神经元突触前膜蛋白抗体

供货周期： 7天

品牌： Abcam

型号： 0.1ml/100μg

货号：

报价： ¥1

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产品介绍

神经元突触前膜蛋白抗体勿使用无霜冷冻箱，因为会冷冻循环，这样会损伤抗体。

英文名称 Anti-Amphiphysin

中文名称神经元突触前膜蛋白抗体

别名 Amphiphysin; Amp; AMPH 1; AMPH1; Amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen); Amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen); Amphiphysin I; CG8604; Damp; dAmph; Dmel\CG8604; HGNC:471; MGC49429; Stiff man syndrome with breast cancer 128kD autoantigen; Stiff man syndrome with breast cancer 128kDa autoantigen; AMPH_HUMAN.

浓度 1mg/1ml

规格 0.2ml/200μg

抗体来源 Rabbit

克隆类型 polyclonal

交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Rabbit

神经元突触前膜蛋白抗体产品类型一抗

研究领域细胞生物免疫学神经生物学

蛋白分子量 predicted molecular weight: 76kDa

性状 Lyophilized or Liquid

免疫原 KLH conjugated synthetic peptide derived from human Amphiphysin

亚型 IgG

纯化方法 affinity purified by Protein A

储存液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

神经元突触前膜蛋白抗体产品介绍 Amphiphysin is associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants (Amphiphysin 1 and Amphiphysin 2) encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined.

Function : May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

Subunit : Heterodimer with BIN1. Binds SH3GLB1 and AP2A2. Interacts with AP2B1.

Subcellular Location : Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton.

Tissue Specificity : Neurons, certain endocrine cell types and spermatocytes.

Similarity : Contains 1 BAR domain.

Contains 1 SH3 domain.

Database links : UniProtKB/Swiss-Prot: P49418.1

我们应该如何储存抗体且最大限度地提高保质期，简单地说：

抗体应在（-20℃）中冷冻干燥或浓缩的液体形式，直到需要，避免反复冷冻/解冻周期，降低抗体含量和浓度。相反，冻结在单次使用的等分试样重组抗体或添加防冻剂（甘油或乙二醇），从而使料液可以保持在-20℃下为液体形式，以使移液没有“解冻”。

在4℃下存放，避免抗体（或其他蛋白质溶液）超过几天浓缩存量（> 0.5毫克）通常是稳定的，神经元突触前膜蛋白抗体微生物添加剂，如叠氮化钠。不要试图保存稀释（<0.1mg/ml时）抗体溶液稳定，除非通过添加“载体”蛋白质或特殊添加剂。