产品名称:犬胰岛素(INS)ELISA Kit 英文名称:Dog Insulin,INS ELISA Kit 产品类型： ELISA Kit 货号： CSB-E11327c 价格： 3800 规格： 96T 种属： Dog 待测物名称： insulin 缩写： INS 样本类型： serum, plasma, tissue homogenates 检测范围： 0.625 灵敏度： 0.156 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 产品名称                                         货号                 规格 Human Free Thyroxine,FT4 ELISA Kit            CSB-E05078h             96T Chicken Insulin ELISA kit                     CSB-E13293C             96T Dog Insulin,INS ELISA Kit                     CSB-E11327c             96T rabbit Insulin,INS ELISA Kit                  CSB-E06992Rb            96T Rat Free Thyroxine,FT4 ELISA Kit              CSB-E05079r             96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05077m     96T Pig Insulin,INS ELISA Kit                     CSB-E06828p             96T Guinea pig insulin (INS) ELISA kit            CSB-EL011742GU          96T Fish Insulin,INS ELISA Kit                    CSB-E12123Fh            96T Human Insulin,INS ELISA Kit                   CSB-E05069h             96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit   CSB-E05076r      96T Bovine Insulin ELISA kit                      CSB-E11993B             96T Rat Insulin,INS ELISA Kit                     CSB-E05070r             96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit      CSB-E05074m             96T Sheep Insulin(INS) ELISA Kit                  CSB-E17044Sh            96T Monkey Insulin,INS ELISA Kit                  CSB-E10047Mo            96T Horse insulin (INS) ELISA kit                 CSB-EL011742HO          96T Goat Insulin(INS)ELISA Kit                    CSB-E17653G             96T Cat insulin (INS) ELISA kit                   CSB-EL011742CA          96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05075h     96T

产品名称:猴胰岛素(INS)ELISA Kit 英文名称:Monkey Insulin,INS ELISA Kit 产品类型： ELISA Kit 货号： CSB-E10047Mo 价格： 3800 规格： 96T 种属： Monkey 待测物名称： Insulin,INS 缩写： INS 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 产品名称                                         货号                 规格 Human Free Thyroxine,FT4 ELISA Kit            CSB-E05078h             96T Chicken Insulin ELISA kit                     CSB-E13293C             96T Dog Insulin,INS ELISA Kit                     CSB-E11327c             96T rabbit Insulin,INS ELISA Kit                  CSB-E06992Rb            96T Rat Free Thyroxine,FT4 ELISA Kit              CSB-E05079r             96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05077m     96T Pig Insulin,INS ELISA Kit                     CSB-E06828p             96T Guinea pig insulin (INS) ELISA kit            CSB-EL011742GU          96T Fish Insulin,INS ELISA Kit                    CSB-E12123Fh            96T Human Insulin,INS ELISA Kit                   CSB-E05069h             96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit   CSB-E05076r      96T Bovine Insulin ELISA kit                      CSB-E11993B             96T Rat Insulin,INS ELISA Kit                     CSB-E05070r             96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit      CSB-E05074m             96T Sheep Insulin(INS) ELISA Kit                  CSB-E17044Sh            96T Monkey Insulin,INS ELISA Kit                  CSB-E10047Mo            96T Horse insulin (INS) ELISA kit                 CSB-EL011742HO          96T Goat Insulin(INS)ELISA Kit                    CSB-E17653G             96T Cat insulin (INS) ELISA kit                   CSB-EL011742CA          96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05075h     96T

产品名称:猪胰岛素(INS)ELISA Kit 英文名称:Pig Insulin,INS ELISA Kit 产品类型： ELISA Kit 货号： CSB-E06828p 价格： 3800 规格： 96T 种属： Pig 待测物名称： insulin 缩写： INS 样本类型： serum, plasma, tissue homogenates 检测范围： 2 μIU/ml-400 μIU/ml 灵敏度： 1 μIU/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 产品名称                                         货号                 规格 Human Free Thyroxine,FT4 ELISA Kit            CSB-E05078h             96T Chicken Insulin ELISA kit                     CSB-E13293C             96T Dog Insulin,INS ELISA Kit                     CSB-E11327c             96T rabbit Insulin,INS ELISA Kit                  CSB-E06992Rb            96T Rat Free Thyroxine,FT4 ELISA Kit              CSB-E05079r             96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05077m     96T Pig Insulin,INS ELISA Kit                     CSB-E06828p             96T Guinea pig insulin (INS) ELISA kit            CSB-EL011742GU          96T Fish Insulin,INS ELISA Kit                    CSB-E12123Fh            96T Human Insulin,INS ELISA Kit                   CSB-E05069h             96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit   CSB-E05076r      96T Bovine Insulin ELISA kit                      CSB-E11993B             96T Rat Insulin,INS ELISA Kit                     CSB-E05070r             96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit      CSB-E05074m             96T Sheep Insulin(INS) ELISA Kit                  CSB-E17044Sh            96T Monkey Insulin,INS ELISA Kit                  CSB-E10047Mo            96T Horse insulin (INS) ELISA kit                 CSB-EL011742HO          96T Goat Insulin(INS)ELISA Kit                    CSB-E17653G             96T Cat insulin (INS) ELISA kit                   CSB-EL011742CA          96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05075h     96T

产品名称:小鼠胰岛素(INS)ELISA Kit 英文名称:Mouse Insulin,INS ELISA Kit 产品类型： ELISA Kit 货号： CSB-E05071m 价格： 3200 规格： 96T 种属： Mouse 待测物名称： Insulin,INS 缩写： INS 样本类型： serum, plasma, cell culture supernates, tissue homogenates, cell lysates 检测范围： 15.6 nIU/ml -1000 nIU/ml 灵敏度： 3.9 nIU/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 产品名称                                         货号                 规格 Human Free Thyroxine,FT4 ELISA Kit            CSB-E05078h             96T Chicken Insulin ELISA kit                     CSB-E13293C             96T Dog Insulin,INS ELISA Kit                     CSB-E11327c             96T rabbit Insulin,INS ELISA Kit                  CSB-E06992Rb            96T Rat Free Thyroxine,FT4 ELISA Kit              CSB-E05079r             96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05077m     96T Pig Insulin,INS ELISA Kit                     CSB-E06828p             96T Guinea pig insulin (INS) ELISA kit            CSB-EL011742GU          96T Fish Insulin,INS ELISA Kit                    CSB-E12123Fh            96T Human Insulin,INS ELISA Kit                   CSB-E05069h             96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit   CSB-E05076r      96T Bovine Insulin ELISA kit                      CSB-E11993B             96T Rat Insulin,INS ELISA Kit                     CSB-E05070r             96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit      CSB-E05074m             96T Sheep Insulin(INS) ELISA Kit                  CSB-E17044Sh            96T Monkey Insulin,INS ELISA Kit                  CSB-E10047Mo            96T Horse insulin (INS) ELISA kit                 CSB-EL011742HO          96T Goat Insulin(INS)ELISA Kit                    CSB-E17653G             96T Cat insulin (INS) ELISA kit                   CSB-EL011742CA          96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit  CSB-E05075h     96T

