内收蛋白抗体

内收蛋白抗体英文名称  Anti-beta Adducin 

中文名称  内收蛋白抗体 

别    名  ADD 2; ADD B; ADD2; ADDB; ADDL; Adducin 2 (beta); Adducin 2; Adducin like; Adducin like protein 70; Adducin2; Beta adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; Erythrocyte adducin subunit beta; MGC3339; MGC44427; ADDB_HUMAN. 

浓    度  1mg/1ml 

规 格  0.1ml/100μg  0.2ml/200μg    

抗体来源  Rabbit  

克隆类型  polyclonal 

交叉反应  Human, Mouse, Rat, Dog, Horse, Rabbit   

产品类型  一抗    

研究领域  细胞生物 信号转导 结合蛋白 细胞类型标志物 细胞骨架  

蛋白分子量  predicted molecular weight: 80kDa 

性    状  Lyophilized or Liquid 

免 疫 原  KLH conjugated synthetic peptide derived from human beta Adducin protein 

亚    型  IgG 

纯化方法  affinity purified by Protein A 

储 存 液  0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide 

产品应用   WB=1:200-1000  ELISA=1:500-5000  IP=1:20-100  IHC-P=1:200-1000  IHC-F=1:200-1000  IF=1:200-1000 

（石蜡切片需做抗原修复） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

内收蛋白抗体产品介绍 Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha and beta adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha and gamma adducins are ubiquitously expressed. In contrast, beta adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.

Function : Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.

Subunit : Heterodimer of an alpha and a beta subunit.

Subcellular Location : Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Tissue Specificity : Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

Post-translational modifications : The N-terminus is blocked.

Similarity : Belongs to the aldolase class II family. Adducinbn subfamily.

Database links : UniProtKB/Swiss-Prot: Q05764.4 rat

UniProtKB/Swiss-Prot: P35612.3 human

内收蛋白(Adducin）是一种膜骨架蛋白，内收蛋白可与肌动蛋白及血影蛋白复合体结合，并且通过Ca2+和钙调蛋白的作用影响骨架蛋白的稳定性，从而影响红细胞的形态.