产品名称:犬胰岛素(INS)ELISA Kit 英文名称:Dog Insulin,INS ELISA Kit 产品类型: ELISA Kit 货号: CSB-E11327c 价格: 3800 规格: 96T 种属: Dog 待测物名称: insulin 缩写: INS 样本类型: serum, plasma, tissue homogenates 检测范围: 0.625 灵敏度: 0.156 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 产品名称 货号 规格 Human Free Thyroxine,FT4 ELISA Kit CSB-E05078h 96T Chicken Insulin ELISA kit CSB-E13293C 96T Dog Insulin,INS ELISA Kit CSB-E11327c 96T rabbit Insulin,INS ELISA Kit CSB-E06992Rb 96T Rat Free Thyroxine,FT4 ELISA Kit CSB-E05079r 96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05077m 96T Pig Insulin,INS ELISA Kit CSB-E06828p 96T Guinea pig insulin (INS) ELISA kit CSB-EL011742GU 96T Fish Insulin,INS ELISA Kit CSB-E12123Fh 96T Human Insulin,INS ELISA Kit CSB-E05069h 96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05076r 96T Bovine Insulin ELISA kit CSB-E11993B 96T Rat Insulin,INS ELISA Kit CSB-E05070r 96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit CSB-E05074m 96T Sheep Insulin(INS) ELISA Kit CSB-E17044Sh 96T Monkey Insulin,INS ELISA Kit CSB-E10047Mo 96T Horse insulin (INS) ELISA kit CSB-EL011742HO 96T Goat Insulin(INS)ELISA Kit CSB-E17653G 96T Cat insulin (INS) ELISA kit CSB-EL011742CA 96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05075h 96T
新品
2013.10.10
产品名称:猴胰岛素(INS)ELISA Kit 英文名称:Monkey Insulin,INS ELISA Kit 产品类型: ELISA Kit 货号: CSB-E10047Mo 价格: 3800 规格: 96T 种属: Monkey 待测物名称: Insulin,INS 缩写: INS 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 产品名称 货号 规格 Human Free Thyroxine,FT4 ELISA Kit CSB-E05078h 96T Chicken Insulin ELISA kit CSB-E13293C 96T Dog Insulin,INS ELISA Kit CSB-E11327c 96T rabbit Insulin,INS ELISA Kit CSB-E06992Rb 96T Rat Free Thyroxine,FT4 ELISA Kit CSB-E05079r 96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05077m 96T Pig Insulin,INS ELISA Kit CSB-E06828p 96T Guinea pig insulin (INS) ELISA kit CSB-EL011742GU 96T Fish Insulin,INS ELISA Kit CSB-E12123Fh 96T Human Insulin,INS ELISA Kit CSB-E05069h 96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05076r 96T Bovine Insulin ELISA kit CSB-E11993B 96T Rat Insulin,INS ELISA Kit CSB-E05070r 96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit CSB-E05074m 96T Sheep Insulin(INS) ELISA Kit CSB-E17044Sh 96T Monkey Insulin,INS ELISA Kit CSB-E10047Mo 96T Horse insulin (INS) ELISA kit CSB-EL011742HO 96T Goat Insulin(INS)ELISA Kit CSB-E17653G 96T Cat insulin (INS) ELISA kit CSB-EL011742CA 96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05075h 96T
新品
2013.10.10
产品名称:猪胰岛素(INS)ELISA Kit 英文名称:Pig Insulin,INS ELISA Kit 产品类型: ELISA Kit 货号: CSB-E06828p 价格: 3800 规格: 96T 种属: Pig 待测物名称: insulin 缩写: INS 样本类型: serum, plasma, tissue homogenates 检测范围: 2 μIU/ml-400 μIU/ml 灵敏度: 1 μIU/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 产品名称 货号 规格 Human Free Thyroxine,FT4 ELISA Kit CSB-E05078h 96T Chicken Insulin ELISA kit CSB-E13293C 96T Dog Insulin,INS ELISA Kit CSB-E11327c 96T rabbit Insulin,INS ELISA Kit CSB-E06992Rb 96T Rat Free Thyroxine,FT4 ELISA Kit CSB-E05079r 96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05077m 96T Pig Insulin,INS ELISA Kit CSB-E06828p 96T Guinea pig insulin (INS) ELISA kit CSB-EL011742GU 96T Fish Insulin,INS ELISA Kit CSB-E12123Fh 96T Human Insulin,INS ELISA Kit CSB-E05069h 96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05076r 96T Bovine Insulin ELISA kit CSB-E11993B 96T Rat Insulin,INS ELISA Kit CSB-E05070r 96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit CSB-E05074m 96T Sheep Insulin(INS) ELISA Kit CSB-E17044Sh 96T Monkey Insulin,INS ELISA Kit CSB-E10047Mo 96T Horse insulin (INS) ELISA kit CSB-EL011742HO 96T Goat Insulin(INS)ELISA Kit CSB-E17653G 96T Cat insulin (INS) ELISA kit CSB-EL011742CA 96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05075h 96T
新品
2013.10.10
产品名称:小鼠胰岛素(INS)ELISA Kit 英文名称:Mouse Insulin,INS ELISA Kit 产品类型: ELISA Kit 货号: CSB-E05071m 价格: 3200 规格: 96T 种属: Mouse 待测物名称: Insulin,INS 缩写: INS 样本类型: serum, plasma, cell culture supernates, tissue homogenates, cell lysates 检测范围: 15.6 nIU/ml -1000 nIU/ml 灵敏度: 3.9 nIU/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 产品名称 货号 规格 Human Free Thyroxine,FT4 ELISA Kit CSB-E05078h 96T Chicken Insulin ELISA kit CSB-E13293C 96T Dog Insulin,INS ELISA Kit CSB-E11327c 96T rabbit Insulin,INS ELISA Kit CSB-E06992Rb 96T Rat Free Thyroxine,FT4 ELISA Kit CSB-E05079r 96T Mouse Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05077m 96T Pig Insulin,INS ELISA Kit CSB-E06828p 96T Guinea pig insulin (INS) ELISA kit CSB-EL011742GU 96T Fish Insulin,INS ELISA Kit CSB-E12123Fh 96T Human Insulin,INS ELISA Kit CSB-E05069h 96T Rat Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05076r 96T Bovine Insulin ELISA kit CSB-E11993B 96T Rat Insulin,INS ELISA Kit CSB-E05070r 96T Mouse Neonatal Thyroxine,NN-T4 ELISA Kit CSB-E05074m 96T Sheep Insulin(INS) ELISA Kit CSB-E17044Sh 96T Monkey Insulin,INS ELISA Kit CSB-E10047Mo 96T Horse insulin (INS) ELISA kit CSB-EL011742HO 96T Goat Insulin(INS)ELISA Kit CSB-E17653G 96T Cat insulin (INS) ELISA kit CSB-EL011742CA 96T Human Free Tri-iodothyronine Indes,Free-T3 ELISA Kit CSB-E05075h 96T
新品
2013.10.10