产品名称:兔子胰岛素(INS)ELISA Kit 英文名称:rabbit Insulin,INS ELISA Kit 产品类型： ELISA Kit 货号： CSB-E06992Rb 价格： 3800 规格： 96T 种属： Rabbit 待测物名称： insulin 缩写： INS 样本类型： serum, plasma, tissue homogenates 检测范围： 8 μIU/ml-140 μIU/ml 灵敏度： 5 μIU/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 产品名称                            货号                规格  Chicken Insulin ELISA kit       CSB-E13293C            96T Dog Insulin,INS ELISA Kit        CSB-E11327c            96T Pig Insulin,INS ELISA Kit        CSB-E06828p            96T Guinea pig insulin (INS) ELISA kit  CSB-EL011742GU      96T Fish Insulin,INS ELISA Kit       CSB-E12123Fh           96T Mouse Insulin,INS ELISA Kit      CSB-E05071m            96T Human Insulin,INS ELISA Kit      CSB-E05069h            96T Bovine Insulin ELISA kit         CSB-E11993B            96T Rat Insulin,INS ELISA Kit        CSB-E05070r            96T Sheep Insulin(INS) ELISA Kit     CSB-E17044Sh           96T Monkey Insulin,INS ELISA Kit     CSB-E10047Mo           96T Horse insulin (INS) ELISA kit    CSB-EL011742HO         96T Goat Insulin(INS)ELISA Kit       CSB-E17653G            96T Cat insulin (INS) ELISA kit      CSB-EL011742CA         96T 抗体 产品名称                               货号             规格 Rabbit anti-human Insulin(INS) polyclonal Antibody CSB-PA011742GA01HU 150ul 蛋白 产品名称                                                规格 Recombinant Verasper moseri Insulin(ins)                1mg Recombinant Ornithorhynchus anatinus Insulin(INS)       1mg Recombinant Aplysia californica Insulin(PIN)            1mg Recombinant Pantodon buchholzi Insulin(ins)             1mg Recombinant Spermophilus tridecemlineatus Insulin(INS)  1mg

产品名称:人胰岛素(INS)ELISA Kit 英文名称:Human Insulin,INS ELISA Kit 产品类型： ELISA Kit 货号： CSB-E05069h 价格： 3600 规格： 96T 种属： Human 待测物名称： insulin 缩写： INS 样本类型： serum, plasma, cell culture supernates, tissue homogenates 检测范围： 8μIU/ml-140μIU/ml 灵敏度： 5μIU/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IN......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INS bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Human INS. No significant cross-reactivity or interference between Human INS and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the INS concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   产品名称                             货号             规格 Chicken Insulin ELISA kit       CSB-E13293C           96T Dog Insulin,INS ELISA Kit       CSB-E11327c           96T rabbit Insulin,INS ELISA Kit    CSB-E06992Rb          96T Pig Insulin,INS ELISA Kit       CSB-E06828p           96T Guinea pig insulin (INS) ELISA kit  CSB-EL011742GU    96T Fish Insulin,INS ELISA Kit     CSB-E12123Fh           96T Mouse Insulin,INS ELISA Kit     CSB-E05071m           96T Bovine Insulin ELISA kit        CSB-E11993B           96T Rat Insulin,INS ELISA Kit       CSB-E05070r           96T Sheep Insulin(INS) ELISA Kit    CSB-E17044Sh          96T Monkey Insulin,INS ELISA Kit    CSB-E10047Mo          96T Horse insulin (INS) ELISA kit   CSB-EL011742HO        96T Goat Insulin(INS)ELISA Kit      CSB-E17653G           96T Cat insulin (INS) ELISA kit     CSB-EL011742CA        96T 抗体 产品名称                       货号                   规格 Rabbit anti-human Insulin(INS) polyclonal Antibody CSB-PA011742GA01HU 150ul 蛋白 产品名称                                              规格 Recombinant Verasper moseri Insulin(ins)              1mg Recombinant Ornithorhynchus anatinus Insulin(INS)     1mg Recombinant Aplysia californica Insulin(PIN)          1mg Recombinant Pantodon buchholzi Insulin(ins)           1mg Recombinant Spermophilus tridecemlineatus Insulin(INS) 1mg

产品名称:大鼠胰岛素(INS)ELISA Kit 英文名称:Rat Insulin,INS ELISA Kit 产品类型： ELISA Kit 货号： CSB-E05070r 价格： 3800 规格： 96T 种属： Rat 待测物名称： Insulin,INS 缩写： INS 样本类型： serum, plasma, cell culture supernates 检测范围： 15.6 nIU/ml-1000 nIU/ml 灵敏度： 3.9 nIU/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 产品名称                               货号          规格 Human Free Thyroxine,FT4 ELISA Kit  CSB-E05078h      96T Chicken Insulin ELISA kit           CSB-E13293C      96T Dog Insulin,INS ELISA Kit           CSB-E11327c      96T rabbit Insulin,INS ELISA Kit        CSB-E06992Rb     96T Rat Free Thyroxine,FT4 ELISA Kit    CSB-E05079r      96T

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产品名称:牛肾上腺髓质素(ADM)ELISA kit 英文名称:Bovine adrenomedullin,ADM ELISA kit 产品类型： ELISA Kit 货号： CSB-EL001370BO 价格： 4200 规格： 96T 种属： Bovine 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.―特异性： This assay has high sensitivity and excellent specificity for detection of Bovine ADM. No significant cross-reactivity or interference between Bovine ADM and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称        货号      规格 Human adrenomedullin,ADM ELISA Kit  CSB-E09146h 96T Dog adrenomedullin,ADM ELISA Kit   CSB-E09836c 96T Fish adrenomedullin (ADM) ELISA kit  CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit  CSB-E10061m 96T Rat adrenomedullin,ADM ELISA Kit  CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit  CSB-E09837Rb 96T Pig adrenomedullin (ADM) ELISA kit  CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit CSB-E04843Ch 96T 蛋白 产品名称           规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg

产品名称:犬肾上腺髓质素(ADM)ELISA Kit 英文名称:Dog adrenomedullin,ADM ELISA Kit 产品类型： ELISA Kit  货号： CSB-E09836c 价格： 4200 规格： 96T 种属： Dog 样本类型： serum, plasma, tissue homogenates, cell culture supernates 检测范围： 15.6 pg/ml-1000 pg/ml 灵敏度： 3.9 pg/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Dog ADM. No significant cross-reactivity or interference between Dog ADM and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称        货号      规格 Human adrenomedullin,ADM ELISA Kit  CSB-E09146h 96T Bovine adrenomedullin (ADM) ELISA kit  CSB-EL001370BO 96T Fish adrenomedullin (ADM) ELISA kit  CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit  CSB-E10061m 96T Rat adrenomedullin,ADM ELISA Kit  CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit  CSB-E09837Rb 96T Pig adrenomedullin (ADM) ELISA kit  CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit CSB-E04843Ch 96T 蛋白 产品名称           规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg

产品名称:大鼠肾上腺髓质素(ADM)ELISA Kit 英文名称:Rat adrenomedullin,ADM ELISA Kit 产品类型： ELISA Kit 货号： CSB-E10060r 价格： 3900 规格： 96T 种属： Rat 检测范围： 0.312 nmol/ml-20 nmol/ml 灵敏度： 0.078 nmol/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Rat ADM. No significant cross-reactivity or interference between Rat ADM and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称            货号                规格 Dog adrenomedullin,ADM ELISA Kit     CSB-E09836c 96T Human adrenomedullin,ADM ELISA Kit   CSB-E09146h 96T Bovine adrenomedullin,ADM ELISA kit  CSB-EL001370BO 96T  Fish adrenomedullin,ADM ELISA kit    CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit   CSB-E10061m 96T Rabbit adrenomedullin,ADM ELISA Kit  CSB-E09837Rb 96T Pig adrenomedullin,ADM ELISA Kit     CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit  CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit  CSB-E04843Ch 96T 蛋白 产品名称           规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg

产品名称:人肾上腺髓质素(ADM)ELISA Kit 英文名称:Human adrenomedullin,ADM ELISA Kit 产品类型： ELISA Kit  货号： CSB-E09146h 价格： 3600 规格： 96T 种属： Human 样本类型： serum, plasma, tissue homogenates 检测范围： 0.312 pg/ml-20 pg/ml 灵敏度： 0.078 pg/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450nm 用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.―特异性： This assay has high sensitivity and excellent specificity for detection of Human ADM. No significant cross-reactivity or interference between Human ADM and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称          货号           规格 Dog adrenomedullin,ADM ELISA Kit  CSB-E09836c 96T Bovine adrenomedullin (ADM) ELISA kit  CSB-EL001370BO 96T Fish adrenomedullin (ADM) ELISA kit CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit  CSB-E10061m 96T Rat adrenomedullin,ADM ELISA Kit    CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit  CSB-E09837Rb 96T Pig adrenomedullin (ADM)ELISA kit    CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit  CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit  CSB-E04843Ch 96T 蛋白 产品名称          规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg

产品名称:小鼠肾上腺髓质素(ADM)ELISA Kit 英文名称:Mouse adrenomedullin,ADM ELISA Kit 产品类型： ELISA Kit 货号： CSB-E10061m 价格： 3800 规格： 96T 种属： Mouse 检测范围： 4.7 pg/ml-300 pg/ml 灵敏度： 1.2 pg/ml 反应时间： 1-5h 所需样本体积： 50-100ul 检测波长： 450 nm 标准曲线：  用途： For research use only. Not for diagnostic use. 原理： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......＋This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性： This assay has high sensitivity and excellent specificity for detection of Mouse ADM. No significant cross-reactivity or interference between Mouse ADM and analogues was observed. 精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......＋Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......＋Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......＋Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   产品名称     货号      规格 Dog adrenomedullin,ADM ELISA Kit     CSB-E09836c 96T Human adrenomedullin,ADM ELISA Kit   CSB-E09146h 96T Bovine adrenomedullin (ADM) ELISA kit  CSB-EL001370BO 96T Fish adrenomedullin (ADM) ELISA kit  CSB-EL001370FI 96T Rat adrenomedullin,ADM ELISA Kit    CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit   CSB-E09837Rb 96T Pig adrenomedullin (ADM) ELISA kit   CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit   CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit  CSB-E04843Ch 96T 蛋白 产品名称       规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg Recombinant Mouse ADM(Adm) 1mg

产品名称： 人肌酸激酶同工酶BB(CK-BB)ELISA Kit    英文名称： Human creatine kinase BB isoenzyme,CK-BB ELISA kit    货号： CSB-EQ027522HU    价格： 3600    规格： 96T    种属： Human    待测物名称： creatine kinase BB isoenzyme (CK-BB)    缩写： CK-BB    样本类型： serum, plasma, tissue homogenates    检测范围： 3.12 mU/ml-200 mU/ml    灵敏度： 0.78mU/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450nm    用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  兔肌酸激酶同工酶BB(CK-BB)ELISA Kit  Rabbit creatine kinase BB isoenzyme (CK-BB) ELISA kit  CSB-EQ027522RB  96T            小鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit  Mouse creatine kinase BB isoenzyme (CK-BB) ELISA kit  CSB-EQ027522MO  96T            大鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit  Rat creatine kinase BB isoenzyme (CK-BB) ELISA kit  CSB-EQ027522RA  96T            牛肌酸激酶同工酶BB(CK-BB)ELISA Kit  Bovine Creatine Kinase BB Isoenzymes,CK-BB ELISA Kit  CSB-E14138B  96T

产品名称： 牛肌酸激酶同工酶BB(CK-BB)ELISA Kit    英文名称： Bovine Creatine Kinase BB Isoenzymes,CK-BB ELISA Kit    货号： CSB-E14138B    规格： 96T    种属： Bovine    待测物名称： Creatine Kinase BB Isoenzymes,CK-BB    缩写： CK-BB    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  大鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit  Rat creatine kinase BB isoenzyme (CK-BB) ELISA kit  CSB-EQ027522RA  96T            人肌酸激酶同工酶BB(CK-BB)ELISA Kit  Human creatine kinase BB isoenzyme (CK-BB) ELISA kit  CSB-EQ027522HU  96T            牛凝血酶抗凝血酶Ⅲ复合物(TAT-III)ELISA Kit  Bovine Thrombin-antithrombin III Complex, TAT-III ELISA Kit  CSB-E14133B  96T            小鼠幽门螺旋杆菌抗体(IgM)ELISA Kit  Mouse Helicobacter pylori antibody(IgM)ELISA Kit  CSB-E14131m  96T            小鼠幽门螺旋杆菌抗体(IgG)ELISA Kit  Mouse Helicobacter pylori antibody(IgG)ELISA Kit  CSB-E14132m  96T            牛内皮型一氧化氮合成酶(eNOS)ELISA Kit  Bovine Endothelial nitric oxide synthase,eNOS ELISA Kit  CSB-E14137B  96T            牛诱导型一氧化氮合成酶(iNOS)ELISA Kit  Bovine Inducible Nitric Oxide Synthase,iNOS ELISA Kit  CSB-E14135B  96T            牛神经型一氧化氮合成酶(nNOS/NOS1)ELISA Kit  Bovine Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit  CSB-E14136B  96T            牛肌酸激酶同工酶MB(CK-MB)ELISA Kit  Bovine Creatine Kinase MB Isoenzyme,CK-MB ELISA Kit  CSB-E14139B  96T            大鼠激动异构酶/细胞分裂素MB(CKMB)ELISA Kit  Rat cytokinin MB,CKMB ELISA Kit  CSB-E14130r  96T

