超快门宽增强型CCD相机

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LaVision GmbH

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PicoStar

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欧洲

北京欧兰科技发展有限公司

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第17年
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综合其他医疗/卫生生物产业石油/化工

NADPH中选择性探测多核形细胞中NADPH氧化酶检测方案(CCD相机)
NADPH oxidase (NOX2) is a multisubunit membrane-bound enzyme complex that, upon assembly in activated cells, catalyses the reduction of free oxygen to its superoxide anion, which further leads to reactive oxygen species (ROS) that are toxic to invading pathogens, for example, the fungus Aspergillus fumigatus. Polymorphonuclear cells (PMNs) employ both nonoxidative and oxidative mechanisms to clear this fungus from the lung. The oxidative mechanisms mainly depend on the proper assembly and function of NOX2. We identified for the first time the NAD(P)H-dependent enzymes involved in such oxidative mechanisms by means of biexponentialNAD(P)H-fluorescence lifetime imaging (FLIM). A specific fluorescence lifetime of 3670±140 picoseconds as compared to 1870 picoseconds for NAD(P)H bound to mitochondrial enzymes could be associated with NADPH bound to oxidative enzymes in activated PMNs. Due to its predominance in PMNs and due to the use of selective activators and inhibitors, we strongly believe that this specific lifetime mainly originates from NOX2. Our experiments also revealed the high site specificity of the NOX2 assembly and, thus, of the ROS production as well as the dynamic nature of these phenomena. On the example of NADPH oxidase, we demonstrate the potential of NAD(P)H-based FLIM in selectively investigating enzymes during their cellular function.
生物产业 2011-03-27
蛋白中双栖小泡蛋白和N-WASP调节肌蛋白组装的相互作用动力学检测方案(CCD相机)
Amphiphysin 1, an endocytic adaptor concentrated at synapses that couples clathrin-mediated endocytosis to dynamin-dependent fission,wasalsoshownto have a regulatory role in actindynamics. Here, we report that amphiphysin 1 interacts with N-WASP and stimulates N-WASP- and Arp2/3-dependent actin polymerization. Both the Src homology 3 and the N-BAR domains are requiredforthisstimulation.Acidicliposome-triggered,N-WASPdependent actin polymerization is strongly impaired in brain cytosol of amphiphysin 1 knock-out mice. FRET-FLIM analysis of Sertoli cells, where endogenously expressed amphiphysin 1 colocalizes with N-WASP in peripheral ruffles, confirmed the association between the two proteins in vivo. This association undergoes regulation and is enhanced by stimulating phosphatidylserine receptors on the cell surface with phosphatidylserine- containing liposomes that trigger ruffle formation. These results indicate that actin regulation is a key function of amphiphysin 1 and that such function cooperates with the endocytic adaptor role and membrane shaping/curvature sensing properties of the protein during the endocytic reaction.
医疗/卫生 2011-03-09
工业双频电容耦合射频放电,等离子体中空间和相位分辨等离子体参数检测方案(CCD相机)
The dynamics of high energetic electrons (11.7 eV) in a modified industrial confined dual-frequency capacitively coupled RF discharge (Exelan, Lam Research Inc.), operated at 1.937MHz and 27.118 MHz, is investigated by means of phase resolved optical emission spectroscopy. Operating in a He–O2 plasma with small rare gas admixtures the emission is measured, with one-dimensional spatial resolution along the discharge axis. Both the low and high frequency RF cycle are resolved. The diagnostic is based on time dependent measurements of the population densities of specifically chosen excited rare gas states. A time dependent model, based on rate equations, describes the dynamics of the population densities of these levels. Based on this model and the comparison of the excitation of various rare gas states, with different excitation thresholds, time and space resolved electron temperature, propagation velocity and qualitative electron density as well as electron energy distribution functions are determined. This information leads to a better understanding of the dual-frequency sheath dynamics and shows, that separate control of ion energy and electron density is limited.
其他 2011-03-07
混浊介质中混浊介质角域成像（ADI）实验中光传播时间门控和偏振优选效应检测方案(CCD相机)
Angular Domain Imaging (ADI) employs an angular filter array to accept photons within a small acceptance angle along the axis of an aligned laser light source and preferentially reject scattered light. Simulations show that the accepted photons travel the shortest paths between source and detector and are therefore the earliest to arrive. We fabricated angular filter arrays using silicon bulk micromachining and found that an array of 60 μm square shape microtunnels 1 cm in length accepted photons within 0.48 degree of axis of the micro-tunnels. This small acceptance angle rejected most of the scattered light and sub-millimeter resolution targets could be resolved in a few centimeters of turbid medium with at least six times reduced mean free path. ADI through media with higher scattering coefficients was not achievable due to unwanted acceptance of late arriving scattered photons. To reject the late arriving photons, we added time-domain filtration and linear polarization to ADI. The implementation of a time-gated camera, a 780 nm femtosecond pulsed laser, and linear polarization to our ADI system resulted in improved image contrast. The use of ADI with time-gating (gate width 250 ps) and linear polarization enabled visualization of sub-millimeter absorbing objects with approximately eight times higher image contrast compared to ADI in a scattering medium equivalent to six times reduced mean free path.
其他 2011-03-04