产品名称:兔子胰岛素(INS)ELISA Kit 英文名称:rabbit Insulin,INS ELISA Kit 产品类型: ELISA Kit 货号: CSB-E06992Rb 价格: 3800 规格: 96T 种属: Rabbit 待测物名称: insulin 缩写: INS 样本类型: serum, plasma, tissue homogenates 检测范围: 8 μIU/ml-140 μIU/ml 灵敏度: 5 μIU/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 产品名称 货号 规格 Chicken Insulin ELISA kit CSB-E13293C 96T Dog Insulin,INS ELISA Kit CSB-E11327c 96T Pig Insulin,INS ELISA Kit CSB-E06828p 96T Guinea pig insulin (INS) ELISA kit CSB-EL011742GU 96T Fish Insulin,INS ELISA Kit CSB-E12123Fh 96T Mouse Insulin,INS ELISA Kit CSB-E05071m 96T Human Insulin,INS ELISA Kit CSB-E05069h 96T Bovine Insulin ELISA kit CSB-E11993B 96T Rat Insulin,INS ELISA Kit CSB-E05070r 96T Sheep Insulin(INS) ELISA Kit CSB-E17044Sh 96T Monkey Insulin,INS ELISA Kit CSB-E10047Mo 96T Horse insulin (INS) ELISA kit CSB-EL011742HO 96T Goat Insulin(INS)ELISA Kit CSB-E17653G 96T Cat insulin (INS) ELISA kit CSB-EL011742CA 96T 抗体 产品名称 货号 规格 Rabbit anti-human Insulin(INS) polyclonal Antibody CSB-PA011742GA01HU 150ul 蛋白 产品名称 规格 Recombinant Verasper moseri Insulin(ins) 1mg Recombinant Ornithorhynchus anatinus Insulin(INS) 1mg Recombinant Aplysia californica Insulin(PIN) 1mg Recombinant Pantodon buchholzi Insulin(ins) 1mg Recombinant Spermophilus tridecemlineatus Insulin(INS) 1mg
新品
2013.10.10
产品名称:人胰岛素(INS)ELISA Kit 英文名称:Human Insulin,INS ELISA Kit 产品类型: ELISA Kit 货号: CSB-E05069h 价格: 3600 规格: 96T 种属: Human 待测物名称: insulin 缩写: INS 样本类型: serum, plasma, cell culture supernates, tissue homogenates 检测范围: 8μIU/ml-140μIU/ml 灵敏度: 5μIU/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IN......+This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INS bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Human INS. No significant cross-reactivity or interference between Human INS and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......+Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......+Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the INS concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称 货号 规格 Chicken Insulin ELISA kit CSB-E13293C 96T Dog Insulin,INS ELISA Kit CSB-E11327c 96T rabbit Insulin,INS ELISA Kit CSB-E06992Rb 96T Pig Insulin,INS ELISA Kit CSB-E06828p 96T Guinea pig insulin (INS) ELISA kit CSB-EL011742GU 96T Fish Insulin,INS ELISA Kit CSB-E12123Fh 96T Mouse Insulin,INS ELISA Kit CSB-E05071m 96T Bovine Insulin ELISA kit CSB-E11993B 96T Rat Insulin,INS ELISA Kit CSB-E05070r 96T Sheep Insulin(INS) ELISA Kit CSB-E17044Sh 96T Monkey Insulin,INS ELISA Kit CSB-E10047Mo 96T Horse insulin (INS) ELISA kit CSB-EL011742HO 96T Goat Insulin(INS)ELISA Kit CSB-E17653G 96T Cat insulin (INS) ELISA kit CSB-EL011742CA 96T 抗体 产品名称 货号 规格 Rabbit anti-human Insulin(INS) polyclonal Antibody CSB-PA011742GA01HU 150ul 蛋白 产品名称 规格 Recombinant Verasper moseri Insulin(ins) 1mg Recombinant Ornithorhynchus anatinus Insulin(INS) 1mg Recombinant Aplysia californica Insulin(PIN) 1mg Recombinant Pantodon buchholzi Insulin(ins) 1mg Recombinant Spermophilus tridecemlineatus Insulin(INS) 1mg
新品
2013.10.10
产品名称:大鼠胰岛素(INS)ELISA Kit 英文名称:Rat Insulin,INS ELISA Kit 产品类型: ELISA Kit 货号: CSB-E05070r 价格: 3800 规格: 96T 种属: Rat 待测物名称: Insulin,INS 缩写: INS 样本类型: serum, plasma, cell culture supernates 检测范围: 15.6 nIU/ml-1000 nIU/ml 灵敏度: 3.9 nIU/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 产品名称 货号 规格 Human Free Thyroxine,FT4 ELISA Kit CSB-E05078h 96T Chicken Insulin ELISA kit CSB-E13293C 96T Dog Insulin,INS ELISA Kit CSB-E11327c 96T rabbit Insulin,INS ELISA Kit CSB-E06992Rb 96T Rat Free Thyroxine,FT4 ELISA Kit CSB-E05079r 96T
新品
2013.10.10
CUSABIO 2013开学巨献 活动一 开学季,CUSABIO好礼送不停:凡订购任意2个CUSABIO试剂盒(96T),或购买指定类别任意产品满3500元即可获赠精美礼品一份 * 8G U盘 * 价值50元移动充值卡 * 价值50元DQ冰雪皇后冰淇淋缤纷卡 以上礼品,任选其一,多买多送! 参与活动产品类别: 试剂盒 天然蛋白 多克隆抗体 单克隆抗体 RT-PCR引物对 活动二 开学季, CUSABIO好礼送不停之克隆,重组蛋白产品惊喜促销! 活动期间,购买任意一款克隆,重组蛋白产品,即送30元移动充值卡一张,多买多送! 参与活动产品类别: 克隆 重组蛋白 礼品多多,机会属于你!! 活动时间:2013年9月1日至2013年10月31日 本活动解释权---上海飞轩生物科技有限公司
厂商
2013.09.06
产品名称:牛肾上腺髓质素(ADM)ELISA kit 英文名称:Bovine adrenomedullin,ADM ELISA kit 产品类型: ELISA Kit 货号: CSB-EL001370BO 价格: 4200 规格: 96T 种属: Bovine 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......+This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.―特异性: This assay has high sensitivity and excellent specificity for detection of Bovine ADM. No significant cross-reactivity or interference between Bovine ADM and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......+Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......+Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称 货号 规格 Human adrenomedullin,ADM ELISA Kit CSB-E09146h 96T Dog adrenomedullin,ADM ELISA Kit CSB-E09836c 96T Fish adrenomedullin (ADM) ELISA kit CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit CSB-E10061m 96T Rat adrenomedullin,ADM ELISA Kit CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit CSB-E09837Rb 96T Pig adrenomedullin (ADM) ELISA kit CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit CSB-E04843Ch 96T 蛋白 产品名称 规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg
新品
2013.09.04
