人原钙黏素ELISA试剂盒洗板两点方法对收集后当天进行检测的标本，储存在4℃备用，如有特殊原因需要周期收集标本，将标本及时分装后放在-20℃或-70℃前提下保留。因为“人原钙黏素1 ELISA试剂盒具有的特异性，抗原抗体的结合发生在抗原的决定簇与抗体的结合位点之间。 -70℃可保留6个月。标本2-8℃可保留48小时，-20℃可保留1个月。部门激素类标本需添加抑肽酶。那么人原钙黏素1 ELISA试剂盒洗板方法有以下两点：1. 自动洗板：假如有自动洗板机，应在纯熟使用后再用到正式实验过程中。 2. 手工洗板方法：吸去(不可触及板壁)或甩掉酶标板内的液体；在实验台上铺垫几层吸水纸，酶标板朝下用力拍几回；将推荐的洗涤缓冲液至少0.3ml注入孔内，浸泡1-2分钟，根据需要，重复此过程数次。待检样本：体液、血清、血浆、细胞培养上清液、尿液、组织匀浆、心房水标本等。人原钙黏素ELISA试剂盒的样品采集方法，是收集标本前必需清晰要检测的成份是否足够不乱。避免反复冻融

代谢组学之父尼克尔森：中国仅有基因组研究是不够的近日，全球代谢组学之父Jeremy K. Nicholson教授在上海交通大学医学院附属瑞金医院举办的研讨会上，与中国科学家共同探讨“代谢表型研究与健康和医疗的全新未来”。Nicholson在研讨会上发表的观点独具亮点。从代谢组学研究出发，他特别提到了快速蒸发电离质谱技术（REIMS）在临床诊断中的应用。通过这项化学活性工具，外科医生有望在手术中实现实时诊断，从而把肿瘤切除干净。这项技术概念在临床上被亲切地称为“智能刀”。对中国的人类基因组项目，Nicholson认为，一方面，中国启动了最大的人类基因组项目；另一方面，仅有基因组的研究是不够的。表型组学是解读基因组非常必要的学科。到目前为止，中国的基因组项目中已获得了一些有关生理特点、表型特点的描述，现在所缺少的数据正是代谢表型相关的一些数据。他认为，补足这部分缺失的数据是中国的人类表型组项目下一步的重要工作之一。Nicholson是代谢组学领域的先驱和国际顶尖科学家，现任英国帝国理工学院表型组研究中心负责人、外科与癌症部主管、消化与超导健康研究中心主任。他于1999年首次提出了代谢组学的定义，在《自然》杂志等学术刊物上发表论文500多篇。2013年Nicholson教授被聘为中国科学院“爱因斯坦讲席教授”。据悉，Nicholson教授此前还在北京参加了中科院组织的香山科学会议，并作了《人类表型研究》相关主题报告

中国“雷公藤”植物能解决所有肥胖问题吗？近几十年来，科学家遍寻世界神奇植物尝试所有方法让人们变得更苗条。当减肥产品和减肥补充剂的市场发展成为几十亿美元的产业时，他们开始研究蒲公英、咖啡、坚果等等。科学家们种植了一种可食用的肉质植物--印度仙人掌，印度农村的部落人民在打猎时食用印度仙人掌来抑制饥饿。科学家们来尝试萃取一种名叫火地亚属（hoodia)非洲植物的精华，这种看起来像细长的泡菜的植物会让你产生饱腹感，即使你一口也没开始吃。但是在早期的研究中，没有一种植物比中国中草药-雷公藤在减肥方面更能大放异彩。在一篇发表在《细胞》周四期刊的文章里，科学家声称有一种从植物中萃取的物质可以降低食物的摄取量，并且在实验中戏剧性地使一只肥老鼠的体重降低了45%。[科学家：两种商业减肥计划能成功让你的体重下降]文章的作者，Omut Ozan， 波士顿儿童医院以及哈佛医学院的内分泌医生，称这种物质是通过提高一种脂源性的荷尔蒙--瘦素起作用的，瘦素在身体摄入足够多燃料和能量时会向身体发出信号。缺少瘦素的人，会陷入不节制的饮食中，导致变态性肥胖。"在最近二十几年里，人们付出了巨大的努力试图分解瘦素抵抗 来治疗肥胖，但是这些尝试都失败了。而这个研究透露仍有希望让瘦素在减肥中发挥作用的信息“Ozcan 在一次声明中说到。研究中，Ozcan发现在采用雷公藤的萃取物--他们称为南蛇藤醇，治疗一个礼拜后的老鼠食物摄取量比没有采取治疗的老鼠降低了80%。三个礼拜后，这些摄取雷公藤的萃取物的老鼠体重比最初的体重减少了一半。研究成果比采用激烈的减肥手段--肥胖手术更有效。另外，科学家声称除了减肥效果他们还发现其他正面的健康效果，包括降低胆固醇以及改善肝功能。[  摘掉Fitbit(智能手环）吧，只运动是不会让你减肥成功的。]然而，研究团队在老鼠身上没有发现这种萃取物的任何毒性效应，研究人员警告说需要做更多的研究去证明这种化合物作用在人体上的安全性。“在雷公藤的根部发现少量的南蛇藤醇，但是这种植物的根部以及花朵发现很多其他的化合物，“Ozcan 说道，”因此，这种植物可能对人类是有害的。

长生不老还有超能力：那些让你脑洞大开的黑科技脑洞大开，想“长生不老”的各位亲不用再苦等唐僧肉了！科学家说200年内人类可以通过生物科技和遗传工程学技术，将自己打造成半机械人，从而自己控制生死。但是！愿望是美好的，资金是巨额的。不过，梦想还是要有的，万一有钱了呢？【磁力植入：瞬间拥有超能力】感知周围事物的各种运动、捡起细小的金属物体、徒手诊断电池......或许在未来你也可以变身拯救人类的蜘蛛人。磁体通常被植入手掌或手指，使得用户可以感受到附近的磁场和微波。当某物体正在接近自己时，你便会感觉到震动；还能捕捉声纳、紫外线、WiFi和热量数据，并将这些肉眼无法看到的各种场的相关信息，传送给植入体内的磁体。【头部移植？Get√】只有想不到，没有做不到！大脑是人体最为精密的器官，把捐献者的头移植到患者身上。单这么想一下，就感觉头都大了。不过意大利都灵高级神经协调研究组织的Sergio Canavero认为，头部移植手术能够在两年内实现，这项技术能够拯救遭受癌症折磨的病患生命，以及那些神经和肌肉萎缩的患者。【人体隐身变色龙：你看不到我！】世界上最遥远的距离不是生与死，而是我站在你面前而你看不到我。这就是美国正在努力研制的可随环境变化的人体“隐身衣”，有望18个月之内测试首个原型。它能适应各种温度，以及雨雪天气。如果这种适应性隐身衣需要电源，重量不会超过0.45公斤，并且能够持续工作8个小时。【智能纹身：酷的根本停不下来！】智能纹身不再是一种单纯彩绘，它能让你享受更多炫酷体验！检测人体活动和心跳只是它的基本功能，它还能探测到你是否处于患病前兆。还是不够强大？伊力诺依大学已经成功研制出了一种可以起到类似NFC芯片作用的纹身。试想一下，开机、进门只需抬手通过纹身识别开启，移动支付只需纹身提供密钥，是不是酷的停不下来？【能上网的隐形眼镜：一眼看穿你】还在羡慕谷歌眼镜？too young too naive。华盛顿大学帕尔维兹教授预测能上网的隐形眼镜2030年前就会出现。这种隐形眼镜上排列着一个LED集合，能进行面部识别，显示所见者的生平，还能自动翻译镜片上的字幕。见陌生人戴上它，分分钟就能了解对方。

IgM抗体ELISA在钩端螺旋体病流行地区，已被推荐为快速诊断钩端螺旋体病的免疫球蛋白的M酶联免疫吸附试验（IgM抗体ELISA）得到了广泛的应用。我们进行了一项回顾性病例对照研究，其表现如下：218例（确诊病例109钩端螺旋体病的显微镜凝集试验和109名对照患者的急性发热性疾病）来评估一个的商业IgM抗体ELISA（PANBIO）在泰国东北部诊断的准确率。    入院至少10天之后出现症状的配对血清样品进行了测试。制造商（11 PANBIO单位）推荐使用的临界值，双份血清IgM抗体ELISA的敏感性和特异性分别为90.8％和55.1％。使用的接收机工作特性曲线来恒定，以确定最佳的截止值。这是的20 PANBIO的单位，其敏感性和特异性分别为76.1％和82。

