Agilent Bravo 自动液体处理平台

Agilent Bravo 自动化液体处理平台可以实施任意单相型脂质萃取方法，产生的样品适用于脂质组学 中的 LC/MS/MS 分析。使用 Agilent Bravo 的自动化处理可提高方法通量，并表现出始终如一的分析精度。本实验方案降低了样品前处理时间，从而大大减少脂质分析的成本/样品量。

Automated protocols to facilitate sample preparation in lipidomics have been previously developed. The aim of these procedures is to minimize human errors resulting from handling large quantities of samples, and to decrease the amount of time spent in sample preparation. This application note describes the semi-automated extraction of phospholipids and sphingolipids from human plasma using the Agilent Bravo Automated Liquid Handling Platform. This procedure includes the transfer of the starting material (plasma is usually delivered in 1.5-mL tubes) to 96-well plates, which can be either stored or used for the lipid extraction. In the latter case, a butanol/methanol (BuMe) mixture is added to the sample. Although the samples are still transferred manually to the sonicator and the centrifuge during the lipid extraction procedure, the reported protocol allows a high number of samples to be processed uniformly, decreasing the sample preparation time and yielding high reproducibility according to the results obtained after analysis of the extracted lipids with an Agilent UHPLC/MS/MS system.