甲型流感病毒血凝素抗体
『别名』HA; HA2; Hemagglutinin; Influenza A Virus [A/California/04/2009(H1N1)].
『浓度』甲型流感病毒血凝素抗体1mg/1ml
『规格』0.1ml/100μg 0.2ml/200μg
『抗体来源』Rabbit
抗体分子是生物学和医学领域用途最为广泛的蛋白分子。抗体作为疾病预防、诊断和治疗的制剂已有上百年的发展历史。随着生命科学研究的迅猛发展,抗体工程在生物技术领域越来越占有非常重要的地位。我公司可为您提供快速的、高质量的和经济的多克隆抗体制备服务,并将成为您在科研及生产中的得力助手。
我公司免费提供抗血清亲合层析纯化服务,得到目的蛋白的抗体IgG。
我公司还免费提供真空冷冻抽干服务,得到冻干粉状的抗体IgG产品,以便产品的使用及长期保存。
甲型流感病毒血凝素抗体洗脱问题
抗体组目前采用的是低pH洗脱方法,按道理说,这个方法应该可以洗脱掉纯大部分抗体,但是有时候出现了一些顽固抗体很难洗脱,大家可以考虑其它的洗脱方法,beads说明书上提到了五大类办法,我觉得比较可靠的方法只有两类,一是改变pH,二是改变离子浓度,另外几种办法要么会带入有机试剂,要么会带入氨基,所以不太适用。改变pH的办法除了用低pH,还可以考虑用高pH来洗脱,好像没多少人尝试过这种办法,在确实没有办法的时候推荐尝试一下。
甲型流感病毒血凝素抗体其它问题
1、看了很多抗体纯化的资料,不管是从血清中纯化多抗还是从腹水中纯化单抗,都推荐大家在上柱子之前把血清或腹水稀释2-5倍,另外把血清或腹水采用0.22微米或0.45微米孔径的膜过滤一次。过滤这一步我们是没条件做的了,但是稀释这一步推荐大家试一试,有文献说稀释可以提高回收率
2、封闭这一步做完后,是不是考虑可以用HCl洗一次?连在beads上的蛋白有的是蛋白间非特异性结合上去的,洗脱之前的任何步骤也许都无法洗干净它们,所以这里把它们都洗掉,以免最后都洗到抗体里去了。
3、CNBr活化的琼脂糖或葡聚糖本身的缺陷:这个非特异性结合影响最大的是和血清中的物质结合了。而血清中大部分蛋白都是免疫球蛋白,其中包括我们要的抗体,它们都带有一定的负电,所以都会和这个部位进行非特异性结合,在洗脱前,有一步是用pH5.0的Gly去洗掉与抗原非特异性结合的抗体的,但是它也会洗掉直接与beads非特异结合的蛋白,也包括我们要的抗体,至于这一步会丢掉多少抗体,好像没有人测过。
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PAC874Hu01 斯钙素1(STC1)多克隆抗体 Polyclonal Antibody to Stanniocalcin 1 (STC1)
PAC874Mu01 斯钙素1(STC1)多克隆抗体 Polyclonal Antibody to Stanniocalcin 1 (STC1)
PAC874Ra01 斯钙素1(STC1)多克隆抗体 Polyclonal Antibody to Stanniocalcin 1 (STC1)
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PAQ342Hu01 SRSF蛋白激酶3(SRPK3)多克隆抗体 Polyclonal Antibody to SRSF Protein Kinase 3 (SRPK3)
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PAB372Hu01 鳞状细胞癌抗原1(SCCA1)多克隆抗体 Polyclonal Antibody to Squamous Cell Carcinoma Antigen 1 (SCCA1)
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PAL008Ra01 侧支发芽因子同源物2(SPRY2)多克隆抗体 Polyclonal Antibody to Sprouty Homolog 2 (SPRY2)
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PAF396Mu02 脊椎蛋白2(SPON2)多克隆抗体 Polyclonal Antibody to Spondin 2 (SPON2)
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