近日，来自美国埃默里大学的研究人员发现一种治疗肺癌的方法，他们利用小分子化合物诱导细胞凋亡从而达到治疗目的，这种小分子能够将BCL2从一个抗凋亡因子变为促凋亡因子，具有重大应用前景。 肺癌是美国癌症相关死亡的头号杀手，因肺癌死亡的人数比乳腺癌，前列腺癌和胰腺癌死亡人数的总和好要多。抗凋亡因子BCL-2家族成员表达上调以及促凋亡家族成员的表达紊乱导致人类肺癌发生化疗抵抗和放疗抵抗的重要因素，这表明BCL-2家族成员可能称为靶向治疗肺癌的关键作用靶点。 BCL-2的BH4结构域对于BCL-2发挥抗凋亡功能具有重要作用，在该项研究中，研究人员通过小分子筛选发现一个靶向BCL2-BH4结构域的拮抗小分子，BDA-366,它能够与BH4结构域结合，同时具有高度的亲和性和特异性。BDA-366与BCL-2结合能够诱导BCL-2发生构象改变，减弱BCL-2的抗凋亡功能，并将其从一个维持细胞存活的蛋白分子转变为一个细胞凋亡诱导因子。BDA-366能够抑制肺癌细胞系以及肺癌病人来源的畸胎瘤的生长，同时对正常组织没有明显的毒副作用。除此之外，研究人员还发现在肺癌细胞以及肺癌病人肿瘤组织中抑制mTOR能够诱导BCL-2表达；将BDA-366与RAD001联合使用治疗肺癌具有很强的协同性。 因此，开发BCL2-BH4拮抗剂能够为肺癌治疗提供有效的治疗策略，对于提高肺癌病人生存率，攻克肺癌病魔具有重要意义。hz-2137R Influenza A virus (Duck)  鸭流感病毒抗体hz-2150R TNF-alpha  肿瘤坏死因子-α抗体hz-1603R AARS2  丙氨酰tRNA合成酶2抗体hz-2185R TDRD9/HIG1  缺氧诱导蛋白HIG1抗体hz-2257R SIRT1/sirtuin 1  沉默调节蛋白1抗体hz-2321R Spindly/CCDC99  亚砷盐相关蛋白抗体hz-2354R TBX-5  转录因子Tbx5抗体hz-0096R AAT/Tryptase  α-1抗胰蛋白酶抗体hz-1510R AATK  AATK细胞凋亡关联酪氨酸激酶抗体hz-2451R NMP-22  核基质蛋白22hz-1229R AATF  拮抗凋亡转录因子抗体hz-1627R ABCA1/ABC1  腺苷三磷酸结合盒转运体A1抗体hz-1761R ABCD1/CCL22  嗜酸粒细胞趋化蛋白22抗体hz-1604R ABCB5  ATP结合蛋白家族5抗体hz-1224R ABCB6  ATP结合蛋白家族6抗体hz-1960R ABCF1  ATP结合盒蛋白家族GCN20F家族1抗体hz-1231R ABCG1  三磷酸腺苷结合盒亚家族G1抗体hz-0662R ABCG2/CD338  三磷酸腺苷结合转运蛋白G超家族成员2抗体hz-2812R KAT4  细胞周期基因1蛋白抗体hz-1727R ABCG4  ABC膜转运蛋白抗体hz-5013R ABCG5  三磷酸腺苷结合转运蛋白G超家族成员5抗体hz-5014R ABCG8  三磷酸腺苷结合转运蛋白G超家族成员8抗体hz-0583R c-Abl/Abl1  非受体酪氨酸激酶c-Abl抗体hz-4083R phospho-c-Abl(Tyr134)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-3032R phospho-c-Abl(Tyr412)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-3041R phospho-c-Abl(Tyr245)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-3042R phospho-c-Abl(Tyr204)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-3044R phospho-c-Abl(Ser735)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-3071R phospho-c-Abl(Tyr89)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-4084R phospho-c-Abl(Tyr251)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-4085R phospho-c-Abl(Tyr272)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-4086R phospho-c-Abl(Tyr276)  磷酸化非受体酪氨酸激酶c-Abl抗体hz-4087R SPTLC1  丝氨酸棕榈酰转移酶1抗体hz-4088R SLC27A5  脂肪酸转运蛋白5抗体hz-2932R Clathrin heavy chain/Clathrin HC  网格蛋白重链抗体

最近免疫疗法的崛起令我思考化疗这个最早癌症治疗策略是否因为误伤了免疫系统而帮了不少倒忙。化疗是癌症治疗的一个重要里程碑，通过化学品人类第一次使无法手术（如白血病）的肿瘤短暂消失。化疗的基本思路是杀死一切快速分裂的细胞，癌细胞肯定属于这个范畴。但早期的化疗并没有意识到参与清除肿瘤的免疫细胞也是快速分裂的细胞之一。化疗药物好比炮弹无选择地杀死一部分犯罪分子同时也误伤一些快速反应部队，所以净结果并不显着。如果化疗药物只是细胞毒，那么似乎这些药应该都是抑制免疫系统的。事实上化疗药物最主要的副作用之一就是白细胞的下降，所以很多化疗药物的确是抑制免疫系统的，很多早期化疗药物如环磷酰胺和甲氨蝶呤本身就作为免疫抑制剂用于免疫疾病的治疗。直接杀死免疫细胞只是抑制免疫系统的机理之一，还有其它方式可以影响免疫系统。比如BCR-ABL激酶抑制剂格列卫（严格说靶向疗法不能算是化疗）可以通过抑制T细胞活化的关键酶LCK（也是一种激酶）而选择性抑制记忆T细胞分化，而抑制免疫系统。化疗导致大量细胞死亡本身也会抑制免疫系统和诱导免疫耐受，这里面又涉及几个不同途径。不仅化疗，肿瘤外科医生也经常摘除病人的淋巴结以避免转移，但淋巴结是T细胞成熟和分化的地方，所以不可避免地降低免疫系统的抗癌作用。这种盲人摸象式的探索居然能治愈一些病人也是个奇迹，当然免疫系统对一些肿瘤本来就无能为力外，抑制了也无大碍。也有一些化疗药物通过当时并未意识到的机理激活免疫系统。比如DNA损伤药物如拓扑酶抑制剂可以通过激活p53而引发一系列生物过程诱导免疫原性。蒽环类药物可以增加肿瘤抗原表达，而低浓度环磷酰胺则可以选择性地抑制调解性T细胞（本身抑制免疫系统，所以降低这类T细胞反而激活免疫系统）。紫杉醇类则可以直接激活T细胞。最近上市的CDK4/6抑制剂Ibrance虽然只抑制细胞的分裂过程，但如果肿瘤细胞在某个阶段停滞时间过长则会引起免疫系统的注意，遭到清理。所以Ibrance不仅是cytostatic还有细胞毒疗效。化疗设计之初的想法是比较简单的，对免疫系统损失对抗衡肿瘤的不利后果并没有准确估计。现在我们对免疫系统在清除肿瘤细胞的功能有了长足的进步，根据化疗药物对免疫系统的不同作用而设计免疫疗法和化疗的复方组合也正在成为一个快速发展的新方向。化疗这个相对古老的技术可能通过和免疫疗法的合作开始显示真正的威力。免疫系统只是生物体系诸多复杂因素之一。生物体系正负反馈机制导致的受体失敏，代替机能的激活使很多药物疗效大打折扣。很多罕见毒副作用现在很难预测和解释。这些也提醒我们虽然人类有了智能手机和航天飞船但新药设计还在萌芽期。但技术的进步也是令人目不暇接，希望肿瘤很快会象天花一样成为医学史上的插曲。hz-0253R ER α 雌激素受体α抗体hz-0263R CKLFSF2/CMTM2 趋化素样因子超家族成员2抗体hz-0264R COX7A2 细胞色素c氧化酶VIIA亚型2抗体hz-0265R CKLFSF2 isoform 1 趋化素样因子超家族成员2亚型1抗体hz-0266R MAP65/ASE 1  拟南芥MAP65蛋白抗体hz-0437R Streptavidin/SA protein  链酶亲和素抗体hz-0474R human Serum IgA 人血清型免疫球蛋白A抗体hz-0494R ETFA  电子转移黄素蛋白α抗体hz-0631R γ-taxilin/LSR5 脂多糖相关反应蛋白5抗体hz-0706R T4/L-Thyroxine 甲状腺素T4抗体hz-0750R HLA G(N-Terminus) 人类白细胞抗原G抗体（N端）hz-0753R HLA G(C-terminal) 人类白细胞抗原G抗体（C端）hz-0887R hhzagglutinin protein/H  麻疹病毒血凝素抗体hz-0957R WIG-1/PAG608  野生型P53诱导基因1抗体hz-0988R CD71/TFR/Transferrin receptor  转铁蛋白受体抗体hzm-0978M GAPDH(3E12)-Loading Control  3-磷酸甘油醛脱氢酶单克隆抗体(内参抗体)hz-2188R GAPDH   3-磷酸甘油醛脱氢酶抗体(内参抗体)hz-0061R beta-Actin (Loading Control)  β-肌动蛋白抗体（内参抗体）hz-0237R 14-3-3(Alpha/Beta/Gamma/Delta/Epsilon)  14-3-3蛋白抗体hz-3000R phospho-14-3-3 protein zeta/delta(Ser58)  磷酸化14-3-3 α/β/ζ抗体hz-2017R 14-3-3 family protein  苹果14-3-3蛋白抗体(植物)hz-1206R GLI1/Zfp5  脑胶质瘤相关蛋白抗体（锌指蛋白5）hz-1236R Involucrin  囊包蛋白/内披蛋白抗体hz-1240G human Fibrinogen  羊抗人纤维蛋白原抗体hz-1248R S100B/S100 beta  S100B蛋白抗体hz-1288G GDF8  生长分化因子8/肌肉抑制素抗体hz-0861R 2,4-D  2，4-二氯苯氧乙酸(除草剂)抗体hzm-0861M 2,4-D(24D1)  2，4-二氯苯氧乙酸(除草剂)单克隆抗体hz-2559R eIF4EBP1/4EBP1  eIF4E结合蛋白抗体hz-3018R phospho-eIF4EBP1/4EBP1(Ser64)  磷酸化4E结合蛋白1抗体hz-3019R phospho-eIF4EBP1(Thr37/46)  磷酸化4E结合蛋白1抗体hz-3720R phospho-eIF4EBP1/4EBP1(Ser65/Thr70)  磷酸化eIF4E结合蛋白抗体hz-5334R phospho-EIF4EBP1(Ser100)  磷酸化eIF4E结合蛋白抗体hz-5337R phospho-EIF4EBP1(Ser111)  磷酸化eIF4E结合蛋白抗体hz-2913R EIF5  真核翻译起始因子5抗体hz-3839R 4EBP2  eIF4E结合蛋白2抗体hz-1440R Cytokeratin 5/CK5  细胞角蛋白5抗体hz-1126R 5-HT  5-羟色胺抗体hz-1597R HRPT2/CDC73/Parafibromin  甲状旁腺功能亢进蛋白2抗体（细胞分裂周期73）hz-1124R 5-HTR1A  5-羟色胺受体1A抗体hz-1663R THOC2  转录因子THOC2抗体hz-1665R VEGF  血管内皮生长因子抗体hz-1677R DNA Ligase IV/LIG4  DNA连接酶4抗体hz-1125R 5-HTR1B 5-羟色胺受体1B抗体hz-1892R 5-HTR2B 5-羟色胺受体2B抗体hz-1056R 5-HTR2A 5-羟色胺受体2A抗体hz-1893R 5-HTT  5-羟色胺转运蛋白抗体hz-2126R 5-HTR3  5-羟色胺受体3抗体hz-2127R 5-HTR4  5-羟色胺受体4抗体hz-0526R 5 lipoxygenase/ALOX5  5-脂氧合酶抗体hz-3251R Phospho-5-Lipoxygenase(Ser271)  磷酸化5-脂氧合酶抗体hz-3252R Phospho-5-Lipoxygenase(Ser663)  磷酸化5-脂氧合酶抗体hz-3874R ALOX12/12 Lipoxygenase  12脂氧合酶抗体hz-2036R Penicillin G  青霉素G抗体hz-1278R 8-OHdG  8-羟基脱氧鸟苷抗体hz-2053R RYBP/APAP1/CD337  凋亡相关蛋白1抗体hz-2054R Nkx2.5/Cardiac-specific homeobox 1  心脏特异性同源盒转录因子NKX2.5抗体hz-4235R ADORA1  腺苷A1A受体抗体hz-2077R AMP deaminase 1  腺苷单磷酸脱氨酶1抗体hz-1456R A2AR/Adenosine A2a receptor  腺苷A2A受体抗体hz-0094R AACT-Alpha1/A1ACT  α-1抗胰糜蛋白酶抗体hz-1507R AAK1  AP2关联激酶1抗体hz-2123R Cyp2-j3  细胞色素P450Ⅱj3抗体hz-2128R OST-beta  有机溶质转运蛋白OSTβ抗体hz-3914R AANAT  芳香胺N-乙酰化转移酶抗体hz-2133R AT2R2  血管紧张素Ⅱ受体2抗体

