空间定位组学对肿瘤亚细胞群的深度蛋白组分析

Lipid profiling from complex lipid extracts can be a challenging and time consuming task. The high complexity of samples and co-elution of isobaric or isomeric compounds complicate the confident annotation of lipids. The presented 4D-Lipidomics workflow simplifies and streamlines the annotation and validation process using mobility-enhanced MS data.

However, the dynamic range of protein concentration in these samples poses a challenge for in-depth proteome quantification. PASEF® is a powerful technology for rapid in-depth and sensitive proteome quantification that is also applicable to biofluids like plasma and serum. We combined PASEF with high-throughput gradient settings for the analysis of hundreds of plasma and serum samples and quantified several hundred protein groups routinely in 21- and 47-minute runtimes. The timsTOF Pro coupled with the Evosep One nanoflow UPLC delivers quantitation with median CV less than 20% and quantified more than 70 known biomarkers in a study investigating over 700 COVID-19 serum samples

The combination of integrated ion mobility with warp-speed MS/MS acquisition on the timsTOF Pro paves the way for deep, molecular level understanding of β-cell physiology and potential new targetable pathways for diabetes.

最新一代布鲁克timsTOF fleX使用创新的激光后电离MALDI-2技术获得了较传统MALDI 高1-3个数量级的灵敏度。MALDI-2提高了离子产率并降低了离子抑制效应。同时，MALDI-2还可对传统MALDI不能电离的药物和代谢物分子检测和成像，如甾醇、他汀类、激素、维生素、聚糖等生物分子。这种实质性的改进对于DMPK研究尤其重要。