小鼠凝血因子Ⅷ（FⅧ）ELISA Kit

小鼠凝血因子Ⅷ（FⅧ）ELISA Kit

产品类型:

ELISA Kit

产品名称:

小鼠凝血因子Ⅷ（FⅧ）ELISA Kit

英文名称:

Mouse coagulation factor Ⅷ(FⅧ) ELISA Kit

货号:

HZ-E13084m

别名:

RP11-115M6.7, AHF, DXS1253E, F8B, F8C, FVIII, HEMA, coagulation factor VIII|coagulation factor VIIIc|factor VIII F8B

规格:

96T

种属:

Mouse

待测物名称:

coagulation factor VIII, procoagulant component

缩写:

F8

蛋白功能1:

Immunity

蛋白功能3:

Acute phase

检测范围:

0.312 ng/ml-20 ng/ml

灵敏度:

0.078 ng/ml

反应时间:

1-5h

所需样本体积:

50-100ul

检测波长:

450 nm

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FⅧ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FⅧ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FⅧ is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FⅧ bound in the initial step. The color development is stopped and the intensity of the color is measured.