 人翻译调节肿瘤蛋白(TPT1)ELISA kit产品类型:ELISA Kit产品名称:人翻译调节肿瘤蛋白(TPT1)ELISA kit英文名称:Human Translationally-controlled tumor protein(TPT1) ELISA kit人翻译调节肿瘤蛋白(TPT1)ELISA kit别名:FLJ27337, HRF, TCTP, p02, fortilin|histamine-releasing factor规格:96T人翻译调节肿瘤蛋白(TPT1)ELISA kit种属:Human待测物名称:tumor protein, translationally-controlled 1缩写:TPT1检测范围:12.5 pg/ml-800 pg/ml灵敏度:3.12 pg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nm人翻译调节肿瘤蛋白(TPT1)ELISA kitThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

 人三碘甲状腺原氨酸(T3)ELISA Kit产品类型:ELISA Kit产品名称:人三碘甲状腺原氨酸(T3)ELISA Kit英文名称:Human Tri-iodothyronine,T3 ELISA Kit 人三碘甲状腺原氨酸(T3)ELISA Kit规格:96T人三碘甲状腺原氨酸(T3)ELISA Kit种属:Human待测物名称:Tri-iodothyronine,T3缩写:T3检测范围:0.5 ng/ml-8ng/ml灵敏度:0.25 ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nm人三碘甲状腺原氨酸(T3)ELISA KitThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of T3 in the sample. The color development is stopped and the intensity of the color is measured.

近日，来自比利时的科学家在著名国际学术期刊nature在线发表了一项最新研究进展，他们发现肝细胞生长因子受体MET在中性粒细胞趋化以及发挥杀伤活性方面具有重要作用，但通过药物靶向MET治疗癌症的治疗效果可能因MET在中性粒细胞中的作用而部分抵消。 原癌基因MET的突变或异常表达与多种肿瘤的发生有关，同时MET信号途径的组成型激活对于肿瘤生长和存活具有重要作用。有研究发现MET不仅在癌细胞中表达，在肿瘤相关的间充质细胞中也存在表达，但MET表达在肿瘤相关的间充质细胞中究竟发挥什么作用仍不清楚。 在该项研究中，研究人员发现MET对于中性粒细胞应答干细胞生长因子，产生趋化行为以及发挥细胞毒性具有重要作用。在小鼠中性粒细胞中删除Met会促进肿瘤生长及转移，这一表型与原发肿瘤和肿瘤转移灶中中性粒细胞浸润下降具有相关性。同时，MET对于中性粒细胞在大肠炎，皮疹和腹膜炎等疾病中的渗出也是非常必要的。 随后，研究人员对其中的机制进行了深入探究，他们发现在小鼠和人类中性粒细胞中MET的表达会受到肿瘤来源的TNFα以及其他炎症刺激的诱导，而这种诱导作用对于中性粒细胞跨越活化的内皮细胞以及在肝细胞生长因子刺激下产生诱导型一氧化氮合酶都是有帮助的。因此，由中性粒细胞释放的HGF/MET依赖性一氧化氮能够促进癌细胞杀伤，抑制肿瘤生长和转移。研究人员又通过全身给药的方式给予小鼠MET激酶抑制剂，证明靶向癌细胞中MET的治疗获益会因阻断中性粒细胞MET的肿瘤杀伤作用而发生部分抵消。 综上所述，这项研究发现MET在中性粒细胞发挥肿瘤杀伤作用方面具有重要作用，同时提出利用靶向MET的方法治疗癌症可能会因MET在中性粒细胞中的作用而不能获得理想的治疗效果。hz-E10029 中文名称：人N钙黏蛋白/神经钙黏蛋白(N-Cad)ELISA试剂盒 英文名称：Human Neural-Cadherin, N-Cad ELISAkit 规格：48T/96Thz-E10030 中文名称：人红细胞刺激因子(ESF)ELISA试剂盒 英文名称：Human erythropoiesis stimulating factor,ESF ELISAkit 规格：48T/96Thz-E10031 中文名称：人肿瘤坏死因子相关激活诱导因子(TRANCE)ELISA试剂盒 英文名称：Human TNF related activation induced cytokine,TRANCE ELISAkit 规格：48T/96Thz-E10032 中文名称：人生长激素释放因子(GH-RF)ELISA试剂盒 英文名称：Human growth hormone relasing factor,GH-RF ELISAkit 规格：48T/96Thz-E10033 中文名称：人巨噬细胞趋化因子(MCF)ELISA试剂盒 英文名称：Human macrophage chemotatic factor,MCF ELISAkit 规格：48T/96Thz-E10034 中文名称：人α/β干扰素受体(IFN-α/βR)ELISA试剂盒 英文名称：Human Interferon α/βReceptor,IFN-α/βR ELISAkit 规格：48T/96Thz-E10035 中文名称：人B细胞生长因子(BCGF)ELISA试剂盒 英文名称：Human B cell growth protein,BCGF ELISAkit 规格：48T/96Thz-E10036 中文名称：人B细胞分化因子(BCDF)ELISA试剂盒 英文名称：Human B cell differetiation factor,BCDF ELISAkit 规格：48T/96Thz-E10037 中文名称：人上皮细胞粘附分子(Ep-CAM/CD362)ELISA试剂盒 英文名称：Human Epithelial Cell Adhesion Molecule,Ep-CAM ELISAkit 规格：48T/96Thz-E10038 中文名称：人可溶性粘附分子(Sam)ELISA试剂盒 英文名称：Human soluble adhesion molecules,Sam ELISAkit 规格：48T/96Thz-E10039 中文名称：人巨噬细胞替代激活相关化学因子1(AmAC-1)ELISA试剂盒 英文名称：Human Alternative macrophage activation-associated CC chemokine 1,AmAC-1 ELISAkit 规格：48T/96Thz-E10040 中文名称：人可溶性血管内皮生长因子受体2(VEGFR-2/sFLK-1)ELISA试剂盒 英文名称：Human soluble vascular endothelial growth factor receptor-2,VEGFR-2/sFLK-1 ELISAkit 规格：48T/96Thz-E10041 中文名称：人胸腺基质淋巴细胞生成素(TSLP)ELISA试剂盒 英文名称：Human thymic stromal lymphopoietin,TSLP ELISAkit 规格：48T/96Thz-E10042 中文名称：人穿孔素/成孔蛋白(PF/PFP)ELISA试剂盒 英文名称：Human Perfor in/Pore-for ming protein,PF/PFP ELISAkit 规格：48T/96Thz-E10043 中文名称：人多效生长因子(PTN)ELISA试剂盒 英文名称：Human pleiotrophin,PTN ELISAkit 规格：48T/96Thz-E10044 中文名称：人可溶性CD28(sCD28)ELISA试剂盒 英文名称：Human soluble cluster of differentiation 28,sCD28 ELISAkit 规格：48T/96Thz-E10045 中文名称：人淋巴细胞因子ELISA试剂盒 英文名称：Human lymphocyte factor ELISAkit 规格：48T/96Thz-E10046 中文名称：人胸腺活化调节趋化因子(TARC/CCL17)ELISA试剂盒 英文名称：Human thymus activation regulated chemokine,TARC ELISAkit 规格：48T/96Thz-E10047 中文名称：人神经细胞粘附分子配体1(NCAM-L1/CD171)ELISA试剂盒 英文名称：Human Neural cell adhesion molecule ligand 1,NCAM-L1 ELISAkit 规格：48T/96Thz-E10048 中文名称：人神经保护因子(CVNPF)ELISA试剂盒 英文名称：Human Cobra venom neuronal protective factor,CVNPF ELISAkit 规格：48T/96Thz-E10049 中文名称：人可溶性肿瘤坏死因子α受体(sTNFαR)ELISA试剂盒 英文名称：Human soluble Tumor Necrosis Factorαreceptor,sTNFαR ELISAkit 规格：48T/96Thz-E10050 中文名称：人可溶性细胞因子受体(sCKR)ELISA试剂盒 英文名称：Human soluble cytokine receptor,sCKR ELISAkit 规格：48T/96Thz-E10051 中文名称：人可溶性凋亡相关因子配体(sFASL)ELISA试剂盒 英文名称：Human soluble Factor-related Apoptosis ligand,sFASL/Apo-1 ELISAkit 规格：48T/96Thz-E10052 中文名称：人细胞凋亡抑制因子(IAP)ELISA试剂盒 英文名称：Human inhibitor of apoptosis,IAP ELISAkit 规格：48T/96Thz-E10053 中文名称：人集落刺激因子(CSF)ELISA试剂盒 英文名称：Human colony-stimulating factor,CSF ELISAkit 规格：48T/96Thz-E10054 中文名称：人γ干扰素诱导单核细胞因子(MIGF/CXCL9)ELISA试剂盒 英文名称：Human monocyte interferon gamma inducing factor,MIGF ELISAkit 规格：48T/96Thz-E10055 中文名称：人干扰素诱导T细胞趋化因子(ITAC/CXCL11)ELISA试剂盒 英文名称：Human Interferon inducible T-cell Chemoattractant,I-TAC ELISAkit 规格：48T/96Thz-E10056 中文名称：人CD14分子(CDl4)ELISA试剂盒 英文名称：Human cluster Of differentiation,CDl4 ELISAkit