激光诱导水动力学和气泡中PIV和PDA实验研究检测方案(粒子图像测速)
Research on laser-liquid interaction and its induced fluid flows, as well as microbubbles, is important in many applications, such as laser ophthalmic microsurgery, manufacturing and repairing of micro-electronic-mechanical devices, laser deposition of thin liquid film to a specific location in micro system, etc. This work was focused on the interaction mechanisms of a laser pulse with distilled/degassed water as well as the characteristics of the microbubbles. Microbubbles and optohydrodynamic flows induced by a Nd:YAG pulse laser (New Wave Research) were studied. A recently improved PIV and PDA system were used to analyze the bubble dynamics and fluid flow quantitatively. Two CCD cameras were used to capture the images of microbubbles and visualize the laser induced Optohydrodynamic flows. The experimental results show that a bright fluid beam (density flow) with duration less than 127μs is produced by a focused laser pulse while an explosive cavitation just around the focus point is also occurred. Immediately following the fluid beam and the explosive cavitation, two new fluid regions which may be formed with the superheated dense fluid were found. One of them is just under the fluid beam due to the optical pressure while the other one is around the explosion area due to the force caused by the explosive cavitation. As shown in Figure 1, Single and multi microbubbles were generated immediately after the cavitation explosion when the nucleation conditions were satisfied. In addition, the characteristics, such as bubble velocities and diameters, were measured by a recently improved phase-Doppler anemometry (PDA). These results will help to have a better understanding of the mechanisms of laser-induced optohydrodynamic flows and bubbles related phenomena, which is crucial to numerical modeling.
其他 2016-10-24
高压（1-60巴）火焰中氮氧化合物组分定量激光诱导荧光成像检测方案(流量计)
Nitric oxide laser-induced-fluorescence (NO-LIF) 2-D imaging measurements using a new multi-spectral detection strategy are reported for high-pressure flames (1–60 bar). This work builds on previous research that identified interference LIF from O2 and CO2 in high-pressure flames and optimized the choice of excitation strategies as a function of application conditions. In this study, design rules are presented to optimize the LIF detection wavelengths for quantitative 2-D NO-LIF measurements over a wide range of pressures (1–60 bar) and temperatures. Simultaneous detection of LIF in multiple wavelength regions enables correction of the NO signal for interference from O2 and CO2 and allows simultaneous imaging of all three species. New experiments of wavelength-resolved 1-D LIF in slightly lean (/ = 0.9) and slightly rich (/ = 1.1) methane/air flames are used to evaluate the design rules and estimate the NO detection limits for a wide range of flame conditions. The quantitative 2-D measurements of NO in the burnt gas are compared with model calculations (using GRI-Mech 3.0) versus pressure for slightly lean and slightly rich flames. The discussions and demonstrations reported in this study provide a practical guideline for application of instantaneous 1-D or 2-D NO-LIF imaging strategies in high-pressure combustion systems. 2006 The Combustion Institute. Published by Elsevier Inc. All rights reserved.
其他 2016-10-24
落到倾斜箔片上的液体薄膜中流动的时间空间分辨热传导过程的实验研究检测方案(粒子图像测速)
This paper describes the development of an experimental technique that combines simultaneous planar laser-induced fluorescence (PLIF) and infrared (IR) thermography imaging, and its application to the measurement of unsteady and conjugate heat-transfer in harmonically forced, thin liquid-film flows falling under the action of gravity over an inclined electrically heated-foil substrate. Quantitative, spatiotemporally resolved and simultaneously conducted measurements are reported of the film thickness, film free-surface temperature, solid–liquid substrate interface temperature, and local/instantaneous heat flux exchanged with the heated substrate. Based on this information, local and instantaneous heat-transfer coefficients (HTCs) are recovered. Results concerning the local and instantaneous HTC and how this is correlated with the local and instantaneous film thickness suggest considerable heat-transfer enhancement relative to steady-flow predictions in the thinner film regions. This behaviour is attributed to a number of unsteady/mixing transport processes within the wavy films that are not captured by laminar, steady-flow analysis. The Nusselt number Nu increases with the Reynolds number Re; at low Re values the mean Nu number corresponds to 2.5, in agreement with the steady-flow theory, while at higher Re, both the Nu number and the HTC exhibit significantly enhanced values. Evidence that the HTC becomes decoupled from the film thickness for the upper range of observed film thicknesses is also presented. Finally, smaller film thickness fluctuation intensities were associated with higher HTC fluctuation intensities, while the amplitude of the wall temperature fluctuations was almost proportional to the amplitude of the HTC fluctuations.
其他 2016-10-17
湍流预混钝体火焰中引燃几率的模拟和实验研究检测方案(流量计)
The ignition characteristics of a premixed bluff-body burner under lean conditions were investigated experimentally and numerically with a physical model focusing on ignition probability. Visualisation of the flame with a 5 kHz OH* chemiluminescence camera confirmed that successful ignitions were those associated with the movement of the kernel upstream, consistent with previous work on non-premixed systems. Performing many separate ignition trials at the same spark position and flow conditions resulted in a quantification of the ignition probability Pign, which was found to decrease with increasing distance downstream of the bluff body and a decrease in equivalence ratio. Flows corresponding to flames close to the blow-off limit could not be ignited, although such flames were stable if reached from a richer already ignited condition. A detailed comparison with the local Karlovitz number and the mean velocity showed that regions of high Pign are associated with low Ka and negative bulk velocity (i.e. towards the bluff body), although a direct correlation was not possible. A modelling effort that takes convection and localised flame quenching into account by tracking stochastic virtual flame particles, previously validated for non-premixed and spray ignition, was used to estimate the ignition probability. The applicability of this approach to premixed flows was first evaluated by investigating the model’s flame propagation mechanism in a uniform turbulence field, which showed that the model reproduces the bending behaviour of the ST-versus-u curve. Then ignition simulations of the bluff-body burner were carried out. The ignition probability map was computed and it was found that the model reproduces all main trends found in the experimental study.
其他 2016-10-17