产品名称： 大鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit    英文名称： Rat creatine kinase BB isoenzyme,CK-BB ELISA kit    货号： CSB-EQ027522RA    规格： 96T    种属： Rat    待测物名称： creatine kinase BB isoenzyme (CK-BB)    缩写： CK-BB    样本类型： serum, plasma, tissue homogenates    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  人肌酸激酶同工酶BB(CK-BB)ELISA Kit  Human creatine kinase BB isoenzyme (CK-BB) ELISA kit  CSB-EQ027522HU  96T            牛肌酸激酶同工酶BB(CK-BB)ELISA Kit  Bovine Creatine Kinase BB Isoenzymes,CK-BB ELISA Kit  CSB-E14138B  96T

产品名称： 小鼠肾损伤分子1(Kim-1)ELISA Kit    英文名称： Mouse Kidney injury molecule 1,Kim-1 ELISA Kit    别名： HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1    货号： CSB-E08809m    规格： 96T    种属： Mouse    待测物名称： hepatitis A virus cellular receptor 1    缩写： HAVCR1    检测范围： 0.94 ng/ml-60 ng/ml    灵敏度： 0.23 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Mouse HAVCR1. No significant cross-reactivity or interference between Mouse HAVCR1 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  大鼠肾损伤分子1(Kim-1)ELISA Kit  Rat Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08808r  96T            人肾损伤分子1(Kim-1)ELISA Kit  Human Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08807h  96T            猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit  Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144PI  96T            猴肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit  Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144RH  96T           蛋白  产品名称 货号 规格 蛋白来源  Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1)  1mg            Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1)  1mg            Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1)  1mg

产品名称： 大鼠肾损伤分子1(Kim-1)ELISA Kit    英文名称： Rat Kidney injury molecule 1,Kim-1 ELISA Kit    别名： HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1    货号： CSB-E08808r    规格： 96T    种属： Rat    待测物名称： hepatitis A virus cellular receptor 1    缩写： HAVCR1    样本类型： serum, plasma, urine    检测范围： 0.312 ng/ml-20 ng/ml    灵敏度： 0.078 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Rat HAVCR1. No significant cross-reactivity or interference between Rat HAVCR1 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.     产品名称 英文名称 货号 规格  人肾损伤分子1(Kim-1)ELISA Kit  Human Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08807h  96T            猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit  Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144PI  96T            小鼠肾损伤分子1(Kim-1)ELISA Kit  Mouse Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08809m  96T            猴肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit  Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144RH  96T           蛋白  产品名称 货号 规格 蛋白来源  Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1)  1mg            Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1)  1mg            Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1)  1mg

产品名称： 人肾损伤分子1(Kim-1)ELISA Kit    英文名称： Human Kidney injury molecule 1,Kim-1 ELISA Kit    别名： HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1    货号： CSB-E08807h    规格： 96T    种属： Human    待测物名称： hepatitis A virus cellular receptor 1    缩写： HAVCR1    蛋白功能1： Immunity    蛋白功能3： Host-virus interaction    样本类型： serum, urine, tissue homogenates    检测范围： 0.312 ng/ml-20 ng/ml    灵敏度： 0.078 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Human HAVCR1. No significant cross-reactivity or interference between Human HAVCR1 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  大鼠肾损伤分子1(Kim-1)ELISA Kit  Rat Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08808r  96T            猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit  Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144PI  96T            小鼠肾损伤分子1(Kim-1)ELISA Kit  Mouse Kidney injury molecule 1,Kim-1 ELISA Kit  CSB-E08809m  96T            猴肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit  Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit  CSB-EL010144RH  96T           蛋白  产品名称 货号 规格 蛋白来源  Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1)  CSB-EP010144DSU  1mg  E.coli            Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1)  CSB-YP010144DSU  1mg  Yeast            Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1)  CSB-EP010144RA  1mg  E.coli            Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1)  CSB-YP010144RA  1mg  Yeast

产品名称： 犬肾损伤分子1(Kim-1)ELISA Kit    英文名称： Canine kidney injury molecule 1,Kim-1 ELISA Kit    货号： CSB-E13035c    规格： 96T    种属： Dog    待测物名称： kidney injury molecule 1(Kim-1)    缩写： Kim-1    样本类型： serum, plasma, tissue homogenates    检测范围： 0.312 ng/ml-20 ng/ml    灵敏度： 0.078ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm      用途： For research use only. Not for diagnostic use.    相关产品：   产品名称 英文名称 货号 规格  大鼠不对称二甲基精氨酸(ADMA)ELISA Kit  Rat asymmetrical dimethylarginine(ADMA) ELISA Kit  CSB-E13039r  96T            大鼠一氧化氮(NO)ELISA Kit  Rat nitric oxide (NO） ELISA Kit  CSB-E13034r  96T            大鼠肿瘤坏死因子γ（TNF-γ）ELISA Kit  Rat tumor necrosis factor γ (TNF-γ) ELISA Kit  CSB-E13033r  96T            鸡白介素6受体(IL-6R)ELISA Kit  Chicken Interleukin 6 receptor (IL-6R) ELISA Kit  CSB-E13030C  96T            豚鼠白介素18(IL-18)ELISA Kit  Guinea pig Interleukin 18(IL-18） ELISA Kit  CSB-E13032Gu  96T            鸡白介素15(IL-15)ELISA Kit  Chicken Interleukin 15(IL-15) ELISA Kit  CSB-E13031C  96T            大鼠血红蛋白ELISA Kit  Rat Hemoglobin (Hb) ELISA Kit  CSB-E13036r  96T            人CD82分子ELISA Kit  Human cluster Of differentiation 82 (CD82) ELISA Kit  CSB-E13037h  96T

