Taq PCR Mix

Taq PCR Mix

1.产品介绍

预混的含有优化浓度的GenStar高纯度Taq DNA Polymerase、dNTPs、Mg2+、反应缓冲液和稳定剂等成分的2倍浓度的即用型PCR试剂。使用时只需加入DNA模板和引物，并用水补足体系至反应浓度（1×）。产品有含染料（红色）和不含染料（无色）两种选择，使用含染料的产品在 PCR 反应完成后，不需添加上样缓冲液即可直接上样进行电泳。针对丙烯酰胺凝胶检测，可选用for PAGE专用产品，背景更低。

2.产品列表

3.产品特点

直接上样：含染料产品PCR产物可直接上样进行凝胶电泳分析；

高度灵敏：高纯度的酶带来优良的灵敏性；

无基因组污染：扩增产物纯度高；

高效扩增：优化的缓冲体系发挥更强的扩增性能；

重复性好：体系预混合，减小加样误差，降低污染机会；

性能稳定：4℃保存3-6个月或室温（25℃）保存2-4周，反复冻融50 次，扩增性能无变化；

批次稳定：遵循标准化生产流程，经过严格的质量检测。

4.实验数据

1） 鼠尾样品扩增检测

鼠尾样品扩增检测。其中1-5为不同品牌产品，G1为GenStar A012批次1，G2为GenStar A012批次2。可见使用GenStar A012扩增不同引物条带清晰、扩增产物量高。

2） 水稻样品扩增检测

水稻样品扩增检测。其中P1-P7为不同产物大小扩增引物，可见使用GenStar A012扩增批间稳定，不同引物条带清晰、扩增产物量高。

3） 菌落PCR检测

 菌落PCR扩增检测。使用产品为GenStar A012。

4） PAGE凝胶电泳

聚丙烯酰胺凝胶电泳检测。使用产品为GenStar A006。

5.引用文献

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2. Pan Zhang, Biliang Zhang, Jian Jiao, et al. Modulation of Symbiotic Compatibility by Rhizobial Zinc Starvation Machinery. MBIO. 2020, Doi:10.1128/mBio.03193-19. IF: 5.94

3. Lili Yue, Liuqing Wang, Yangchun Du, et al. Type 3 Inositol 1,4,5-Trisphosphate Receptor is a Crucial Regulator of Calcium Dynamics Mediated by Endoplasmic Reticulum in HEK Cells. CELLS-BASEL. 2020, Doi:10.3390/cells9020275 IF: 5.77

4. YueXu, PingZhou, SenCheng, et al. A Bacterial Effector Reveals the V-ATPase-ATG16L1 Axis that Initiates. CELL. 2019. IF: 24.38

5. Jingke Xie, Weikai Ge, Nan Li, et al. Efficient base editing for multiple genes and loci in pigs using base editors. NAT COMMUN. 2019; 10: 2852.Doi:10.1038/s41467-019-10421-8 IF: 12.19

6. Ran-Ran Xing, Ran-Ran Hu, Jian-Xun Han, et al. DNA barcoding and mini-barcoding in authenticating processed animal-derived food: A case study involving the Chinese market. FOOD CHEM. 2019. IF: 5.399

7. Xinming Shen, Wei Liu, Yongjiu Chen, et al. Diagnostic significance of metallothionein members in recognizing cadmium exposure in various organs under low-dose exposure. CHEMOSPHERE. 2019. IF: 5.108

8. Lishi Li, Yulong Song, Xinrui Shi, et al. The landscape of miRNA editing in animals and its impact on miRNA biogenesis and targeting. Genome Res. 2018. IF: 10.101

9. Yushuai Wang, Weiqi Liang, Tian Tang, Constant conflict between Gypsy LTR retrotransposons and CHH methylation within a stress‐adapted mangrove genome. New Phytologist. 2018. Doi:10.1111/nph.15209 IF: 7.433

10. Yaqin Du, Ting Wang, Jun Xu, et al. Efficient derivation of extended pluripotent stem cells from NOD-scid Il2rg?/? mice. Protein & Cell. (2018). Doi:10.1007/s13238-018-0558-z IF: 6.228

11. Weili Liu, Ting Li, Pingzhang Wang, et al. LRRC25 plays a key role in all-trans retinoic acid-induced granulocytic differentiation as a novel potential leukocyte differentiation antigen. Protein & Cell. 2018. IF: 6.228

12. M. Wang, F. Liu,P.W. Crous, and L. Cai. Phylogenetic reassessment of Nigrospora: Ubiquitous endophytes, plant and human pathogens. Persoonia. 2017. Doi:10.3767/persoonia.2017.39.06 IF: 8.182

13. Ming-Hao Hu, Shuo-Bin Chen, Bo Wang, et al. Specific targeting of telomeric multimeric G-quadruplexes by a new triaryl-substituted imidazole. Nucleic Acids Research, 2017, Doi:10.1093/nar/gkw1195  IF: 10.162

14. Jie Wang, Tao Shen, Xiangbo Huang, et al. Serum hepatitis B virus RNA is encapsidated pregenome RNA that may be associated with persistence of viral infection and rebound. Journal of Hepatology. 2016. Doi:10.1016/j.jhep.2016.05.029  IF: 12.486

15. J Liu, N Sun, M Liu, et al; An autoregulatory loop controlling Arabidopsis HsfA2 expression: role of heat shock-induced alternative splicing. Plant Physiology, 2013.  IF: 6.841