高通量4D-蛋白质组学： dia-PASEF®和Evosep One结合的短梯度应用

Lipid profiling from complex lipid extracts can be a challenging and time consuming task. The high complexity of samples and co-elution of isobaric or isomeric compounds complicate the confident annotation of lipids. The presented 4D-Lipidomics workflow simplifies and streamlines the annotation and validation process using mobility-enhanced MS data.

However, the dynamic range of protein concentration in these samples poses a challenge for in-depth proteome quantification. PASEF® is a powerful technology for rapid in-depth and sensitive proteome quantification that is also applicable to biofluids like plasma and serum. We combined PASEF with high-throughput gradient settings for the analysis of hundreds of plasma and serum samples and quantified several hundred protein groups routinely in 21- and 47-minute runtimes. The timsTOF Pro coupled with the Evosep One nanoflow UPLC delivers quantitation with median CV less than 20% and quantified more than 70 known biomarkers in a study investigating over 700 COVID-19 serum samples

The combination of integrated ion mobility with warp-speed MS/MS acquisition on the timsTOF Pro paves the way for deep, molecular level understanding of β-cell physiology and potential new targetable pathways for diabetes.

MALDI Guided SpatialOMx，即基于质谱成像定位的空间多组学分析，完美的将MALDI成像技术和传统LC-MS/MS组学流程结合在了一起，它的特点是利用了MALDI成像技术能在分子原位对生物分子进行定位的特点，在生物组织的内部锁定目标微区（ROI，Region of Interests），在对该目标微区实施激光微切割（LCM，laser capture microdissection）后，进行LC-MS/MS组学分析，从而实现了深度的蛋白组信息挖掘。