产品名称： 小鼠白介素1β (IL-1β)ELISA Kit    英文名称： Mouse Interleukin 1β,IL-1β ELISA Kit    别名： IL-1, IL1-BETA, IL1F2    货号： CSB-E08054m    价格： 3200    规格： 96T    种属： Mouse    待测物名称： interleukin 1, beta    缩写： IL1B    样本类型： serum, plasma, cell culture supernates, tissue homogenates    检测范围： 31.25 pg/ml-2000 pg/ml    灵敏度： 7.81 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1B bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Mouse IL1B. No significant cross-reactivity or interference between Mouse IL1B and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL1B concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  绵羊白介素1β(IL-1β)ELISA Kit  Sheep Interleukin 1β,IL-1β ELISA Kit  CSB-E10115Sh  96T            大鼠白介素1β前体(pro-IL1B)ELISA Kit  Rat pro interleukin 1 beta (pro-IL1B) ELISA Kit  CSB-ET011614RA  96T            犬白介素1β(IL-1β) ELISA Kit  Dog Interleukin 1β (IL- 1β) ELISA Kit  CSB-E13836c  96T            兔子白介素1β (IL-1β)ELISA Kit  Rabbit Interleukin 1β,IL-1β ELISA Kit  CSB-E06900Rb  96T            牛白介素1β(IL-1β) ELISA kit  Bovine Interleukin 1β (IL- 1β) ELISA kit  CSB-E12986B  96T            豚鼠白介素1β(IL1B) ELISA kit  Guinea pig Interleukin-1 beta(IL1B) ELISA kit  CSB-EL011614GU  96T            山羊白介素1β(IL-1β)ELISA Kit  Goat Interleukin 1β(IL-1β) ELISA Kit  CSB-E16568G  96T            人白介素1β (IL-1β)ELISA Kit  Human Interleukin 1β,IL-1β ELISA Kit  CSB-E08053h  96T            马白介素1β(IL-1β)ELISA Kit  Horse Interleukin 1β(IL-1β)ELISA Kit  CSB-E16635Hs  96T            人白介素1β前体(pro-IL1B)ELISA Kit  Human pro interleukin 1 beta (pro-IL1B) ELISA Kit  CSB-ET011614HU  96T            大鼠白介素1β (IL-1β)ELISA Kit  Rat Interleukin 1β,IL-1β ELISA Kit  CSB-E08055r  96T            小鼠白介素1β前体(pro-IL1B)ELISA Kit  Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit  CSB-ET011614MO  96T            猪白介素1β (IL-1β)ELISA Kit  Pig Interleukin 1β,IL-1β ELISA Kit  CSB-E06782p  96T            Monkey Interleukin-1 beta(IL1B) ELISA kit  Monkey Interleukin-1 beta(IL1B) ELISA kit  CSB-EL011614RH  96T            Cat Interleukin-1 beta(IL1B) ELISA kit  Cat Interleukin-1 beta(IL1B) ELISA kit  CSB-EL011614CA  96T           抗体  产品名称 货号 规格  Rabbit anti-human interleukin 1, beta polyclonal Antibody  CSB-PA011614GA01HU  150ul         蛋白  产品名称 货号 规格 蛋白来源  Recombinant Mustela putorius furo Interleukin-1 beta(IL1B)  1mg            Recombinant Cercocebus atys Interleukin-1 beta(IL1B)  1mg            Recombinant Cervus elaphus Interleukin-1 beta(IL1B)  1mg            Recombinant Macaca nemestrina Interleukin-1 beta(IL1B)  1mg            Recombinant Macaca fascicularis Interleukin-1 beta(IL1B)  1mg            Recombinant Human Interleukin-1 beta(IL1B)  1mg            Recombinant Bovine Interleukin-1 beta(IL1B)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interleukin-1 beta(IL1B)  1mg            Recombinant Cat Interleukin-1 beta(IL1B)  1mg            Recombinant Pig Interleukin-1 beta(IL1B)  1mg            Recombinant Sheep Interleukin-1 beta(IL1B)  1mg            Recombinant Rabbit Interleukin-1 beta(IL1B)  1mg            Recombinant Mouse Interleukin-1 beta(Il1b)  1mg            Recombinant Goat Interleukin-1 beta(IL1B)  1mg

产品名称： 小鼠白介素1α(IL-1α)ELISA Kit    英文名称： Mouse Interleukin 1α,IL-1α ELISA kit    别名： IL-1A, IL1, IL1-ALPHA, IL1F1, IL1A (IL1F1)    货号： CSB-E04621m    价格： 3200    规格： 96T    种属： Mouse    待测物名称： interleukin 1, alpha    缩写： IL1A    蛋白功能1： Immunity    蛋白功能3： Inflammatory response    样本类型： serum, plasma, tissue homogenates, cell culture supernates    检测范围： 1.56 pg/ml-100 pg/ml    灵敏度： 0.39 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1A bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Mouse IL1A. No significant cross-reactivity or interference between Mouse IL1A and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL1A concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  Bovine Interleukin-1 alpha(IL1A) ELISA kit  Bovine Interleukin-1 alpha(IL1A) ELISA kit  CSB-EL011613BO  96T            大鼠白介素1α(IL-1α)ELISA kit  Rat Interleukin 1α ,IL-1α ELISA kit  CSB-E04622r  96T            兔子白介素1α (IL-1α)ELISA Kit  Rabbit Interleukin 1α,IL-1α ELISA Kit  CSB-E13154Rb  96T            绵羊白介素1α(IL-1α)ELISA Kit  Sheep Interleukin 1α(IL-1α)ELISA Kit  CSB-E15096Sh  96T            猪白介素1α(IL-1α)ELISA kit  Pig Interleukin 1α ,IL-1α ELISA kit  CSB-E09725p  96T            犬白介素1α(IL1A)ELISA kit  Dog Interleukin-1 alpha(IL1A) ELISA kit  CSB-EL011613DO  96T            人白介素1α(IL-1α )ELISA Kit  Human Interleukin 1α,IL-1α ELISA Kit  CSB-E04620h  96T            猴白介素1a(IL1A)ELISA Kit  Monkey Interleukin-1 alpha (IL1A) ELISA kit  CSB-E10041Mo  96T            山羊白介素1α(IL-1α)ELISA Kit  Goat Interleukin 1α(IL-1α) ELISA Kit  CSB-E15099G  96T            Horse Interleukin-1 alpha(IL1A) ELISA kit  Horse Interleukin-1 alpha(IL1A) ELISA kit  CSB-EL011613HO  96T            Guinea pig Interleukin-1 alpha(IL1A) ELISA kit  Guinea pig Interleukin-1 alpha(IL1A) ELISA kit  CSB-EL011613GU  96T            Cat Interleukin-1 alpha(IL1A) ELISA kit  Cat Interleukin-1 alpha(IL1A) ELISA kit  CSB-EL011613CA  96T           抗体  产品名称 货号 规格  Rabbit anti-human interleukin 1, alpha polyclonal Antibody  CSB-PA011613GA01HU  150ul         蛋白  产品名称 货号 规格 蛋白来源  Recombinant Cercocebus atys Interleukin-1 alpha(IL1A)  1mg            Recombinant Rat Interleukin-1 alpha(Il1a)  1mg            Recombinant Macaca fascicularis Interleukin-1 alpha(IL1A)  1mg            Recombinant Dog Interleukin-1 alpha(IL1A)  1mg            Recombinant Cat Interleukin-1 alpha(IL1A)  1mg            Recombinant Rabbit Interleukin-1 alpha(IL1A)  1mg            Recombinant Bovine Interleukin-1 alpha(IL1A)  1mg            Recombinant Human Interleukin-1 alpha(IL1A)  1mg            Recombinant Mouse Interleukin-1 alpha(Il1a)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interleukin-1 alpha(IL1A)  1mg            Recombinant Pig Interleukin-1 alpha(IL1A)  1mg            Recombinant Goat Interleukin-1 alpha(IL1A)  1mg

