Simoa助力HIV研究 | 首台HIV应用落地中国医科大学附属第一医院

最新研究表明[13]新一代临床前Quanterix p24试剂具有相较第四代Ag/Ab组合和独立p24  Ag分析更高的灵敏度，能够达到与标准核酸检测方法100%的敏感度，60个RNA拷贝（30个病毒/毫升）的检测灵敏度，同时拥有更少的样本处理时间，更加简单快速的运转周期，实现最早的HIV患者病情监测和干预。

近日，Quanterix在沈阳中国医科大学附属第一医院（下称附一院）进行了为期两天的全自动多重单分子免疫阵列分析仪HD-X装机以及HIV应用讲座，培训期间Quanterix技术团队详细解读仪器原理并演示操作流程，配合附一院研究人员和专家对HIV相关研究和疾病诊断进行上样检测，获得了各位研究员以及实验室主任对仪器性能和培训效果一致肯定。

中国医科大学附属第一医院是一所大型综合性三级甲等医院，也是国内外知名的区域性疑难急重症诊治中心，在国内外卫生界享有很高的声誉。作为辽宁省疑难急重症诊治中心，同时也是国家卫生计生委指定的东北三省唯一的全国11个国家级应急医疗救援中心之一和4个初级创伤救治中心之一，医院在抗击非典、抗击手足口病、防治流感等重大流行病中做出了突出贡献。此次Quanterix全自动多重单分子免疫阵列分析仪HD-X安装于附一院艾滋病研究重点实验室，立足科室服务全院，将为感染病类科研发展以及临床转化贡献坚实的力量。

目前HIV p24检测属于第四代p24 Ag/Ab组合或单独p24 Ag免疫分析法，问世已超过10年时间，已将诊断窗口期从3-4周缩短到2周左右，这些方法包括Abbott Architect HIV Ag/Ab combo EIA，Ortho Clinical Diagnostics Vitros HIV combo assay，Bio-Rad BioPlex 2200 HIV Ag-Ab combo assay，Bio-Rad Genscreen HIV-1 p24 Ag assay等，最新研究表明[13]新一代临床前Quanterix p24试剂具有相较第四代Ag/Ab组合和独立p24  Ag分析更高的灵敏度，能够达到与标准核酸检测方法100%的敏感度，60个RNA拷贝（30个病毒/毫升）的检测灵敏度，同时拥有更少的样本处理时间，更加简单快速的运转周期，实现最早的HIV患者病情监测和干预。在科研方面，Quanterix p24分析可检测从外周血分离的细胞或ART抑制的HIV患者组织中分离的细胞裂解物，以及猿猴-人类免疫缺陷病毒-感染的非人灵长类动物（NHPs）的细胞裂解物中定量低至1fg的p24蛋白，以得到胞内驱动转录、翻译和下游修饰的足够遗传完整的病毒信息，提供HIV感染和免疫反应之间的关联信息，为HIV治疗导向的临床试验提供强有力的理论基础。

Simoa检测HIV p24抗原示例

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