产品名称:犬肾上腺髓质素(ADM)ELISA Kit 英文名称:Dog adrenomedullin,ADM ELISA Kit 产品类型: ELISA Kit 货号: CSB-E09836c 价格: 4200 规格: 96T 种属: Dog 样本类型: serum, plasma, tissue homogenates, cell culture supernates 检测范围: 15.6 pg/ml-1000 pg/ml 灵敏度: 3.9 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......+This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Dog ADM. No significant cross-reactivity or interference between Dog ADM and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......+Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......+Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称 货号 规格 Human adrenomedullin,ADM ELISA Kit CSB-E09146h 96T Bovine adrenomedullin (ADM) ELISA kit CSB-EL001370BO 96T Fish adrenomedullin (ADM) ELISA kit CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit CSB-E10061m 96T Rat adrenomedullin,ADM ELISA Kit CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit CSB-E09837Rb 96T Pig adrenomedullin (ADM) ELISA kit CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit CSB-E04843Ch 96T 蛋白 产品名称 规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg
新品
2013.09.04
产品名称:大鼠肾上腺髓质素(ADM)ELISA Kit 英文名称:Rat adrenomedullin,ADM ELISA Kit 产品类型: ELISA Kit 货号: CSB-E10060r 价格: 3900 规格: 96T 种属: Rat 检测范围: 0.312 nmol/ml-20 nmol/ml 灵敏度: 0.078 nmol/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......+This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Rat ADM. No significant cross-reactivity or interference between Rat ADM and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......+Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......+Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称 货号 规格 Dog adrenomedullin,ADM ELISA Kit CSB-E09836c 96T Human adrenomedullin,ADM ELISA Kit CSB-E09146h 96T Bovine adrenomedullin,ADM ELISA kit CSB-EL001370BO 96T Fish adrenomedullin,ADM ELISA kit CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit CSB-E10061m 96T Rabbit adrenomedullin,ADM ELISA Kit CSB-E09837Rb 96T Pig adrenomedullin,ADM ELISA Kit CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit CSB-E04843Ch 96T 蛋白 产品名称 规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg
新品
2013.09.04
产品名称:人肾上腺髓质素(ADM)ELISA Kit 英文名称:Human adrenomedullin,ADM ELISA Kit 产品类型: ELISA Kit 货号: CSB-E09146h 价格: 3600 规格: 96T 种属: Human 样本类型: serum, plasma, tissue homogenates 检测范围: 0.312 pg/ml-20 pg/ml 灵敏度: 0.078 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450nm 用途: For research use only. Not for diagnostic use. 原理: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......+This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.―特异性: This assay has high sensitivity and excellent specificity for detection of Human ADM. No significant cross-reactivity or interference between Human ADM and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......+Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......+Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称 货号 规格 Dog adrenomedullin,ADM ELISA Kit CSB-E09836c 96T Bovine adrenomedullin (ADM) ELISA kit CSB-EL001370BO 96T Fish adrenomedullin (ADM) ELISA kit CSB-EL001370FI 96T Mouse adrenomedullin,ADM ELISA Kit CSB-E10061m 96T Rat adrenomedullin,ADM ELISA Kit CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit CSB-E09837Rb 96T Pig adrenomedullin (ADM)ELISA kit CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit CSB-E04843Ch 96T 蛋白 产品名称 规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg
新品
2013.09.04
产品名称:小鼠肾上腺髓质素(ADM)ELISA Kit 英文名称:Mouse adrenomedullin,ADM ELISA Kit 产品类型: ELISA Kit 货号: CSB-E10061m 价格: 3800 规格: 96T 种属: Mouse 检测范围: 4.7 pg/ml-300 pg/ml 灵敏度: 1.2 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 标准曲线: 用途: For research use only. Not for diagnostic use. 原理: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AD......+This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Mouse ADM. No significant cross-reactivity or interference between Mouse ADM and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediat......+Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1......+Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract......+Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the ADM concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称 货号 规格 Dog adrenomedullin,ADM ELISA Kit CSB-E09836c 96T Human adrenomedullin,ADM ELISA Kit CSB-E09146h 96T Bovine adrenomedullin (ADM) ELISA kit CSB-EL001370BO 96T Fish adrenomedullin (ADM) ELISA kit CSB-EL001370FI 96T Rat adrenomedullin,ADM ELISA Kit CSB-E10060r 96T Rabbit adrenomedullin,ADM ELISA Kit CSB-E09837Rb 96T Pig adrenomedullin (ADM) ELISA kit CSB-EL001370PI 96T Monkey adrenomedullin,ADM ELISA Kit CSB-E09838Mo 96T Chicken adrenomedullin,ADM ELISA Kit CSB-E04843Ch 96T 蛋白 产品名称 规格 Recombinant Bovine ADM(ADM) 1mg Recombinant Dog ADM(ADM) 1mg Recombinant Pig ADM(ADM) 1mg Recombinant Human ADM(ADM) 1mg Recombinant Rat ADM(Adm) 1mg Recombinant Mouse ADM(Adm) 1mg
新品
2013.09.04
产品名称: 人肌酸激酶同工酶BB(CK-BB)ELISA Kit 英文名称: Human creatine kinase BB isoenzyme,CK-BB ELISA kit 货号: CSB-EQ027522HU 价格: 3600 规格: 96T 种属: Human 待测物名称: creatine kinase BB isoenzyme (CK-BB) 缩写: CK-BB 样本类型: serum, plasma, tissue homogenates 检测范围: 3.12 mU/ml-200 mU/ml 灵敏度: 0.78mU/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450nm 用途: For research use only. Not for diagnostic use. 相关产品: 产品名称 英文名称 货号 规格 兔肌酸激酶同工酶BB(CK-BB)ELISA Kit Rabbit creatine kinase BB isoenzyme (CK-BB) ELISA kit CSB-EQ027522RB 96T 小鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit Mouse creatine kinase BB isoenzyme (CK-BB) ELISA kit CSB-EQ027522MO 96T 大鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit Rat creatine kinase BB isoenzyme (CK-BB) ELISA kit CSB-EQ027522RA 96T 牛肌酸激酶同工酶BB(CK-BB)ELISA Kit Bovine Creatine Kinase BB Isoenzymes,CK-BB ELISA Kit CSB-E14138B 96T