产品名称： 人转化生长因子β1(TGF-β1)ELISA kit    英文名称： Human Transforming Growth factor β1,TGF-β1 ELISA kit    别名： CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein|latency-associated peptide    货号： CSB-E04725h    规格： 96T    种属： Human    待测物名称： transforming growth factor, beta 1    缩写： TGFB1    样本类型： serum, plasma, cell culture supernates    检测范围： 0.8 ng/ml-50 ng/ml    灵敏度： 0.2 ng/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGFB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Human TGFB1. No significant cross-reactivity or interference between Human TGFB1 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TGFB1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.    相关产品：   产品名称 英文名称 货号 规格  绵羊转化生长因子β1(TGFB1)ELISA kit  Sheep Transforming growth factor beta-1(TGFB1) ELISA kit  CSB-EL023446SH  96T            大鼠转化生长因子β1(TGF-β1)ELISA kit  Rat Transforming Growth Factor β1(TGF-β1) ELISA kit  CSB-E04727r  96T            鱼转化生长因子β1(TGFB1) ELISA kit  Fish transforming growth factor, beta 1 (TGFB1) ELISA kit  CSB-EL023446FI  96T            牛转化生长因子β1(TGF-β1)ELISA Kit  Bovine transforming growth factor β1,TGF-β1 ELISA Kit  CSB-E14208B  96T            猪转化生长因子β1(TGF-β1)ELISA Kit  Pig transforming growth factor β1(TGF-β1)ELISA Kit  CSB-E06843p  96T            小鼠转化生长因子β1(TGF-β1)ELISA Kit  Mouse Transforming Growth factor β1,TGF-β1 ELISA kit  CSB-E04726m  96T            Horse Transforming growth factor beta-1(TGFB1) ELISA kit  Horse Transforming growth factor beta-1(TGFB1) ELISA kit  CSB-EL023446HO  96T            豚鼠转化生长因子β1(TGF-β1)ELISA Kit  guinea pig transforming growth factor β1,TGF-β1 ELISA Kit  CSB-E06773Gu  96T            犬转移生长因子β1(TGF-β1)ELISA Kit  Dog Transforming growth factor β1,TGF-β1 ELISA Kit  CSB-E04796c  96T            鸡转化生长因子β1(TGF-β1)ELISA kit  Chicken Transforming Growth factor β1,TGF-β1 ELISA kit  CSB-E09875Ch  96T           抗体  产品名称 货号 规格  Rabbit anti-human transforming growth factor, beta 1 polyclonal Antibody  CSB-PA023446GA01HU  150ul         蛋白  产品名称 货号 规格 蛋白来源  Recombinant Oncorhynchus mykiss Transforming growth factor beta-1(tgfb1)  CSB-EP023446OEI  1mg  E.coli            Recombinant Oncorhynchus mykiss Transforming growth factor beta-1(tgfb1)  CSB-YP023446OEI  1mg  Yeast            Recombinant Chlorocebus aethiops Transforming growth factor beta-1(TGFB1)  CSB-EP023446DSU  1mg  E.coli            Recombinant Chlorocebus aethiops Transforming growth factor beta-1(TGFB1)  CSB-YP023446DSU  1mg  Yeast            Recombinant Xenopus laevis Transforming growth factor beta-1(tgfb1)  CSB-EP023446XBE  1mg  E.coli            Recombinant Xenopus laevis Transforming growth factor beta-1(tgfb1)  CSB-YP023446XBE  1mg  Yeast            Recombinant Horse Transforming growth factor beta-1(TGFB1)  CSB-EP023446HO  1mg  E.coli            Recombinant Horse Transforming growth factor beta-1(TGFB1)  CSB-YP023446HO  1mg  Yeast            Recombinant Chicken Transforming growth factor beta-1(TGFB1)  CSB-EP023446CH  1mg  E.coli            Recombinant Chicken Transforming growth factor beta-1(TGFB1)  CSB-YP023446CH  1mg  Yeast            Recombinant Pig Transforming growth factor beta-1(TGFB1)  CSB-EP023446PI  1mg  E.coli            Recombinant Pig Transforming growth factor beta-1(TGFB1)  CSB-YP023446PI  1mg  Yeast            Recombinant Human Transforming growth factor beta-1(TGFB1)  CSB-EP023446HU  1mg  E.coli            Recombinant Human Transforming growth factor beta-1(TGFB1)  CSB-YP023446HU  1mg  Yeast            Recombinant Mouse Transforming growth factor beta-1(Tgfb1)  CSB-EP023446MO  1mg  E.coli            Recombinant Mouse Transforming growth factor beta-1(Tgfb1)  CSB-YP023446MO  1mg  Yeast            Recombinant Dog Transforming growth factor beta-1(TGFB1)  CSB-EP023446DO  1mg  E.coli            Recombinant Dog Transforming growth factor beta-1(TGFB1)  CSB-YP023446DO  1mg  Yeast            Recombinant Sheep Transforming growth factor beta-1(TGFB1)  CSB-EP023446SH  1mg  E.coli            Recombinant Sheep Transforming growth factor beta-1(TGFB1)  CSB-YP023446SH  1mg  Yeast            Recombinant Bovine Transforming growth factor beta-1(TGFB1)  CSB-EP023446BO  1mg  E.coli            Recombinant Bovine Transforming growth factor beta-1(TGFB1)  CSB-YP023446BO  1mg  Yeast            Recombinant Rat Transforming growth factor beta-1(Tgfb1)  CSB-EP023446RA  1mg  E.coli            Recombinant Rat Transforming growth factor beta-1(Tgfb1)  CSB-YP023446RA  1mg  Yeast

 产品名称： 猪口蹄疫抗体(IgM)ELISA Kit    英文名称： Pig Antibody against Foot and Mouth Disease(FMDV)IgM ELISA kit    货号： CSB-E12768p    价格： 5000    规格： 192T    种属： Pig    待测物名称： Antibody against Foot and Mouth Disease(FMDV) IgM    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：    产品名称 英文名称 货号 规格  猪瘟抗体(IgG) ELISA Kit  Classical Swine Fever Virus Antibody(IgG) ELISA Kit  CSB-E12765p  192T            犬肾上腺素(EPI)ELISA Kit  Canine Epinephrine/Adrenaline,EPI ELISA Kit  CSB-E12761c  96T            犬髓过氧化物酶(MPO)ELISA Kit  Dog Myeloperoxidase (MPO) ELISA kit  CSB-E12762c  96T            猪口蹄疫抗体(IgG) ELISA Kit  Pig Foot and Mouth Disease Virus Antibody (IgG) ELISA Kit  CSB-E12767p  192T            猪瘟抗体(IgM) ELISA Kit  Anti-CSFV Antibody (IgM) ELISA Kit  CSB-E12766p  192T            犬促肾上皮质激素释放激素(CRH)ELISA Kit  Dog corticotropin releasing hormone,CRH ELISA Kit  CSB-E12763c  96T            大鼠P0蛋白 ELISA kit  Rat myelin protein zero (p0) ELISA Kit  CSB-E12769r  96T            犬皮质酮/肾上腺酮(CORT)ELISA Kit  Canine Corticosterone,CORT ELISA Kit  CSB-E12760c  96T