线粒体中多模光学成像检测方案(CCD相机)
采用LaVision公司独有的，快门宽度最小可达50皮秒的增强型CCD相机，通过化疗可咯和多模光学成像方法对光子激发诱导线粒体损伤机理进行了研究。
医疗/卫生 2017-07-24
组织,肿瘤中光学断层扫描检测方案(CCD相机)
An imaging fibre bundle is demonstrated for spatially-multiplexed probe beam delivery in OCT, with the aim of eliminating the mechanical scanning currently required at the probe tip in endoscopic systems. Each fibre in the bundle addresses a Fizeau interferometer formed between the bundle end and the sample, allowing acquisition of information across a plane with a single measurement. Depth scanning components are now contained within a processing interferometer external to the completely passive endoscope probe. The technique has been evaluated in our laboratory for non-biological samples. Images acquired using the bundle-based system are presented. The potential of the system is assessed, with reference to SNR performance and acquisition speed.
医疗/卫生 2016-10-24
蛋白中双栖小泡蛋白和N-WASP调节肌蛋白组装的相互作用动力学检测方案(CCD相机)
Amphiphysin 1, an endocytic adaptor concentrated at synapses that couples clathrin-mediated endocytosis to dynamin-dependent fission,wasalsoshownto have a regulatory role in actindynamics. Here, we report that amphiphysin 1 interacts with N-WASP and stimulates N-WASP- and Arp2/3-dependent actin polymerization. Both the Src homology 3 and the N-BAR domains are requiredforthisstimulation.Acidicliposome-triggered,N-WASPdependent actin polymerization is strongly impaired in brain cytosol of amphiphysin 1 knock-out mice. FRET-FLIM analysis of Sertoli cells, where endogenously expressed amphiphysin 1 colocalizes with N-WASP in peripheral ruffles, confirmed the association between the two proteins in vivo. This association undergoes regulation and is enhanced by stimulating phosphatidylserine receptors on the cell surface with phosphatidylserine- containing liposomes that trigger ruffle formation. These results indicate that actin regulation is a key function of amphiphysin 1 and that such function cooperates with the endocytic adaptor role and membrane shaping/curvature sensing properties of the protein during the endocytic reaction.
医疗/卫生 2011-03-09