在治疗肿瘤的过程中我们可能会使用被动注射肿瘤特异性抗体的方法。这种方法的原理是依靠抗体的中和效应将病原体进行识别，从而进一步被体内的巨噬细胞吞噬消灭，即"抗体依赖性的细胞毒性效应（ADCC）"。所以说，本质上这是一种短期的治疗方法。这一特点导致ADCC无法对肿瘤造成持续性的免疫效应。新的技术的出现帮我们寻找，鉴定肿瘤特异性的抗原，包括一些在肿瘤发生过程中新出现的抗原物质（neoantigens）。在最近的一项研究中，来自美国洛克菲勒大学的Jeffrey V. Ravetch与David J. DiLillo通过一种肿瘤特异性抗原的模型分析了被动注射抗体是否能够引起后续的免疫反应。首先，他们建立了一类特殊的小鼠肿瘤模型，该小鼠以B6为背景，随后向体内移植带有人特异性标签：hCD20（neoantigen）的小鼠EL-4淋巴癌细胞。之后，作者对这一小鼠进行被动的anti-hCD20的抗体注射治疗。结果显示，野生型小鼠在接受抗体治疗后能够从EL-4癌症中存活，而对照组（PBS注射）则快速死亡。类似地，缺乏FcR的小鼠即使在抗体注射治疗后也像对照组一样快速死亡。之后，作者将存活后的小鼠再次进行EL-4癌细胞的移植处理。结果显示，之前接受过hCD20抗体治疗的小鼠在二次刺激时同样能够存活，而对照组（一次刺激）则快速死亡；同时，作者发现在二次刺激时如果使用不带有hCD20标签的EL-4野生癌细胞，小鼠则会快速死亡，而如果使用带有hCD20标签的非EL-4癌细胞，小鼠依然存活。这些事实说明抗体的悲痛注射疗法具有长期的保护效应，而这种保护效应是依赖于抗体的识别特异性。而我们知道抗体在体内的半衰期通常较短，在以上事实中体现出的保护效应一定不是抗体本身的功劳，那么是什么原因导致抗体的注射会产生如此漫长的保护效应呢？作者怀疑免疫呈递在此过程中发挥了作用。因此他们比较了DC特异性Fc-gammRIV缺失突变体小鼠与野生型小鼠在以上处理后的表现。结果显示：DC缺失了Fc-gammRIV之后，抗体注射产生的长期保护效应不复存在。这一结果证实了作者的猜想。之后，作者变猜想免疫呈递后的T细胞激活可能参与了这一过程。因此，作者将上述试验中接受过刺激的小鼠体内的T细胞取出，并转移至另外一只没有经过抗体治疗的小鼠中，并随后进行癌细胞移植实验。结果显示：经过抗体治疗的小鼠T细胞能够对受体小鼠的癌症产生免疫保护效应，进一步的实验发现这一保护效应依赖于供体小鼠DC表面Fc-gammRIV的表达。因此作者认为抗体治疗之后小鼠体内产生了T细胞的肿瘤抗原特异性免疫激活效应。最后，作者利用人源化的小鼠验证了上述的实验结果，并发现在人体中Fc-gammaRIIA是关键的分子。hz-E10002 中文名称：人白介素2受体(IL-2R)ELISA试剂盒 英文名称：Human Interleukin-2 receptor,IL-2R ELISAkit 规格：48T/96Thz-E10003 中文名称：人皮肤T细胞虏获趋化因子(CTACK/CCL27)ELISA试剂盒 英文名称：Human cutaneous T cell-attracting chemokine,CTACK ELISAkit 规格：48T/96Thz-E10004 中文名称：人胸肾表达趋化因子(BRAK/CXCL14)ELISA试剂盒 英文名称：Human Breast and kidney expressed chemokine,BRAK ELISAkit 规格：48T/96Thz-E10005 中文名称：人B-淋巴细胞趋化因子1(BLC-1/CXCL13)ELISA试剂盒 英文名称：Human B-Lymphocyte Chemoattractant 1,BLC-1 ELISAkit 规格：48T/96Thz-E10006 中文名称：人结缔组织活化肽Ⅲ(CTAPⅢ)ELISA试剂盒 英文名称：Human connective tissue-activating peptide Ⅲ,CTAPⅢ ELISAkit 规格：48T/96Thz-E10007 中文名称：人免疫球蛋白A Fc段受体Ⅰ(FcαRⅠ/CD89)ELISA试剂盒 英文名称：Human Receptor Ⅰfor  the Fc region of immunoglobulin A,FcαRⅠ ELISAkit 规格：48T/96Thz-E10008 中文名称：人免疫球蛋白E Fc段受体Ⅱ(FcεRⅡ/CD23)ELISA试剂盒 英文名称：Human Receptor Ⅱ for  the Fc region of immunoglobulin E,FcεRⅡ ELISAkit 规格：48T/96Thz-E10009 中文名称：人免疫球蛋白G Fc段受体Ⅲ(FcγRⅢ/CD16)ELISA试剂盒 英文名称：Human Receptor Ⅲ for  the Fc region of immunoglobulin G,FcγRⅢ ELISAkit 规格：48T/96Thz-E10010 中文名称：人免疫球蛋白G Fc段受体Ⅱ(FcγRⅡ/CD32)ELISA试剂盒 英文名称：Human Receptor Ⅱ for  the Fc region of immunoglobulin G,FcγRⅡ ELISAkit 规格：48T/96Thz-E10011 中文名称：人免疫球蛋白G Fc段受体Ⅰ(FcγRⅠ/CD64)ELISA试剂盒 英文名称：Human ReceptorⅠfor  the Fc region of immunoglobulin G,FcγRⅠ ELISAkit 规格：48T/96Thz-E10012 中文名称：人粒细胞趋化蛋白-2(GCP-2/CXCL6)ELISA试剂盒 英文名称：Human granulocyte chemotactic protein-2,GCP-2 ELISAkit 规格：48T/96Thz-E10013 中文名称：人糖基化依赖的细胞黏附分子(GlyCAM-1)ELISA试剂盒 英文名称：Human glycosylation-dependent cell adhesion molecule-1,GlyCAM-1 ELISAkit 规格：48T/96Thz-E10014 中文名称：人干扰素调节因子(IRF)ELISA试剂盒 英文名称：Human interferon Regulatory Factor,IRF ELISAkit 规格：48T/96Thz-E10016 中文名称：人淋巴毒素α(LTA)ELISA试剂盒 英文名称：Human lymphotoxinα,LTA ELISAkit 规格：48T/96Thz-E10017 中文名称：人CC趋化因子受体1(CCR1)ELISA试剂盒 英文名称：Human CC-chemokine receptor 1,CCR1 ELISAkit 规格：48T/96Thz-E10018 中文名称：人CX3C趋化因子受体1(CX3CR1)ELISA试剂盒 英文名称：Human CX3C-chemokine receptor 1,CX3CR1 ELISAkit 规格：48T/96Thz-E10019 中文名称：人肺部活化调节趋化因子(PARC/CCL18)ELISA试剂盒 英文名称：Human pulmonary activation regulated chemokine,PARC ELISAkit 规格：48T/96Thz-E10020 中文名称：人黏膜地址素细胞黏附分子(MAdCAM-1)ELISA试剂盒 英文名称：Human mucosal addressin cell adhesion molecule-1,MAdCAM-1 ELISAkit 规格：48T/96Thz-E10021 中文名称：人β干扰素(IFN-β/IFNB)ELISA试剂盒 英文名称：Human Interferon β,IFN-β/IFNB ELISAkit 规格：48T/96Thz-E10022 中文名称：人可溶性CD38(sCD38)ELISA试剂盒 英文名称：Human Soluble Cluster of differentiation 38,sCD38 ELISAkit 规格：48T/96Thz-E10023 中文名称：人可溶性CD21(CR2/sCD21)ELISA试剂盒 英文名称：Human Soluble Cluster of differentiation 21,sCD21 ELISAkit 规格：48T/96Thz-E10024 中文名称：人可溶性瘦素受体(sLR)ELISA试剂盒 英文名称：Human Leptin Soluble Receptor,sLR ELISAkit 规格：48T/96Thz-E10025 中文名称：人Toll样受体9(TLR-9/CD289)ELISA试剂盒 英文名称：Human Toll-like receptor 9,TLR-9 ELISAkit 规格：48T/96Thz-E10026 中文名称：人转化生长因子β2(TGFβ2)ELISA试剂盒 英文名称：Human transfor ming growth factors β2,TGFβ2 ELISAkit 规格：48T/96Thz-E10027 中文名称：人单核细胞趋化蛋白4(MCP-4/CCL13)ELISA试剂盒 英文名称：Human monocyte chemotactic protein 4,MCP-4 ELISAkit 规格：48T/96Thz-E10028 中文名称：人白三烯D4(LTD4)ELISA试剂盒 英文名称：Human leukotriene D4,LT-D4 ELISAkit 规格：48T/96T

 人P选择素(P-Selectin/CD62P)ELISA Kit产品类型:ELISA Kit产品名称:人P选择素(P-Selectin/CD62P)ELISA Kit英文名称:Human P-Selectin ELISA kit货号:人P选择素(P-Selectin/CD62P)ELISA Kit规格:96T中文价格:人P选择素(P-Selectin/CD62P)ELISA Kit种属:Human待测物名称:P-Selectin缩写:P-Selectin/CD62P检测范围:0.9 ng/ml-60 ng/ml灵敏度:0.225 ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for p-selectin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any p-selectin present is bound by the immobilized antibody. After removing any unbound substances, 人P选择素(P-Selectin/CD62P)ELISA Kit   a biotin-conjugated antibody specific for p-selectin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of p-selectin bound in the initial step. The color development is stopped and the intensity of the color is measured.

