Porcine(LDH)ELISA Kit

Porcine(LDH)ELISA Kit Porcine Lactate Dehydrogenase (LDH) ELISA Kit FOR RESEARCH USE ONLY Purpose This kit allows for the determination of LDH concentrations in Porcineserum, blood plasma, and other biological fluids. Principle of the assay The kit assay Porcine LDH level in the sample，use Purified Porcine LDHantibody to coat microtiter plate wells, make solid-phase antibody, then addLDH to wells, Combined LDH antibody which With HRP labeled, becomeantibody - antigen - enzyme-antibody complex, after washing Completely, AddTMB substrate solution,TMB substrate becomes blue color At HRPenzyme-catalyzed, reaction is terminated by the addition of a sulphuric acidsolution and the color change is measured spectrophotometrically at awavelength of 450 nm. The concentration of LDH in the samples is thendetermined by comparing the O.D. of the samples to the standard curve.