全自动外泌体荧光检测分析系统

全自动外泌体荧光检测分析系统

粒径分析

全自动外泌体荧光检测分析系统能够对>50 nm的外泌体进行全方面的表征，无论是粒径尺寸、粒径分布还是外泌体的亚型均可在一次测试中得到。并且所用来测试的样本无需进行纯化，避免因纯化带来的样本偏差。

分析不同大小和不同尺度的外泌体亚群，在CD171过表达系统中，100 nm以上的外泌体仅表达了CD171。

量化外泌体亚群 

通过抗体捕获的模式，全自动外泌体荧光检测分析系统能够量化含有不同标记物的外泌体亚群，并对不同种群的外泌体进行特异性计数和分析。整个过程无需纯化，并且线性范围可跨越3个数量。

TSPAN8阳性细胞外囊泡的稀释曲线。使用1:3比例的梯度稀释。用R² = 0.9986计算与TSPAN8荧光数据的线性拟合。

检测外囊泡中的装载物 

MISEV建议在表征外泌体和外囊泡时，应当同时测量表面和载体蛋白。使用ExoView芯片可穿透外泌体，探测膜内蛋白和载体。并且单次可定量3种表面、管腔蛋白。

检测细胞外泌体内的Syntenin。

WT细胞在未穿膜的条件下几乎观测不到Syntenin的信号。进行穿膜处理后可以观测到Syntenin信号，而KO之后信号消失

生物标志物共定位 

全自动外泌体荧光检测分析系统能够在单个外泌体样本上检测多达4种标记物，并同时提供外泌体的其它表征数据诸如计数、颗粒尺寸统计等。

无需纯化

全自动外泌体荧光检测分析系统只测量含有靶标抗原的特定细胞外泌体群体。仅需35ul的稀释样品，即可直接获得外泌体的表型、l粒径和计数信息。并且无需担心污染物对测试的影响。

无论纯化与否，Exoview均可进行分析

ExoView™ Kit 配置清单:

用户单位

中国用户已发表文章

☛ 南京大学李靓/张辰宇/张玉婧课题组在《Frontiers in Cell and Developmental Biology》发表文章。

☛ 上海大学在《Journal of extracellular vesicles》发表文章。

☛  中国科学院深圳技术研究院在《Lab on a Chip》发表文章。

☛  北京天坛医院、国家纳米科学中心、北京航空航天大学在《Advanced Science》发表文章。

☛  同济大学附属上海市肺科医院、上海思路迪转化医学在《Journal of Nanobiotechnology》发表文章。

☛ 山东千佛山医院在《NANO LETTERS》发表文章

感谢用户老师对Quantum Design China & NanoView设备的认可与支持！

2022-2023发表文章

• Richard J. R. Kelwick …& Paul S. Freemont. (2023) Opportunities to accelerate extracellular vesicle research with cell-free synthetic biology. Journal of Extracellular Vesicles.

• Laurence Blavier …& Yves A. DeClerck. (2023) The capture of extracellular vesicles endogenously released by xenotransplanted tumours induces an inflammatory reaction in the premetastatic niche. Journal of Extracellular Vesicles.

• Hongyun Wang ……& Junjie Xiao. (2022) Extracellular vesicles enclosed-miR-421 suppresses air pollution (PM2.5)-induced cardiac dysfunction via ACE2 signalling. Journal of Extracellular Vesicles.

• Linglei Jiang……& Santosh Dhakal. (2022) A7 bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against disease and elicits neutralizing antibodies to wild-type and Delta variants. Journal of extracellular vesicles.

• Shaobo Ruan, Nina Erwin, Mei He. (2022) Light-induced high-efficient cellular production of immune functional extracellular vesicles. Journal of extracellular vesicles.

• Nasibeh Karimi ……&  Cecilia Lässer. (2022) Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples. Journal of Extracellular Vesicles.

• Heikki Kyykallio ……& Pia R-M Siljander. (2022) A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles. Journal of Extracellular Vesicles.

• Roberto Frigerio ……& Marina Cretich. (2022) Comparing digital detection platforms in high sensitivity immune-phenotyping of extracellular vesicles. Journal of Extracellular Vesicles.

• Yael Hirschberg ……& Inge Mertens. (2022) Characterizing extracellular vesicles from individual low volume cerebrospinal fluid samples, isolated by SmartSEC. Journal of Extracellular Vesicles.

• Sukhbir Kaur……& David D. Roberts. (2022) Single vesicle analysis of CD47 association with integrins and tetraspanins on extracellular vesicles released by T lymphoblast and prostate carcinoma cells. Journal of Extracellular Vesicles.

• Simone M. Crivelli……& Erhard Bieberich. (2022) Function of ceramide transfer protein for biogenesis andsphingolipid composition of extracellular vesicles. Journal of Extracellular Vesicles.

• Gi Beom Kim …& In-San Kim. (2023) Harnessing Oncolytic Extracellular Vesicles for Tumor Cell-Preferential Cytoplasmic Delivery of Misfolded Proteins for Cancer Immunotherapy. Small.

• J. Klein, Y. Cao. (2023) Multi-Lipid Synergy In Cartilage Lubrication: Redundancy Or Evolution? Osteoarthritis and Cartilage.

Valerie DeLuca …& Michael E. Berens. (2023) Extracellular vesicles as a pharmacodynamic reporter in glioblastoma. Cancer Research.

• Sarah Pike …& Liya Xu. (2023) Extracellular vesicles in diagnostic retinoblastoma aqueous humor correlate with disease stage and clinical outcome: a pilot study. Cancer Research.

• D. Cookson …& Y. Ashraf Kharaz. (2023) The Biological Response Of Anterior Cruciate Ligamentocytes Following Treatment With Synovial Fluid Extracellular Vesicles. Osteoarthritis and Cartilage.

• Mei Lu …& Yuanyu Huang. (2023) Antitumor synergism between PAK4 silencing and immunogenic phototherapy of engineered extracellular vesicles. Acta Pharmaceutica Sinica B.

• Luke C. McIlvenna ……& Martin Whitham. (2023) Single vesicle analysis reveals the release of tetraspanin positive extracellular vesicles into circulation with high intensity intermittent exercise. The Journal of Physiology.

• Joseph Blommer ……& Dimitrios Kapogiannis. (2023) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. BRAIN.

• Tyler J ……& Atta Behfar. (2022) Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence. Npj Regenerative Medicine.

• Min Han ……& Tao Xin. (2022) Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair. Nano Letters.

• Zijian Yang ……& David A. Issadore. (2022) Ultrasensitive Single Extracellular Vesicle Detection Using High Throughput Droplet Digital Enzyme-Linked Immunosorbent Assay. Nano Letters.