大鼠前白蛋白(PA)ELISA Kit产品类型:ELISA Kit产品名称:大鼠前白蛋白(PA)ELISA Kit英文名称:Rat prealbumin,PA ELISA Kit货号:大鼠前白蛋白(PA)ELISA Kit别名:N/A规格:96T中文价格:大鼠前白蛋白(PA)ELISA Kit种属:Rat其他种属:Dog待测物名称:prealbumin,PA缩写:PA检测范围:375 ng/ml-6000 ng/ml灵敏度:375 ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PA. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for PA. 大鼠前白蛋白(PA)ELISA Kit   The competitive inhibition reaction is launched between with pre-coated PA and PA in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of PA in the samples. The color development is stopped and the intensity of the color is measured.

前列腺癌为何骨转移？一关键信号途径在作祟抑制TGF-β信号途径能够降低促癌转移基因表达和前列腺癌骨转移 PMEPA1通过非蛋白酶体机制抑制TGF-β信号途径 在临床上，未发生癌转移的前列腺癌病人生存率较低也与PMEPA1低表达有关 抑制PMEPA1表达会增加前列腺癌小鼠模型发生骨转移  近日，著名国际学术期刊cancer cell在线发表了美国科学家的一项最新研究进展，他们发现一个能够抑制TGF-β信号途径的基因对于抑制前列腺癌骨转移具有重要作用。 TGF-β能够调节促进乳腺癌骨转移的相关基因的表达，但TGF-β与前列腺癌骨转移之间存在什么样的关系仍没有研究。在该项研究中，研究人员发现TGFBR1抑制剂SD208能够有效抑制前列腺癌骨转移。研究人员利用前列腺癌细胞进行研究发现，TGF-β能够上调一系列与前列腺癌侵袭性和骨转移相关的基因，其中上调最明显的是一个叫做PMEPA1的基因。随后，研究人员对前列腺癌病人的样本进行了研究分析，发现PMEPA1在转移性前列腺癌中发生表达下降，并且未发生转移的前列腺癌病人生存率下降也与PMEPA1低表达有关。随后，研究人员对其中的机制进行了研究，结果发现只有具有膜锚定能力的PMEPA1亚型能够与R-SMAD和泛素连接酶发生相互作用，阻断TGF-β信号途径，但这一过程并不依赖于蛋白酶体。最后，研究人员在前列腺癌小鼠模型中敲低PMEPA1表达，结果发现阻断这一负反馈途径能够增加促癌转移基因表达，并促进前列腺癌骨转移。 综上所述，这项研究发现TGF-β负调控因子PMEPA1能够通过抑制TGF-β信号途径抑制前列腺癌发生骨转移，并且这种抑制作用是通过不依赖蛋白酶体的泛素化途径实现的，这对于了解TGF-β与前列腺癌转移之间的关系具有重要意义

 人转录因子AP-1 ELISA kit产品类型:ELISA Kit产品名称:人转录因子AP-1 ELISA kit英文名称:Human Transcription Factor/Activator Protein 1 (AP-1) ELISA kit人转录因子AP-1 ELISA kit别名:AP-1, AP1, c-Jun, Jun activation domain binding protein|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog规格:96T人转录因子AP-1 ELISA kit种属:Human待测物名称:jun oncogene缩写:JUN蛋白功能1:Transcription/Transcription regulation蛋白功能3:Transcription检测范围:47 pg/ml-3000 pg/ml灵敏度:11.75 pg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AP-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

人亮氨酸氨基肽酶3(LAP3)ELISA Kit产品类型:ELISA Kit产品名称:人亮氨酸氨基肽酶3(LAP3)ELISA Kit英文名称:Human leucine aminopeptidase 3(LAP3) ELISA Kit人亮氨酸氨基肽酶3(LAP3)ELISA Kit别名:LAP, LAPEP, PEPS, peptidase S, Leucyl aminopeptidase , leucine aminopeptidase 3 (LAP3)规格:96T人亮氨酸氨基肽酶3(LAP3)ELISA Kit种属:Human待测物名称:leucine aminopeptidase 3(LAP3)缩写:LAP3检测范围:3.12 mU/ml-200 mU/ml灵敏度:0.78 mU/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LAP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LAP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

近日，来自德克萨斯大学MD癌症研究中心的研究人员通过研究发现，阻断一种名为FGL2的促癌蛋白的功能或许就可以帮助开发治疗大脑癌症的新型疗法，相关研究刊登于国家杂志JNCI Journal of the National Cancer Institute上。纤维蛋白原样蛋白（FGL2）是一种在脑部肿瘤表达的常见蛋白，其会通过多种途径抑制机体免疫系统从而促进癌症发生，研究者发现FGL2可以通过调节蛋白PD1和CD39来促进大脑癌症的发生。研究者Shulin Li教授表示，我们都知道癌症会通过开发一系列的编辑机制来躲避免疫检测和清除，从而间接躲避免疫监督，而其中一种机制就是拦截免疫细胞的检查点，推翻免疫系统并且允许肿瘤生长。FGL2可以调节免疫系统中名为检查点的“闸口”，同时也会调节免疫抑制细胞，后者可以阻断机体免疫系统对癌细胞的天然攻击；研究者发现，FGL2可以扮演一种关键的开关来抑制肿瘤免于被免疫系统检测，同时FGL2还会通过增强免疫检查点的基因表达来增加小鼠机体中肿瘤的生长，研究小组利用抗FGL2的抗体就可以中和FGL2蛋白的作用。相比接受对照抗体的小鼠而言，接受FGL2抗体治疗的小鼠的平均存货时间要明显延长很多，更有意思的是，17只进行FGL2抗体治疗的小鼠中有4只小鼠机体中竟然完全没有了肿瘤组织。研究者还发现FGL2的表达和肿瘤的侵略性之间存在一定的关联，机体中FGL2高水平表达的患者相比低水平表达的患者往往死亡率较低。最后研究者表示，本文研究中我们阐明了FGL2在免疫抑制过程中的角色，对于后期理解关键的信号通路，乃至开发出抗FGL2的新型疗法，从而有效抑制大脑肿瘤恶化发展提供了新的线索和思路。人5脂加氧酶(5-LO)检测试剂盒 人5核苷酸酶(5-NT)检测试剂盒  人6酮前列腺素(6-K-PG)检测试剂盒 人6-羟多巴胺(6-OHDA)检测试剂盒  人抗肌动蛋白抗体(AAA)检测试剂盒 人5羟基二十烷四烯酸(5-HETE)检测试剂盒 人抗白蛋白抗体(AAA)检测试剂盒  人α1抗胰糜蛋白酶(α1ACT)检测试剂盒 人白介素16(IL-16)检测试剂盒人Apelin 17蛋白(AP17)检测试剂盒  人双调蛋白(AR)检测试剂盒 人抑制素A(INHA)检测试剂盒人芳香酶(ARO)检测试剂盒人胆绿素还原酶B(BLVRB)检测试剂盒人抗心磷脂抗体IgA(ACA-IgA)检测试剂盒 人抗心磷脂抗体IgG(ACA-IgG)检测试剂盒 人抗心磷脂抗体IgM(ACA-IgM)检测试剂盒 人肾上腺皮质发育异常蛋白(ACD)检测试剂盒 人乙酰胆碱(ACH)检测试剂盒 人乙酰胆碱酯酶(AChE)检测试剂盒  人乙酰胆碱受体抗体(AChRab)检测试剂盒 人乌头酸酶2(ACO-2)检测试剂盒  人白介素18(IL-18)检测试剂盒人白介素19(IL-19)检测试剂盒人γ1肌动蛋白(ACTG1)检测试剂盒人辅肌动蛋白α2 (ACTN2)检测试剂盒人辅肌动蛋白α3(ACTN3)检测试剂盒人平滑肌肌动蛋白α2(ACTα2)检测试剂盒人骨骼肌肌动蛋白α1(ACTα1)检测试剂盒 人腺苷脱氨酶(ADA)检测试剂盒  人解整合素金属蛋白酶10(ADAM10)检测试剂盒  人解整合素金属蛋白酶8(ADAM8)检测试剂盒 人解整合素金属蛋白酶9(ADAM9)检测试剂盒 人ADAM金属肽酶含血小板反应蛋白1基元4(ADAMTS4)检测试剂盒 人腺苷酸环化酶1(ADCY1)检测试剂盒 人乙醇脱氢酶1(ADH1)检测试剂盒 人抗利尿激素(ADH)检测试剂盒人脂联素受体1(ADIPOR1)检测试剂盒 人脂联素受体2(ADIPOR2)检测试剂盒 人肾上腺髓质素(ADM)检测试剂盒  人肾上腺素能α1A受体(ADRA1A)检测试剂盒人脂肪分化相关蛋白(ADRP)检测试剂盒 人白介素20(IL-20)检测试剂盒人δ样蛋白1同源物(DLK-1)检测试剂盒人血管紧张素转化酶2(ACE2)检测试剂盒 人骨成型蛋白3(BMP-3)检测试剂盒 人钙磷蛋白(CAPS)检测试剂盒人脂肪细胞型脂肪酸结合蛋白(FABP4)检测试剂盒 人甲胎蛋白异质体3(AFP-L3)检测试剂盒 人αL岩藻糖苷酶(FUCA)检测试剂盒 人细胞周期素B(CCNB)检测试剂盒 

