神经节肽抗体英文名称 Anti-Galanin
中文名称 神经节肽抗体
别 名 GAL 1; GAL; GAL1; Galanin message associated peptide; Galanin precursor; Galanin related peptide; GALN; GLNN; GMAP; MGC40167; GALA_HUMAN.
浓 度 1mg/1ml
规 格 0.2ml/200μg
纯化的抗体可通过不同的途径获取,有些神经节肽抗体可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对神经节肽抗体能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。
抗体来源 Mouse
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep, Guinea Pig
产品类型 一抗
研究领域 免疫学 神经生物学
蛋白分子量 predicted molecular weight: 3/13kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Galanin (33-62aa)
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神经节肽抗体产品介绍 Galanin is a 30 amino acid neuropeptide widely distributed in the peripheral and central nervous systems and endocrine system. The amino acid sequence of galanin is very conserved (almost 90% among species), indicating the importance of the molecule. In the brain, the highest concentrations are observed in the hypothalamus and particularly in the nerve terminals of the median eminence.
Function : Contracts smooth muscle of the gastrointestinal and genitourinary tract, regulates growth hormone release, modulates insulin release, and may be involved in the control of adrenal secretion.
Subcellular Location : Secreted.
Similarity : Belongs to the galanin family.
神经节肽(Galanin)是由30个氨基酸残基组成的神经肽。最初认为只在相当小的背根神经节细胞群体及脊髓神经元中出现,后发现在消化道及胰腺中也广泛分布。与感觉的调整有关,对痛觉传递及脊髓兴奋起抑制作用,在很多禽类动物中有表达,是中枢神经系统及内分泌胰腺的神经调节肽。