大鼠细胞信号转导抑制因子3(SOCS3)检测试剂盒在临床检验中一般采用商品试剂盒进行测定。检测试剂盒大鼠细胞信号转导抑制因子3(SOCS3)检测试剂盒中有三个必要的试剂:免疫吸附剂、结合物和酶的底物等。完整的elisa试剂盒包含以下各组分:
(1)已包被抗原或抗体的固相载体(免疫吸附剂);
(2)酶标记的抗原或抗体(结合物);
(3)酶的底物;
(4)阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中);
(5)酶联物(结合物)及标本的稀释液;
(6) 大鼠细胞信号转导抑制因子3(SOCS3)检测试剂盒洗涤液;
(7)酶反应终止液。
酶与抗体交联的方法有许多种,根据酶的结构不同可采用不同的方法。对于制备HRP结合物,可用戊二醛二步法和过碘酸钠法。常用过碘酸盐氧化法,这种方法法只适用于含糖量较高的酶。过碘酸钠将HRP分子表面的多糖氧化为醛基,大鼠细胞信号转导抑制因子3(SOCS3)检测试剂盒醛基与抗体分子上的氨基形成Schiff碱而结合。后者可进一步用NaBH4(或乙醇胺)还原生成稳定的酶标记抗体。
大鼠细胞信号转导抑制因子3(SOCS3)检测试剂盒已包被抗原或抗体的固相载体在低温(2~8℃)干燥的条件下一般可保存6个月以上。有些不完整的试盒,仅供应包被用抗原或抗体,检测人员需自行包被。以下简述固相载体和包被过程。
HZ-EL-MK0465c 猴GDP解离抑制因子1(GDI1)检测试剂盒 Monkey GDI1 (GDP Dissociation Inhibitor 1) ELISA Kit
HZ-EL-MK0466c 猴微纤丝相关蛋白4(MFAP4)检测试剂盒 Monkey MFAP4 (Microfibrillar Associated Protein 4) ELISA Kit
HZ-EL-MK0467c 猴脂蛋白关联磷脂酶A2(LpPLA2)检测试剂盒 Monkey LpPLA2 (Phospholipase A2, Lipoprotein Associated) ELISA Kit
HZ-EL-MK0468c 猴Apelin 36(AP36)检测试剂盒 Monkey AP36 (Apelin 36) ELISA Kit
HZ-EL-MK0469c 猴α1抗胰蛋白酶(α1-AT)检测试剂盒 Monkey α1-AT (Alpha 1-Antitrypsin) ELISA Kit
HZ-EL-MK0470c 猴二氢嘧啶脱氢酶(DPYD)检测试剂盒 Monkey DPYD (Dihydropyrimidine Dehydrogenase) ELISA Kit
HZ-EL-MK0472c 猴硫酸皮肤素(DS)检测试剂盒 Monkey DS (Dermatan Sulfate) ELISA Kit
HZ-EL-MK0473c 猴成骨生长肽(OGP)检测试剂盒 Monkey OGP (Osteogenic Growth Peptide) ELISA Kit
HZ-EL-MK0474c 猴糖化蛋白(GSP)检测试剂盒 Monkey GSP (Glycosylated Serum Protein) ELISA Kit
HZ-EL-MK0476c 猴凋亡蛋白酶激活因子1(APAF-1)检测试剂盒 Monkey APAF-1 (Apoptosis Protease Activating Factor-1) ELISA Kit
HZ-EL-MK0477c 猴β淀粉样蛋白1-40(Aβ1-40)检测试剂盒 Monkey Aβ1-40 (Amyloid Beta Peptide 1-40) ELISA Kit
HZ-EL-MK0478c 猴补体片段3b受体(C3bR)检测试剂盒 Monkey C3bR (Complement Fragment 3b Receptor) ELISA Kit
HZ-EL-MK0479c 猴强啡肽(Dyn)检测试剂盒 Monkey Dyn (Big Dynorphin) ELISA Kit
HZ-EL-MK0480c 猴磷脂爬行酶2(PLSCR2)检测试剂盒 Monkey PLSCR2 (Phospholipid Scramblase 2) ELISA Kit
HZ-EL-MK0481c 猴低氧上调节因子1(HYOU1)检测试剂盒 Monkey HYOU1 (Hypoxia Up Regulated 1) ELISA Kit
HZ-EL-MK0482c 猴雄激素诱导蛋白1(AIG1)检测试剂盒 Monkey AIG1 (Androgen Induced Protein 1) ELISA Kit
HZ-EL-MK0484c 猴XV 型胶原(COL15)检测试剂盒 Monkey COL15 (Collagen Type XV) ELISA Kit
HZ-EL-MK0485c 猴白细胞分化抗原CD40配体(CD40L/TNFSF5)检测试剂盒 Monkey CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) ELISA Kit
HZ-EL-MK0487c 猴雌激素诱导蛋白PS2 (EIPS2)检测试剂盒 Monkey EIPS2 (Estrogen Induced Protein pS2) ELISA Kit
HZ-EL-MK0488c 猴凝血因子Ⅻ(F12)检测试剂盒 Monkey F12 (Coagulation Factor Ⅻ) ELISA Kit
HZ-EL-MK0489c 猴集蛋白亚家族成员11(COLEC11)检测试剂盒 Monkey COLEC11 (Collectin Sub-Family Member 11) ELISA Kit
HZ-EL-MK0490c 猴TATA框结合蛋白相关因子2(TAF2)检测试剂盒 Monkey TAF2 (TATA Box Binding Protein Associated Factor 2) ELISA Kit
HZ-EL-MK0491c 猴生长因子受体结合蛋白2(Grb2)检测试剂盒 Monkey Grb2 (Growth Factor Receptor Bound Protein 2) ELISA Kit
HZ-EL-MK0492c 猴维生素D结合蛋白(DBP)检测试剂盒 Monkey DBP (Vitamin D Binding Protein) ELISA Kit
HZ-EL-MK0493c 猴失调症蛋白质2(ATXN2)检测试剂盒 Monkey ATXN2 (Ataxin 2) ELISA Kit
HZ-EL-MK0494c 猴趋化因子C-C-基元配体16(CCL16)检测试剂盒 Monkey CCL16 (Chemokine C-C-Motif Ligand 16) ELISA Kit
HZ-EL-MK0496c 猴T细胞受体(TCR)检测试剂盒 Monkey TCR (Tcell Receptor) ELISA Kit
HZ-EL-MK0497c 猴睾酮受体(TR)检测试剂盒 Monkey TR (Testosterone Receptor) ELISA Kit