根据美国癌症学会的估计，2015年美国将有220,800例新增前列腺癌病例，并切有大约27,540名病人将死于前列腺癌，这占到了所有癌症死亡总数的5%。 治疗前列腺癌的一种常见手段是前列腺切除，对前列腺进行全部或部分切除。但早在2010年，一篇发表在新英格兰医学杂志上的文章就曾指出前列腺切除术的治疗效果并不理想，48名进行该手术的病人只有1人生命得到了延长。并且该手术会带来一些副作用，包括尿失禁和阳痿，大大影响了病人的生活质量。 一位研究人员指出，每20名进行前列腺切除术的病人大约只有一人能够继续生存下去，而其他的19名病人就没有那么幸运了，其生活质量甚至不如术前，切除前列腺带来的副作用大大影响了病人的生活质量。 在最近一项发表在国际学术期刊scientific reports上的文章中，研究人员开发出一种空间光干涉显微镜，通过这种无标记的方法，能够对前列腺组织中具有光散射的部位进行定位测量。利用这种显微镜进行定量相位成像，能够对材料的物理特性差异和非均质性进行检测。研究人员发现非均质性测量数值越高，就说明组织内部排列更有条理，而这一数值越低，就说明组织存在碎片化，排列杂乱无章。 利用该方法，研究人员能够在术前或活检阶段就对癌变腺体周围组织进行检测，从而确定前列腺癌的发展阶段，对进行前列腺癌切除术后是否会出现复发进行准确预测。人铜蓝蛋白(CP)检测试剂盒人α-骨胶原交联(α-CTx)检测试剂盒人白细胞分化抗原CD42 (CD42)检测试剂盒人白细胞抗原B27(HLA-B27)检测试剂盒人成纤维细胞生长因子受体2(FGFR2)检测试剂盒 人肠脂肪酸结合蛋白(IFABP)检测试剂盒人脂蛋白a(LP-a)检测试剂盒人髓鞘碱性蛋白(MBP)检测试剂盒人端粒酶(TE)检测试剂盒人组织因子途径抑制因子(TFPI)检测试剂盒人血栓调节蛋白(TM)检测试剂盒人补体因子4 (C4)检测试剂盒人铁蛋白(FE)检测试剂盒人免疫球蛋白G(IgG)检测试剂盒人血清转铁蛋白(TF)检测试剂盒人卵泡抑素样蛋白1(FSTL1)检测试剂盒人赖氨酰氧化酶(LOX)检测试剂盒 人绒毛膜促性腺激素(CG)检测试剂盒人生长激素(GH)检测试剂盒人IV型胶原(COL4)检测试剂盒人纤维连接蛋白(FN)检测试剂盒人白介素9(IL-9)检测试剂盒人I型前胶原(PCI)检测试剂盒人III型前胶原(PCIII)检测试剂盒人III型前胶原氨基端原肽(PIIINP)检测试剂盒人基质金属蛋白酶组织抑制因子1(TIMP-1)检测试剂盒人I型前胶原氨基端原肽(PINP)检测试剂盒人肾损伤分子1(KIM-1)检测试剂盒人腺苷酸环化酶2(ADCY2)检测试剂盒 人G补缀FHA域血管生成因子1(AGGF1)检测试剂盒人补体成分4a(C4a)检测试剂盒人补体成分5a(C5a)检测试剂盒人白介素5(IL-5)检测试剂盒人白介素6受体(IL-6R)检测试剂盒人免疫球蛋白G Fc段受体I(FcγR I)检测试剂盒 人神经钙黏蛋白(NCAD)检测试剂盒 人I型前胶原羧基端原肽(PICP)检测试剂盒人血小板衍生生长因子受体样蛋白(PDGFRL)检测试剂盒人基膜聚糖(LUM)检测试剂盒人泪腺富脯氨酸蛋白4(PRR4)检测试剂盒人鞘脂激活蛋白原(PSAP)检测试剂盒人前列腺素E合成酶(PTGES)检测试剂盒人干扰素刺激基因15(ISG15)检测试剂盒人氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)检测试剂盒人11去氢血栓烷B2(11-DH-TXB2)检测试剂盒 人12羟基二十烷四烯酸(12-HETE)检测试剂盒 人15脂加氧酶(15-LO)检测试剂盒 人16α羟基雌酮1(16-α OHE-1)检测试剂盒 人β位淀粉样前体蛋白裂解酶1(BACE1)检测试剂盒人白介素32(IL-32)检测试剂盒人肿瘤坏死因子受体超家族成员1A(TNFRSF1A)检测试剂盒人26S蛋白酶体(26S-PSM)检测试剂盒  人5羟基吲哚乙酸(5-HIAA)检测试剂盒 人白介素15(IL-15)检测试剂盒

牛叶酸(FA)ELISA Kit产品类型:ELISA Kit产品名称:牛叶酸(FA)ELISA Kit英文名称:Bovine folic acid,FA ELISA Kit货号:HZ-E13074B别名:N/A规格:96T种属:Bovine其他种属:Dog Human Mouse Rat Goat待测物名称:folic acid,FA缩写:FA蛋白功能1:Metabolism检测范围:7.8 pg/ml-500 pg/ml灵敏度:1.95 pg/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nm

 小鼠凝血因子Ⅷ（FⅧ）ELISA Kit产品类型:ELISA Kit产品名称:小鼠凝血因子Ⅷ（FⅧ）ELISA Kit英文名称:Mouse coagulation factor Ⅷ(FⅧ) ELISA Kit货号:HZ-E13084m别名:RP11-115M6.7, AHF, DXS1253E, F8B, F8C, FVIII, HEMA, coagulation factor VIII|coagulation factor VIIIc|factor VIII F8B规格:96T种属:Mouse待测物名称:coagulation factor VIII, procoagulant component缩写:F8蛋白功能1:Immunity蛋白功能3:Acute phase检测范围:0.312 ng/ml-20 ng/ml灵敏度:0.078 ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FⅧ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FⅧ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FⅧ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FⅧ bound in the initial step. The color development is stopped and the intensity of the color is measured.

猪凝血因子Ⅱ(FⅡ)ELISA Kit产品类型:ELISA Kit产品名称:猪凝血因子Ⅱ(FⅡ)ELISA Kit英文名称:Pig coagulation factor Ⅱ,FⅡ ELISA Kit货号:HZ-E14999p别名:PT, coagulation factor II|prothrombin B-chain|serine protease规格:96T种属:Pig待测物名称:coagulation factor II (thrombin)缩写:F2蛋白功能1:Immunity蛋白功能3:Acute phase检测范围:3.9 ng/ml-1000 ng/ml灵敏度:3.9 ng/ml反应时间:1-5h所需样本体积:50-100ul检测波长:450 nmThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with pig FⅡ. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for pig FⅡ. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of pig FⅡ in samples. The color development is stopped and the intensity of the color is measured.

淀粉样蛋白β1-42(Aβ1-42)检测试剂盒适用生物     Homo sapiens (Human，人)    淀粉样蛋白β1-42(Aβ1-42)检测试剂盒    检测范围     12.35-1000pg/mL     灵敏度     4.53pg/mL    样本类型     Serum, plasma and other biological fluids.    实验时长     2.5h     实验方法     竞争抑制法     淀粉样蛋白β1-42(Aβ1-42)检测试剂盒规格     96T    ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    淀粉样蛋白β1-42(Aβ1-42)检测试剂盒Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     2.5 hours    Detection range     12.35-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 333.33pg/mL, 111.11pg/mL, 37.04pg/mL, 12.35pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 4.53pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Amyloid Beta Peptide 1-42 (Ab1-42). No significant cross-reactivity or interference between Amyloid Beta Peptide 1-42 (Ab1-42) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Amyloid Beta Peptide 1-42 (Ab1-42) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Beta Peptide 1-42 (Ab1-42) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     98-105     102    EDTA plasma(n=5)     97-105     102    heparin plasma(n=5)     96-105     101    PrecisionIntra-assay Precision 淀粉样蛋白β1-42(Aβ1-42)检测试剂盒(Precision within an assay): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Beta Peptide 1-42 (Ab1-42) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Beta Peptide 1-42 (Ab1-42) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     84-94%     86-99%     90-97%     79-94%    EDTA plasma(n=5)     86-98%     78-88%     78-102%     86-101%    heparin plasma(n=5)     88-95%     86-95%     80-102%     84-95%    StabilityThe stability of kit is淀粉样蛋白β1-42(Aβ1-42)检测试剂盒 determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    Reagent Diluent     1×300μL     Stop Solution     1×6mL    TMB Substrate     1×9mL     Instruction manual     1    Wash Buffer (30 × concentrate)     1×20mL    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 50μL standard or sample to each well.    And then add 50μL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37oC;3. Aspirate and wash 3 times;4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;5. Aspirate and wash 5 times;6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;7. Add 50μL Stop Solution. Read at 450 nm immediately.Test principleThis assay employs the淀粉样蛋白β1-42(Aβ1-42)检测试剂盒 competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Amyloid Beta Peptide 1-42 (Ab1-42) and unlabeled Amyloid Beta Peptide 1-42 (Ab1-42) (Standards or samples) with the pre-coated antibody specific to Amyloid Beta Peptide 1-42 (Ab1-42). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Amyloid Beta Peptide 1-42 (Ab1-42) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Amyloid Beta 淀粉样蛋白β1-42(Aβ1-42)检测试剂盒Peptide 1-42 (Ab1-42) in the sample.Gene/ItemProteinsAntibodiesAssay Kits        Package and ComponentsReagent PreparationResults demonstrationTypical Standard CurveCertificateDownloadFree use and GuaranteesFree trialIn case it is difficult to pre-evaluate experiment result because of specific test samples or other reasons, free trial can be offered.If the budget is really limited or other special situation, you can apply the free products.GuaranteesUnconditionally return policy within 72 hours.For any complaint, 淀粉样蛋白β1-42(Aβ1-42)检测试剂盒we promise to reply within one work day, resolve the problem within three work days. Implementing initial consultation system in order to provide satisfactory service for all the customers. Customers can unconditionally to request a charge back or refund.Benchmark pricePlease consult local dealer