新品
2013.05.18
产品名称: 牛肌酸激酶同工酶BB(CK-BB)ELISA Kit 英文名称: Bovine Creatine Kinase BB Isoenzymes,CK-BB ELISA Kit 货号: CSB-E14138B 规格: 96T 种属: Bovine 待测物名称: Creatine Kinase BB Isoenzymes,CK-BB 缩写: CK-BB 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 相关产品: 产品名称 英文名称 货号 规格 大鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit Rat creatine kinase BB isoenzyme (CK-BB) ELISA kit CSB-EQ027522RA 96T 人肌酸激酶同工酶BB(CK-BB)ELISA Kit Human creatine kinase BB isoenzyme (CK-BB) ELISA kit CSB-EQ027522HU 96T 牛凝血酶抗凝血酶Ⅲ复合物(TAT-III)ELISA Kit Bovine Thrombin-antithrombin III Complex, TAT-III ELISA Kit CSB-E14133B 96T 小鼠幽门螺旋杆菌抗体(IgM)ELISA Kit Mouse Helicobacter pylori antibody(IgM)ELISA Kit CSB-E14131m 96T 小鼠幽门螺旋杆菌抗体(IgG)ELISA Kit Mouse Helicobacter pylori antibody(IgG)ELISA Kit CSB-E14132m 96T 牛内皮型一氧化氮合成酶(eNOS)ELISA Kit Bovine Endothelial nitric oxide synthase,eNOS ELISA Kit CSB-E14137B 96T 牛诱导型一氧化氮合成酶(iNOS)ELISA Kit Bovine Inducible Nitric Oxide Synthase,iNOS ELISA Kit CSB-E14135B 96T 牛神经型一氧化氮合成酶(nNOS/NOS1)ELISA Kit Bovine Neuronal Nitric Oxide Synthase, nNOS/NOS1 ELISA Kit CSB-E14136B 96T 牛肌酸激酶同工酶MB(CK-MB)ELISA Kit Bovine Creatine Kinase MB Isoenzyme,CK-MB ELISA Kit CSB-E14139B 96T 大鼠激动异构酶/细胞分裂素MB(CKMB)ELISA Kit Rat cytokinin MB,CKMB ELISA Kit CSB-E14130r 96T
新品
2013.05.18
产品名称: 大鼠肌酸激酶同工酶BB(CK-BB)ELISA Kit 英文名称: Rat creatine kinase BB isoenzyme,CK-BB ELISA kit 货号: CSB-EQ027522RA 规格: 96T 种属: Rat 待测物名称: creatine kinase BB isoenzyme (CK-BB) 缩写: CK-BB 样本类型: serum, plasma, tissue homogenates 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450nm 用途: For research use only. Not for diagnostic use. 相关产品: 产品名称 英文名称 货号 规格 人肌酸激酶同工酶BB(CK-BB)ELISA Kit Human creatine kinase BB isoenzyme (CK-BB) ELISA kit CSB-EQ027522HU 96T 牛肌酸激酶同工酶BB(CK-BB)ELISA Kit Bovine Creatine Kinase BB Isoenzymes,CK-BB ELISA Kit CSB-E14138B 96T
新品
2013.05.18
产品名称: 小鼠肾损伤分子1(Kim-1)ELISA Kit 英文名称: Mouse Kidney injury molecule 1,Kim-1 ELISA Kit 别名: HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1 货号: CSB-E08809m 规格: 96T 种属: Mouse 待测物名称: hepatitis A virus cellular receptor 1 缩写: HAVCR1 检测范围: 0.94 ng/ml-60 ng/ml 灵敏度: 0.23 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Mouse HAVCR1. No significant cross-reactivity or interference between Mouse HAVCR1 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 大鼠肾损伤分子1(Kim-1)ELISA Kit Rat Kidney injury molecule 1,Kim-1 ELISA Kit CSB-E08808r 96T 人肾损伤分子1(Kim-1)ELISA Kit Human Kidney injury molecule 1,Kim-1 ELISA Kit CSB-E08807h 96T 猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit CSB-EL010144PI 96T 猴肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit CSB-EL010144RH 96T 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) 1mg
新品
2013.05.15
产品名称: 大鼠肾损伤分子1(Kim-1)ELISA Kit 英文名称: Rat Kidney injury molecule 1,Kim-1 ELISA Kit 别名: HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1 货号: CSB-E08808r 规格: 96T 种属: Rat 待测物名称: hepatitis A virus cellular receptor 1 缩写: HAVCR1 样本类型: serum, plasma, urine 检测范围: 0.312 ng/ml-20 ng/ml 灵敏度: 0.078 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Rat HAVCR1. No significant cross-reactivity or interference between Rat HAVCR1 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 产品名称 英文名称 货号 规格 人肾损伤分子1(Kim-1)ELISA Kit Human Kidney injury molecule 1,Kim-1 ELISA Kit CSB-E08807h 96T 猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit CSB-EL010144PI 96T 小鼠肾损伤分子1(Kim-1)ELISA Kit Mouse Kidney injury molecule 1,Kim-1 ELISA Kit CSB-E08809m 96T 猴肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit CSB-EL010144RH 96T 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Human Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) 1mg Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) 1mg
新品
2013.05.15
产品名称: 人肾损伤分子1(Kim-1)ELISA Kit 英文名称: Human Kidney injury molecule 1,Kim-1 ELISA Kit 别名: HAVCR, HAVCR-1, KIM-1, KIM1, TIM-1, TIM1, TIMD1 货号: CSB-E08807h 规格: 96T 种属: Human 待测物名称: hepatitis A virus cellular receptor 1 缩写: HAVCR1 蛋白功能1: Immunity 蛋白功能3: Host-virus interaction 样本类型: serum, urine, tissue homogenates 检测范围: 0.312 ng/ml-20 ng/ml 灵敏度: 0.078 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAVCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAVCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAVCR1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAVCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Human HAVCR1. No significant cross-reactivity or interference between Human HAVCR1 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HAVCR1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 大鼠肾损伤分子1(Kim-1)ELISA Kit Rat Kidney injury molecule 1,Kim-1 ELISA Kit CSB-E08808r 96T 猪肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit Pig hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit CSB-EL010144PI 96T 小鼠肾损伤分子1(Kim-1)ELISA Kit Mouse Kidney injury molecule 1,Kim-1 ELISA Kit CSB-E08809m 96T 猴肝炎病毒细胞的受体1/肾损伤分子1/KIM1(HAVCR1) ELISA kit Monkey hepatitis A virus cellular receptor 1/KIM1(HAVCR1) ELISA kit CSB-EL010144RH 96T 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) CSB-EP010144DSU 1mg E.coli Recombinant Chlorocebus aethiops Hepatitis A virus cellular receptor 1(HAVCR1) CSB-YP010144DSU 1mg Yeast Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) CSB-EP010144RA 1mg E.coli Recombinant Rat Hepatitis A virus cellular receptor 1 homolog(Havcr1) CSB-YP010144RA 1mg Yeast
