猪乳酸脱氢酶(LDH)ELISA试剂盒英文说明

猪乳酸脱氢酶(LDH)ELISA试剂盒英文说明 Specimen requirements 1. serum- coagulation at room temperature 10-20 mins，centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitationappeared, Centrifugal again. 2. plasma-use suited EDTA or citrate plasma as an anticoagulant,mix 10-20mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. removesupernatant, If precipitation appeared, Centrifugal again. 3. Urine-collect sue a sterile container, centrifugation 20-min at the speed of2000-3000 r.p.m. remove supernatant, If precipitation appeared,Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluidReference to it. 4. cell culture supernatant-detect secretory components, collect sue asterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m.remove supernatant,detect the composition of cells, Dilut cell suspensionwith PBS（PH7.2-7.4）, Cell concentration reached 1 million / ml, repeatedfreeze-thaw cycles, damage cells and release of intracellular components,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,If precipitation appeared, Centrifugal again. 5. Tissue samples- After cutting samples, check the weight,add PBS（PH7.2-7.4）, Rapidly frozen with liquid nitrogen, maintain samples at 2-8℃ after melting,add PBS（PH7.4）, Homogenized by hand or Grinders,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant. 6. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles. 7. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active.