大豆凝集素(SBA)ELISA试剂盒
2015-09-10 15:48
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资料摘要:
Plant SBA
FOR RESEARCH USE ONLY
Assay range:50ng/L -850 ng/L 96 determinations
Purpose
For the quantitative in vitro determination of SBA concentrations in Plant tissue ,cell and other samples.
Principle of the assay
The kit assay SBA level in the sample,use Purified SBA antibody to coat microtiter plate wells, make solid-phase antibody, then add SBA to wells, Combined SBA antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SBA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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