胶质纤维酸性蛋白抗体英文名称 Anti-GFAP
中文名称 胶质纤维酸性蛋白抗体
别 名 Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg 1ml/1mg
纯化的抗体可通过不同的途径获取,有些胶质纤维酸性蛋白抗体可通过下述方法制备或从商家购买。从商家购买的抗体,通常附有正确的储存方法。
1)工作液应在4℃下融化并存放,可能稳定达数月。
2)如果没有特殊原因而避免使用叠氮钠,亦可加入叠氮钠,浓度为0.02%。将纯化的抗体样本分装成合适的体积,于-20℃保存。
3)纯化的抗体溶液应以较高的浓度(如lmg/m1)在中性pH下保存。:常用的抗体储存浓度高达l0mg/ml。较低浓度的抗体冻存前应浓缩。所有标准的浓缩方法(如超滤法),皆可使用。还有一个简单的方法是用蛋白A或蛋白G亲和柱来浓缩溶液。如果纯化的抗体不是用于标记,可将它们以较低浓度储存于加有1%BSA的溶液中。
4)经纯化制备的抗体在常用的缓冲液中是稳定的。其DH应保持在中性左右。如果pH在7-8之间,即使保存多年,对抗体也无损害。多数情况下,盐浓度适于保持在0-150mmol/L之间,但在长期存放的抗体中,盐溶液浓度高达500mmol/L时,对胶质纤维酸性蛋白抗体能有损害。如果没有其他说明.律议用PBS或50mmol/LTris(DH8.0)溶液长期存放抗体。
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep
产品类型 一抗
研究领域 肿瘤 细胞生物 免疫学 神经生物学
蛋白分子量 predicted molecular weight: 48kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human GFAP
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
胶质纤维酸性蛋白抗体产品介绍 Astrocyte Marker
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
Function : GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subunit : Interacts with SYNM (By similarity). Isoform 3 interacts with PSEN1 (via N-terminus).
Subcellular Location : Cytoplasm. Note=Associated with intermediate filaments.
Tissue Specificity : Expressed in cells lacking fibronectin.
Post-translational modifications : Phosphorylated by PKN1.
DISEASE : Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
Similarity : Belongs to the intermediate filament family.
Database links :
Entrez Gene: 281189 Cow
Entrez Gene: 2670 Human
Entrez Gene: 14580 Mouse
Entrez Gene: 24387 Rat
Omim: 137780 Human
SwissProt: Q28115 Cow
UniProtKB/Swiss-Prot: P14136.1
SwissProt: P14136 Human
SwissProt: P03995 Mouse
星形胶质细胞标志物 (Astrocyte Marker)
GFAP是一个56kDa的中间丝蛋白(intermediate filament,IF),在中枢神经系统发育期是一个特异性的标志物,以区别星形细胞和其它胶质细胞。GFAP表达在皮层和海马,急、慢性皮质酮治疗时表达减少。
GFAP可以和人、大鼠、小鼠的GFAP反应,在正常和肿瘤性的星形胶质细胞阳性表达,而神经节细胞、神经元、成纤维细胞、少突胶质细胞和这些细胞来源的肿瘤细胞阴性表达,主要用于星形胶质瘤等中枢神经系统肿瘤的诊断和鉴别诊断,GFAP的缺乏可导致AD病。