多克隆抗体 Polyclonal Antibody to Small Heterodimer Partner (SHP)
PAB840Hu01 小乳腺表皮粘蛋白(SBEM)多克隆抗体 Polyclonal Antibody to Small Breast Epithelial Mucin (SBEM)
PAD353Hu01 Slit同源物3(Slit3)多克隆抗体 Polyclonal Antibody to Slit Homolog 3 (Slit3)
PAD353Mu01 Slit同源物3(Slit3)多克隆抗体 Polyclonal Antibody to Slit Homolog 3 (Slit3)
PAD353Ra01 Slit同源物3(Slit3)多克隆抗体 Polyclonal Antibody to Slit Homolog 3 (Slit3)
PAD353Mu02 Slit同源物3(Slit3)多克隆抗体 Polyclonal Antibody to Slit Homolog 3 (Slit3)
PAD353Ra02 Slit同源物3(Slit3)多克隆抗体 Polyclonal Antibody to Slit Homolog 3 (Slit3)
PAD353Mu03 Slit同源物3(Slit3)多克隆抗体 Polyclonal Antibody to Slit Homolog 3 (Slit3)
PAA672Hu01 Slit同源物2(Slit2)多克隆抗体 Polyclonal Antibody to Slit Homolog 2 (Slit2)
PAA672Mu01 Slit同源物2(Slit2)多克隆抗体 Polyclonal Antibody to Slit Homolog 2 (Slit2)
PAA672Ra01 Slit同源物2(Slit2)多克隆抗体 Polyclonal Antibody to Slit Homolog 2 (Slit2)
PAA672Hu02 Slit同源物2(Slit2)多克隆抗体 Polyclonal Antibody to Slit Homolog 2 (Slit2)
PAA672Mu02 Slit同源物2(Slit2)多克隆抗体 Polyclonal Antibody to Slit Homolog 2 (Slit2)
PAD354Hu01 Slit同源物1(Slit1)多克隆抗体 Polyclonal Antibody to Slit Homolog 1 (Slit1)
PAD354Mu01 Slit同源物1(Slit1)多克隆抗体 Polyclonal Antibody to Slit Homolog 1 (Slit1)
PAD354Ra01 Slit同源物1(Slit1)多克隆抗体 Polyclonal Antibody to Slit Homolog 1 (Slit1)
PAD354Ra03 Slit同源物1(Slit1)多克隆抗体 Polyclonal Antibody to Slit Homolog 1 (Slit1)
PAD354Ra02 Slit同源物1(Slit1)多克隆抗体 Polyclonal Antibody to Slit Homolog 1 (Slit1)
PAD354Mu02 Slit同源物1(Slit1)多克隆抗体 Polyclonal Antibody to Slit Homolog 1 (Slit1)
PAD354Hu02 Slit同源物1(Slit1)多克隆抗体 Polyclonal Antibody to Slit Homolog 1 (Slit1)
PAJ454Hu01 丫叉同源物3(SSH3)多克隆抗体 Polyclonal Antibody to Slingshot Homolog 3 (SSH3)
PAJ455Hu01 丫叉同源物2(SSH2)多克隆抗体 Polyclonal Antibody to Slingshot Homolog 2 (SSH2)
PAJ456Hu01 丫叉同源物1(SSH1)多克隆抗体 Polyclonal Antibody to Slingshot Homolog 1 (SSH1)
PAM234Hu01 SKI样癌基因(SKIL)多克隆抗体 Polyclonal Antibody to SKI Like Oncogene (SKIL)
PAD199Mu01 干燥综合征抗原B(SSB)多克隆抗体 Polyclonal Antibody to Sjogren Syndrome Antigen B (SSB)
PAD199Mu02 干燥综合征抗原B(SSB)多克隆抗体 Polyclonal Antibody to Sjogren Syndrome Antigen B (SSB)
PAH122Hu01 前列腺六跨膜表皮抗原2(STEAP2)多克隆抗体 Polyclonal Antibody to Six Transmembrane Epithelial Antigen Of The Prostate 2 (STEAP2)
PAE917Hu01 沉默调节蛋白7(SIRT7)多克隆抗体 Polyclonal Antibody to Sirtuin 7 (SIRT7)
PAE917Ra01 沉默调节蛋白7(SIRT7)多克隆抗体 Polyclonal Antibody to Sirtuin 7 (SIRT7)
PAE917Ra02 沉默调节蛋白7(SIRT7)多克隆抗体 Polyclonal Antibody to Sirtuin 7 (SIRT7)
PAE916Hu01 沉默调节蛋白6(SIRT6)多克隆抗体 Polyclonal Antibody to Sirtuin 6 (SIRT6)
PAE916Mu01 沉默调节蛋白6(SIRT6)多克隆抗体 Polyclonal Antibody to Sirtuin 6 (SIRT6)
PAE916Ra01 沉默调节蛋白6(SIRT6)多克隆抗体 Polyclonal Antibody to Sirtuin 6 (SIRT6)
PAE916Mu02 沉默调节蛋白6(SIRT6)多克隆抗体 Polyclonal Antibody to Sirtuin 6 (SIRT6)
PAE915Hu01 沉默调节蛋白5(SIRT5)多克隆抗体 Polyclonal Antibody to Sirtuin 5 (SIRT5)
PAE915Mu01 沉默调节蛋白5(SIRT5)多克隆抗体 Polyclonal Antibody to Sirtuin 5 (SIRT5)
PAE915Ra01 沉默调节蛋白5(SIRT5)多克隆抗体 Polyclonal Antibody to Sirtuin 5 (SIRT5)
PAE914Hu01 沉默调节蛋白4(SIRT4)多克隆抗体 Polyclonal Antibody to Sirtuin 4 (SIRT4)
PAE914Mu01 沉默调节蛋白4(SIRT4)多克隆抗体 Polyclonal Antibody to Sirtuin 4 (SIRT4)
PAE914Ra01 沉默调节蛋白4(SIRT4)多克隆抗体 Polyclonal Antibody to Sirtuin 4 (SIRT4)
PAE913Hu01 沉默调节蛋白3(SIRT3)多克隆抗体 Polyclonal Antibody to Sirtuin 3 (SIRT3)
PAE913Ra01 沉默调节蛋白3(SIRT3)多克隆抗体 Polyclonal Antibody to Sirtuin 3 (SIRT3)
PAE913Mu01 沉默调节蛋白3(SIRT3)多克隆抗体 Polyclonal Antibody to Sirtuin 3 (SIRT3)
PAE913Mu02 沉默调节蛋白3(SIRT3)多克隆抗体 Polyclonal Antibody to Sirtuin 3 (SIRT3)
PAA430Hu01 沉默调节蛋白2白细胞介素-10受体a抗体(SIRT2)多克隆抗体 Polyclonal Antibody to Sirtuin 2 (SIRT2)
PAA430Ra01 沉默调节蛋白2(SIRT2)多克隆抗体 Polyclonal Antibody to Sirtuin 2 (SIRT2)
PAA430Mu01 沉默调节蛋白2(SIRT2)多克隆抗体 Polyclonal Antibody to Sirtuin 2 (SIRT2)
PAE912Hu01 沉默调节蛋白1(SIRT1)多克隆抗体 Polyclonal Antibody to Sirtuin 1 (SIRT1)