大鼠I型胶原羧基端交联肽(CTx-I)ELISA试剂盒
2015-09-10 15:57
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资料摘要:
Rat CTx-I
FOR RESEARCH USE ONLY
Assay range:0.8nmol/L - 35nmol/L 96 determinations
Purpose
This kit allows for the determination of CTx-I concentrations in Rat serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Rat CTx-I level in the sample, use Purified Rat CTx-I antibody to coat microtiter plate wells, make solid-phase antibody, then add CTx-I to wells, Combined CTx-I antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat CTx-I in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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大鼠I型胶原羧基端交联肽(CTx-I)英文说明书.doc
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