新品
2013.05.15
产品名称: 犬肾损伤分子1(Kim-1)ELISA Kit 英文名称: Canine kidney injury molecule 1,Kim-1 ELISA Kit 货号: CSB-E13035c 规格: 96T 种属: Dog 待测物名称: kidney injury molecule 1(Kim-1) 缩写: Kim-1 样本类型: serum, plasma, tissue homogenates 检测范围: 0.312 ng/ml-20 ng/ml 灵敏度: 0.078ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 相关产品: 产品名称 英文名称 货号 规格 大鼠不对称二甲基精氨酸(ADMA)ELISA Kit Rat asymmetrical dimethylarginine(ADMA) ELISA Kit CSB-E13039r 96T 大鼠一氧化氮(NO)ELISA Kit Rat nitric oxide (NO) ELISA Kit CSB-E13034r 96T 大鼠肿瘤坏死因子γ(TNF-γ)ELISA Kit Rat tumor necrosis factor γ (TNF-γ) ELISA Kit CSB-E13033r 96T 鸡白介素6受体(IL-6R)ELISA Kit Chicken Interleukin 6 receptor (IL-6R) ELISA Kit CSB-E13030C 96T 豚鼠白介素18(IL-18)ELISA Kit Guinea pig Interleukin 18(IL-18) ELISA Kit CSB-E13032Gu 96T 鸡白介素15(IL-15)ELISA Kit Chicken Interleukin 15(IL-15) ELISA Kit CSB-E13031C 96T 大鼠血红蛋白ELISA Kit Rat Hemoglobin (Hb) ELISA Kit CSB-E13036r 96T 人CD82分子ELISA Kit Human cluster Of differentiation 82 (CD82) ELISA Kit CSB-E13037h 96T
新品
2013.05.15
产品名称: 小鼠白介素1β (IL-1β)ELISA Kit 英文名称: Mouse Interleukin 1β,IL-1β ELISA Kit 别名: IL-1, IL1-BETA, IL1F2 货号: CSB-E08054m 价格: 3200 规格: 96T 种属: Mouse 待测物名称: interleukin 1, beta 缩写: IL1B 样本类型: serum, plasma, cell culture supernates, tissue homogenates 检测范围: 31.25 pg/ml-2000 pg/ml 灵敏度: 7.81 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1B bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Mouse IL1B. No significant cross-reactivity or interference between Mouse IL1B and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL1B concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 绵羊白介素1β(IL-1β)ELISA Kit Sheep Interleukin 1β,IL-1β ELISA Kit CSB-E10115Sh 96T 大鼠白介素1β前体(pro-IL1B)ELISA Kit Rat pro interleukin 1 beta (pro-IL1B) ELISA Kit CSB-ET011614RA 96T 犬白介素1β(IL-1β) ELISA Kit Dog Interleukin 1β (IL- 1β) ELISA Kit CSB-E13836c 96T 兔子白介素1β (IL-1β)ELISA Kit Rabbit Interleukin 1β,IL-1β ELISA Kit CSB-E06900Rb 96T 牛白介素1β(IL-1β) ELISA kit Bovine Interleukin 1β (IL- 1β) ELISA kit CSB-E12986B 96T 豚鼠白介素1β(IL1B) ELISA kit Guinea pig Interleukin-1 beta(IL1B) ELISA kit CSB-EL011614GU 96T 山羊白介素1β(IL-1β)ELISA Kit Goat Interleukin 1β(IL-1β) ELISA Kit CSB-E16568G 96T 人白介素1β (IL-1β)ELISA Kit Human Interleukin 1β,IL-1β ELISA Kit CSB-E08053h 96T 马白介素1β(IL-1β)ELISA Kit Horse Interleukin 1β(IL-1β)ELISA Kit CSB-E16635Hs 96T 人白介素1β前体(pro-IL1B)ELISA Kit Human pro interleukin 1 beta (pro-IL1B) ELISA Kit CSB-ET011614HU 96T 大鼠白介素1β (IL-1β)ELISA Kit Rat Interleukin 1β,IL-1β ELISA Kit CSB-E08055r 96T 小鼠白介素1β前体(pro-IL1B)ELISA Kit Mouse pro interleukin 1 beta (pro-IL1B) ELISA Kit CSB-ET011614MO 96T 猪白介素1β (IL-1β)ELISA Kit Pig Interleukin 1β,IL-1β ELISA Kit CSB-E06782p 96T Monkey Interleukin-1 beta(IL1B) ELISA kit Monkey Interleukin-1 beta(IL1B) ELISA kit CSB-EL011614RH 96T Cat Interleukin-1 beta(IL1B) ELISA kit Cat Interleukin-1 beta(IL1B) ELISA kit CSB-EL011614CA 96T 抗体 产品名称 货号 规格 Rabbit anti-human interleukin 1, beta polyclonal Antibody CSB-PA011614GA01HU 150ul 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Mustela putorius furo Interleukin-1 beta(IL1B) 1mg Recombinant Cercocebus atys Interleukin-1 beta(IL1B) 1mg Recombinant Cervus elaphus Interleukin-1 beta(IL1B) 1mg Recombinant Macaca nemestrina Interleukin-1 beta(IL1B) 1mg Recombinant Macaca fascicularis Interleukin-1 beta(IL1B) 1mg Recombinant Human Interleukin-1 beta(IL1B) 1mg Recombinant Bovine Interleukin-1 beta(IL1B) 1mg Recombinant Macaca mulatta (Rhesus macaque) Interleukin-1 beta(IL1B) 1mg Recombinant Cat Interleukin-1 beta(IL1B) 1mg Recombinant Pig Interleukin-1 beta(IL1B) 1mg Recombinant Sheep Interleukin-1 beta(IL1B) 1mg Recombinant Rabbit Interleukin-1 beta(IL1B) 1mg Recombinant Mouse Interleukin-1 beta(Il1b) 1mg Recombinant Goat Interleukin-1 beta(IL1B) 1mg
新品
2013.05.09
产品名称: 小鼠白介素1α(IL-1α)ELISA Kit 英文名称: Mouse Interleukin 1α,IL-1α ELISA kit 别名: IL-1A, IL1, IL1-ALPHA, IL1F1, IL1A (IL1F1) 货号: CSB-E04621m 价格: 3200 规格: 96T 种属: Mouse 待测物名称: interleukin 1, alpha 缩写: IL1A 蛋白功能1: Immunity 蛋白功能3: Inflammatory response 样本类型: serum, plasma, tissue homogenates, cell culture supernates 检测范围: 1.56 pg/ml-100 pg/ml 灵敏度: 0.39 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1A bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Mouse IL1A. No significant cross-reactivity or interference between Mouse IL1A and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL1A concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 Bovine Interleukin-1 alpha(IL1A) ELISA kit Bovine Interleukin-1 alpha(IL1A) ELISA kit CSB-EL011613BO 96T 大鼠白介素1α(IL-1α)ELISA kit Rat Interleukin 1α ,IL-1α ELISA kit CSB-E04622r 96T 兔子白介素1α (IL-1α)ELISA Kit Rabbit Interleukin 1α,IL-1α ELISA Kit CSB-E13154Rb 96T 绵羊白介素1α(IL-1α)ELISA Kit Sheep Interleukin 1α(IL-1α)ELISA Kit CSB-E15096Sh 96T 猪白介素1α(IL-1α)ELISA kit Pig Interleukin 1α ,IL-1α ELISA kit CSB-E09725p 96T 犬白介素1α(IL1A)ELISA kit Dog Interleukin-1 alpha(IL1A) ELISA kit CSB-EL011613DO 96T 人白介素1α(IL-1α )ELISA Kit Human Interleukin 1α,IL-1α ELISA Kit CSB-E04620h 96T 猴白介素1a(IL1A)ELISA Kit Monkey Interleukin-1 alpha (IL1A) ELISA kit CSB-E10041Mo 96T 山羊白介素1α(IL-1α)ELISA Kit Goat Interleukin 1α(IL-1α) ELISA Kit CSB-E15099G 96T Horse Interleukin-1 alpha(IL1A) ELISA kit Horse Interleukin-1 alpha(IL1A) ELISA kit CSB-EL011613HO 96T Guinea pig Interleukin-1 alpha(IL1A) ELISA kit Guinea pig Interleukin-1 alpha(IL1A) ELISA kit CSB-EL011613GU 96T Cat Interleukin-1 alpha(IL1A) ELISA kit Cat Interleukin-1 alpha(IL1A) ELISA kit CSB-EL011613CA 96T 抗体 产品名称 货号 规格 Rabbit anti-human interleukin 1, alpha polyclonal Antibody CSB-PA011613GA01HU 150ul 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Cercocebus atys Interleukin-1 alpha(IL1A) 1mg Recombinant Rat Interleukin-1 alpha(Il1a) 1mg Recombinant Macaca fascicularis Interleukin-1 alpha(IL1A) 1mg Recombinant Dog Interleukin-1 alpha(IL1A) 1mg Recombinant Cat Interleukin-1 alpha(IL1A) 1mg Recombinant Rabbit Interleukin-1 alpha(IL1A) 1mg Recombinant Bovine Interleukin-1 alpha(IL1A) 1mg Recombinant Human Interleukin-1 alpha(IL1A) 1mg Recombinant Mouse Interleukin-1 alpha(Il1a) 1mg Recombinant Macaca mulatta (Rhesus macaque) Interleukin-1 alpha(IL1A) 1mg Recombinant Pig Interleukin-1 alpha(IL1A) 1mg Recombinant Goat Interleukin-1 alpha(IL1A) 1mg
新品
2013.05.09
产品名称: 人转化生长因子β1(TGF-β1)ELISA kit 英文名称: Human Transforming Growth factor β1,TGF-β1 ELISA kit 别名: CED, DPD1, LAP, TGFB, TGFbeta, TGF-beta 1 protein|latency-associated peptide 货号: CSB-E04725h 规格: 96T 种属: Human 待测物名称: transforming growth factor, beta 1 缩写: TGFB1 样本类型: serum, plasma, cell culture supernates 检测范围: 0.8 ng/ml-50 ng/ml 灵敏度: 0.2 ng/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TGFB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TGFB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TGFB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TGFB1 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Human TGFB1. No significant cross-reactivity or interference between Human TGFB1 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the TGFB1 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 绵羊转化生长因子β1(TGFB1)ELISA kit Sheep Transforming growth factor beta-1(TGFB1) ELISA kit CSB-EL023446SH 96T 大鼠转化生长因子β1(TGF-β1)ELISA kit Rat Transforming Growth Factor β1(TGF-β1) ELISA kit CSB-E04727r 96T 鱼转化生长因子β1(TGFB1) ELISA kit Fish transforming growth factor, beta 1 (TGFB1) ELISA kit CSB-EL023446FI 96T 牛转化生长因子β1(TGF-β1)ELISA Kit Bovine transforming growth factor β1,TGF-β1 ELISA Kit CSB-E14208B 96T 猪转化生长因子β1(TGF-β1)ELISA Kit Pig transforming growth factor β1(TGF-β1)ELISA Kit CSB-E06843p 96T 小鼠转化生长因子β1(TGF-β1)ELISA Kit Mouse Transforming Growth factor β1,TGF-β1 ELISA kit CSB-E04726m 96T Horse Transforming growth factor beta-1(TGFB1) ELISA kit Horse Transforming growth factor beta-1(TGFB1) ELISA kit CSB-EL023446HO 96T 豚鼠转化生长因子β1(TGF-β1)ELISA Kit guinea pig transforming growth factor β1,TGF-β1 ELISA Kit CSB-E06773Gu 96T 犬转移生长因子β1(TGF-β1)ELISA Kit Dog Transforming growth factor β1,TGF-β1 ELISA Kit CSB-E04796c 96T 鸡转化生长因子β1(TGF-β1)ELISA kit Chicken Transforming Growth factor β1,TGF-β1 ELISA kit CSB-E09875Ch 96T 抗体 产品名称 货号 规格 Rabbit anti-human transforming growth factor, beta 1 polyclonal Antibody CSB-PA023446GA01HU 150ul 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Oncorhynchus mykiss Transforming growth factor beta-1(tgfb1) CSB-EP023446OEI 1mg E.coli Recombinant Oncorhynchus mykiss Transforming growth factor beta-1(tgfb1) CSB-YP023446OEI 1mg Yeast Recombinant Chlorocebus aethiops Transforming growth factor beta-1(TGFB1) CSB-EP023446DSU 1mg E.coli Recombinant Chlorocebus aethiops Transforming growth factor beta-1(TGFB1) CSB-YP023446DSU 1mg Yeast Recombinant Xenopus laevis Transforming growth factor beta-1(tgfb1) CSB-EP023446XBE 1mg E.coli Recombinant Xenopus laevis Transforming growth factor beta-1(tgfb1) CSB-YP023446XBE 1mg Yeast Recombinant Horse Transforming growth factor beta-1(TGFB1) CSB-EP023446HO 1mg E.coli Recombinant Horse Transforming growth factor beta-1(TGFB1) CSB-YP023446HO 1mg Yeast Recombinant Chicken Transforming growth factor beta-1(TGFB1) CSB-EP023446CH 1mg E.coli Recombinant Chicken Transforming growth factor beta-1(TGFB1) CSB-YP023446CH 1mg Yeast Recombinant Pig Transforming growth factor beta-1(TGFB1) CSB-EP023446PI 1mg E.coli Recombinant Pig Transforming growth factor beta-1(TGFB1) CSB-YP023446PI 1mg Yeast Recombinant Human Transforming growth factor beta-1(TGFB1) CSB-EP023446HU 1mg E.coli Recombinant Human Transforming growth factor beta-1(TGFB1) CSB-YP023446HU 1mg Yeast Recombinant Mouse Transforming growth factor beta-1(Tgfb1) CSB-EP023446MO 1mg E.coli Recombinant Mouse Transforming growth factor beta-1(Tgfb1) CSB-YP023446MO 1mg Yeast Recombinant Dog Transforming growth factor beta-1(TGFB1) CSB-EP023446DO 1mg E.coli Recombinant Dog Transforming growth factor beta-1(TGFB1) CSB-YP023446DO 1mg Yeast Recombinant Sheep Transforming growth factor beta-1(TGFB1) CSB-EP023446SH 1mg E.coli Recombinant Sheep Transforming growth factor beta-1(TGFB1) CSB-YP023446SH 1mg Yeast Recombinant Bovine Transforming growth factor beta-1(TGFB1) CSB-EP023446BO 1mg E.coli Recombinant Bovine Transforming growth factor beta-1(TGFB1) CSB-YP023446BO 1mg Yeast Recombinant Rat Transforming growth factor beta-1(Tgfb1) CSB-EP023446RA 1mg E.coli Recombinant Rat Transforming growth factor beta-1(Tgfb1) CSB-YP023446RA 1mg Yeast