鼠抗体中基于寿命的福斯特共振能量转移(FRET)参数检测方案(CCD相机)
采用德国LaVision公司独特的皮秒门宽增强型CCD相机对鼠抗体样本进行了增强器快门门宽尺度对基于寿命的福斯特共振能量转移(FRET)参数的估计的研究。
生物产业 2017-07-18
NADPH中选择性探测多核形细胞中NADPH氧化酶检测方案(CCD相机)
NADPH oxidase (NOX2) is a multisubunit membrane-bound enzyme complex that, upon assembly in activated cells, catalyses the reduction of free oxygen to its superoxide anion, which further leads to reactive oxygen species (ROS) that are toxic to invading pathogens, for example, the fungus Aspergillus fumigatus. Polymorphonuclear cells (PMNs) employ both nonoxidative and oxidative mechanisms to clear this fungus from the lung. The oxidative mechanisms mainly depend on the proper assembly and function of NOX2. We identified for the first time the NAD(P)H-dependent enzymes involved in such oxidative mechanisms by means of biexponentialNAD(P)H-fluorescence lifetime imaging (FLIM). A specific fluorescence lifetime of 3670±140 picoseconds as compared to 1870 picoseconds for NAD(P)H bound to mitochondrial enzymes could be associated with NADPH bound to oxidative enzymes in activated PMNs. Due to its predominance in PMNs and due to the use of selective activators and inhibitors, we strongly believe that this specific lifetime mainly originates from NOX2. Our experiments also revealed the high site specificity of the NOX2 assembly and, thus, of the ROS production as well as the dynamic nature of these phenomena. On the example of NADPH oxidase, we demonstrate the potential of NAD(P)H-based FLIM in selectively investigating enzymes during their cellular function.
生物产业 2011-03-27

TiO2中定位特性检测方案(CCD相机)
利用德国LaVision公司门宽达到皮秒量级的超快门宽增强型CCD相机PicoStar研究二氧化钛微粒聚集体的局域化行为。
石油/化工 2017-06-23