 产品名称： 猪口蹄疫抗体(IgG)ELISA Kit    英文名称： Pig Foot and Mouth Disease Virus Antibody(IgG)ELISA Kit    货号： CSB-E12767p    价格： 5000    规格： 192T    种属： Pig    待测物名称： Antibody against Foot and Mouth Disease(FMDV) IgG    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    相关产品：    产品名称 英文名称 货号 规格  猪瘟抗体(IgG) ELISA Kit  Classical Swine Fever Virus Antibody(IgG) ELISA Kit  CSB-E12765p  192T            犬肾上腺素(EPI)ELISA Kit  Canine Epinephrine/Adrenaline,EPI ELISA Kit  CSB-E12761c  96T            犬髓过氧化物酶(MPO)ELISA Kit  Dog Myeloperoxidase (MPO) ELISA kit  CSB-E12762c  96T            猪口蹄疫抗体(IgM) ELISA Kit  Pig Antibody against Foot and Mouth Disease(FMDV) IgM ELISA kit  CSB-E12768p  192T            猪瘟抗体(IgM) ELISA Kit  Anti-CSFV Antibody (IgM) ELISA Kit  CSB-E12766p  192T            犬促肾上皮质激素释放激素(CRH)ELISA Kit  Dog corticotropin releasing hormone,CRH ELISA Kit  CSB-E12763c  96T            大鼠P0蛋白 ELISA kit  Rat myelin protein zero (p0) ELISA Kit  CSB-E12769r  96T            犬皮质酮/肾上腺酮(CORT)ELISA Kit  Canine Corticosterone,CORT ELISA Kit  CSB-E12760c  96T

 产品名称： 猪蓝耳病毒(PRRSV)抗体(IgG)ELISA kit    英文名称： Pig reproductive and respiratory syndrome virus(PRRSV)antibody(IgG)ELISA kit    货号： CSB-EQ027198PI    价格： 5000    规格： 192T    种属： Pig    待测物名称： reproductive and respiratory syndrome virus(PRRSV)antibody(IgG)    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.

 产品名称： 大鼠白介素6(IL-6)ELISA KIT    英文名称： Rat Interleukin 6,IL-6 ELISA KIT    别名： BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2    货号： CSB-E04640r    价格： 3200    规格： 96T    种属： Rat    待测物名称： interleukin 6 (interferon, beta 2)    缩写： IL6    样本类型： serum, plasma, cell culture supernates, tissue homogenates, cell lysates    检测范围： 0.312 pg/ml-20 pg/ml    灵敏度： 0.078 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm   标准曲线：  原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Rat IL6. No significant cross-reactivity or interference between Rat IL6 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  猪白介素6(IL-6)ELISA Kit  Pig Interleukin 6,IL-6 ELISA Kit  CSB-E06786p  96T            人白介素6(IL-6)ELISA KIT  Human Interleukin 6,IL-6 ELISA KIT  CSB-E04638h  96T            牛白介素6(IL-6)ELISA Kit  Bovine Interleukin 6,IL-6 ELISA Kit  CSB-E12899B  96T            人超敏白介素6(hs-IL6) ELISA kit  Human high sensitivity interleukin 6 (hs-IL6) ELISA kit  CSB-EQ011664HU  96T            绵羊白介素6(IL-6)ELISA Kit  Sheep Interleukin 6,IL-6 ELISA Kit  CSB-E10116Sh  96T            小鼠白介素6(IL-6)ELISA Kit  Mouse Interleukin 6,IL-6 ELISA KIT  CSB-E04639m  96T            猫白介素6(IL-6)ELISA Kit  Cat Interleukin 6(IL-6) ELISA Kit  CSB-E14046Fe  96T            鸡白介素6(IL-6)ELISA Kit  Chicken Interleukin 6,IL-6 ELISA Kit  CSB-E08549Ch  96T            马白介素6(IL-6)ELISA Kit  Horse Interleukin 6(IL-6)ELISA Kit  CSB-E16634Hs  96T            兔子白介素6(IL-6)ELISA Kit  rabbit Interleukin 6,IL-6 ELISA Kit  CSB-E06903Rb  96T            猴白介素6(IL-6)ELISA Kit  Monkey Interleukin 6,IL-6 ELISA Kit  CSB-E10050Mo  96T            山羊白介素6(IL-6)ELISA Kit  Goat Interleukin 6,IL-6 ELISA Kit  CSB-E14360G  96T            犬白介素6(IL-6)ELISA Kit  Dog Interleukin 6,IL-6 ELISA Kit  CSB-E11260c  96T           蛋白   产品名称 货号 规格 蛋白来源  Recombinant Marmota monax Interleukin-6(IL6)  1mg            Recombinant Sparus aurata Interleukin-6(il6)  1mg            Recombinant Mustela putorius furo Interleukin-6(IL6)  1mg            Recombinant Cercocebus atys Interleukin-6(IL6)  1mg            Recombinant Mustela vison Interleukin-6(IL6)  1mg            Recombinant Rat Interleukin-6(Il6)  1mg            Recombinant Macaca fascicularis Interleukin-6(IL6)  1mg            Recombinant Mouse Interleukin-6(Il6)  1mg            Recombinant Human Interleukin-6(IL6)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6)  1mg            Recombinant Cat Interleukin-6(IL6)  1mg            Recombinant Sheep Interleukin-6(IL6)  1mg            Recombinant Pig Interleukin-6(IL6)  1mg            Recombinant Dog Interleukin-6(IL6)  1mg            Recombinant Bovine Interleukin-6(IL6)  1mg