厂商
2013.05.07
产品名称: 猪口蹄疫抗体(IgM)ELISA Kit 英文名称: Pig Antibody against Foot and Mouth Disease(FMDV)IgM ELISA kit 货号: CSB-E12768p 价格: 5000 规格: 192T 种属: Pig 待测物名称: Antibody against Foot and Mouth Disease(FMDV) IgM 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 相关产品: 产品名称 英文名称 货号 规格 猪瘟抗体(IgG) ELISA Kit Classical Swine Fever Virus Antibody(IgG) ELISA Kit CSB-E12765p 192T 犬肾上腺素(EPI)ELISA Kit Canine Epinephrine/Adrenaline,EPI ELISA Kit CSB-E12761c 96T 犬髓过氧化物酶(MPO)ELISA Kit Dog Myeloperoxidase (MPO) ELISA kit CSB-E12762c 96T 猪口蹄疫抗体(IgG) ELISA Kit Pig Foot and Mouth Disease Virus Antibody (IgG) ELISA Kit CSB-E12767p 192T 猪瘟抗体(IgM) ELISA Kit Anti-CSFV Antibody (IgM) ELISA Kit CSB-E12766p 192T 犬促肾上皮质激素释放激素(CRH)ELISA Kit Dog corticotropin releasing hormone,CRH ELISA Kit CSB-E12763c 96T 大鼠P0蛋白 ELISA kit Rat myelin protein zero (p0) ELISA Kit CSB-E12769r 96T 犬皮质酮/肾上腺酮(CORT)ELISA Kit Canine Corticosterone,CORT ELISA Kit CSB-E12760c 96T
商机
2013.04.17
产品名称: 猪口蹄疫抗体(IgG)ELISA Kit 英文名称: Pig Foot and Mouth Disease Virus Antibody(IgG)ELISA Kit 货号: CSB-E12767p 价格: 5000 规格: 192T 种属: Pig 待测物名称: Antibody against Foot and Mouth Disease(FMDV) IgG 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 相关产品: 产品名称 英文名称 货号 规格 猪瘟抗体(IgG) ELISA Kit Classical Swine Fever Virus Antibody(IgG) ELISA Kit CSB-E12765p 192T 犬肾上腺素(EPI)ELISA Kit Canine Epinephrine/Adrenaline,EPI ELISA Kit CSB-E12761c 96T 犬髓过氧化物酶(MPO)ELISA Kit Dog Myeloperoxidase (MPO) ELISA kit CSB-E12762c 96T 猪口蹄疫抗体(IgM) ELISA Kit Pig Antibody against Foot and Mouth Disease(FMDV) IgM ELISA kit CSB-E12768p 192T 猪瘟抗体(IgM) ELISA Kit Anti-CSFV Antibody (IgM) ELISA Kit CSB-E12766p 192T 犬促肾上皮质激素释放激素(CRH)ELISA Kit Dog corticotropin releasing hormone,CRH ELISA Kit CSB-E12763c 96T 大鼠P0蛋白 ELISA kit Rat myelin protein zero (p0) ELISA Kit CSB-E12769r 96T 犬皮质酮/肾上腺酮(CORT)ELISA Kit Canine Corticosterone,CORT ELISA Kit CSB-E12760c 96T
商机
2013.04.17
产品名称: 猪蓝耳病毒(PRRSV)抗体(IgG)ELISA kit 英文名称: Pig reproductive and respiratory syndrome virus(PRRSV)antibody(IgG)ELISA kit 货号: CSB-EQ027198PI 价格: 5000 规格: 192T 种属: Pig 待测物名称: reproductive and respiratory syndrome virus(PRRSV)antibody(IgG) 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use.
商机
2013.04.17
产品名称: 大鼠白介素6(IL-6)ELISA KIT 英文名称: Rat Interleukin 6,IL-6 ELISA KIT 别名: BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2 货号: CSB-E04640r 价格: 3200 规格: 96T 种属: Rat 待测物名称: interleukin 6 (interferon, beta 2) 缩写: IL6 样本类型: serum, plasma, cell culture supernates, tissue homogenates, cell lysates 检测范围: 0.312 pg/ml-20 pg/ml 灵敏度: 0.078 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 标准曲线: 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Rat IL6. No significant cross-reactivity or interference between Rat IL6 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 猪白介素6(IL-6)ELISA Kit Pig Interleukin 6,IL-6 ELISA Kit CSB-E06786p 96T 人白介素6(IL-6)ELISA KIT Human Interleukin 6,IL-6 ELISA KIT CSB-E04638h 96T 牛白介素6(IL-6)ELISA Kit Bovine Interleukin 6,IL-6 ELISA Kit CSB-E12899B 96T 人超敏白介素6(hs-IL6) ELISA kit Human high sensitivity interleukin 6 (hs-IL6) ELISA kit CSB-EQ011664HU 96T 绵羊白介素6(IL-6)ELISA Kit Sheep Interleukin 6,IL-6 ELISA Kit CSB-E10116Sh 96T 小鼠白介素6(IL-6)ELISA Kit Mouse Interleukin 6,IL-6 ELISA KIT CSB-E04639m 96T 猫白介素6(IL-6)ELISA Kit Cat Interleukin 6(IL-6) ELISA Kit CSB-E14046Fe 96T 鸡白介素6(IL-6)ELISA Kit Chicken Interleukin 6,IL-6 ELISA Kit CSB-E08549Ch 96T 马白介素6(IL-6)ELISA Kit Horse Interleukin 6(IL-6)ELISA Kit CSB-E16634Hs 96T 兔子白介素6(IL-6)ELISA Kit rabbit Interleukin 6,IL-6 ELISA Kit CSB-E06903Rb 96T 猴白介素6(IL-6)ELISA Kit Monkey Interleukin 6,IL-6 ELISA Kit CSB-E10050Mo 96T 山羊白介素6(IL-6)ELISA Kit Goat Interleukin 6,IL-6 ELISA Kit CSB-E14360G 96T 犬白介素6(IL-6)ELISA Kit Dog Interleukin 6,IL-6 ELISA Kit CSB-E11260c 96T 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Marmota monax Interleukin-6(IL6) 1mg Recombinant Sparus aurata Interleukin-6(il6) 1mg Recombinant Mustela putorius furo Interleukin-6(IL6) 1mg Recombinant Cercocebus atys Interleukin-6(IL6) 1mg Recombinant Mustela vison Interleukin-6(IL6) 1mg Recombinant Rat Interleukin-6(Il6) 1mg Recombinant Macaca fascicularis Interleukin-6(IL6) 1mg Recombinant Mouse Interleukin-6(Il6) 1mg Recombinant Human Interleukin-6(IL6) 1mg Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6) 1mg Recombinant Cat Interleukin-6(IL6) 1mg Recombinant Sheep Interleukin-6(IL6) 1mg Recombinant Pig Interleukin-6(IL6) 1mg Recombinant Dog Interleukin-6(IL6) 1mg Recombinant Bovine Interleukin-6(IL6) 1mg
商机
2013.04.15