葡萄糖激酶(GCK)检测试剂盒适用生物     Homo sapiens (Human，人)    葡萄糖激酶(GCK)检测试剂盒    检测范围     0.781-50ng/mL     灵敏度     0.23ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法     葡萄糖激酶(GCK)检测试剂盒规格     96T    ELISA Kit for Glucokinase (GCK)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    葡萄糖激酶(GCK)检测试剂盒   Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.781-50ng/mL The standard curve concentrations used for the ELISA’s were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL, 0.781ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.23ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Glucokinase (GCK). No significant cross-reactivity or interference between Glucokinase (GCK) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glucokinase (GCK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glucokinase (GCK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by 葡萄糖激酶(GCK)检测试剂盒the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL 葡萄糖激酶(GCK)检测试剂盒prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in葡萄糖激酶(GCK)检测试剂盒 this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucokinase (GCK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucokinase (GCK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucokinase (GCK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is 葡萄糖激酶(GCK)检测试剂盒measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glucokinase (GCK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒适用生物     Homo sapiens (Human，人)    Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒   检测范围     123.46-10000pg/mL     灵敏度     44.3pg/mL    样本类型     Serum, plasma and other biological fluids.    实验时长     2.5h     实验方法     竞争抑制法    Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒规格     96T    ELISA Kit for Cross Linked C-Telopeptide Of Type I Collagen (CTXI)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     2.5 hours    Detection range     123.46-10000pg/mL The standard curve concentrations used for the ELISA’s were 10000pg/mL, 3333.33pg/mL, 1111.11pg/mL, 370.37pg/mL, 123.46pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 44.3pg/mL.    SpecificityThis assay has high Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒sensitivity and excellent specificity for detection of Cross Linked C-Telopeptide Of Type I Collagen (CTXI). No significant cross-reactivity or interference between Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and the recovery rates were calculated by comparing the measured value to the expected amount of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     98-105     101    EDTA plasma(n=5)     78-97     82    heparin plasma(n=5)     90-97     94    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Type I Collagen (CTXI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cross Linked C-Telopeptide Of Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒Type I Collagen (CTXI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     95-102%     93-103%     87-97%     96-105%    EDTA plasma(n=5)     99-105%     84-95%     88-96%     81-103%    heparin plasma(n=5)     98-105%     79-90%     87-95%     79-94%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    Reagent Diluent     1×300μL     Stop Solution     1×6mL    TMB Substrate     1×9mL     Instruction manual     1    Wash Buffer (30 × concentrate)     1×20mL    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 50μL standard or sample to each well.    And then add 50μL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37oC;3. Aspirate and wash 3 times;4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;5. Aspirate and wash 5 times;6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;7. Add 50μL Stop Solution. Read at 450 nm immediately.Test principleThis assay employs theⅠ型胶原交联羧基端肽(CTXⅠ)检测试剂盒 competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Cross Linked C-Telopeptide Of Type I Collagen (CTXI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Cross Linked C-Telopeptide Of Type I Collagen (CTXI) and unlabeled Cross Linked C-Telopeptide Of Type I Collagen (CTXI) (Standards or samples) with the pre-coated antibody specific to Cross Linked C-Telopeptide Of Type I Collagen (CTXI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Type I Collagen (CTXI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Cross Linked C-Telopeptide Of Ⅰ型胶原交联羧基端肽(CTXⅠ)检测试剂盒Type I Collagen (CTXI) in the sample.

白介素1受体关联激酶3(IRAK3)检测试剂盒适用生物     Homo sapiens (Human，人)    白介素1受体关联激酶3(IRAK3)检测试剂盒   检测范围     0.781-50ng/mL     灵敏度     0.32ng/mL    样本类型     Serum, plasma, tissue homogenates, cell lysates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法    白介素1受体关联激酶3(IRAK3)检测试剂盒规格     96T    ELISA Kit for Interleukin 1 Receptor Associated Kinase 3 (IRAK3)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    白介素1受体关联激酶3(IRAK3)检测试剂盒  Sample type     Serum, plasma, tissue homogenates, cell lysates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.781-50ng/mL The standard curve concentrations used for the ELISA’s were 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.125ng/mL, 1.563ng/mL, 0.781ng/mL    Sensitivity     The minimum detectable 白介素1受体关联激酶3(IRAK3)检测试剂盒dose of this kit is typically less than 0.32ng/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 3 (IRAK3). No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 3 (IRAK3) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Interleukin 1 Receptor Associated Kinase 3 (IRAK3) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 1 Receptor Associated Kinase 3 (IRAK3) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     79-93     87    EDTA plasma(n=5)     82-98     86    heparin plasma(n=5)     81-98     90    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated白介素1受体关联激酶3(IRAK3)检测试剂盒 Kinase 3 (IRAK3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 3 (IRAK3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 1 Receptor Associated 白介素1受体关联激酶3(IRAK3)检测试剂盒Kinase 3 (IRAK3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     83-95%     87-101%     88-102%     89-98%    EDTA plasma(n=5)     89-97%     83-95%     78-96%     85-97%    heparin plasma(n=5)     97-105%     93-101%     99-105%     94-103%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that白介素1受体关联激酶3(IRAK3)检测试剂盒 the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this kit is白介素1受体关联激酶3(IRAK3)检测试剂盒 Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 3 (IRAK3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 3 (IRAK3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 3 (IRAK3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 3 (IRAK3) in the samples is白介素1受体关联激酶3(IRAK3)检测试剂盒 then determined by comparing the O.D. of the samples to the standard curve.

白介素1受体关联激酶4(IRAK4)检测试剂盒适用生物     Homo sapiens (Human，人)    白介素1受体关联激酶4(IRAK4)检测试剂盒   检测范围     1.56-100ng/mL     灵敏度     0.54ng/mL    样本类型     Tissue homogenates and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法    白介素1受体关联激酶4(IRAK4)检测试剂盒规格     96T    ELISA Kit for Interleukin 1 Receptor Associated Kinase 4 (IRAK4)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    白介素1受体关联激酶4(IRAK4)检测试剂盒  Sample type     Tissue homogenates and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     1.56-100ng/mL The standard curve concentrations used for the ELISA’s were 100ng/mL, 50ng/mL, 25ng/mL, 12.5ng/mL, 6.25ng/mL, 3.13ng/mL, 1.56ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.54ng/mL.    SpecificityThis assay has high sensitivity and 白介素1受体关联激酶4(IRAK4)检测试剂盒excellent specificity for detection of Interleukin 1 Receptor Associated Kinase 4 (IRAK4). No significant cross-reactivity or interference between Interleukin 1 Receptor Associated Kinase 4 (IRAK4) and analogues was observed.PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 1 Receptor Associated 白介素1受体关联激酶4(IRAK4)检测试剂盒Kinase 4 (IRAK4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 1 Receptor Associated Kinase 4 (IRAK4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVStabilityThe stability of kit is determined by 白介素1受体关联激酶4(IRAK4)检测试剂盒the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that白介素1受体关联激酶4(IRAK4)检测试剂盒 the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or 白介素1受体关联激酶4(IRAK4)检测试剂盒sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied in this白介素1受体关联激酶4(IRAK4)检测试剂盒 kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 4 (IRAK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. 白介素1受体关联激酶4(IRAK4)检测试剂盒The concentration of Interleukin 1 Receptor Associated Kinase 4 (IRAK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

颗粒溶素(GNLY)检测试剂盒适用生物     Homo sapiens (Human，人)    颗粒溶素(GNLY)检测试剂盒   检测范围     0.234-15ng/mL     灵敏度     0.09ng/mL    样本类型     Serum, plasma and other biological fluids.    实验时长     4.5h     实验方法     双抗夹心法   颗粒溶素(GNLY)检测试剂盒规格     96T    ELISA Kit for Granulysin (GNLY)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Homo sapiens (Human)    颗粒溶素(GNLY)检测试剂盒   Sample type     Serum, plasma and other biological fluids.    Format     96-well strip plate    Assay length     4.5 hours    Detection range     0.234-15ng/mL The standard curve concentrations used for the ELISA’s were 15ng/mL, 7.5ng/mL, 3.75ng/mL, 1.875ng/mL, 0.938ng/mL, 0.469ng/mL, 0.234ng/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 0.09ng/mL.    SpecificityThis assay has high sensitivity and颗粒溶素(GNLY)检测试剂盒 excellent specificity for detection of Granulysin (GNLY). No significant cross-reactivity or interference between Granulysin (GNLY) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Granulysin (GNLY) and the recovery rates were calculated by comparing the measured value to the expected amount of Granulysin (GNLY) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     90-99     95    EDTA plasma(n=5)     94-103     98    heparin plasma(n=5)     78-88     80    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granulysin (GNLY) were 颗粒溶素(GNLY)检测试剂盒tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granulysin (GNLY) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Granulysin (GNLY) and their 颗粒溶素(GNLY)检测试剂盒serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     96-103%     95-105%     92-101%     84-92%    EDTA plasma(n=5)     98-105%     92-105%     93-101%     93-101%    heparin plasma(n=5)     80-94%     97-105%     81-98%     96-104%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that颗粒溶素(GNLY)检测试剂盒 the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1×120μL     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    TMB Substrate     1×9mL     Stop Solution     1×6mL    Wash Buffer (30 × concentrate)     1×20mL     Instruction manual     1    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 100μL standard or sample to each well. Incubate 2 hours at 37oC;3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37oC;4. Aspirate and wash 3 times;5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;6. Aspirate and wash 5 times;7. Add 90μL Substrate Solution颗粒溶素(GNLY)检测试剂盒. Incubate 15-25 minutes at 37oC;8. Add 50μL Stop Solution. Read at 450nm immediately. Test principleThe test principle applied颗粒溶素(GNLY)检测试剂盒 in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Granulysin (GNLY). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Granulysin (GNLY). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Granulysin (GNLY), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Granulysin (GNLY) in 颗粒溶素(GNLY)检测试剂盒the samples is then determined by comparing the O.D. of the samples to the standard curve.