 产品名称： 人白介素6(IL-6)ELISA KIT    英文名称： Human Interleukin 6,IL-6 ELISA KIT    别名： BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2    货号： CSB-E04638h    价格： 3600    规格： 96T    种属： Human    待测物名称： interleukin 6 (interferon, beta 2)    缩写： IL6    样本类型： serum, plasma, cell culture supernates, urine, tissue homogenates    检测范围： 7.8 pg/ml-500 pg/ml    灵敏度： 1.95 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Human IL6. No significant cross-reactivity or interference between Human IL6 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.    相关产品：    产品名称 英文名称 货号 规格  猪白介素6(IL-6)ELISA Kit  Pig Interleukin 6,IL-6 ELISA Kit  CSB-E06786p  96T            牛白介素6(IL-6)ELISA Kit  Bovine Interleukin 6,IL-6 ELISA Kit  CSB-E12899B  96T            人超敏白介素6(hs-IL6) ELISA kit  Human high sensitivity interleukin 6 (hs-IL6) ELISA kit  CSB-EQ011664HU  96T            绵羊白介素6(IL-6)ELISA Kit  Sheep Interleukin 6,IL-6 ELISA Kit  CSB-E10116Sh  96T            大鼠白介素6(IL-6)ELISA KIT  Rat Interleukin 6,IL-6 ELISA KIT  CSB-E04640r  96T            小鼠白介素6(IL-6)ELISA Kit  Mouse Interleukin 6,IL-6 ELISA KIT  CSB-E04639m  96T            猫白介素6(IL-6)ELISA Kit  Cat Interleukin 6(IL-6) ELISA Kit  CSB-E14046Fe  96T            鸡白介素6(IL-6)ELISA Kit  Chicken Interleukin 6,IL-6 ELISA Kit  CSB-E08549Ch  96T            马白介素6(IL-6)ELISA Kit  Horse Interleukin 6(IL-6)ELISA Kit  CSB-E16634Hs  96T            兔子白介素6(IL-6)ELISA Kit  rabbit Interleukin 6,IL-6 ELISA Kit  CSB-E06903Rb  96T            猴白介素6(IL-6)ELISA Kit  Monkey Interleukin 6,IL-6 ELISA Kit  CSB-E10050Mo  96T            山羊白介素6(IL-6)ELISA Kit  Goat Interleukin 6,IL-6 ELISA Kit  CSB-E14360G  96T            犬白介素6(IL-6)ELISA Kit  Dog Interleukin 6,IL-6 ELISA Kit  CSB-E11260c  96T           蛋白  产品名称 货号 规格 蛋白来源  Recombinant Marmota monax Interleukin-6(IL6)  1mg            Recombinant Sparus aurata Interleukin-6(il6)  1mg            Recombinant Mustela putorius furo Interleukin-6(IL6)  1mg            Recombinant Cercocebus atys Interleukin-6(IL6)  1mg            Recombinant Mustela vison Interleukin-6(IL6)  1mg            Recombinant Rat Interleukin-6(Il6)  1mg            Recombinant Macaca fascicularis Interleukin-6(IL6)  1mg            Recombinant Mouse Interleukin-6(Il6)  1mg            Recombinant Human Interleukin-6(IL6)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6)  1mg            Recombinant Cat Interleukin-6(IL6)  1mg            Recombinant Sheep Interleukin-6(IL6)  1mg            Recombinant Pig Interleukin-6(IL6)  1mg            Recombinant Dog Interleukin-6(IL6)  1mg            Recombinant Bovine Interleukin-6(IL6)  1mg

 产品名称： 小鼠白介素6(IL-6)ELISA Kit    英文名称： Mouse Interleukin 6,IL-6 ELISA KIT    别名： BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2    货号： CSB-E04639m    价格： 3200    规格： 96T    种属： Mouse    待测物名称： interleukin 6 (interferon, beta 2)    缩写： IL6    样本类型： serum, plasma, cell culture supernates    检测范围： 1.56 pg/ml-100 pg/ml    灵敏度： 0.39 pg/ml    反应时间： 1-5h    所需样本体积： 50-100ul    检测波长： 450 nm    用途： For research use only. Not for diagnostic use.    原理 ： This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.   特异性： This assay has high sensitivity and excellent specificity for detection of Mouse IL6. No significant cross-reactivity or interference between Mouse IL6 and analogues was observed.   精密度： Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.    样本搜集及储存： Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.    检测步骤： Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.    结果计算： Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.   相关产品：   产品名称 英文名称 货号 规格  猪白介素6(IL-6)ELISA Kit  Pig Interleukin 6,IL-6 ELISA Kit  CSB-E06786p  96T            人白介素6(IL-6)ELISA KIT  Human Interleukin 6,IL-6 ELISA KIT  CSB-E04638h  96T            牛白介素6(IL-6)ELISA Kit  Bovine Interleukin 6,IL-6 ELISA Kit  CSB-E12899B  96T            人超敏白介素6(hs-IL6) ELISA kit  Human high sensitivity interleukin 6 (hs-IL6) ELISA kit  CSB-EQ011664HU  96T            绵羊白介素6(IL-6)ELISA Kit  Sheep Interleukin 6,IL-6 ELISA Kit  CSB-E10116Sh  96T            大鼠白介素6(IL-6)ELISA KIT  Rat Interleukin 6,IL-6 ELISA KIT  CSB-E04640r  96T            猫白介素6(IL-6)ELISA Kit  Cat Interleukin 6(IL-6) ELISA Kit  CSB-E14046Fe  96T            鸡白介素6(IL-6)ELISA Kit  Chicken Interleukin 6,IL-6 ELISA Kit  CSB-E08549Ch  96T            马白介素6(IL-6)ELISA Kit  Horse Interleukin 6(IL-6)ELISA Kit  CSB-E16634Hs  96T            兔子白介素6(IL-6)ELISA Kit  rabbit Interleukin 6,IL-6 ELISA Kit  CSB-E06903Rb  96T            猴白介素6(IL-6)ELISA Kit  Monkey Interleukin 6,IL-6 ELISA Kit  CSB-E10050Mo  96T            山羊白介素6(IL-6)ELISA Kit  Goat Interleukin 6,IL-6 ELISA Kit  CSB-E14360G  96T            犬白介素6(IL-6)ELISA Kit  Dog Interleukin 6,IL-6 ELISA Kit  CSB-E11260c  96T           蛋白  产品名称 货号 规格 蛋白来源  Recombinant Marmota monax Interleukin-6(IL6)  1mg            Recombinant Sparus aurata Interleukin-6(il6)  1mg            Recombinant Mustela putorius furo Interleukin-6(IL6)  1mg            Recombinant Cercocebus atys Interleukin-6(IL6)  1mg            Recombinant Mustela vison Interleukin-6(IL6)  1mg            Recombinant Rat Interleukin-6(Il6)  1mg            Recombinant Macaca fascicularis Interleukin-6(IL6)  1mg            Recombinant Mouse Interleukin-6(Il6)  1mg            Recombinant Human Interleukin-6(IL6)  1mg            Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6)  1mg            Recombinant Cat Interleukin-6(IL6)  1mg            Recombinant Sheep Interleukin-6(IL6)  1mg            Recombinant Pig Interleukin-6(IL6)  1mg            Recombinant Dog Interleukin-6(IL6)  1mg            Recombinant Bovine Interleukin-6(IL6)  1mg

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