产品名称: 人白介素6(IL-6)ELISA KIT 英文名称: Human Interleukin 6,IL-6 ELISA KIT 别名: BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2 货号: CSB-E04638h 价格: 3600 规格: 96T 种属: Human 待测物名称: interleukin 6 (interferon, beta 2) 缩写: IL6 样本类型: serum, plasma, cell culture supernates, urine, tissue homogenates 检测范围: 7.8 pg/ml-500 pg/ml 灵敏度: 1.95 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Human IL6. No significant cross-reactivity or interference between Human IL6 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 猪白介素6(IL-6)ELISA Kit Pig Interleukin 6,IL-6 ELISA Kit CSB-E06786p 96T 牛白介素6(IL-6)ELISA Kit Bovine Interleukin 6,IL-6 ELISA Kit CSB-E12899B 96T 人超敏白介素6(hs-IL6) ELISA kit Human high sensitivity interleukin 6 (hs-IL6) ELISA kit CSB-EQ011664HU 96T 绵羊白介素6(IL-6)ELISA Kit Sheep Interleukin 6,IL-6 ELISA Kit CSB-E10116Sh 96T 大鼠白介素6(IL-6)ELISA KIT Rat Interleukin 6,IL-6 ELISA KIT CSB-E04640r 96T 小鼠白介素6(IL-6)ELISA Kit Mouse Interleukin 6,IL-6 ELISA KIT CSB-E04639m 96T 猫白介素6(IL-6)ELISA Kit Cat Interleukin 6(IL-6) ELISA Kit CSB-E14046Fe 96T 鸡白介素6(IL-6)ELISA Kit Chicken Interleukin 6,IL-6 ELISA Kit CSB-E08549Ch 96T 马白介素6(IL-6)ELISA Kit Horse Interleukin 6(IL-6)ELISA Kit CSB-E16634Hs 96T 兔子白介素6(IL-6)ELISA Kit rabbit Interleukin 6,IL-6 ELISA Kit CSB-E06903Rb 96T 猴白介素6(IL-6)ELISA Kit Monkey Interleukin 6,IL-6 ELISA Kit CSB-E10050Mo 96T 山羊白介素6(IL-6)ELISA Kit Goat Interleukin 6,IL-6 ELISA Kit CSB-E14360G 96T 犬白介素6(IL-6)ELISA Kit Dog Interleukin 6,IL-6 ELISA Kit CSB-E11260c 96T 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Marmota monax Interleukin-6(IL6) 1mg Recombinant Sparus aurata Interleukin-6(il6) 1mg Recombinant Mustela putorius furo Interleukin-6(IL6) 1mg Recombinant Cercocebus atys Interleukin-6(IL6) 1mg Recombinant Mustela vison Interleukin-6(IL6) 1mg Recombinant Rat Interleukin-6(Il6) 1mg Recombinant Macaca fascicularis Interleukin-6(IL6) 1mg Recombinant Mouse Interleukin-6(Il6) 1mg Recombinant Human Interleukin-6(IL6) 1mg Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6) 1mg Recombinant Cat Interleukin-6(IL6) 1mg Recombinant Sheep Interleukin-6(IL6) 1mg Recombinant Pig Interleukin-6(IL6) 1mg Recombinant Dog Interleukin-6(IL6) 1mg Recombinant Bovine Interleukin-6(IL6) 1mg
商机
2013.04.15
产品名称: 小鼠白介素6(IL-6)ELISA Kit 英文名称: Mouse Interleukin 6,IL-6 ELISA KIT 别名: BSF2, HGF, HSF, IFNB2, IL-6, B cell stimulatory factor-2 货号: CSB-E04639m 价格: 3200 规格: 96T 种属: Mouse 待测物名称: interleukin 6 (interferon, beta 2) 缩写: IL6 样本类型: serum, plasma, cell culture supernates 检测范围: 1.56 pg/ml-100 pg/ml 灵敏度: 0.39 pg/ml 反应时间: 1-5h 所需样本体积: 50-100ul 检测波长: 450 nm 用途: For research use only. Not for diagnostic use. 原理 : This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured. 特异性: This assay has high sensitivity and excellent specificity for detection of Mouse IL6. No significant cross-reactivity or interference between Mouse IL6 and analogues was observed. 精密度: Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess. 样本搜集及储存: Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles. 检测步骤: Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate. 1. Prepare all reagents, working standards, and samples as directed in the previous sections. 2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C. 3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed. 4. Remove the liquid of each well, don't wash. 5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.) 6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels. 7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C. 8. Repeat the aspiration/wash process for five times as in step 6. 9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light. 10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing. 11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate. 结果计算: Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web. Average the duplicate readings for each standard and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the IL6 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 相关产品: 产品名称 英文名称 货号 规格 猪白介素6(IL-6)ELISA Kit Pig Interleukin 6,IL-6 ELISA Kit CSB-E06786p 96T 人白介素6(IL-6)ELISA KIT Human Interleukin 6,IL-6 ELISA KIT CSB-E04638h 96T 牛白介素6(IL-6)ELISA Kit Bovine Interleukin 6,IL-6 ELISA Kit CSB-E12899B 96T 人超敏白介素6(hs-IL6) ELISA kit Human high sensitivity interleukin 6 (hs-IL6) ELISA kit CSB-EQ011664HU 96T 绵羊白介素6(IL-6)ELISA Kit Sheep Interleukin 6,IL-6 ELISA Kit CSB-E10116Sh 96T 大鼠白介素6(IL-6)ELISA KIT Rat Interleukin 6,IL-6 ELISA KIT CSB-E04640r 96T 猫白介素6(IL-6)ELISA Kit Cat Interleukin 6(IL-6) ELISA Kit CSB-E14046Fe 96T 鸡白介素6(IL-6)ELISA Kit Chicken Interleukin 6,IL-6 ELISA Kit CSB-E08549Ch 96T 马白介素6(IL-6)ELISA Kit Horse Interleukin 6(IL-6)ELISA Kit CSB-E16634Hs 96T 兔子白介素6(IL-6)ELISA Kit rabbit Interleukin 6,IL-6 ELISA Kit CSB-E06903Rb 96T 猴白介素6(IL-6)ELISA Kit Monkey Interleukin 6,IL-6 ELISA Kit CSB-E10050Mo 96T 山羊白介素6(IL-6)ELISA Kit Goat Interleukin 6,IL-6 ELISA Kit CSB-E14360G 96T 犬白介素6(IL-6)ELISA Kit Dog Interleukin 6,IL-6 ELISA Kit CSB-E11260c 96T 蛋白 产品名称 货号 规格 蛋白来源 Recombinant Marmota monax Interleukin-6(IL6) 1mg Recombinant Sparus aurata Interleukin-6(il6) 1mg Recombinant Mustela putorius furo Interleukin-6(IL6) 1mg Recombinant Cercocebus atys Interleukin-6(IL6) 1mg Recombinant Mustela vison Interleukin-6(IL6) 1mg Recombinant Rat Interleukin-6(Il6) 1mg Recombinant Macaca fascicularis Interleukin-6(IL6) 1mg Recombinant Mouse Interleukin-6(Il6) 1mg Recombinant Human Interleukin-6(IL6) 1mg Recombinant Macaca mulatta (Rhesus macaque) Interleukin-6(IL6) 1mg Recombinant Cat Interleukin-6(IL6) 1mg Recombinant Sheep Interleukin-6(IL6) 1mg Recombinant Pig Interleukin-6(IL6) 1mg Recombinant Dog Interleukin-6(IL6) 1mg Recombinant Bovine Interleukin-6(IL6) 1mg
商机
2013.04.15
CUSABIO品牌ELISA试剂盒,礼品大赠送!!! CUSABIO品牌ELISA试剂盒,礼品大赠送!!! 飞轩生物携手CUSABIO品牌! 飞轩生物将秉承公司为您提供“最高质量的产品”和“最优质的服务”的宗旨 为广大科研工作者服务! 即日起 凡购买CUSABIO品牌ELISA试剂盒 既有机会获得精美礼品 考虑到礼品的实用性 我们精心为您准备了: 派克水笔 定时器!! 礼品多多,机会属于你!! 本活动解释权---上海飞轩生物科技有限公司
厂商
2013.04.08