近日，丹麦科学家Claus Storgaard S?rensen在国际学术期刊nature reviews drug discovery发表了一篇综述文章，对利用癌细胞DNA复制应激进行癌症治疗的相关研究进展进行了总结讨论和展望。 癌细胞的DNA复制一般会伴随着复制叉的拖延和崩溃，以及应答DNA损伤和未成熟有丝分裂的信号通路激活，这些过程统称为"复制应激"。目前有许多研究对复制应激过程进行了大量探讨，并在这方面取得了显著进展，增加了人们对于调节复制应激过程的分子机制的理解，同时，也为通过增强复制应激开发癌症治疗手段提供了大量机会。除了拖延癌细胞周期进展，癌症治疗还可以进一步促进癌细胞通过周期检验点，增加复制应激，最终诱导细胞增殖的毁灭性失败，从而达到杀伤癌细胞治疗癌症的目的。 在这篇综述文章中，作者对目前的相关研究进展进行了总结。首先对DNA复制应激以及肿瘤细胞内的复制应激过程进行了系统性介绍，随后作者对可增强复制应激的药物以及其他可增强复制应激的方式进行了总结，提出将药物进行联合使用或与传统的化疗方法结合可进一步促进DNA复制应激，并且在一些病例中已经证明药物联合使用具有协同作用。但利用DNA复制应激进行癌症治疗也存在一些风险，作者在文章中针对应用该策略治疗癌症的风险以及如何保护正常细胞进行了总结和讨论。 最后，作者对未来利用DNA复制应激治疗癌症如何选取治疗靶点进行了探讨和展望。 综上所述，该文章对利用DNA复制应激进行癌症治疗的相关研究进展进行了总结，作者提出了许多新的问题和想法，并对未来如何进行DNA复制应激机制研究以及选取治疗靶点进行了探讨和展望，对于开发新的癌症治疗策略具有重要指导意义。人生长分化因子15(GDF15)检测试剂盒人粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒 人可溶性糖蛋白130(sgp130)检测试剂盒人肝细胞生长因子(HGF)检测试剂盒 人β干扰素(IFN-β)检测试剂盒人胰岛素样生长因子1(IGF-1)检测试剂盒 人胰岛素样生长因子结合蛋白3(IGFBP-3)检测试剂盒人白介素1α(IL-1α)检测试剂盒人白介素1受体拮抗剂(IL-1ra)检测试剂盒人白介素1受体II(IL-1R2)检测试剂盒人前列腺特异性抗原(PSA/KLK3)检测试剂盒人成纤维细胞生长因子7(FGF7)检测试剂盒人瘦素受体(LEPR)检测试剂盒人白血病抑制因子(LIF)检测试剂盒人肿瘤坏死因子配体超家族成员14(TNFSF14)检测试剂盒人中性粒细胞明胶酶相关脂质运载蛋白(NGAL)检测试剂盒人巨噬细胞集落刺激因子(MCSF)检测试剂盒人基质金属蛋白酶原1(Pro-MMP-1)检测试剂盒人白介素2(IL-2)检测试剂盒人白介素3(IL-3)检测试剂盒人白介素4(IL-4)检测试剂盒人白介素6(IL-6)检测试剂盒人白介素10(IL-10)检测试剂盒人白介素13(IL-13)检测试剂盒人白介素17(IL-17)检测试剂盒人白介素22(IL-22)检测试剂盒人白介素23(IL-23)检测试剂盒人γ干扰素(IFN-γ)检测试剂盒人肿瘤坏死因子α(TNF-α)检测试剂盒人转化生长因子β1(TGF-β1)检测试剂盒 人血管内皮细胞生长因子(VEGF-A)检测试剂盒 人表皮调节素(EREG)检测试剂盒人瘦素(LEP)检测试剂盒人B细胞淋巴瘤因子2(Bcl-2)检测试剂盒

近日，来自美国NIH的科学家在国际学术期刊journal of hepatology在线发表了一项最新研究进展，他们发现利用姜黄素对肝癌细胞系进行处理可以特异性抑制癌症干细胞生长，并且对NF-kB和HDAC两条信号途径进行联合抑制可能是治疗具有不良预后的肝癌病人的有效策略。 癌症干细胞对包括肝细胞癌在内的多种癌症产生治疗抵抗具有重要促进作用。而在肝脏癌症干细胞中，NF-kB信号途径常发生突变。 在该项研究中，研究人员利用IKK 抑制因子姜黄素对不同肝细胞癌细胞系进行处理，表现出不同应答特征，根据敏感性不同可分为敏感性细胞系和抵抗性细胞系。在敏感性细胞系中，姜黄素介导的细胞死亡与NF-kB受抑制程度直接相关，并且姜黄素处理还会导致侧群细胞减少，克隆球形成能力下降，癌症干细胞标记表达下降以及肿瘤形成受到抑制，这都表明姜黄素可以导致癌症干细胞出现特异性缺失。与此类似，利用特异性多肽SN50抑制NF-kB或用针对p65的siRNA进行抑制均会抑制肿瘤细胞生长。而在抵抗性细胞系中，用姜黄素进行处理会促进细胞增殖，上调癌症干细胞标记的表达。 随后研究人员对敏感性细胞系中姜黄素对癌症干细胞的特异性抑制机制进行了研究，结果发现姜黄素的抑制作用与NF-kB介导的HDAC抑制有关，用I/II类HDAC抑制剂trichostatine处理抵抗性细胞会促进此类细胞对姜黄素的敏感性。 综上所述，这项研究表明阻断NF-kB可以特异性靶向癌症干细胞，并且NF-kB和HDAC两条途径的联合抑制对于治疗具有不良预后的肝癌病人可能具有潜在效果。人髓样前体细胞抑制因子2(MPIF2)检测试剂盒人巨噬细胞炎性蛋白4α(MIP4α)检测试剂盒人皮肤T细胞虏获趋化因子(CTACK)检测试剂盒 人可溶性CD14分子(sCD14)检测试剂盒人免疫球蛋白E Fc段受体Ⅱ(FcεRⅡ/CD23)检测试剂盒 人白细胞分化抗原CD40配体(CD40L/TNFSF5)检测试剂盒人CD163分子(CD163)检测试剂盒人软骨糖蛋白39(GP39)检测试剂盒 人丛生蛋白(CLU)检测试剂盒人睫状神经营养因子(CNTF)检测试剂盒 人组织因子(TF)检测试剂盒人补体因子D(CFD)检测试剂盒 人Corin蛋白(CRN)检测试剂盒人C反应蛋白(CRP)检测试剂盒  人趋化因子C-X3-C-基元配体1(CX3CL1)检测试剂盒人生长调节致癌基因α(GROα/CXCL1)检测试剂盒  人上皮中性粒细胞活化肽78(ENA-78)检测试剂盒 人粒细胞趋化蛋白2(GCP-2)检测试剂盒人白介素8(IL-8)检测试剂盒人抗载脂蛋白抗体(AAHA)检测试剂盒人10kDa干扰素γ诱导蛋白(IP-10/CXCL10)检测试剂盒人干扰素诱导T细胞α亚族趋化因子 (I-TAC)检测试剂盒人基质细胞衍生因子1(SDF-1)检测试剂盒人B-淋巴细胞趋化因子(BLC)检测试剂盒 人趋化因子CXC配体16(CXCL16)检测试剂盒人胱抑素C(Cys-C)检测试剂盒人细胞色素C(Cyt-C)检测试剂盒人Dickkopf相关蛋白1 (DKK1)检测试剂盒人二肽基肽酶IV(DPP4)检测试剂盒人表皮生长因子(EGF)检测试剂盒人表皮生长因子受体(EGFR)检测试剂盒人内分泌腺来源的血管内皮生长因子(EG-VEGF)检测试剂盒 人内皮糖蛋白(ENG)检测试剂盒人内皮抑制素(ES)检测试剂盒人内皮素1(ET-1)检测试剂盒人内皮细胞蛋白C受体(EPCR)检测试剂盒人促红细胞生成素(EPO)检测试剂盒人凋亡相关因子(FAS/CD95)检测试剂盒 人凋亡相关因子配体(FASL/TNFSF6)检测试剂盒 人脂肪酸结合蛋白1(FABP1)检测试剂盒人甲胎蛋白(αFP)检测试剂盒 人酸性成纤维细胞生长因子(AFGF/FGF1)检测试剂盒人丙氨酸氨肽酶(AAP)检测试剂盒人成纤维细胞生长因子19(FGF19)检测试剂盒人成纤维细胞生长因子21(FGF21)检测试剂盒人FMS样酪氨酸激酶3配体(Flt3L)检测试剂盒 人卵泡抑素(FST)检测试剂盒 人铁调素(Hepc)检测试剂盒人生长停滞特异性蛋白6(Gas6)检测试剂盒人粒细胞集落刺激因子(G-CSF)检测试剂盒

胰岛素(INS)检测试剂盒适用生物     Mus musculus (Mouse，小鼠)    胰岛素(INS)检测试剂盒   检测范围     123.46-10000pg/mL     灵敏度     45.5pg/mL    样本类型     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids    实验时长     2.5h     实验方法     竞争抑制法    胰岛素(INS)检测试剂盒规格     96T    ELISA Kit for Insulin (INS)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!Organism species     Mus musculus (Mouse)    胰岛素(INS)检测试剂盒Sample type     Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids    Format     96-well strip plate    Assay length     2.5 hours    Detection range     123.46-10000pg/mL The standard curve concentrations used for the ELISA’s were 10000pg/mL, 3333.33pg/mL, 1111.11pg/mL, 370.37pg/mL, 123.46pg/mL    Sensitivity     The minimum detectable dose of this kit is typically less than 45.5pg/mL.    SpecificityThis assay has high sensitivity and excellent specificity for detection of Insulin (INS). No significant cross-reactivity or interference between Insulin (INS) and analogues was observed.RecoveryMatrices listed below were spiked with certain level of recombinant Insulin (INS) and the recovery rates were calculated by comparing the measured value to the expected amount of Insulin (INS) in samples. Matrix     Recovery range (%)     Average(%)    serum(n=5)     96-103     101    EDTA plasma(n=5)     89-97     94    heparin plasma(n=5)     99-105     102    PrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Insulin (INS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Insulin (INS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CVInter-Assay: CVLinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Insulin (INS) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Sample     1:2     1:4     1:8     1:16    serum(n=5)     94-102%     96-103%     99-105%     92-101%    EDTA plasma(n=5)     78-95%     82-97%     81-92%     85-97%    heparin plasma(n=5)     93-102%     78-88%     96-103%     78-102%    StabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials providedReagents     Quantity     Reagents     Quantity    Pre-coated, ready to use 96-well strip plate     1     Plate sealer for 96 wells     4    Standard     2     Standard Diluent     1×20mL    Detection Reagent A     1     Assay Diluent A     1×12mL    Detection Reagent B     1×120μL     Assay Diluent B     1×12mL    Reagent Diluent     1×300μL     Stop Solution     1×6mL    TMB Substrate     1×9mL     Instruction manual     1    Wash Buffer (30 × concentrate)     1×20mL    Assay procedure summary1. Prepare all reagents, samples and standards;2. Add 50μL standard or sample to each well.    And then add 50μL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37oC;3. Aspirate and wash 3 times;4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37oC;5. Aspirate and wash 5 times;6. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37oC;7. Add 50μL Stop Solution. Read at 450 nm immediately.Test principleThis assay employs the competitive胰岛素(INS)检测试剂盒 inhibition enzyme immunoassay technique. A monoclonal antibody specific to Insulin (INS) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Insulin (INS) and unlabeled Insulin (INS) (Standards or samples) with the pre-coated antibody specific to Insulin (INS). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Insulin (INS) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the 胰岛素(INS)检测试剂盒concentration of Insulin (INS) in the sample.