Gator Anti-PEG 探针

Gator^® Anti-PEG 探针在光学层和表面化学做了创新设计，可以对 PEGylated LNPs 进行分析，不会出现传统 BLI 中观察到的信号反转问题。

QUANTITATION OF PEGYLATED LNPs

Binding curves and standard curve for PEGylated LNPs

 ( 1 μg/mL to 300 μg/mL in PBS+0.2% BSA )

QUANTITATION OF PEGYLATED LIPOSOMES

Binding curves and standard curve for PEGylated liposomes 

(1 μg/mL to 300 μg/mL in PBS + 0.2% BSA)

ANTI-PEG PROBE COMPATIBILITY WITH SERUM

Binding curves and standard curve for PEGylated LNPs in serum diluted 1:10 with PBS + 0.2% BSA

（ 1ug/ml to 100ug/ml in serum ）

BINDING KINETICS OF PEGYLATED LNP TO APOLIPOPROTEIN E ( Apo-E )

Binding between PEGylated LNP and Apo-E

( PEGylated LNP : 20 μg/mL. Apo-E : 2.5, 5, 10 and 20 nM )

参考文献

[1] Pilkington, Emily H et al. From influenza to COVID-19: Lipid nanoparticle mRNA vaccines at the frontiers of infectious diseases[J]. Acta biomaterialia vol. 131 (2021): 16-40.