大鼠P16ELISA试剂盒
2015-09-10 15:58
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资料摘要:
Rat P16
FOR RESEARCH USE ONLY
Assay range:3.0ng/L -100 ng/L 96 determinations
Purpose
This kit allows for the determination of P16 concentrations in Rat serum, cell culture supernates and other biological fluids
Principle of the assay
The kit assay Rat P16 level in the sample, use Purified Rat P16 antibody to coat microtiter plate wells, make solid-phase antibody, then add P16 to wells, Combined P16 antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat P16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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